CC BY
2*
ALT'23 The 30th International Conference on Advanced Laser Technologies
B-I-5
VIS-NIR diffuse reflectance spectroscopy system with self-calibrating
fiber-optic probe
V. Perekatova, A. Kostyuk, M. Kirillin, E. Sergeeva, D. Kurakina, O. Shemagina, A. Orlova,
A. Khilov and I. Turchin
Federal Research Center A. V. Gaponov-Grekhov Institute of Applied Physics of the Russian Academy of Sciences,
603950 Ulyanov St., 46, Nizhny Novgorod, Russia
ilya340@mail.ru
Diffuse reflectance spectroscopy (DRS) is based on illuminating biological tissue with broadband light in the visible (VIS) and/or near infrared (NIR) spectrum region and detecting backscattered light at a given distance from the source. The recorded spectrum contains information on the absorption of various tissue chromophores (oxy- and deoxyhemoglobin, melanin, water, lipids), the concentration of which can be reconstructed by solving an inverse problem. One of the key points of successful reconstruction of tissue chromophores is taking into account the instrumental characteristics of the DRS system. Traditional ratiometric (or single-slope) approach based on the measurements with two source-detector distances (one source and two detectors or two sources and a single detector) allows for compensation of detector spectral sensitivity and the source brightness variations [1], but it does not allow compensating all transient functions of the source and detector. The self-calibrating approach proposed in [2] is based on symmetrical multi-distance measurements (at least four measurements with two sources and two detectors) and makes it possible to compensate for the instrumental contributions of the source and detector channels. Additionally, it is less sensitive to changes in the optical coupling between the optical sensor and tissue [3] in comparison to single-distance and single-slope approaches. In this paper, we present an experimental setup for VIS-NIR DRS with a fiber optic probe using a self-calibrating approach. To our knowledge, this is the first application of the self-calibration approach for the 460-1030 nm ultra-wideband (VIS-NIR) DRS. The stability of the self-calibrating and traditional single-slope approaches to instrumental perturbations were compared in phantom and in vivo studies on human palm, including attenuations in individual channels, fiber curving, and introducing optical inhomogeneities in the probe-tissue interface [4] . The self-calibrating approach demonstrated high resistance to instrumental perturbations introduced into the source and detection channels, while the single-slope approach showed resistance only to perturbations introduced into the source channels. The developed experimental setup has been employed successfully in the in vivo studies on rats to reveal the differences in dynamics of allo- and autografts physiological parameters (blood and water content, and oxygenation) [5].
There are many applications of DRS in biomedicine, among them the study of brain hemodynamics, diagnosis of skin diseases, assessing the tumor boundaries of various localizations, and many other applications.
The study was supported by Center of Excellence «Center of Photonics» funded by The Ministry of Science and Higher Education of the Russian Federation, Contract No. 075-15-2022-316
[1] F. Scholkmann, A. J. Metz, and M. Wolf, "Measuring tissue hemodynamics and oxygenation by continuous-wave functional near-infrared spectroscopy—how robust are the different calculation methods against movement artifacts?," Physiological measurement 35, 717 (2014).
[2] D. M. Hueber, S. Fantini, A. E. Cerussi, and B. B. Barbieri, "New optical probe designs for absolute (self-calibrating) NIR tissue hemoglobin measurements," in Optical tomography and spectroscopy of tissue III, (SPIE, 1999), 618-631.
[3] A. Sassaroli, G. Blaney, and S. Fantini, "Dual-slope method for enhanced depth sensitivity in diffuse optical spectroscopy," JOSA A 36, 1743-1761 (2019).
[4] V. Perekatova, A. Kostyuk, M. Kirillin, E. Sergeeva, D. Kurakina, O. Shemagina, A. Orlova, A. Khilov, I. Turchin, VIS-NIR Diffuse Reflectance Spectroscopy System with Self-Calibrating Fiber-Optic Probe: Study of Perturbation Resistance, Diagnostics, 13, 457 (2023).
[5] I. Turchin, V. Beschastnov, P. Peretyagin, V. Perekatova, A. Kostyuk, A. Orlova, N. Koloshein, A. Khilov, E. Sergeeva, M. Kirillin, M. Ryabkov, Multimodal Optical Monitoring of Auto- and Allografts of Skin on a Burn Wound, Biomedicines, 11, 351 (2023).
