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11THE TOXIC HEPATITIS ANDDYNAMICS ANTIHEPATIC ANTIBODIES IN WHEY OF BLOOD AND MILK FEMALE RATS WITH DURING BRAEST FEEDING
KhasanovB.
Bukhara State medical Institute, Department of histology, Cytology and embryology,
Bukhara, Uzbekistan)
Abstract
Presence of antihepatic antibodies in whey of blood and at rats with toxic hepatitis are investigated during breast-feeding. Presence autoantibodies is established in whey of blood and scant fews in milk of rats during, hence, of mother at a toxic hepatitis are not the pathological agent for the baby during breast-feeding. Keywords: toxic hepatitis, blood, milk, antihepatic antibodies, rats, lactation.
The high incidence of viral hepatitis, including in women of childbearing age, does not remove the relevance of this infection at the present time. The possibility of transmission of the hepatitis virus from female carriers of HbsAg to children born, the development of HBsAg persistence in some children, and the formation of primary chronic hepatitis is not in doubt [6]. However, the significance of autoimmune processes that occur in the mother's body during hepatitis, in the dynamics of lactation and their impact on the development of offspring during breastfeeding are still poorly understood. While there is a significant number of studies that indicate that hepatitis in the mother leads to a slowdown in the formation of immune components of the small intestine, as well as the organs of the immune system of the offspring in early postnatal ontogenesis [2,4,8]. However, the question of the causes and mechanisms that lead to these changes remains poorly understood.
The aim of our study was to study the titer of anti-hepatocyte antibodies in the blood serum and milk of female rats with toxic hepatitis in the dynamics of lactation.
Material and methods of research.
For the experiment, 3-month-old sexually Mature white mongrel female rats (72) weighing 120-140 grams were used. The animals were kept on a normal laboratory diet and were quarantined for two weeks prior to the experiment. We used chronic heliotrine intoxication as a model of hepatitis [1]. After the quarantine period, the females of the experimental group (O) were administered heliotrin at a dose of 0.05 mg/gram of body weight per 0.5 ml of saline solution subcutane-ously weekly for 6 weeks, the animals of the control group (K) were administered only saline. 10 days after the last injection, the males were hooked to the females. For the study, females of the experimental (40) and control groups (32) were selected for 1, 3, 7, 15, 21 and 30 days of lactation. Definition antiseptically antibody TPPA was performed according to the method of Boyden. Serological reactions were performed using blood serum (0.5 ml) and milk samples (0.2 ml) taken from female rats during the above study period. The sheep's red blood cells were prepared according to the generally accepted method. As an antigen, a liver sample of an intact female rat was used in the amount of 2.0 g, which was prepared by water-chloroform extraction using the method of T. A. Alekseeva [6]. The content of the antigen was controlled by the amount of protein, which was brought to 1%. For more accurate calculation and ease of comparison, the results were expressed in log2[5].The obtained data were processed by
Fischer-Student, and differences satisfying P<0.05 were considered reliable.
Results and discussion
As a result of our study, a certain dynamics of changes in the titer of anti-hepatocyte antibodies in the blood and milk of rats with toxic hepatitis in the dynamics of lactation was established. In the blood serum of females of the experimental group immediately after birth, autoantibodies reaching a titer of 1:128 are detected, this trend persists until 3 days of lactation, while in the control group of animals, the titer of autoantibod-ies is 1:8 and 1:4 on 1 and 3 days after birth, respectively. In the subsequent lactation periods seropositive serums were diluted 1: 64 until the end of the lactation period. On the contrary, in the control group, antibodies are detected in females only up to 7 days of breastfeeding, not exceeding the titer 1:4, in subsequent periods of lactation, only traces were observed.
In contrast to the blood serum in serological study of milk samples of females of the experimental group on 1 day after delivery, autoantibodies were determined in a 1:8 dilution. in subsequent periods, anti-hepatic antibodies were not always detected even in a 1:4 dilution . In the control group, the indicators of serological studies of milk during breastfeeding did not show significant differences from the experimental group.
For more accurate studies and the possibility of comparing changes in the titer of anti-hepatocyte antibodies in blood serum and milk of female rats in the dynamics of lactation, the results of serological studies were expressed in log2 (see Table). According to these data, in toxic heliotrine hepatitis, the appearance of autoantibodies against the liver is characteristic only for blood serum, which are present until the end of the breastfeeding period. While in milk samples obtained from females with toxic hepatitis, the titer of autoanti-bodies did not differ significantly from those in the control group of females.
It is known that chronic heliotrine intoxication leads to the emergence of toxic hepatitis, which generally tends to progress, i.e. it is a model of aggressive chronic hepatitis [1]. One of the main mechanisms for activating this process is the formation of anti-hepatic antibodies [5]. The results of our study once again showed that heliotrin intoxication of female rats before pregnancy is the trigger of an autoallergic process that progresses over time. Along with this, relatively low titers of anti-hepatic antibodies in the blood serum, apparently, are associated with changes occurring in the mother's body in the dynamics of pregnancy and lactation, stimulating regenerative processes. Almost twice the excess of the autoantibodies titer immediately after
birth, relative to the further period of lactation (1: 128 on 1 day and 1: 64 in subsequent periods of lactation),
The presence of a small amount of antibodies in milk during lactation indicates that the cause of dys-trophic changes in the liver of rats born of an intact rat, when they are fed by a female with heliotrine hepatitis [3], is not autoantibodies, but possibly hepatotoxins formed in the mother's body, due to liver disorders [8, 9, 10].
Thus, our results allow us to conclude that in the blood of female rats with toxic hepatitis after birth and in the dynamics of lactation, anti-hepatic antibodies are determined, but during breastfeeding, they are transmitted to the ratlets through milk in small quantities and are not the cause of lagging in the formation of the digestive and immune systems of the offspring.
References
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Table
Changes in the activity of anti-hepatic antibodies in the blood and milk of female rats with chronic heliotrine in_toxication in the dynamics of lactation (data expressed in log2, M±m)_
Research material Gr.W-x Lactation period (in days)
1 3 7 14 21
Blood К 1,68± 0,158 0,75± 0,161 0,68± 0,149 0,48± 0,142 0,33± 0,104
О 6,03± 0,089* 5,45± 0,127* 5,10± 0,114* 4,90± 0,120* 4,73± 0,114*
Milk К 1,60± 0,206 1,48± 0,089 1,30± 0,082 0,83± 0,196 0,78± 0,164
О 3,75± 0,133 3,45± 0,158 3,43± 0,212 2,00± 0,199 1,95± 0,225
Note: * - differences are significant relative to control at P<0.05
КАРДИОЦЕРЕБРАЛЬНЫЕ ВЗАИМОСВЯЗИ И ДЛИНА ТЕЛОМЕР НА РАЗНЫХ СТАДИЯХ ЦЕРЕБРАЛЬНОГО АТЕРОСКЛЕРОЗА: МИФ ИЛИ ФАКТ?
Черская М.С.
ГУ «Институт эндокринологии и обмена веществ имени В.П. Комисаренко НАМН Украины»,
зав. консультативно-диагностическим отделением, канд. мед. наук
Кондратюк В.Е.
Национальный медицинский университет имени А.А. Богомольца, зав. каф. пропедевтики внутренних болезней № 2, д-р мед. наук, профессор
CARDIOCEREBRAL RELATIONSHIPS AND TELOMERE LENGTH AT DIFFERENT STAGES OF CEREBRAL ATHEROSCLEROSIS: MYTH OR FACT?
Cherska M.
State Institution "VP Komisarenko Institute of Endocrinology and Metabolism of the
National Academy of Medical Sciences of Ukraine ", Head of the Consultative and Diagnostic Department, PhD.
Kondratiuk V.
A.A. Bogomolets National Medical University, Head of the Department of Internal Medicine Propedeutics No. 2, Professor
