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abstracts. phytopharm 2012
of iL-10 (as immunosupressive cytokine) and iL-6 (which takes part in myoproteosis degradation) serum levels can restore immune cells activity. it was correlated with increasing the leukocyte integrins expression. Down regulation of serum iL-6 and iL-10 cytok-
ines in males of CBA inbred mice with high frequency of spontaneous hepatocarcinomas is considered to reduce antitumour antibodies production (which cover tumor cells antigens and diminish immune cells activity) as well as cachexia symptoms.
NUTRACEUTICALS FROM BEETROOT POMACE EXTRACT WITH ANTIOXIDANT AND HEPATOPROTECTIVE EFFECTS
© Canadanovic-Brunet Jasna-1, Cebovic Tatjana2, Vulic Jelena 1, Cetkovic Gordana1, Djilas Sonja 1
1Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia 2Faculty of Medicine, Clinical centre of Vojvodina, Hajduk Veljkova 3, Novi Sad, Serbia
The beetroot pomace (BPE), residue from the juice processing, is promising source of bioactive compounds with antioxidant, hepatoprotective and antitumor properties. The aim of this research was to investigate the antioxidant activity of BPE and its effect on biochemical parameters of oxidative stress evaluated in liver homogenate of rats. The antioxidant activity of BPE, including reducing power and DPPH free radical scavenging activity were determined spectrophotometrically. The effects of BPE on several biochemical parameters of oxidative stress were evaluated in liver homogenate of rats treated with CCl4 as a free radical generator: LPx intensity, content of GSH and activities of GSH-Px, GR, Px, XOD and CAT BPE was able to scavenge DPPH radicals (EC50DPPH=3.4 ig/ml). The reducing power of BPE increased with increasing the concentration. The BPE, combined with a CCl4, expressed
a clear and dose-dependent suppressive effect against lipid peroxidation in liver homogenate. Simultaneous application of extract and CCl4 has led to a significant decrease in GSH-Px levels, keeping the GSH content in liver homogenate close to physiological level. Similar impact on GR activity has been observed in liver of the animals. The catalase activity was decreased after treatment with BPE and CCl4 Similar influence on hepatic Px activity has been also observed. Although CCl4 does not alter the activity of hepatic XOD certain increase of XOD activity has been measured after addition of BPE. Obtained results demonstrated that the beetroot pomace extract might possess a specific antioxidant potential, suggesting that it might be used not only as a hepatoprotective agent, but as an active agent in treatment of other disorders caused by oxidative stress.
VALORISATION OF ANTIOXIDANT AND CELL GROWTH ACTIVITIES OF TOMATO WASTE LIPOPHILIC EXTRACTS
© Cetkovic Gordana-1, Savatovic Sladjana 1, Canadanovic-Brunet Jasna 1, Djilas Sonja1, Mandic Anamarija2, Cetojevic-Simin Dragana3
1Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
2Institute for Food Technology in Novi Sad, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia
3oncology Institute of Vojvodina, Institutskiput4, 21204 Sremska Kamenica, Serbia
Tomato, fresh and its products, possess a high nutritional value, due to content of different types of anti-oxidants: carotenoids, polyphenolics, vitamin C and vitamin E. According to the fact that different valuable compounds remained in the waste from tomato juice processing, the aim of this research was to investigate the antioxidant and cell growth activities of tomato waste lipophilic extracts (obtained from genotypes — Backa and Saint Pierre). The lycopene and P-carotene in tomato waste extracts were identified and quantified by HPLC method by matching their retention times (RT) and on-line ultraviolet (UV) spectra with those of stan-
dards. The antioxidant activity of extracts were investigated by measuring 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity by spectrophoto-metric method. The cell growth activity was measured using the sulforhodamine B colorimetric assay on human cancer HeLa (cervix epitheloid carcinoma) and MCF7 (breast adenocarcinoma) cell lines. The Baka waste extract contained higher st content of lycopene (13.6 ± 0.1 mg/g) and P-carotene (12.0 ± 0.3 mg/g). The extracts were able to scavenge DPPH radical and higher antioxidant activity, expressed as iC50DPPH value, was obtained in the case of the Backa waste extract
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Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy]
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abstracts. phytopharm 2o12
(EC50dpph = 0.071 ± 0.003 mg/ml). Both tomato waste ex- La = 0.51 ± 0.02 mg/ml). The obtained results show that
tracts influenced cell growth depending on cell line and tomato waste could represent a cheap and readily avail-
extract dose. The strongest activity against cancer cells able source of value-added ingredient for functional
was observed by Backa extract in HeLa cell line (EC50He- food and some other health products.
MICROPROPAGATION OF GLOSSOGYNE TENUIFOLIA CASSINI — A TRADITIONAL MEDICINAL PLANT
© Chang Hung-Chi-Kuo Chao-Lin2, Karuppaiya Palaniyandi3, Tsay Hsin-Sheng3 4
1Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung, Taiwan
2School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
3Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung, Taiwan
4Department of Agronomy, National Chung Hsing University, Taichung, Taiwan
Glossogyne tenuifolia Cass. (Hsiang Ju in chinese) belongs to the family Asteraceae and originates in Pen-ghu island, Taiwan and it is used to make traditional healthy food and drink consumed in Penghu island. G. tenuifolia has a long history of being use as an antipyretic, hepatoprotective and anti-inflammatory remedy in folk medicine among local residents. Oleanolic acid and lute-olin-7-glucoside are main active compound of G. tenuifolia. The levels of these active compounds in G. tenuifolia are affected by many factors including climatic change and pathogens. The plant cell culture is a promising potential alternative technique to produce high value sec-
ondary metabolites. Therefore, the present study aims to develop a micropropagation protocol for G. tenuifolia. Maximum numbers of shoots (2.4 shoots/explant) were obtained from in vitro seedling-derived shoot tip cultured on half-strength Murashige & Skoog (1962) (MS) medium supplemented with 0.1 mg/L benzyladenine. The well-developed shoots were rooted on half strength MS medium supplemented with 0.5 mg/L Napthaleneacetic acid and 0.1 mg/L putrescine. Eighty percent of the inoculated shoots produced roots with an average of 3.4 roots/shoot. The rooted plantlets were successfully acclimatized in greenhouse with 100 % survival rate.
ANTIOXIDANT ACTIVITY
OF KOELREUTERIA HENRYI DUMMER FLOWER
© Chen Jial-Jhen1, Chang Hung-Chi-23, Karuppaiya Palaniyandi3, Tsay Hsin-Sheng3 4
1School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan 2Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung, Taiwan 3Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Taichung, Taiwan 4Department of Agronomy, National Chung Hsing University, Taichung, Taiwan
Koelreuteria henryi Dummer (Known as Golden Flame Tree in Taiwan) is a deciduous tree native to Taiwan belonging to the family Sapindaceae. it has been traditionally used in folk medicine for the treatment of diarrhea, malaria and urethritis. The present study was undertaken to find the antioxidant properties of the flower of Koelreuteria henryi Dummer by DPPH radical-scavenging activity, Trolox equivalent antioxidant activity, and ferric reducing antioxidant power assay and phytochemical analysis. The flowers were extracted with five different polar solvents: distilled deionized
water, methanol, ethanol, ethyl acetate and hexane. Maximum yield of antioxidant components were observed in water extract (27.2 %) followed by ethanol extract (20.2 %o) and methanol extract (19.5 %o). it was also found that methanol extract showed highest radical scavenging activity (83.5 ± 0.3) at the concentration of 0.25 mg/ml. Phytochemical analysis showed that highest concentration of total phenol and total fla-vonoids were observed in methanol extract. The results revealed that the flower of K. henryi could be exploited as potential antioxidant additives.
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