CC BY
3The 30th International Conference on Advanced Laser Technologies B-I-37
ALT'23
The peculiarities of photoacoustic signals from CTCs, tumors and blood vessels of immunocompetent mice
Daniil Bratashov1, Olga Sindeeva2, Oleg Grishin1, Ekaterina Prikhozhdenko1, Olga
Guslyakova1, Olga Inozemtseva1
1- Saratov State University, 410012 Saratov, Russian Federation 2- Skolkovo Institute of Science and Technology, 121205 Moscow, Russian Federation
bratashovdn@info. sgu. ru
Two in vivo flow cytometry systems were created - a photoacoustic flow cytometer with a tunable laser wavelength and a light sheet-based cytometer with the possibility of magnetic separation of objects from the bloodstream. For a minimally invasive analysis of the presence of circulating tumor cells in the bloodstream, a photoacoustic flow cytometer was mainly used. For circulating melanoma tumor cells, after inoculation of B16-F10 cells into the thigh of a C57BL/6 mouse and injection into internal organs (liver, kidney, spleen), the number of formed circulating tumor cells (CTCs) was studied in the large vessel of animal limb. At the same time, the spread of metastasis in internal organs was studied using photoacoustic tomography. Using the technique of photoacoustic flow cytometry, it was shown that these models form a large number of CTCs in large vessels of the limb of a laboratory animal. The complete study of the process of metastasis was carried out by in vivo flow cytometry, photoacoustic imaging, cryosection analysis, and histological analysis. The number of circulating objects in the bloodstream was obtained for several weeks of tumor growth.
This work was supported by RSF project 18-19-00354 and Russian government project (megagrant) number 075-15-2021-617.
