CC BY
18
34 • "PROTIST—2016
might represent new deeply-branching lineages of microbial eukaryotes, currently without any cultured representatives or morphological information.
THE PLASTID GENOMES OF THE CRYPTO-MONAD ALGAE REVEAL LINEAGE-SPECIFIC GENE LOSS
Kim J.I.1. Yi G.2, Yoon H.S.3, Shin W.1
1 - Department of Biology, Chungnam National University, Daejeon, Korea
2 - Department of Computer Science, Gangneung-Wonju National University, Wonju, Korea
3 - Department of Biological Sciences, Sungkyunkwan University, Suwon, Korea
shinw@cnu.ac.kr
Cryptophytes are an enigmatic group of photo-synthetic unicellular eukaryotes with plastids derived from red alga through secondary endosymbiosis that contains four different genomes (host nuclear. mitochondrial. plastid, and nucleomorph) in a cell. They are comprised of brown-, red-, or blue/ green photosynthetic species. as well as colorless nonphotosynthetic species. Although its complex evolutionary history, the origins of the host and endosymbiont components in cryptophyte algae are poorly understood. Here, we report a comparative analysis of plastid genomes from six cryptophyte genera. Three newly determiend cryptomonads plastid genomes of Chroomonas, Cryptomonas, and Storeatula share a number of features including gene arrangement and gene content with previously reported ones of Rhodomonas, Teleaulax and Guil-lardia. The plastid genomes of cryptomonads reveal lineage-specific gene loss and intron insertion in eight significant gene syntenic positions. The chlL/ chlN/chlB genes, which is the light-independent (dark active) protochlorophyllide oxidoreductase (LIPOR), show the evolutionary changes across the cryptomonad lineages. We will discuss phylogenetic relationships of the red-derived lineages using 93 plastid genes showing a strong monophyletic group of the cryptophytes and haptophytes.
A NEW SOIL CILIATE, BIROJIMIA SOYAEN-SIS NOV. SPEC. (CILIOPHORA: UROSTYLI-DA) FROM SOUTH KOREA Kim Kang-San1, Jung Jae-Ho2, Min Gi-Sik3
1 - Department ofBiological Sciences, Inha University, 100Inha-ro, Nam-gu, Incheon 22212
2 - Department of Biology, Gangneung-Wonju National University, Jukheon-gil, Gangneung-si, Gangwon-do, 25457, South Korea
3 - Department ofBiological Sciences, Inha University, 100Inha-ro, Nam-gu, Incheon 22212, South Korea mingisik@inha.ac.kr
A new soil urostylid ciliate, Birojimia soyaensis nov. spec., was discovered from Soya Island, Incheon, South Korea. It is described the species based on live and stained specimen observation, and 18S ribosomal RNA gene sequence analyses. Birojimia soyaensis nov. spec. is characterized by the following features: body slender elongate and somewhat twisted; 170—200 ^m * 40—50 ^m body size in vivo; contractile vacuole located at middle of left cell margin; cortical granules present; 37—48 adoral membranelles; 3 frontal and 2 frontoterminal cirri present; III/2 and buccal cirrus present; midventral pairs only; pretransverse ventral and transverse cirri present; 1 left and 4 right marginal rows, including 3 compound rows; 5 complete dorsal kineties with additional 3 shortened kineties which are anteriorly of compound rows; 8—11 caudal cirri; 53—69 macronuclear nodules; and 2 or 3 micronuclei. Birojimia soyaensis nov. spec. is similar to B. terricola, but is distinguished by cortical granules (present vs. absent), number of caudal cirri (8—11 vs. 2—7) and number of dorsal bristle rows (8 vs. 6, on average). Birojimia muscorum is separated from B. terricola and B. soyaensis n. sp. by midventral row present (vs. absent, midventral pairs only) which is an important key of the Bakuellidae. Furthermore, in phylogenetic tree, Birojimia soyaensis has widely distance from the bakuellids. Thus, we agree with Berger (2006) that the new genus needs to establish for B. muscorum. Phylogenetic tree suggests this new species is the most closely related to the genus Hemicycliostyla.
FIRST MARINE PHOTOSYNTHETIC TESTATE AMOEBA CONTAINING THE CHRO-MATOPHORE: PAULINELLA LONGICHRO-MATOPHORA Kim S.1, Park M.G.2
1 - Department of Oceanography, Pukyong National University, Busan, Republic of Korea
2 - Department ofOceanography, Chonnam National University, Gwangju, Republic of Korea mpark@chonnam.ac.kr
Of 10 formally described species in the genus, all species are marine heterotrophic species, except for one freshwater species, Paulinella chromatophora which is the sole species to have plastids termed the "chromatophores" of a Synechococcus/Prochloro-coccus-like cyanobacterial origin. Here, we report the first marine phototrophic species, Paulinella longichromatophora sp. nov., using light and electron microscopy and molecular data. This new species contains two blue-green U-shaped plastids reaching up to 40 ^m in length. Further, the new Paulinella species is characterized by having five oral scales
