Agerelated and gender features of circulating immune complexes and lipid metabolismin inflammatory bowel diseases Текст научной статьи по специальности «Клиническая медицина»

UDC 616.344.002+577.115/316.346 DOI: 10.22141/2308-2097.53.1.2019.163456

M.V. Stoikevych, I.A. Klenina, O.M. Tatarchuk, V.A. Karachinova, N.S. Fedorova

SI"Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine", Dnipro, Ukraine

Age-related and gender features of circulating immune complexes

and lipid metabolism in inflammatory bowel diseases

For cite: Gastroenterology. 2019;53(l):39-43. doi: 10.22141/2308-2097.53.1.2019.163456_

Abstract. Background. The purpose of the present work was to assess the level of circulating immune complexes (CIC) and lipid metabolism state in patients with inflammatory bowel disease (IBD). Materials and methods. We examined 80 patients with IBD: 53 with ulcerative colitis (UC) and 27 with Crohn's disease (CD). Depending on the gender, they were divided into two groups: 40 women and 40 men. Also, depending on the age, all patients were divided into three groups: young (45 individuals — 28 with UC and 17 with CD); middle-aged (15 persons — 10 with UC and 5 with CD); elderly (20 patients — 15 with UC and 5 with CD). The control group included 20 apparently healthy individuals. In patients' blood serum, the levels of CIC, total cholesterol, triglycerides, phospholipids, high-density, low-density and very low-density lipoprotein cholesterol were determined, as well as atherogenic index. Results. Serum level of CIC significantly exceeded the normal values in women with IBD — by 1.57 times (p < 0.05), which can be considered as an indicator of inflammation intensity, involvement of the immune system in the pathological process. In women with UC, the content of CIC was significantly increased — by 1.62 times (p < 0.05) compared to the control group. In IBD, the level of CIC depended on the age of patients; it was significantly increased — by 1.5 times (p < 0.05) and 1.4 times (p < 0.05) — in middle-aged patients and in the elderly, respectively, compared to a group of young patients. The correlation between the level of CIC and the age (r = +0.552; p = 0.025) was established. The violation of lipid metabolism was observed in women with UC, which manifested in 1.7-time reduction of serum triglycerides (p < 0.001); in men with CD, a decrease in this indicator by 1.5 times (p < 0.05) occurred compared to the control group. In women with UC, the serum level of very low-density lipoproteins was 2 times lower (p < 0.05), and in men with CD — 1.8 times (p < 0.05) as compared to controls. A probable reduction in the content of triglycerides was detected: by 1.5 times (p < 0.05) and 1.6 times (p < 0.05) in young patients with UC and CD, respectively, and by 1.6 times (p < 0.05) in middle-aged patients with CD. In parallel, there was a pronounced tendency to cholesterol lowering in all young patients with IBD. A probable decrease in the atherogenic index was detected in young patients. Conclusions. The completed studies allowed establishing and confirming the presence of biochemical changes in patients with IBD (lipid metabolism and the content of CIC), which play the main role in the pathogenetic mechanisms of these diseases.

Keywords: inflammatory bowel diseases; ulcerative colitis; Crohn's disease; circulating immune complexes; lipid metabolism

Орипнальт досл^ження Original Researches < ■ i ГАСТРОЕНТЕРОЛОПЯ GASTROENTEROLOGY

Патолопя кишечника / Bowel Pathology

Introduction

Inflammatory bowel diseases (IBD), which include ulcerative colitis (UC) and Crohn's disease (CD), remain one of the most complex problems of gastroenterology. Constant recurrent course with a progressive violation of the structure and functions of the intestinal mucosa is accompanied by a significant decrease in the patient's quality

of life that makes IBD one of important medical and social problems and involves search for new directions in studying the etiology and pathogenesis in order to improve methods for the prevention and treatment of this pathology [1, 2]. The continued interest of scientists in this pathology is associated with an increase in IBD morbidity all over the world. According to some scientists, this situation is due to

© «Гастроентеролопя» / «Гастроэнтерология» / «Gastroenterology» («Gastroenterologia»), 2019 © Видавець Заславський О.Ю. / Издатель Заславский А.Ю. / Publisher Zaslavsky O.Yu., 2019

Для кореспонденци: Стойкевич М.В., ДУ «1нститут гастроентерологи НАМН УкраТни», пр. Слобожанський, 96, м. Днтро, 49074, Укра'ша; e-mail: stoykevich.marina@gmail.com

For correspondence: M.V. Stoikevych, State Institution "Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine', Slobozhanskii Avenue, 96, Dnipro, 49074, Ukraine; e-mail:

stoykevich.marina@gmail.com; contact phone: +380504807038

anthropogenic pollution of the biosphere and environment, urbanization, growing influence of adverse external factors, number of allergens in food, high degree of sensitization of the population, dietary habits, widespread use of alcohol and other factors, and it leads to an increase not only in the frequency of IBD, but also in the number of severe cases, common forms of the disease, extraintestinal lesions and complications of IBD [3].

In the last decade, the extension of methodological possibilities, the introduction of functional and morphological methods of research have greatly deepened the understanding of the etiology and pathogenesis of chronic intestinal diseases and made it possible to develop recommendations for their diagnosis and treatment. However, many issues of clinical and pathogenetic features of the course of this nosology are not fully resolved, the effectiveness of treatment is insufficient that contributes to the formation of resistant forms of diseases, decrease in the quality of life and loss of efficiency. This encourages the search for the mechanisms of intestinal pathology, which can be associated with extra-organic causes [4, 5].

It is known that the basis of IBD pathogenesis is a violation of the immune response, which leads to the development of nonspecific inflammation in the intestinal walls and mucosa. The effect of circulating immune complexes (CIC) and inflammatory mediators (cytokines, histamine, reactive oxygen species, nitric oxide) on the cells of the intestinal wall lead to its damage and tissue degradation [6, 7].

Violation of the intestinal mucosal barrier, increased antigen loading, damage to the cytokine link of homeosta-sis, activation of immune cells, excess synthesis of CIC can cause the formation of extraintestinal lesions in IBD [8].

CIC is the physiological product of the antigen-antibody reaction, which is a part of the protective immune mechanisms of the body. The formation of immune complexes is an integral indicator of the development of humoral immune response. Some content of immune complexes should be constantly present in the blood for the implementation of physiological processes of homeostasis. Moreover, this indicates an adequate body response to external influences. But it should be noted that with excessive accumulation of CIC due to increased production or lack of their removal from the body, a transition to the pathology is possible that is caused by an increase in the biological activity of immune complexes, and, under special conditions, they can play an important role in the pathogenesis of autoimmune processes [5].

The role of lipids in the physiological processes and in the development of pathological conditions, together with many other factors, depends on their qualitative composition. These characteristics are determined by many processes: synthesis, catabolism, plasma elimination, qualitative features of protein and lipid components. The state of these processes, in turn, is due to the condition of the digestive tract, especially the liver as one of the main organs that regulates the substrate-enzymatic relationship in lipid metabolism [6, 9]. Previously, we have shown that in patients with IBD, the total content of free fatty acids in the blood serum was significantly increased as compared to controls, precisely at the expense of polyunsaturated fatty acids, especially in

middle-aged patients, which may be due to the activation of inflammatory processes, immune responses, and also to the enhanced lipid peroxidation [9].

The purpose of the work was to assess the level of circulating immune complexes and lipid metabolism state in patients with inflammatory bowel diseases.

Materials and methods

Eighty patients with IBD (53 with UC and 27 with CD) were examined at the department of bowel diseases of the Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine in 2017—2018.

Depending on the gender, patients were divided into two groups: 40 women and 40 men. Also, depending on the age, all patients were divided into three groups: young (45 individuals — 28 with UC and 17 with CD); middle-aged (15 persons — 10 with UC and 5 with CD); elderly (20 patients — 15 with UC and 5 with CD). The control group included 20 apparently healthy individuals.

The level of CIC in patients' blood serum was determined according to V. Haskova technique.

Such biochemical parameters as total cholesterol (TC), triglycerides (TG), phospholipids, high-density (HDL), low-density (LDL) and very low-density lipoprotein (VLDL) cholesterol, atherogenic index (AI) were analysed with Cormay test-kits (Poland) and biochemical analyzer Stat Fax 1904 Plus (Awareness Technology, USA) and calculated according to Friedewald formula. The normal TC levels were 2.96-4.4 mmol/l, TG — 0.40-0.9 mmol/l, HDL — 0.99-1.59 mmol/l, LDL — 1.63-2.59 mmol/l and VLDL — 0.22-0.40 mmol/l.

Descriptive statistics was used for statistical analysis of the data; the comparison of the mean values of variables was carried out using parametric methods (Student's t-test) for the normal distribution of the data measured on the interval scale. The correspondence of the characteristics of distribution to the normal distribution law was verified using the Shapiro-Wilk test. In other cases, a non-parametric method was used (Mann-Whitney U-test). To compare the particle distribution of two or more variables, the x2 test was used. Correlation analysis was performed by Pearson (for data measured on the interval scale) and Spearman coefficients (for data not measured on the interval scales). All calculations were performed in SPSS 9.0 for Windows.

Results and discussion

The content of the CIC in patients with IBD was 0.8-14.5 optical density units. The average levels were (4.82 ± 0.42) optical density units in men and (5.37 ± 0.49) optical density units in women. The CIC level in 65.0 % of women and in 57.5 % of men with IBD exceeded that of control group. There is no statistically significant difference between the level of CIC in men and women.

When analysing the indicator depending on the nosology, it was found that in women with IBD, the level of CIC was significantly increased compared to the control group — by 1.57 times (p < 0.05). In women with UC, the level of CIC was significantly increased — by 1.62 times (p < 0.05) compared to the control group.

40

Gastroenterology, ISSN 2308-2097 (print), ISSN 2518-7880 (online)

Vol. 53, No. 1, 2019

The analysis of indicators was performed depending on the age of patients (Fig. 1). A possible increase in the serum level of CIC was observed in 80.0 % of middle-aged and in 70.0 % of elderly patients, indicating active inflammatory process. The highest level of CIC was found in the group of middle-aged patients with UC (a 1.9-time increase compared to controls, p < 0.05).

It was found that the level of CIC increased with increasing age of patient, which was confirmed by the established correlation between the level of CIC and age (r = +0.552; p = 0.025). So, their level is probably increased by 1.5 times (p < 0.05) and 1.4 times (p < 0.05) in middle-aged patients and in the elderly with IBD, respectively, in comparison with the group of young patients.

In the general group of patients with IBD (men and women, young and middle-aged patients), the following changes in serum lipid metabolism were observed: a probable decrease in TG content by 1.5 times ((0.76 ± 0.06) mmol/l vs. (1.15 ± 0.12) mmol/l, p < 0.05), LDL — by 1.8 times ((0.36 ± 0.05) mmol/l vs. (0.65 ± 0.14) mmol/l, p < 0.05) and a tendency to decrease in the content of TC, HDL, LDL compared with controls.

The results of biochemical studies of lipid metabolism indicators in the blood serum of patients with IBD depending on gender are presented in Table 1. As you can see, there was a probable decrease in serum TG level of women in the general group of IBD — by 1.6 times ((0.71 ± 0.07) mmol/l vs. (1.15 ± 0.12) mmol/l, p < 0.01), and in UC — by 1.7 times ((0.66 ± 0.07) mmol/l vs. (1.15 ± 0.12) mmol/l, p < 0.001). In men with IBD, especially with CD, there was also a probable decrease in the TG content — by 1.4 times ((0.84 ± 0.09) mmol/l vs. (1.15 ± 0.12) mmol/l, p < 0.05) and by 1.5 times, respectively ((0.78 ± 0.11) mmol/l vs. (1.15 ± 0.12) mmol/l, p < 0.05). Probably, a decrease in TG level may indirectly indicate a violation of the cell membrane structure and malabsorption of these fats in the small intestine.

A clear downward trend in HDL serum levels was determined in men with IBD, unlike women, whose indicator was slightly different from the control group (Table 1). Decreased HDL levels indicate a violation of the transport of excess lipids from the blood to the liver.

A probable difference in the content of LDL that play a major role in the formation of atherosclerotic plaques was not observed in patients with IBD compared to the control

*

71 * 6 -

5 -

4 -

3 -

2 -

1 -

0 -I-^-1-®-1-

Young Middle-aged Elderly

■ UC ■ CD ■ Control

Figure 1 — Serum level of CIC in patients with IBD depending on the age and nosology

Notes: * — р < 0.05 compared to the control group, * — р < 0.05 — compared to the young patients.

group. Analysis of nosologies showed similar changes in both UC and CD (Table 1).

A probable 2-fold decrease in the serum content of VLDL ((0.32 ± 0.03) mmol/l vs. (0.65 ± 0.14) mmol/l, p < 0.05) was determined in women with IBD and 2.2fold — in women with UC ((0.30 ± 0.03) mmol/l vs. (0.65 ± 0.14) mmol/l, p < 0.05). In men with CD, there was a probable 1.8-time decrease in VLDL content ((0.36 ± 0.05) mmol/l vs. (0.65 ± 0.14) mmol/l, p < 0.05). They transport 5—10 times more TG than cholesterol esters. The risk of atherosclerosis developing increases with increased levels of VLDL and LDL in the blood, as well as with low HDL contents.

AI was significantly lower in women with IBD — by 1.2 times ((2.13 ± 0.15) mmol/l vs. (2.55 ± 0.09) mmol/l, p < 0.05), with UC — by 1.3 times ((1.99 ± 0.16) mmol/l vs. (2.55 ± 0.09) mmol/l, p < 0.01) compared to controls. In men, AI changes were non-significant. AI is an integral indicator that allows you to accurately predict the risk of atherosclerosis developing. High atherogenicity is determined by the presence of LDL fraction. HDL fraction is antiathe-rogenic.

There was a probable decrease in the serum TG levels in young patients with IBD — by 1.5 times ((0.75 ± 0.07) mmol/l vs. (1.15 ± 0.12) mmol/l, p < 0.05) and in middle-aged patients with CD — by 1.6 times ((0.73 ± 0.12) mmol/l

Table 1 — Lipid metabolism in patients with inflammatory bowel disease depending on gender, M ± m

Indicator Controls (n = 20) Women Men

IBD (n = 40) UC (n = 27) CD (n = 13) IBD (n = 40) UC (n = 26) CD (n = 14)

TG, mmol/l 1.15 ± 0.12 0.71 ± 0.07** 0.66 ± 0.07*** 0.81 ± 0.17 0.84 ± 0.09* 0.87 ± 0.12 0.78 ± 0.11*

TC, mmol/l 5.20 ± 0.60 4.35 ± 0.18 4.19 ± 0.24 4.69 ± 0.24 4.23 ± 0.21 4.30 ± 0.560 4.08 ± 0.28

HDL, mmol/l 1.49 ± 0.12 1.43 ± 0.05 1.43 ± 0.06 1.44 ± 0.08 1.30 ± 0.05 1.31 ± 0.06 1.29 ± 0.10

LDL, mmol/l 3.10 ± 0.50 2.60 ± 0.16 2.46 ± 0.22 2.88 ± 0.22 2.55 ± 0.18 2.60 ± 0.24 2.43 ± 0.20

VLDL, mmol/l 0.65 ± 0.14 0.32 ± 0.03* 0.30 ± 0.03* 0.37 ± 0.08 0.39 ± 0.071 0.40 ± 0.06 0.36 ± 0.05*

AI 2.55 ± 0.09 2.13 ± 0.15* 1.99 ± 0.16** 2.41 ± 0.31 2.34 ± 0.16 2.38 ± 0.22 2.24 ± 0.18

Notes: the probability of changes compared to the control group: * — p < 0.05; ** — p < 0.01; *** — p < 0.001.

vs. (1.15 ± 0.12) mmol/l, p < 0.05) against the general downward trend in TG content in these age groups compared to controls.

It is important to note a probable TG decrease in middle-aged patients with CD by 1.6 times ((0.70 ± 0.12) mmol/l vs. (1.15 ± 0.12) mmol/l, p < 0.05). In the blood of elderly patients, there were no significant changes in TG content in contrast to controls, but it tended to increase compared to young and middle-aged.

There were no significant changes in the content of TC, HDL, LDL in patients of all age groups. A probable 1.9-time decrease was noted in VLDL of young patients with IBD, UC and CD, and 2-fold — in the middle-aged patients with IBD and CD compared to the control group.

AI was significantly lower in young patients (2.04 ± 0.10; 1.96 ± 0.12 vs. 2.55 ± 0.09, p < 0.001, respectively, for IBD and UC) as compared to controls. In middle-aged patients, there was a tendency to AI increase, especially in persons with UC. In middle-aged patients and in the elderly, AI was within the normal range.

The results show that in patients with IBD, the level of CIC depended on age, so in patients of middle and advanced age, it was probably higher than in young ones, and a high level of CIC indicated that the macrophage system does not eliminate the antigen-antibody complex.

Thus, the detected changes of total serum lipids indicate metabolic disorders and the presence of energy insufficiency regardless of gender or age characteristics, which may be due to disorders of neutral fats digestion and malabsorption of food products in the small intestine. A 1.2-time decrease in the total serum cholesterol in the general group of patients with IBD allowed assuming that there are violations in the structure of cell membranes.

The completed studies allowed establishing and confirming the presence of biochemical changes (lipid metabolism and the content of CIC) in patients with inflammatory bowel diseases that play the main role in the pathogenetic mechanisms of these diseases, have their clinical manifestations and need therapeutic correction.

Conclusions

1. Serum level of CIC significantly exceeded the normal values in women with IBD — by 1.57 times (p < 0.05), which can be considered as an indicator of inflammation intensity, involvement of the immune system in the pathological process. In women with UC, the level of CIC was significantly increased — by 1.62 times (p < 0.05) compared to the control group. In IBD, the level of CIC depended on the age of these patients. Its level is significantly increased — by 1.5 times (p < 0.05) and 1.4 times (p < 0.05) in middle-aged patients and in the elderly, respectively, compared to a group of young patients. The correlation between the level of the CIC and the age (r = +0.552; p = 0.025) was established.

2. The violation of lipid metabolism was observed in women with ulcerative colitis, which manifested in 1.7-time reduction in the serum triglycerides (p < 0.001), and in men with Crohn's disease, there was a 1.5-time decrease in this indicator (p < 0.05) compared to the control group. In women with ulcerative colitis, the level of very low-density

lipoproteins was 2 times lower (p < 0.05), and in men with Crohn's disease — 1.8 times (p < 0.05) lower compared to controls.

3. There was a probable reduction in triglyceride content — by 1.5 times (p < 0.05) and 1.6 times (p < 0.05) in young patients with ulcerative colitis and Crohn's disease, respectively, and by 1.6 times (p < 0.05) in middle-aged patients with Crohn's disease. In parallel, there was a clear downward trend to cholesterol lowering in all young patients with inflammatory bowel diseases. A probable decrease in the atherogenic index is determined in young patients.

Conflicts of interest. Authors declare no conflicts of interests that might be construed to influence the results or their manuscript.

References

1. Boiko TI, Stoikevich MV, Kolbasina EV, Sorochan EV. The state of the function of the vascular endothelium in patients with chronic inflammatory diseases. Modern Gastroenterology. 2010;(51):5-10. (in Russian).

2. Stepanov YuM, Skyrda IYu, Petishko OP. Chronic inflammatory bowel diseases: epidemiological features in Ukraine. Gastroenterologia. 2017;51(2):11-19. doi: 10.22141/2308-2097.51.2.2017.101703. (in Ukrainian).

3. Nosova IA. Difficulties in diagnosing and substantiating the principles of basic treatment of patients with non-specific inflammatory bowel diseases. Modern Gastroenterology. 2007;(36):51-55. (in Ukrainian).

4. Pasijeshvili LM, Andrusha AB. Chronic colitis as a factor in the development and progression of secondary osteoporosis in patients with spinal osteochondrosis. Modern Gastroenterology. 2008;(40):30-33. (in Ukrainian).

5. Vdovychenko VI, Kornijchuk OP, Merencova OO, Losynsky JuS. Comparative characteristics of intestinal mi-crobiocenosis in patients with irritable bowel syndrome and ulcerative colitis. Modern Gastroenterology. 2010;(54):67-70. (in Ukrainian).

6. Tatianina OF, Potapov AS, Namazova LS, Tsimba-lova YeG, Kucherenko AG, Surkov AN. Markers of the enteric inflammation in the event of intestinal diseases: literature review. Pediatricheskaya Farmakologiya. 2008;5(3):39-45. (in Russian).

7. Stepanov YuM, Fedorova NS. Fecal calprotectin content in patients with chronic inflammatory bowel disease. 2010;(52):44-48. (in Russian).

8. Dorofjejev AE, Dragomyrycka NV, Izha GM, Rosso-hina OO. Affection of hepatobilialry system in ulcerative colitis and Crohn's diseas. University clinic. 2013;9(2):228-230. (in Ukrainian).

9. Kalinkina NV. Osobennosti metabolizma oksida azota pod vliianiem razlichnykh kumuliativnykh doz antratsiklinovykh preparatov. Diss. kand. med. nauk [Features of the metabolism of nitric oxide under the influence of various cumulative doses of anthracycline drugs. PhD diss.]. Donetsk; 2010. 6p. (in Russian).

Received 27.01.2019 ■

42

Gastroenterologia, ISSN 2308-2097 (print), ISSN 2518-7880 (online)

Vol. 53, No. 1, 2019

Стойкевич М.В., Кленна I.A., Татарчук О.М., Карачинова В.А., Федорова Н.С. ДУ «Нститут гастроентерологП НАМН Украни», м. Днпро, Украна

BiKOBi i гендерн особливост циркулюючих iмунних KOMnAeKciB та ладного MeTa6oAi3My при запальних захворюваннях кишечника

Резюме. Мета: оцiнити рiвень циркулюючих iмунних комплексiв (Ц1К) i стану лiпiIдного обмiну в пащенпв iз вь ковими та гендерними особливостями перебiгу запальних захворювань кишечника (ЗЗК). Матерiалu та методи. Проведено обстеження 80 пащенпв iз ЗЗК: 53 iз неспецифiчним виразковим колггом (НВК) та 27 iз хворобою Крона (ХК). Вони були роздшеш на двi групи залежно вщ статi: 40 чоло-втв та 40 жiнок. Крiм того, ум хворi були подiленi на групи залежно вщ вiку: молодi (45 осiб — 28 iз НВК та 17 iз ХК); середнього вшу (15 пацieнтiв — 10 iз НВК та 5 iз ХК); по-хилого вшу (20 ошб — 15 iз НВК та 5 iз ХК). Контрольну групу становили 20 практично здорових ошб. У сироватщ кровi визначали рiвень Ц1К, загального холестерину, три-глiцеридiв, холестерину лшопроте'1шв високо'1, низько! та дуже низько! щшьносп (ЛПДНЩ), коефiцieнт атероген-ностi. Результати. Рiвень Ц1К у сироватцi кровi вiрогiIдно перевищував норму в жшок iз ЗЗК — у 1,57 раза (р < 0,05), що можна розглядати як показник штенсивносп запален-ня, залучення iмунно! системи до патолопчного процесу. У жшок iз НВК рiвень Ц1К вiрогiдно пщвищений — у 1,62 раза (р < 0,05) порiвняно з контрольною групою. При ЗЗК умют Ц1К залежав вщ вiку хворих, вш був вiрогiIдно пщви-щений — у 1,5 раза (р < 0,05) та 1,4 раза (р < 0,05) у пащенпв

середнього та похилого вшу вщповщно порiвняно з молоди-ми хворими. Встановлено кореляцш м1ж рiвнем Ц1К та вь ком (г = +0,552; р = 0,025). Виявлено порушення лшщного обмiну в жшок iз НВК, що виражалося в зниженш вмiсту триглiцеридiв у сироватщ кровi в 1,7 раза (р < 0,001), а у чо-ловтв iз ХК вщбувалося зменшення цього показника в 1,5 раза (р < 0,05) порiвняно з контрольною групою. У жшок iз НВК вiрогiIдно знижувався рiвень ЛПДНЩ в сироватщ кровi — у 2 рази (р < 0,05), а в чоловтв iз ХК — у 1,8 раза (р < 0,05) порiвняно з контрольною групою. Визначено вiро-пдне зниження вмiсту триглiцеридiв — у 1,5 раза (р < 0,05) та в 1,6 раза (р < 0,05) у молодих пащенпв iз НВК та ХК вщповщно та в 1,6 раза (р < 0,05) у пащенпв середнього вшу з ХК. Паралельно спостериалася виражена тенденцiя до зменшення вмюту холестерину у всгх молодих пащенпв iз ЗЗК. Визначено вiрогiIдне зниження коефЩента атероген-ностi в молодих хворих. Висновки. Проведет дослщження дозволили встановити та пщтвердити наявнiсть бiохiмiчних змiн з боку лiпiIдного обмшу та вмiсту Ц1К у пащенпв iз за-пальними захворюваннями кишечника. Ключовi слова: запальш захворювання кишечника; неспе-цифiчний виразковий коли; хвороба Крона; циркулюючi iмуннi комплекси; лiпiIдний обмiн

Стойкевич М.В., Кленина И.А., Татарчук О.М., Карачинова В.А., Федорова Н.С. ГУ «Институт гастроэнтерологии НАМН Украины», г. Днепр, Украина

Возрастные и гендерные особенности циркулирующих иммунных комплексов и липидного метаболизма при воспалительных заболеваниях кишечника

Резюме. Цель: определить уровень циркулирующих иммунных комплексов (ЦИК) и состояние липидного обмена у пациентов с возрастными и гендерными особенностями протекания воспалительных заболеваний кишечника (ВЗК). Материалы и методы. Проведено обследование 80 пациентов с ВЗК: 53 с неспецифическим язвенным колитом (НЯК) и 27 с болезнью Крона (БК). Они были разделены на две группы в зависимости от пола: 40 мужчин и 40 женщин. Кроме того, все больные были разделены на группы в зависимости от возраста: молодые (45 человек — 28 с НЯК и 17 с БК); среднего возраста (15 пациентов — 10 с НЯК и 5 с БК); пожилые (20 человек — 15 с НЯК и 5 с БК). Контрольную группу составили 20 практически здоровых людей. В сыворотке крови определяли уровень ЦИК, общего холестерина, триглицеридов, холестерина липопротеинов высокой, низкой и очень низкой плотности (ЛПОНП), коэффициент атерогенности. Результаты. Уровень ЦИК в сыворотке крови достоверно превышал норму у женщин с ВЗК — в 1,57 раза (р < 0,05), что можно рассматривать как показатель интенсивности воспаления, вовлечения иммунной системы в патологический процесс. У женщин с НЯК уровень ЦИК достоверно повышен — в 1,62 раза (р < 0,05) по сравнению с контрольной группой. При ВЗК содержание ЦИК зависело от возраста больных, оно было достоверно повышено — в 1,5 раза (р < 0,05) и 1,4 раза (р < 0,05)

у пациентов среднего и пожилого возраста соответственно в сравнении с молодыми больными. Установлена корреляция между уровнем ЦИК и возрастом (г = +0,552; р = 0,025). Выявлено нарушение липидного обмена у женщин с НЯК, что выражалось в снижении уровня триглицеридов в сыворотке крови в 1,7 раза (р < 0,001), а у мужчин с БК происходило уменьшение данного показателя в 1,5 раза (р < 0,05) по сравнению с контрольной группой. У женщин с НЯК достоверно снижался уровень ЛПОНП в сыворотке крови — в 2 раза (р < 0,05), а у мужчин с БК — в 1,8 раза (р < 0,05) по сравнению с контрольной группой. Установлено достоверное уменьшение уровня триглицеридов — в 1,5 раза (р < 0,05) и в 1,6 раза (р < 0,05) у молодых пациентов с НЯК и БК соответственно и в 1,6 раза (р < 0,05) у пациентов среднего возраста с БК. Параллельно наблюдалась выраженная тенденция к снижению уровня холестерина у всех молодых пациентов с ВЗК. Установлено достоверное уменьшение коэффициента атерогенно-сти у молодых больных. Выводы. Проведенные исследования позволили установить и подтвердить наличие биохимических изменений со стороны липидного обмена и уровня ЦИК у пациентов с воспалительными заболеваниями кишечника. Ключевые слова: воспалительные заболевания кишечника; неспецифический язвенный колит; болезнь Крона; циркулирующие иммунные комплексы; липидный обмен