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THE RESULTS OF THE STUDY OF ANTI-INFLAMMATORY ACTION OF THE GEL POLYGONUM AVICULARE, CHAMOMILE, CORTEX QUERCUS
Saydaliyeva Feruza Avazxanovna, Umarova Firuza Alisherovna, Ramazonova Shahzoda Shoyim qizi, Kozimjonova Nasiba Saminjonqizi, Normatov Sanjar, Tashkent Pharmaceutical Institute, Tashkent, Uzbekistan
E-mail: fira-umarova@yandex.ru
Abstract. Polygonum aviculare, chamomile, cortex quercus have been made for the use of skin inflammatory diseases. The pharmacological and microbiological properties of the prepared gel were investigated and the results obtained
Key words: inflammation, anti-inflammatory, antimicrobic activity, pharmacological activity, medicinal plants, gel, sodium carboxymethyl cellulose (Na-KMC).
Introduction. Inflammation (from latin: inflammatio) is part of the complex biological response of body tissues to harmful stimuli, such as pathogens, damaged cells, or irritants, and is a protective response involving immune cells, blood vessels, and molecular mediators. The function of inflammation is to eliminate the initial cause of cell injury, clear out necrotic cells and tissues damaged from the original insult and the inflammatory process, and initiate tissue repair. The five classical signs of inflammation are heat, pain, redness, swelling, and loss of function (Latin calor, dolor, rubor, tumor, and function laesa). Inflammation is a generic response, and therefore it is considered as a mechanism of innate immunity, as compared to adaptive immunity, which is specific for each pathogen Too little inflammation could lead to progressive tissue destruction by the harmful stimulus (e.g. bacteria) and compromise the survival of the organism. In contrast, chronic inflammation is associated with various diseases, such as hay fever, periodontal disease, atherosclerosis, and osteoarthritis^]. Chamomile or camomile is the common name for several daisy-like plants of the family Asteraceae. Two of the species are commonly used to make herbal infusions for traditional medicine, although there is no evidence that chamomile has any effect on health or diseases. Chamomile flowers contain essential oil, consisting of a main active ingredient - chamazulene, prohamazulen and other monoterpenes and sesquiterpenes. Of sesquiterpenes lactones are essential matrices and matrikarin. In flowers of plants contains flavonoids, coumarins, sitosterol, choline, carotene, ascorbic
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acid, isovaleric acid, and other organic and polysaccharides. Chamomile flowers are used as a tincture or in challenging fees to treat many diseases. Thus, the infusion of chamomile is used as an anti-inflammatory, antiseptic and analgesic for diseases of the mouth (stomatitis, gingivitis, etc.), tonsillitis and sore throat.[1,2,3]
Cortexquercus (Quercusrobur), also known as white cortex quercus, comes from the bark of a tree in the Fagaceae family. The bark from the cortex quercus tree is the only part that is used medicinally; cortex quercusis harvested from March to April. White cortex quercusis recognized as an herbal remedy that is generally safe, being listed on the GRAS list — meaning "Generally recognized to be safe" by the FDA. The German Commission E has approved the use of cortex quercus for the treatment of diarrhea, and it has been listed on the US Pharmacopoeia since 1916 for its astringent and antiseptic qualities. Found in cortex quercus tannins, flavonoids (quercetin, etc.), gallic acid and ellagic acid flobafen, pentosans, pectins, sugars, mucilage, starch and proteinaceous substances. The use of cortex quercusis due to its astringent, hemostatic, anti-inflammatory and soothing properties [3].
Polygonum Aviculare L., common name for plants in several genera in the family Polygonaceae. Polygonum Aviculare L or common knotgrass is a plant related to buckwheat and dock. It is also called prostrate knotweed, birdweed, pigweed and lowgrass. It is an annual found in fields and wasteland, with white flowers from June to October. It is widespread across many countries in temperate regions, apparently native to Eurasia and North America, naturalized in temperate parts of the Southern Hemisphere. Polygonum Aviculare contains the flavonols avicularin, myricitrin, juglanin, astragalin, betmidin and the lignin aviculin.
In scientific medicine Polygonum Aviculare herb is used as a tonic, diuretic and astringent. The plant is used as an astringent and styptic, and especially with uterine bleeding hemorrhoids as a hypotensive agent and vitamins [3].
Material and methodology. The liquid extracts had been obtaining from the method percolation. For percolation had taken 50 gram each herb and put into the flasks separately. With 50-100 % volume extracting the herbs wetted for four hours. After that time the wetted material had put into the percolator and spilled with extracting till appearing vitreous layer. Then we left it for a day. After 24 hours we began the percolation. When we obtain 85% of extract we put it in another flask and continued the percolation till the extract will become colorless and this fluid part we put in another flask. Diluted liquid evaporated with vacuum evaporator till deriving heavy-bodied mass. The heavy- bodied mass mixed with sorbitol (calcium stearate) and dried in the drying bake during 20 minutes.
The next stage of work was the development of the gel composition. Below is the composition of the gel obtained with two extracts.
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For obtaining gels separately had taken: For obtaining mixed gel had taken:
Na-KMC 7,5 g Na-KMC 7,5 g
Dry extract - 10 g (after solubiling with 10 ml ethanol alcohol) Dry extract - 10 g (each extract mixed together and from it had taken 10 g dry extract )
Glycerin 20 g Glycerin 20ml
Nipagin 1,5 g Nipagin 1,5 g
EASM (ethanolammonium cetilmaleinat salt) - 0,1 g EASM (ethanolammonium cetilmaleinat salt) - 0,1 g
bring to 100 grams of 5 % alcohol solution bring to 100 grams of 5 % alcohol solution
The microbiological activity of gels: Antimicrobic activity of gels studied diffusion methods in an agar and serial cultivations on a firm nutrient medium. As the test strains used cultures of Streptococcus pyogenes, Escherichia coli, Candida albicans, Aspergellius niger, A. fumigatus, A. flavum. To each species of microorganisms applied the corresponding nutrient mediums: meat peptonny agar, vitelline-salt agar, blood agar and Saburo's circle.
The pharmacological activity of mixed gel: The pharmacological activity of obtained gels investigated on 12 laborotorical rats with 140-165 g weight. Experimental arthritis- inflammatory was founded with the 2% of solution formalin. The formalin was conducting into the aponeurosis of rats foot. Rats divided into 2 groups. From the beginning until the fifth hour of experiments were measured every hour and measured 24 hours after. Every 8 hours investigated gel smudge to the foot of rats. Inspection continued 3 days. The legs of rats which in the control groups smudged with 70% ethanol alcohol.
Results of the microbiological activity of gels: Studying of sensitivity microorganisms E. coli, S. aureus, S. pyogenes, P. aerugenosa, C. albicans, A. niger, A. fumigatus, A. flavus to gels a diffusion method in an agar showed unequal sensitivity. The most sensitive appeared S. aureus and Streptococcus pyogenes the growth inhibition zone round holes made 21 mm. Were less sensitive A. niger, A. fumigatus, E. coli: Microorganisms with average sensitivity treated S. aureus, S. pyogenes, C. albicans, A. niger, A. flavus the growth inhibition zone round holes made 16 mm. P. aerugenosa was steady against to gels.
Results of the pharmocological activity of gels: The volume of rat legs in the control group were 0,45 ml, the volume of rat legs in the inspected group were 0,31 ml. Thus, the gel reduced inflammatorial process in the legs of rats 31,2%.
Studied the antimicrobic and pharmacological properties of gels. Antimicrobial study and pharmacological properties of the gels against inflammatory processes mouth and skin gave satisfactory reading.
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Conclusions: Liquid extracts obtained according to the method of percolation and receiving liquid extracts evaporated and dried for getting dry extracts. On the base of the dry extracts developed gels.
References:
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