CC BY
45
УДК 591.69.7
Van Ha Nguyen1, Van Tang Nguyen1,2, Duy Ngo HA1 and Aleksei Ermolenko3
department of Parasitology, Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet street, Hanoi, Vietnam 2 Hai Duong Medical Technical University, 1 Vu Huu street, Hai Duong city, Vietnam 3Department of Parasitology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far
Eastern Branch of Russian Academy of Sciences, 159 Stoletiya Street, Vladivostok, 690022
A NEW TREMATODE SPECIES, BIANIUM TONKINENSIS N. SP. (DIGENEA, LEPOCREADIIDAE), FROM HEADRABBIT PUFFER LAGOCEPHALUS LUNARIS (BL OCH & SCHNEIDER, 1801) IN THE GULF OF TONKIN, VIETNAM
A new species of Lepocreadiidae, Bianium tonkinensis n. sp., is described from Headrabbit Puffer Lagocephalus lunaris (Bloch & Schneider, 1801) in the Gulf of Tonkin, Vietnam. In addition, Bianium Stunkard, 1930 were reported for the first time from Vietnam. B. tonkinensis n. sp. is distinguished from its congeners in having ovary with 8-11 separate subglobular follicles.
Key words: Lepocreadiidae, Bianium tonkinensis, Gulf of Tonkin, Vietnam.
Ван Xa Нгуен1, Ван Танг Нгуен1,г, Зуй Нго Ха1 и Алексей Ермоленко 3 НОВАЯ РАЗНОВИДНОСТЬ ТРЕМАТОД BIANIUM TONKINENSIS N.SP. (DIGENEA, LEPOCREADIIDAE) ОТ КАШТАНОВОГО ИГЛОБРЮХА LAGOCEPHALUS LUNARIS (БЛОХ И ШНЕЙДЕР, 1801) В ТОНКИНСКОМ ЗАЛИВЕ
РЕСПУБЛИКИ ВЬЕТНАМ
Новая разновидность Lepocreadiidae, Bianium tonkinensis была описана по каштановому иглобрюху Lagocephalus lunaris (Блох и Шнейдер, 1801) в Тонкинском заливе в Республике Вьетнам. Стоит добавить, что в 1930 г. во Вьетнаме впервые было сообщено о Bianium Stunkard. Новый вид Bianium tonkinensis n.sp. отличается от своих сородичей наличием завязей с 8-11 разделенными сублобуляр-ными фолликулами.
Ключевые слова: Lepocreadiidae, Bianium tonkinensis, Тонкинский залив, Вьетнам.
Introduction
In their checklist of Vietnamese fish parasites, Arthur and Bui (2006) listed fourteen lepocreadiid species, of which nine species had been described by Oshmarin (1965), Oshmarin et al. (1961), Mamaev (1970) and Parukhin (1966) from Vietnamese waters. At present, eight species of Lepocreadiinae have been reported from the Gulf of Tonkin, namely Callogonotrema fistulariae Oshmarin 1965; Hypocreadium cavum Bray and Cribb, 1966; Hypocreadium scaphosomum (Manter, 1940); Multitestis magnacetabulum Mamaev, 1970; Opechona formiae Oshmarin, 1965; Trigonotrema alatum Goto and Ozaki, 1929 and some unname 'Opechona-type' species Hypocreadium sp.; Lepocreadium sp. (Oschmarin, 1965; Mamaev, 1970; Parukhin, 1971, 1989). In this paper we report a genera, Bianium Stunkard, 1930 for the first time from Vietnam with descriptions of a new species, Bianium tonkinensis n. sp. In addition, a key to species of Bianium is also provided.
Materials and methods
Digeneans collected from freshly killed fish were fixed by being pipetted into nearly boiling saline and immediately preserved in 70 % ethanol. Whole-mounts were stained with Mayer's acid carmine, de- stained in 4 % HC1 in 70 % ethanol, dehydrated in ascending concentrations of ethanol, cleared in graduated (increasing) concentrations of xilen in 100 % ethanol to 100 % xilen, and finally whole mounted in Canada balsam.
Measurements (in micrometer) are expressed as ranges with means in parentheses. Length is given before the width. Specimens were deposited in the Vietnam National Museum of Nature (VNMN).
Results
Fifteen specimens of Headrabbit Puffer Lagocephalus lunaris (Bloch & Schneider, 1801) in The Gulf of Tonkin, Vietnam, measuring from 19.5 to 23.0 cm in standard length (mean 21.5 cm), were netted and examined for parasites in Octorber 2013. Of these, three fish were infected with 39 specimens of the new species of Bianium. Family Lepocreadiidae Odhner, 1905 Subfamily Lepocreadiinae Odhner, 1905 Bianium Stunkard, 1930 Bianium tonkinensis sp. n. (fig. 1-3) Description:
Based on 21 specimens. Body elongate oval. Tegument spinous; spines embedded in tegument, with short scale-like extremity protruding, reach to close to posterior extremity, sparser posteriorly. Scoop incomplete, not joined posteriorly; lateral flaps with spread lateral small patches in forebody (fig. 1, 3). Oral sucker oval, subterminal. Ventral sucker oval, smaller oral sucker. Prepharynx entirely within posterior concavity of oral sucker. Pharynx oval. Oesophagus straight. Caeca reach to posterior end where they terminate abutting against body wall; no evidence of ani seen.
Testes 2, subglobular, entire, tandem, contiguous, oblique. External seminal vesicle saccular, reaching to about level of ovary. Male terminal genitalia of 'Opechona-type' (fig. 2). Cirrus-sac reaching well into hindbody. Internal seminal vesicle oval. Pars prostatica oval, vesicular, lined with anuclear cell-like bodies. Ejaculatory duct long, muscular, folded. Genital atrium distinct. Genital pore just post-bifurcal, sinistrally submedian.
Ovary immediately pretesticular, medial, consists of about 8-11 separate subglobular follicles. Seminal receptacle large, oval, sinistral to ovary or oblique to testes. Laurer's canal opens dorsally to seminal receptacle. Uterus preovarian, intercaecal, mainly in anterior hindbody but reaching sin-istrally to ventral sucker. Eggs tanned, operculate. Metraterm muscular, about third length of cirrus-sac, enters genital atrium from left. Vitellarium follicular; fields reach from bifurcal level to posterior extremity, ventral, present laterally, confluent in forebody and post-testicular region.Excretory pore terminal. Vesicle wide, reaches almost to bifurcal level. Taxonomic summary
Type host: Headrabbit Puffer Lagocephalus lunaris (Bloch & Schneider, 1801) (family Tetraodontidae)
Type locality: The Gulf of Tonkin, Cat Ba Island, Vietnam (200 45'N; 1070 05'E). Site of infection: Intestine.
Type specimens: VNMN holotype No. 2013, and paratypes No. 2014-2020. Etymology: The new species is named after its type locality. Discussion
Bray (2005) mentioned two diagnostic characteristics of the genus Bianium Stunkard, 1930, viz. 'Opechona-type' cirrus-sac and the scoop or lateral flanges incomplete in the anterior third of the body. Our specimens key to Bianiumin Bray (2005). The genus Bianium have seven currently valid species:
B. plicitum (Linton, 1928) from the USA, Australia, India, China waters
B. hemistoma (Ozaki, 1928) from the northwest of Pacific (Japan, China)
B. indicum (Gupta, 1967) from Indian ocean
B. purii Gupta, 1968 from Indian ocean
B. rewa Bray, Cribb &Barker, 1996 from Australia
B. sponggiosum Bray & Cribb, 1998 from Australia
B. tonkinensis n. sp. from the Gulf of Tonkin, Vietnam
Key to species of Bianium
1a. Anterior scoop absent; lateral flaps spread lateral patches in forebody .................................
............................................................................................B. sponggiosum Bray & Cribb, 1998
lb. Anterior scoop present; lateral edges of anterior part of body folded ventrally
to form scoop .....................................................................................................................................2
2a. Vitelline follicles restricted to small lateral patches at level of cirrus-sac..............................
..............................................................................................................B. indicum (Gupta, 1967)
2b. Vitelline follicles reach to posterior extremity .....................................................................3
3a. Genital pore sinistral at level of mid-ventral suckeror posterior margin of ventral sucker ...4
3b. Genital pore adjacent to antero-sinistral margin of ventral sucker........................................5
4a. Ovary with 12-28 lobes...............................................................B. hemistoma (Ozaki, 1928)
4b. Ovary trilobed................................................................B. rewa Bray, Cribb &Barker, 1996
5a. Ovary with 8-11 lobes.............................................................................B. tonkinensis n. sp.
5b. Ovary with 20-23 lobes ........................................................................................................6
6a. Eggs shorter than 50 pm.........................................................................B. purii Gupta, 1968
6b. Eggs longer than 50 pm.................................................................B. plicitum (Linton, 1928)
Host Lagocephalus lunaris
n 10
Length 976-1,656 (1,296)
Width 320-488 (406)
Oral sucker 84-120 (106) x 96-132 (112)
Ventral sucker 64-128 (94) x 60-112 (92)
Sucker-ratio 1:0.54-1,12 (0,84)
Forebody 240-436 (319)
Pharynx 56-100 (73) x 48-104 (80)
Oral sucker: pharynx width ratio 1:1,12-2,33 (1,43)
Oesophagus 12-80 (29)
IB to AE 188-348 (260)
Anterior testis 140-240 (184) x 112-200 (146)
Posterior testis 140-268 (226) x 44-188 (145)
Post-testicular region 124-400 (258)
Cirrus-sac 144-284 (228) x 66-108 (88)
Genital pore to AE 232-432 (310)
Ovary 84-196 (140) x 144-212 (178)
Number of ovary lobes 8-11 (10)
Vitellarium to AE 240-432 (322)
Eggs 48-68 (58) x 32-54 (44)
Width %* 26-37 (32)
Forebody %* 19-28 (25)
Intestinal bifurcation to AE %* 17,4-25.0 (20,4)
Post-testicular region %* 13-25 (19)
Genital pore to AE %* 17,1-27,9 (24,0)
Table 1. Dimensions of Bianium tonkinensis n. sp. * of body-length.
Abbreviations: AE, anterior extremity; Acknowledgements
This research is funded by the Project of Basic Research, grant number VAST.DA47.12/16-19
Fig. 1-3. Bianium tonkinensis n. sp. 1 - Ventral view of the holotype. 2 - Male terminal genitalia. Scale-bar: 500 цш, 100 цш. 3 - Ventral view of the paratype. Scale-bar: 500 цш
References
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