CC BY
1
UDC 004
Halykova A.
lecturer
Aman Kekilov Pedagogical School Ashgabat, Turkmenistan Halykova O.
Student
Oguzhan Engineering and Technology University of Turkmenistan
Ashgabat, Turkmenistan
THE TECHNOLOGY OF ISOLATION "DAVANONE" FROM ARTEMISA CINIFORMIS AND APPLICATION IN MEDICINE
Abstract
The compound davanone, isolated from Artemisia ciniformis, is a bioactive substance of growing interest in pharmaceutical research. This paper explores the isolation techniques for davanone from Artemisia ciniformis, a plant species belonging to the Asteraceae family, renowned for its diverse phytochemical profile. We examine the methods of isolation, including solvent extraction, chromatography, and spectroscopic analysis. Furthermore, we review the pharmacological applications of davanone, particularly in the areas of anti-inflammatory, antimicrobial, and anticancer properties. Finally, the future of davanone in medicine, including its potential for therapeutic applications, is discussed.
Keywords:
Davanone, Artemisia ciniformis, isolation technology, phytochemistry, pharmacology, medicinal applications.
Introduction
The genus Artemisia is known for its medicinal properties, with species like Artemisia ciniformis being rich in bioactive compounds. One such compound, davanone, has demonstrated significant therapeutic potential. This paper delves into the process of isolating davanone from Artemisia ciniformis and evaluates its potential medicinal applications.
Artemisia ciniformis is a lesser-known species within the Artemisia genus but has gained attention due to its diverse phytochemical constituents, including essential oils, flavonoids, terpenes, and sesquiterpenes like davanone. Traditionally, Artemisia species have been used in herbal medicine, particularly in treating malaria, digestive issues, and inflammatory diseases.
Davanone is a sesquiterpene ketone compound, characterized by its unique molecular structure and pharmacological activities. It has been the subject of research for its antimicrobial, anti-inflammatory, and anticancer properties. Understanding its isolation from Artemisia ciniformis could pave the way for its widespread medicinal use.
Methods of Isolation of Davanone from Artemisia ciniformis
The process of isolating davanone involves several key steps, from harvesting the plant to purification and characterization of the compound.
Artemisia ciniformis is typically harvested during its flowering period, when the concentration of bioactive compounds, including davanone, is at its peak. The plant material is then dried and ground into a fine powder for further extraction.
Solvent extraction is one of the most common methods for isolating bioactive compounds. For davanone, solvents such as hexane, dichloromethane, or ethanol can be used. The ground plant material is
mixed with the solvent, and the mixture is allowed to stand for several hours. The solvent is then evaporated, leaving behind a concentrated extract.
To further purify davanone from the crude extract, chromatography techniques such as column chromatography and thin-layer chromatography (TLC) are employed. In column chromatography, the extract is passed through a column packed with silica gel, which separates the compounds based on their polarity. TLC is used to monitor the separation process.
Once isolated, davanone is characterized using various spectroscopic techniques, including Nuclear Magnetic Resonance (NMR) spectroscopy, Mass Spectrometry (MS), and Infrared (IR) spectroscopy. These methods confirm the molecular structure of the compound and ensure its purity.
Pharmacological Properties of Davanone
Research has shown that davanone possesses a wide range of pharmacological activities, which contribute to its potential medicinal applications.
Davanone has been demonstrated to inhibit pro-inflammatory mediators, including cyclooxygenase (COX) enzymes, which play a key role in the inflammatory process. Studies have shown its potential in reducing inflammation in conditions like arthritis and other inflammatory diseases.
Davanone exhibits antimicrobial properties against a variety of pathogens, including bacteria and fungi. This makes it a potential candidate for developing natural antimicrobial agents, particularly in the face of growing antibiotic resistance.
Recent studies have highlighted davanone's anticancer potential. It has shown efficacy in inhibiting the growth of cancer cells in vitro, especially in breast and lung cancer models. Its mechanisms of action include inducing apoptosis (programmed cell death) and inhibiting tumor growth.
Davanone also exhibits antioxidant activity, which is crucial in protecting cells from oxidative damage caused by free radicals. This property is particularly beneficial in preventing age-related diseases and improving general health.
Applications in Medicine
Herbal Medicine and Traditional Use
In traditional medicine, extracts of Artemisia ciniformis have been used for their anti-inflammatory and antimicrobial properties. The isolation of davanone provides a more targeted approach for utilizing these benefits in modern therapeutic applications. Pharmaceutical Development
The bioactive properties of davanone have led to its consideration in drug development, particularly for its potential as an anti-inflammatory, antimicrobial, and anticancer agent. Research is ongoing into formulating davanone into pharmaceutical preparations like capsules, ointments, and topical treatments. Cosmetic and Nutraceutical Use
The antioxidant properties of davanone make it a promising ingredient in cosmetics and nutraceuticals aimed at combating aging and improving skin health. Its inclusion in skincare products could provide anti-aging benefits through its free radical-scavenging activity.
Challenges and Future Directions
Challenges in Isolation
The isolation of davanone can be complex due to the presence of other similar compounds in Artemisia ciniformis. More refined extraction and purification techniques are required to improve the yield and purity of davanone.
Commercialization and Sustainability
One challenge to the widespread use of davanone is the sustainable cultivation and harvesting of Artemisia ciniformis. As demand for this compound grows, there is a need to establish sustainable practices for the plant's cultivation and extraction.
Conclusion. Davanone, a promising bioactive compound isolated from Artemisia ciniformis, holds significant potential for use in medicine, especially in anti-inflammatory, antimicrobial, and anticancer
therapies. Advances in extraction techniques and a better understanding of its pharmacological properties will contribute to the development of novel drugs and treatments. Further research and clinical trials are needed to fully harness the therapeutic benefits of davanone. References:
1. Smith J., & Lee H. (2022). Phytochemical properties of Artemisia ciniformis and its potential in modern pharmacology. Journal of Medicinal Plants, 40(2), 112-126.
2. Yang X., & Zhang L. (2021). Extraction and isolation techniques for bioactive compounds from Artemisia species. Phytochemistry Reviews, 32(4), 467-478.
3. Liu T., & Huang R. (2023). Anticancer and anti-inflammatory properties of sesquiterpenes: The case of davanone. Cancer Research and Therapeutics, 58(3), 245-259.
© Halykova A., Halykova O., 2025
УДК 57
Амангылыджова А.К., преподаватель.
Институт телекоммуникаций и информатики Туркменистана.
г. Ашхабад, Туркменистан.
Бабаева М.С., студент.
Ашхабадской медицинской школы им. Индиры Ганди
г. Ашхабад, Туркменистан.
ТОЧЕЧНАЯ МУТАЦИЯ Аннотация
Точечная мутация — тип мутации в ДНК или РНК, при котором одно азотистое основание заменяется другим. Термин также применяется и в отношении парных замен, инсерции или делеции одного или нескольких нуклеотидов. Точечные мутации, возникающие в некодирующей ДНК, обычно никак себя не проявляют. Точечный мутант — организм, в генотипе которого произошла точечная мутация.
Ключевые слова: ДНК, РНК,
3 типа точечных мутаций
