CC BY
8B-I-7
BIOMEDICAL PHOTONICS
The study of laser-assisted skin optical clearing in vivo
E.A. Genina1-2'. V.D. Genin12, A.B. Bucharskaya13, N.A. Navolokin13, G.S. Terentyuk13,
N.G. Khlebtsov4, V.V. Tuchin125
1 - Saratov State University, 83 Astrakhanskaya str. Saratov, 410012, Russia 2 - Tomsk State University, 36 Lenin's av. Tomsk, 634050, Russia 3 - Saratov State Medical University, 112 Bolshaya Kazachia str., Saratov, 410012, Russia 4 - Institute of Biochemistry and Physiology of Plants and Microorganisms, 13 Prospekt Entuziastov, Saratov, 410049, Russia 5 - Institute of Precision Mechanics and Control RAS, 24 Rabochaya str., Saratov, 410028, Russia
*eagenina@yandex.ru
Photothermal therapy (PTT), which refers to treatment methods based on photothermal conversion under the action of light radiation, is attracting increasing attention due to its growing potential in oncology [1]. Due to the serious side effects of traditional tumor treatment strategies such as chemotherapy and radiation therapy, PTT may eventually become an alternative. To increase the selectivity of tumor hyperthermia, plasmon resonance nanoparticles can be used, which have a local surface plasmon resonance in a certain spectral range [2].
The use of near-IR radiation falling within the transparency window of biological tissues NIR-I (625-975 nm) [3] for excitation of plasmon resonance provides an advantage over other spectral ranges, since it is less absorbed by the main chromophores: melanin, hemoglobin, and water. However, due to light scattering in skin tissues, the penetration depth of laser radiation decreases. Optical cleaning is an effective means of increasing the effectiveness of PTT. [4].
In this study, the combined use of an immersion agent with low-intensity laser irradiation for optical clearing of the skin before the procedure of plasmon photothermal therapy (PPTT) is suggested. The pilot results of the study of the effect of immersion agents on the optical parameters of the skin, subcutaneous connective tissue and model tumor in rats in vivo with hyperthermia during PPTT are presented. A model of alveolar liver cancer - cholangiocarcinoma transplanted subcutaneously was used as a model tumor. Gold nanorods with an absorption band in the area of diode laser radiation (808 nm) were injected into the tumor. Measurements of the optical parameters of the whole tumor and its layers were carried out using spectrometers in the wavelength range 350-2200 nm. A reduction in thermal damage of the skin was obtained during PPTT with preliminary optical clearing using an immersion agent (a mixture of 70% aqueous glycerol solutions with 5% and 10% DMSO) and low-intensity laser irradiation at a wavelength of 808 nm.
The reported study was funded by the grant of RFBR (#20-52-56005) and the grant under the Degree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021).
[1] H.S. Jung, P. Verwilst, A. Sharma, J. Shin, J.L. Sessler, J.S. Kim, Organic molecule-based photothermal agents: an expanding photothermal therapy universe, Chem. Soc. Rev., vol. 47, pp. 2280-2297 (2018).
[2] X. Huang, P.K. Jain, I.H. El-Sayed, M.A. El-Sayed, Plasmonic photothermal therapy (PPTT) using gold nanoparticles, Lasers Med. Sci., vol. 23, pp. 217-228 (2008).
[3] A.N. Bashkatov, K.V. Berezin, K.N. Dvoretskiy, M.L. Chernavina, E.A. Genina, V.D. Genin, V.I. Kochubey, E.N. Lazareva, A.B. Pravdin, M.E. Shvachkina, P.A. Timoshina, D.K. Tuchina, D.D. Yakovlev, D.A. Yakovlev, I.Yu. Yanina, O.S. Zhernovaya, VV. Tuchin, Measurement of tissue optical properties in the context of tissue optical clearing, J. Biomed. Opt., vol. 23, pp. 091416-1 -091416-31 (2018).
[4] J.-I. Youn, The effect of an optical clearing agent on tissue prior to 1064-nm laser therapy, Med. Laser, vol. 10, pp. 146-152 (2021).
ALT'22
