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abstracts. phytopharm 2o12
(EC50dpph = 0.071 ± 0.003 mg/ml). Both tomato waste ex- La = 0.51 ± 0.02 mg/ml). The obtained results show that
tracts influenced cell growth depending on cell line and tomato waste could represent a cheap and readily avail-
extract dose. The strongest activity against cancer cells able source of value-added ingredient for functional
was observed by Backa extract in HeLa cell line (EC50He- food and some other health products.
MICROPROPAGATION OF GLOSSOGYNE TENUIFOLIA CASSINI — A TRADITIONAL MEDICINAL PLANT
© Chang Hung-Chi-Kuo Chao-Lin2, Karuppaiya Palaniyandi3, Tsay Hsin-Sheng3 4
1Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung, Taiwan
2School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
3Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung, Taiwan
4Department of Agronomy, National Chung Hsing University, Taichung, Taiwan
Glossogyne tenuifolia Cass. (Hsiang Ju in chinese) belongs to the family Asteraceae and originates in Pen-ghu island, Taiwan and it is used to make traditional healthy food and drink consumed in Penghu island. G. tenuifolia has a long history of being use as an antipyretic, hepatoprotective and anti-inflammatory remedy in folk medicine among local residents. Oleanolic acid and lute-olin-7-glucoside are main active compound of G. tenuifolia. The levels of these active compounds in G. tenuifolia are affected by many factors including climatic change and pathogens. The plant cell culture is a promising potential alternative technique to produce high value sec-
ondary metabolites. Therefore, the present study aims to develop a micropropagation protocol for G. tenuifolia. Maximum numbers of shoots (2.4 shoots/explant) were obtained from in vitro seedling-derived shoot tip cultured on half-strength Murashige & Skoog (1962) (MS) medium supplemented with 0.1 mg/L benzyladenine. The well-developed shoots were rooted on half strength MS medium supplemented with 0.5 mg/L Napthaleneacetic acid and 0.1 mg/L putrescine. Eighty percent of the inoculated shoots produced roots with an average of 3.4 roots/shoot. The rooted plantlets were successfully acclimatized in greenhouse with 100 % survival rate.
ANTIOXIDANT ACTIVITY
OF KOELREUTERIA HENRYI DUMMER FLOWER
© Chen Jial-Jhen1, Chang Hung-Chi-23, Karuppaiya Palaniyandi3, Tsay Hsin-Sheng3 4
1School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan 2Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung, Taiwan 3Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung, Taiwan 4Department of Agronomy, National Chung Hsing University, Taichung, Taiwan
Koelreuteria henryi Dummer (Known as Golden Flame Tree in Taiwan) is a deciduous tree native to Taiwan belonging to the family Sapindaceae. it has been traditionally used in folk medicine for the treatment of diarrhea, malaria and urethritis. The present study was undertaken to find the antioxidant properties of the flower of Koelreuteria henryi Dummer by DPPH radical-scavenging activity, Trolox equivalent antioxidant activity, and ferric reducing antioxidant power assay and phytochemical analysis. The flowers were extracted with five different polar solvents: distilled deionized
water, methanol, ethanol, ethyl acetate and hexane. Maximum yield of antioxidant components were observed in water extract (27.2 %) followed by ethanol extract (20.2 %o) and methanol extract (19.5 %o). it was also found that methanol extract showed highest radical scavenging activity (83.5 ± 0.3) at the concentration of 0.25 mg/ml. Phytochemical analysis showed that highest concentration of total phenol and total fla-vonoids were observed in methanol extract. The results revealed that the flower of K. henryi could be exploited as potential antioxidant additives.
ТОМ 10/2012/2 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] И ^^ 39
