CC BY
72023, Scienceline Publication
Worlds Veterinary Journal
World Vet J, 13(1): 222-233, March 25, 2023
DOI: https://dx.doi.org/10.54203/scil.2023.wvj24
Toxic Effects of Nanographene Oxide on Testes of Rats
Ehsan Faraj Abd-Alsahib* © , and Satar Abood Faris
Department of Biology, College of Education for Pure Sciences, University of Thi-Qar, Iraq *Corresponding author's Email: [email protected]
ABSTRACT
The current study aimed to examine the effects of nanographene oxide on the testes. A total of 48 male albino rats were randomly divided into 6 groups. The first, second, third, fourth, and sixth groups were treated with graphene oxide nanopowder at 20, 30, 40, 50, and 60 mg/kg concentrations, respectively. The sixth group was considered the control group. The results indicated a significant decrease in the average testis weight of rats treated with different nanographene oxide dosages, compared to the control group. There was also a significant decrease in the level of FSH and testosterone of treated rats with nanographene oxide, while there was no significant difference in the level of LH hormone when compared to the control group. The histological examination of the testes in the treated rats indicated hemorrhage, decreased sperm count, decreased thickness of the tubular epithelium, dissociation of connective tissue between the seminiferous tubules, in addition to hematological congestion, necrosis of the tubular epithelium, divergence of the seminal tubules, absence of sperm, shattering of the seminal tubule wall and degeneration sperm-forming cells and edema formation. Using the transmission electron microscope, the findings revealed a range of cellular changes, such as the presence of two-headed spermatids, the destruction of the nucleus membrane, spermatoblasts, the destruction of the cell membrane, and the denting of the nucleus membrane. It can be concluded that the nanographene oxide at 20-60 mg/kg concentrations can have harmful effects on spermatogenesis and normal function testis in rats.
Keywords: Laboratory rat, Nanographene oxide, Testes, Toxic effect INTRODUCTION
Graphene is the thinnest electronic material which possesses distinctive chemical and physical properties. Graphene has remarkable properties, such as high surface area, thermal conductivity, electrical conductivity, and mechanical strength. These unique properties have led to an explosion of recent research in its composition, characterization, and development of its applications, especially in electronic devices, transparent electrodes for solar cells, plasma screens, and energy storage devices (Dhiman and Dhamija, 2014). The two-dimensional allotropic structure and bio-inherent properties of graphene make it applicable for biomedical and therapeutic purposes (Priyadarsini et al., 2018). The derivatives of graphene, such as graphene oxide, have received great attention due to their excellent solubility in physiological media, their good biocompatibility at the level of human exposure, and their ability to combine with other nanomaterials (Markovic et al., 2011). Graphene oxide has been widely applied in cellular imaging, drug and gene delivery, tissue engineering, and antibacterial therapies. It is a strong antibacterial alternative since it has severe toxic effects on bacteria, fungi, and other pathogens (Wu et al., 2015). The multiple applications of nanographene oxide, especially in the biomedical fields are increasing concerns about the potentially toxic effects of this substance on health and tissue cells. Therefore, the current study aimed to investigate the toxic effects of nanographene oxide on the testes of laboratory rats.
MATERIALS AND METHODS
A total of 48 male albino rats with an age range of 10-12 weeks and a mean weight of 170 g were examined for clinical health in the present study. The graphene oxide nanopowder was purchased from Sky Spring Nanomaterials, Inc., USA, in the form of black powder with 97% purity, 2-nm thickness, and an average diameter of 3-10 nm. Different concentrations of graphene oxide nanopowder were prepared by dissolving it in a normal saline solution. The animals were then divided into six groups and each group has a replicate (4 rats in each replicate). The first, second, third, fourth, and fifth groups were respectively treated with graphene oxide nanopowder at concentrations of 20, 30, 40, 50, and 60 mg/kg. The sixth group was considered as the control group. The solution was administered orally to the rats using a feeding tube about 2-3 inches in length to prevent wounding the animal. The volume of administration was 0.1 ml per
222
ISSN 2322-4568
A R
c n
c c
e i
p v
e ft
: :
— 2
6 4
M s
C a —y
O 2
2 C
3 2
0
1 —
G
HH
N A L
A R
T —
C L
E
each animal, for 30 days. At the end of the experiment, the blood samples were collected from the lateral tail vein of each rat into an EDTA tube and a silicon-coating tube. The samples of silicon coating tubes remained at room temperature for up to 30 minutes to enable clotting. The clot is removed by centrifugation at 2000 x g for 10 minutes and the resulting supernatant immediately transfer to a polypropylene tube using a Pasteur pipette. The serum levels of LH, FSH, and testosterone hormones were measured immediately after receiving the serum samples by commercial kits (Beijing Northern Biotechnology Research Institute, Beijing, China) in an automated chemistry analyzer (BS 200, Mindray, China). The light and electron microscope slides were prepared based on the method proposed by Luna (1968). The microscopical studies were carried out using a light microscope (Olympus, Japan) and a transmission electron microscope (TESCAN company, China) to see the histological and cellular changes resulting from the effect of this substance. The weights of the testes in the different groups were measured using a sensitive scale (PCE-LSZ 200C, PCE- Deutschland Gmbh & Co, Germany), and the lengths of these samples were also measured using the ruler.
Statistical analysis
The statistical analysis was carried out using one-way ANOVA followed by L.S.D. The probability level of < 0.05 was considered statistically significant.
RESULTS
Effects of graphene oxide on blood parameters
The results of the current study showed that there was a significant decrease in the average number of red blood cells in group 5 when compared with other groups (p < 0.05, Table 1). The results of the current study showed a significant decrease in the hemoglobin level of the group treated with a concentration of 60 mg/kg when compared to other groups (p < 0.05). Moreover, there was a non-significant increase in the average number of white blood cells when comparing the treated groups with concentrations of 20, 30, 40, and 50 mg/kg and the control group (p > 0.05). There was a significant increase in the rate of white blood cells when comparing treated rats with 60 mg/kg with other groups except for the fifth group (p < 0.05). The current study showed a significant increase in the mean number of plate lets when comparing the groups treated with 40, 50, and 60 mg/kg of graphene oxide with other groups (Table 1).
Effects of graphene oxide nanoparticles on weight and length of testes
Comparing the control group with the experimental groups showed a significant decrease in the mean testis weight of treated rats (p < 0.05, Table 2). There was a significant decrease in the mean testicular length of all treated rats, compared to the control rats (p < 0.05, Table 2).
Effects of graphene oxide on the levels of sex hormones
There was a significant decrease in the FSH level when comparing all the treated groups with the control group (p < 0.05, Table 3). The results of the current study also showed an insignificant difference in the rate of LH hormone when comparing all the treated groups with the control group (p > 0.05). However, there was a significant decrease in the rate of testosterone hormone in all experimental groups, compared with the control group (p < 0.05).
Table 1. Effects of different levels of nanographene oxide on some blood parameters of rats
Groups RBC Hb WBC Platelets
Treatment 1 (20 mg/kg) 8.11 ± 0.47a 14.12 ± 0.77a 5.06 ± 0.52a 437.20 ± 11.67a
Treatment 2 (30 mg/kg) 8.11 ± 0.46a 14.08 ± 0.29a 5.06 ± 1.18a 490.00 ± 18.95ab
Treatment 3 (40 mg/kg) 7.71 ± 0.45ab 13.46 ± 0.61a 5.28 ± 0.83a 597.60 ± 64.36e
Treatment 4 (50 mg/kg) 7.69 ± 0.26ab 12.64 ± 0.11ab 5.56 ± 1.11 ab 632.80 ± 44.48e
Treatment 5 (60 mg/kg) 6.82 ± 0.53c 10.76 ± 0.61c 6.46 ± 0.49b 640.60 ± 96.94e
Treatment 6 (Control) 8.71 ± 0.53a 14.26 ± 1.25a 4.40 ± 0.40a 368.80 ± 60.76a
The numbers in the table represent mean values ± standard deviation. RBC: Red blood cells, Hb: Hemoglobin, WBC: White blood cells. The different superscript letters in the same column indicate a significant difference at the probability level of p < 0.05.
Table 2. Effects of different levels of graphene oxide nanoparticles on the average weight and length of testes in rats
Groups_Weight of testis_Length of testis
Treatment 1 (20 mg/kg) 0.71 ± 0.04b 1.71 ± 0.09b
Treatment 2 (30 mg/kg) 0.69 ± 0.04c 1.63 ± 0.05c
Treatment 3 (40 mg/kg) 0.68 ± 0.03c 1.50 ± 0.12d
Treatment 4 (50 mg/kg) 0.67 ± 0.03cd 1.48 ± 0.07e
Treatment 5 (60 mg/kg) 0.63 ± 0.04 d 1.35 ± 0.05f
Treatment 6 (Control) 0.80 ± 0.04a 1.85 ± 0.05a
The numbers in the table represent mean values ± standard deviation. The different superscript letters in the same column indicate a significant difference at the probability level of p < 0.05.
223
Table 3. Effects of different levels of graphene oxide nanoparticles on sex hormones of rats
Groups FSH LH Testosterone
Treatment 1 (20 mg/kg) 0.52 ± 0.29b 1.15 ± 0.25 2.12 ± 0.04b
Treatment 2 (30 mg/kg) 0.42 ± 0.29b 1.14 ± 0.19 2.02 ± 0.29b
Treatment 3 (40 mg/kg) 0.30 ± 0.11b 1.06 ± 0.18 2.02 ± 0.16b
Treatment 4 (50 mg/kg) 0.25 ± 0.05bc 1.02 ± 0.13 1.86 ± 0.18b
Treatment 5 (60 mg/kg) 0.24 ± 0.05bc 1.01 ± 0.21 1.42 ± 0.20bc
Treatment 6 (Control) 0.92 ± 0.17a 1.22 ± 0.08 2.72 ± 0.57a
The numbers in the table represent mean values ± standard deviation. The different superscript letters in the same column indicate a significant difference at the probability level of p < 0.05.
Histological examinations
Histological examinations on the control rats showed the normal structure of the testis. As observed in the control group, the seminiferous tubules contain germ cells that represent the different stages of sperm formation. In addition to the mature sperms present in the lumen of the seminiferous tubule, the seminiferous tubules are separated from each other by connective tissue as they are the testicle surrounded by the capsule (Figure 1). The histological examinations of the testes in the rats of the group treated with a concentration of 20 mg/kg showed bleeding, while those treated with a concentration of 30 mg/kg indicated a decrease in the number of sperms and thickness of the tubular epithelium as well as the dissociation of the connective tissue between the seminiferous tubules (Figures 2 and 3). Necrosis of the tubular epithelium and severe bloody congestion as shown in figures 3 and 4. The histological examinations of the testes in the rats treated with a concentration of 40 mg/kg showed bleeding, a decrease in the number of sperms, dissociation of the connective tissue between the seminiferous tubules, and spacing of the seminiferous tubules (Figures 5 and 6). The tubular epithelium, lack of sperm, and the destruction of the seminal tubule wall, as well as the dissociation of the connective tissue between the seminiferous tubules, and a decrease in the thickness of the tubular epithelium, were seen in treated rats with a concentration of 50 mg/kg (Figures 7 and 8). Histological examinations of the testes in the experimental rats administered a concentration of 60 mg/kg showed a lack of connective tissue between the seminiferous tubules and necrosis of the tubular epithelium, as well as hematopoietic congestion, lack of sperm, degeneration of sperm-forming cells and edema formation (Figures 9 and 10).
Examinations by electron microscopy
Transmission electron microscopy images of the testis in the rats of the control group revealed a normal structure of spermatids, where the cell membrane, cytoplasm, vesicle, and nucleus could be observed (Figure 11). Moreover, transmission electron microscope images of the testis in the rats of the treated group at a concentration of 20 mg/kg of nanographene oxide showed the presence of a spermatid with two heads (Figure 12); however, the images of the testis in the rats treated with nanographene oxide at a concentration of 30 mg/kg indicated the destructions in the nucleus membrane (Figure 13). The nucleus membrane and the cell membrane of the testis were destroyed in the rats treated with nanographene oxide at concentrations of 40 and 50 mg/kg, respectively (Figures 14 and 15). The transmission electron microscopy images of the testis in the rats treated with nanographene oxide at a concentration of 60 mg/kg showed that the nucleus wall and the nucleus were dented in the sertoli cell (Figure 16).
Figure 1. Normal testicular tissue of rats showing tunica albuginea (A), seminiferous tubules (B), the connective tissue
between seminiferous tubules (C), H&E, 100X.
224
Figure 2. Testicular tissue of the rats treated with nanographene oxide at a concentration of 20 mg/kg indicates hemorrhagic bleed (A), progenitor sperm (B), the lumen of seminiferous tubule (C), spermatocytes in different stages
of formation (D), H&E, 400X
Figure 3. The testis tissue of the rats treated with nanographene oxide at a concentration of 30 mg/kg showis the dissociation of connective tissue between seminiferous tubules (A), necrosis of the tubular epithelium (B), decreased
sperm count (C), H&E, 400X
Figure 4. The testis tissue of the rats treated with nanographene oxide at a concentration of 30 mg/kg shows severe
blood congestion (A) tubular epithelium necrosis (B) H&E, 400X —225
To cite this paper: Abd-Alsahib EF, and Faris SA (2023). Toxic Effects of Nanographene Oxide on Testes of Rats. World Vet. J., 13 (1): 222-233. DOI: https://dx.doi.org/10.54203/scil.2023.wvj24
Figure 5. A cross-section of the testicular tissue of the rats treated with nanographene oxide at a concentration of 40 mg/kg indicates hemorrhage (A), decrease in sperm count (B) H&E, 400X
Figure 6. Testicular tissue of the rats treated with nanographene oxide at a concentration of 40 mg/kg reveals the dissociation of connective tissue between the seminiferous tubules (A), decrease in the number of sperm (B), spacing
between the seminiferous tubules (C) H&E, 400X
Figure 7. The testis tissue of the rats treated with nanographene oxide at a concentration of 50 mg/kg indicates the rupture of the wall of the seminiferous tubule (A), the dissociation of the connective tissue between the seminiferous tubules (B), Necrosis of the tubular epithelium (C), H&E, 400X
Figure 8. The testis tissue of the rats treated with nanographene oxide at a concentration of 50 mg/kg presents hemocongestion (A), lack of sperm (B), decrease in the tubular epithelium (C), H&E, 400X
Figure 9. The testis tissue of the rats treated with nanographene oxide at a concentration of 60 mg/kg demonstrates a lack of connective tissue between the seminiferous tubules (A) necrosis of the tubular epithelium (B), H&E, 400X
Figure 10. The testis tissue of the rats treated with nanographene oxide at a concentration of 60 mg/kg indicates blood congestion (A), necrosis of the tubular epithelium (B), lack of sperm (C), degeneration of sperm-forming cells (D), occurrence of edema (E),
H&E, 400X
Figure 11. The testis tissue in control group rats showing the structure of the spermatid (A), cell membrane (B), cytoplasm (C), vesicle (D), centrioles (E), nucleus, transmission electron microscopy
Figure 12. The testicular tissue of the rats treated with nanographene oxide at a concentration of 20 mg/kg indicates the structure of a double-headed spermatid (A), nucleus (B), cytoplasm (C), centriole (D), cell membrane, transmission
electron microscopy
Figure 13. The testicular tissue of the treated rats with nanographene oxide at a concentration of 30 mg/kg. A nucleophilic breakdown in the cytoplasm (A, B), transmission electron microscopy
228
Figure 14. The testicular tissue of rats treated with nanographene oxide at a concentration of 40 mg/kg shows the breakdown of nucleus membrane (A), transmission electron microscopy
Figure 15. The testicular tissue of rats treated with nanographene oxide at a concentration of 50 mg/kg indicates cell membrane breakdown (A), nucleus (B), cytoplasm (C), transmission electron microscopy
Figure 16. The testicular tissue of rats treated with nanographene oxide at a concentration of 60 mg/kg demonstrates buckling membrane of the nucleus (A), Golgi (B), Golgi apparatus (C), cytoplasm (D), nucleus (E), mitochondria in
sertoli cell, transmission electron microscopy
DISCUSSION
Effect of graphene oxide on the blood
The current study indicated a decrease in the number of red blood cells in all treated rats, especially in rats treated with 60 mg/kg of graphene oxide. The oxidative stress or pathological damage caused by the sharp edges of this material indicated that graphene oxide causes toxicity to the size and oxygen content of red blood cells leading to the dissolution of red blood cells (Liao et al. 2011). Moreover, this result agrees with a study of Puzyr et al. (2007), which showed the presence of toxic effects on blood parameters after intravenous injection, as graphene oxide interacts with the outer membrane of red blood cells through electrostatic interactions. This causes a disturbance in the polarity or permeability of the membrane and promotes hemolysis. These results are not in line with the obtained result of Qu et al. (2013), revealing that carbon nanoparticles do not cause any side effects to blood cells because they do not exert any toxic effects on blood cells. The results of the current study also revealed a decrease in the percentage of hemoglobin in the groups treated with nanographene oxide, compared to the control group. Hemolysis can be caused by the sharp edges of these nanomaterials that damage cell membranes, which was reported by Feng et al. (2015). The morphological changes, aggregation, and hemolytic effects on red blood cells when treated with graphene oxide are also indicated by Sasidharan et al. (2012). The reason behind the aggregation and hemolysis is the interaction between the hydrophobic surface of graphene and the lipid bilayer of the red blood cell membrane, or other interactions, such as hydroxyl and carboxylic groups in graphene oxide. These results also agree with those obtained by Stone et al. (2017), where a significant decrease was found in the hemoglobin percentage of groups treated with graphene oxide at concentrations of 80, 120, and 200 mg/kg, compared to the control group. In contrast, Escudero et al. (2019) reported that treating rats with graphene and graphene oxide could lead to smaller-sized red blood cells containing a high percentage of hemoglobin, justifying the reason for the high hemoglobin to compensate for the small size of red cells. The results of the current study also showed an increase in the numbers of white blood cells and platelets in the groups treated with graphene oxide, compared to the control group. There was an increase in the number of white blood cells although it decreased over time meaning that this effect was temporary. These results are consistent with a study by Vuppaladadium et al. (2020), where it was found that the number of white blood cells increased in mice treated with graphene oxide. This enhancement can be related to the foreign body, as it occurs after consuming the drug or graphene oxide. On the contrary, Rathnam et al. (2020) found that the hemoglobin content and the number of red blood cells were close to the control group. They also reported a slight increase in leukocytes, perhaps due to the response to nanomaterials during treatment. Pinto et al. (2013) and Zainab et al. (2021) also found that graphene-based nanomaterials were compatible with blood and did not cause hemolysis, platelet activation, changes in coagulation, or abnormalities in blood parameters. However, Lindstrom et al. (2015) reported that the number of white blood cells did not change in the animals treated with reduced graphene oxide.
Effect of graphene oxide on the testes
The results of the current study showed a decrease in the rate of testes' weight in the groups treated with graphene oxide. Compared with the control group, the histological examinations of the testes in rats treated with graphene oxide showed a group of changes represented by hemorrhage, a decrease in the number of sperms, a decrease in the thickness of the tubular epithelium, in addition to the dissociation of the connective tissue between the seminiferous tubules, necrosis of the tubular epithelium and blood congestion. The reason for the decrease in the weight of the testicles in the treated groups may be attributed to the lack of sperm and the suspension of the cell cycle, which resulted from a defect in the process of sperm formation in addition to the necrosis of the tubular epithelium leading to the loss of spermatozoa. These results were supported by Nirmal et al. (2017), indicating a decrease in the number of sperm in that rats exposed to graphene oxide nanoparticles for 15 and 30 days.
Histopathological changes in the testes, such as necrosis and a decrease in the thickness of the tubular epithelium, may be due to oxidative stress, as oxidative stress leads to the oxidation of cell membrane lipids, and consequently cell death. Adenosine triphosphate (ATP) is rapidly removed from the sperm, causing axonal damage and increasing sperm morphological abnormalities. These results were confirmed in a study by Mathur and Dcruz (2011), where there was a decrease in the number of sperms, their movement, and deformation due to a defect in the process of sperm formation as a result of the imbalance between oxidizing factors and antioxidant factors. Similarly, Cherian et al. (2014) found that oxidative stress and reactive oxygen species decreased cell proliferation, reduced steroid hormones, loss of germ cells, and cell death in the germinal epithelium. These results were also consistent with a study by Thakur et al. (2014), where nanomaterials cause the irregular appearance of the testis with atrophy of seminiferous tubules, loss of sperm-generating germ cells, necrosis of germ cells, and decrease or disappearance of sperms. In the same line, Hafsan et al. (2022), Huldani et al. (2022), and Li et al. (2016) found that Zno nanoparticles could cause reactive oxygen species (ROS) generation and DNA damage to germ cells and downregulation of the expression of proteins in sertoli cells, which may cause damage to the blood barrier in the testis. However, Liang et al. (2015) reported that the level of testosterone
230
hormone in the serum did not change as it was shown that these nanomaterials did not stimulate the tissue damage of the testes and epididymis. These results do not agree with a study by Nirmal et al. (2017), indicating the concentrations of sex hormones in the serum of rats treated with graphene oxide did not change, compared to the control group after intravenous injection and the sexual behavior in male rats as reproductive structures were normal.
Results of transmission electron microscope
The findings of the transmission electron microscope showed changes at the cellular level of the testis, represented by the appearance of bi-headed and smashed-headed spermatids, in addition to the presence of sperms with broken membranes and other shrunken flagella walls in sertoli cells. This may be attributed to the ability of graphene oxide sheets to penetrate the cell membrane due to their nanoscale, causing damage to cell components through several mechanisms, including direct interaction with large biomolecules present in the cell or through oxidative stress that causes cell damage. The interaction of graphene oxide with large biological molecules, such as proteins, can result from functional groups with electrical charges on the surface of graphene oxide. Consequently, abnormalities occur in the intracellular signaling pathways that regulate cell growth, proliferation, differentiation, or survival. Similarly, Shi et al. (2012) reported that graphene oxide can weaken membrane proteins that act as carriers of nutrients or essential biomolecules, thus leading to a decrease in metabolic activity in cells treated with graphene derivatives. The mechanisms of action of graphene nanomaterials on living organisms include oxidative stress, inflammatory response, apoptosis, autophagy, and necrosis (Wu et al., 2021). Another study showed that treatment of some in vitro cultured cells, such as THP-1 and BEAS-2B cells, with graphene oxide, causes cytotoxicity by oxidizing lipids in the membrane and causing damage to the cell membrane (Li et al., 2018; Ansari et al., 2022). Another study showed that small parts of nanosheets of graphene derivatives could enter the nucleus, interact directly with DNA, and cause damage (Xu et al., 2018; Abolhasani Zadeh et al., 2022). In contrast to these results, some researchers stated that the graphene family of nanomaterials does not negatively affect the nucleus and DNA (Jarosz et al., 2016; Bokov et al., 2022).
CONCLUSION
It can be concluded that the nanographene oxide at 20-60 mg/kg concentrations is a hazardous material for testis heath in rats which can have dangerous effects on spermatogenesis, testosterone, and FSH levels and can destroy the testicular structure and impairment the testicular function in rats. The examined results of the transmission electron microscope confirmed the negative effects of this material in investigated dosages. The authors of the present study suggest that lower doses of nanographene oxide will consider for future histopathological studies in laboratory rats.
DECLARATIONS
Authors' contributions
The authors contributed equally to the study design, collecting the samples, statistical analysis, writing the first draft of the manuscript, and following the revisions of the last draft of the article for submission to the journal.
Conflicts of interests
The authors of the present study have no conflicts of interest to declare. Ethical considerations
Ethical issues (including plagiarism, consent to publish, misconduct, data fabrication and/or falsification, double publication and/or submission, and redundancy) have been checked by the authors.
Funding
This article is funded by the College of Education for Pure Sciences, University of Thi-Qar, Iraq. Availability of data and materials
The authors declare that they can prepare datasets for this study upon reasonable request.
REFERENCES
Ansari MJ, Jasim SA, Taban TZ, Bokov DO, Shalaby MN, Al-Gazally ME, Kzar HH, Qasim MT, Mustafa YF, and Khatami M (2022). Anticancer drug-loading capacity of green synthesized porous magnetic Iron nanocarrier and cytotoxic effects against human cancer cell line. Journal of Cluster Science, 34: 467-477. DOI: https://www.doi.org/10.1007/s10876-022-02235-4
231
Cherian RS, Sreejith R, Syama S, Sruthi S, Gayathri V, and Maekawa T (2014). Evaluation of toxicity of Maura reduced graphene oxide using in vitro systems. Journal of Nanomedicine & Nanotechnology, 5 (3): 1-9. DOI: https://www. doi. org/10.4172/2157-7439.1000200
Dhiman L and Dhamiji A (2014). Multifaceted graphene: Novelity in electronics. International Journal of Advanced Research in Electrical, Electronics and Instrumentation Engineering, 3(9): 11807-11811. Available at: https://www.iiareeie.com/upload/2014/september/19 Multifaceted.pdf
Escudero ML, Llorente I, Perez-Maceda BT, San S, Sanchez-Lopez L, Lozano RM, Aguado-Henche S, Clemente C, Alobera-Gracia MA, and Garcia-Alonso MC (2019). Electrochemically reduced graphene oxide on CoCr biomedical alloy: characterization, macrophage biocompatibility and hemocompatibility in rats with graphene and graphene oxide. Materials Science & Engineering: C, (109): 110552. DOI: https://doi.org/10.1016/j.msec.2019.110522
Feng R, Yu Y, Shen C, Jiao Y, and Zhou C (2015). Impact of graphene oxide on the structure and function of important multiple blood components by a dose-dependent pattern. Journal of Biomedical Materiales Research, Part A, 103(6): 2006-2014. DOI: https://www.doi.org/10.1002/ibm.a.35341~
Bokov DO, Abduladheem TJ, Alsultany FH, Mahmoud MZ, Suksatan W, Chupradit S, Qasim MT, and Kheirollahi Nezhad PD (2022). Ir-decorated gallium nitride nanotubes as a chemical sensor for recognition of mesalamine drug: A DFT study. Molecular Simulation, 48 (5): 438-447. DOI: https://www.doi.org/10.1080/08927022.2021.2025234
Jarosz A, Skoda M, Dudek I, and Szukiewicz D (2016). Oxidative stress and mitochondrial activation as the main mechanisms underlying graphene toxicity against human cancer cells. Oxidative Medicine and Cellular Longevty, 2016: 5851035. DOI: https://www.doi.org/10.1155/2016/5851035
Hafsan H, Bokov D, Abdelbasset WK, Kadhim MM, Suksatan W, Majdi HS et al. (2022). Dietary Dracocephalum kotschyi essential oil improved growth, haematology, immunity and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). DOI: https://www.doi.org/10.1111/are. 15829
Huldani H, Jasim SA, Bokov DO, Abdelbasset WK, Shalaby MN, Thangavelu L, Margiana R, and Qasim MT (2022). Application of extracellular vesicles derived from mesenchymal stem cells as potential therapeutic tools in autoimmune and rheumatic diseases. International Immunopharmacology, 106: 108634. DOI: https://www.doi.org/10.1016/i.intimp.2022.108634
Li Y, Wang Y, Tu L, Chen D, Luo Z, Liu D, Miao Z, Feng G, Qing L, and Wang S (2016). Sub-acute toxicity study of graphene oxide in the Sprague Dawley Rat. International Journal of Environmental Research and Public Health, 13(11): 1149. DOI: https://www.doi.org/10.3390/iierph13111149
Li R, Guiney LM, Chang CH, Mansukhani ND, Wang X, Liao YP, Jiang W, Sun B, and Hersam MC (2018). Surface oxidation of graphene oxide determines membrane damage, lipid peroxidation, and cytotoxicity in macrophage in a pulmonary toxicity model. American Chemical Society Nano, 12(2):1390-1402. DOI: https://www. doi. org/10.1021 /acsnano.7b07737
Liao KH, Lin YS, Macosko CW, and Haynes CL (2011). Cytotoxicity of graphene oxide and graphene in human erythrocytes and skin fibroblasts. American Chemical Society Applied Materials and Interfaces, 3(7): 2607-2615. DOI: https://www.doi.org/10.1021/am200428v
Liang S, Xu S, Zhang D, He J, and Chu M (2015). Reproductive toxicity of nanoscale graphene oxide in male mice. Nanotoxicology, 9(1): 92-105. DOI: https://www.doi.org/10.3109/17435390.2014.893380
Lindstrom NM, Moore DM, Zimmerman K, and Smith SA (2015). Hematologic assessment in pet rats, mice, hamters, and gerbils: blood sample collection and blood cell identification. Veterinary Clinics of North America: Exotic Animal Practice, 18(1): 21-32. Available at: https://www.cabdirect.org/cabdirect/abstract/20143408666
Luna LG (1968). Manual of histological staining methods of armed forces institute of pathology, 3rd Edition. Mc Graw-Hill Book., Newyork. London. (9): 1-74.
Mathur PP and D'Cruz SC (2011). The effect of environmental contaminants on testicular function. Asian Journal of Andrology, 13(4): 585-591. DOI: https://www.doi.org/10.1038/aja.2011.40. Epub 2011 Jun 27
Markovic ZM, Harhaji-Trajkovic LM, Todorovic-Markovic BM, Kepic DP, Arsikin KM, Jovanovic SP, Pantovic AC, Drami Canin MD, and Trajkovic VS (2011). In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. Biomaterials, 32(4): 1121-1129. DOI: https://www.doi.org/10.1016/j.biomaterials.2010.10.030
Nirmal NK, Awasthi KK, and Jhob PJ (2017). Effects of nanographene 0xide on testis, epididymis and fertility of wistar rats. Basic & Clinival Pharmacology & Toxicology, 121(3): 202-210. DOI: https://www.doi.org/10.1111/bcpt.12782
Priyadarsini S, Mohanty S, Mukherjee S, Basu S, and Mishra M (2018). Graphene and grapheme oxide as nanomaterials for medicine and biology application. Journal of Nanostructure in Chemistry, 8: 123-137. Available at: https://www.sid.ir/paper/333347/en
Pinto AM, Goncalves IC, and Magalhes FD (2013). Graphene-based materials biocompatibility: A review. Colloids and Surfaces B: Biointerfaces, 111: 188-202. DOI: https://www.doi.org/10.1016/j.colsurfb.2013.05.022
Puzyr AP, Baron AV, Purtov KV, Bortnikov EV, Skobelev NN, Mogilnaya OA, and Bondar VS (2007). Nanodiamonds with novel properties: A biological study. Diamond and Related Materials, 16(12): 2124-2128. DOI: https://www.doi.org/10.1016/j.diamond.2007.07.025
Qu G, Wang X, Liu Q, Liu R, Yin N, Ma J, Chen L, He J, Liu S, and Jiang G (2013). The ex vivo and in vivo biological performances of graphene oxide and the impact of surfactant on graphene oxide's biocompatibility. Journal of Environmental Sciences, 25(5): 873-881. DOI: https://www.doi.org/10.1016/S1001-0742(12)60252-6
Rathnam VS, Agarwal T, Kulanthaivel S (2020). Silanization improves biocompatibility of graphene oxide. Materials Science and Engineering: C, 110: 110647. DOI: https://www.doi. org/10.1016/j .msec .2020.110647
Sasidharan A, Panchakar LS, Sadanandan AR, Ashokan A, Chandran P, Girish CM, Menon D, Nair SV, Rao CN, and Koyakutty M (2012). Hemocompatibility and macrophage response of pristine and functionalized graphene, Small, 8(8): 1251-1263. DOI: https://www.doi.org/10.1002/smll.201102393
232
Shi XT, Chang HX, Chen S, Lai C, Khademhossrini A, and Wu HK (2012). Regulating cellular behavior on few-layer reduced graphene oxide films with well-controlled reduction states. Advanced Functional Materials, 22(4): 751-759. DOI: https://www.doi.org/10.1002/adfm.201102305
Stone V, Miller MR, Clift MJ, Elder A, Mills NL, Maller P, Schins RP, Vogel U, Kreyling WG, Alstrup Jensen K et al. (2017). Nanomaterials versus ambient ultrafine particles: An opportunity to exchange toxicology knowledge. Environmental Health Perspectives, 125(10): 106002. DOI: https://doi.org/10.1289/EHP424
Vuppaladadium SSR, Agarwal T, Kulanthaivel S, Mohanty B, Barik CS, Maiti TK, and Banerjee I (2020). Silanization improves biocompatibility of graphene oxide. Materials Science and Engineering: C, 110: 110647. DOI: https://www.doi.org/10.1016/j.msec.2020.110647
Wu L, Zeng L, and Jiang X (2015). Revealing the nature of interaction between graphene oxide and lipid membrane by surface-enhanced infrared absorption spectroscopy. American Chemical Society, 137(32): 10052-10055. DOI: https://www.doi.org/10.1021/iacs.5b03803
Wu K, Zhou Q, and Ouyang S (2021). Review direct and indirect genotoxicity of grapheme family nanomaterials on DNA-A review. Nanomaterials, 11(11): 2889. DOI: https://www.doi.org/10.3390/nano11112889
Abolhasani Zadeh F, Bokov DO, Salahdin OD, Abdelbasset WK, Jawad MA, Kadhim MM, Qasim MT, Kzar HH, Al-Gazally ME, Mustafa YF et al. (2022). Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF-7 cancer cells. Rendiconti Lincei Scienze Fisiche e Naturali, 33:441-447. Available at: https://link.springer.com/article/10.1007/s12210-022-01064-x
Thakur M, Gupta H, and Singh D (2014). Histopathological and ultrastructure effects of nanoparticles on rat testis following 90 days (chronic study) of repeated oral administration. Journal of Nanobiotechnology, 12: Article number 42. DOI: https://doi.org/10.1186/s12951-014-0042-8
Zainab I, Mohammed M, and Qasim T (2021). Hormonal profile of men during infertility. Biochemical and Cellular Archives, 21(Supplement 1): 2895-2898. Available at:
https://connectiournals.com/toc2.php?abstract=3404600H 2895A.pdf&&bookmark=CJ-033216&&issue id=Supp-01&&yaer=2021
233
