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Atajanova Nargis Masharibovna, doctor, Urgench filial of Tashkent medical academy ministry of health Republic of Uzbekistan, Urgench Batyrbekov Akram Anvarovich, doctor of medical sciences, professor Institute of immunology and human genomics academy of science Republic of Uzbekistan, Tashkent E-mail: shga2065@yandex.ru
THE INFLUENCE GLISIROFITE AND HIS COMPONENTS ON ORGANS IMMUNITY AT SECONDARY IMMUNE DEFICITS
Abstract: It was established, that Glisirofite and components, enter in his composition - extracts of stock-rose and Radix Glycyrrhizae ability on 14-45 per cent (p < 0.05) increase number cells in central (thymus, bone morrow) and peripheral (spleen, lymph node) organs immunity at secondary immune deficits conditions: acute toxic hepatitis, radiation sickness.
Keywords: glisirofit, acute toxic hepatitis, radiation sickness, thymus, bone mor-row, spleen, lymph nodes.
It is known that plants contain various chemicals that have 5 groups. 1) group - control, intact mice; 2) RS; 3) RS + stockrose extract at a dose of 100 mg/kg; 4) RS + glycyram at a dose of 100 mg/kg; 5) RS + Glycyrophyte at a dose of 100 mg/kg. Results and discussion
First step was to study the effect of herbal remedies on the total number of cells in the central and peripheral organs of immunity in ATH (Table 1). In the control group, an average of 38.3 ± 1.7 x 106 thymocytes is recorded in the thymus. When ATH under the influence of CCl4, the total number of thymic cells decreases 1.80 times and is 21.3 ± 0.9 x 106. It has been established that under the influence of all three studied herbal remedies there is a significant increase in the total number of cells in the thymus of animals with ATH, and the stimulating activity of the agents did not differ from each other. Under the influence of the stock-rose extract, the number of thymocytes increases by 1.26 times, Radix Glycyrrhizae extract - by 1.30 times, Glycyrophyte - by 1.35 times.
Studies of the effect of herbal remedies on the proliferation of cells in the bone marrow of ATH mice gave the following results. In the control group, the bone marrow contains an average of 12.1 ± 0.4 x 106 cells. When ATH the number of cells decreases by 1.59 times. In mice with ATH, receiving stock-rose extract and licorice root extract, the number of bone marrow cells significantly increased by 1.24 times and 1.14 times, respectively. A mixture of these extracts has a greater stimulating activity than its original components. Thus, the number of bone marrow cells is 10.1 ± 0.2 x 106, which is 1.33 times higher than in the immune deficient group. In its activity, Glycyrophyte significantly exceeds the activity of licorice root extract, but does not differ in activity from stock-rose extract.
different biological effects and are used in various pathological conditions [4-6], including radiation injuries [8; 9]. The object of research is Glycyrophyte, which consists of stock-rose extract and extract Radix Glycyrrhizae, which contains glycyr-rhizic acid (HA) - triterpene glycoside, which is the main bioac-tive component and exhibits various types of pharmacological activity, including antiviral and antitumor. Earlier it was found that stock-rose has an expectorant effect [1], and HA has antiasthma [2], anti-ulcer [3], immunemodulatory [10] effects, and is used in the treatment of chronic hepatitis C [7].
The aim of the study is to estimation the effect of Glycy-rophite on the organs of immunity in secondary immune deficiencies.
Material and methods
In experiments, outbred mice of 2-3 months age body weight 20-22 g of both sexes were used. To simulate acute toxic hepatitis (ATH), mice for 3 days subcutaneously injected into the thigh area, 0.2 ml of 20% oily solution of the hepa-totropic poison - carbon tetrachloride (CCl4) was injected. After 5 days, the total number of nucleated cells of the spleen (NCS) was counted, as well as in the thymus, bone marrow, mesenteric lymph nodes. The immunomodulating properties of 3 herbal remedies were studied: 1) dry extract of stock-rose;
2) dry extract of Radix Glycyrrhizae (preparation glycyram);
3) Glycyrophyte - a mixture of extracts of stock-roses and Radix Glycyrrhizae in a 1:1 ratio. All herbal remedies were administered at a dose of 75 mg/kg intragastrically for 4 days.
To simulate radiation sickness (RS), mice were irradiated at a dose of 5 Gr. After 5 days, herbal remedies were intragastrically administered to mice for 4 days. Animals were divided into
THE INFLUENCE GLISIROFITE AND HIS COMPONENTS ON ORGANS IMMUNITY AT SECONDARY IMMUNE DEFICITS
Table 1 shows the data on the calculation of the number ATH. In the control group, this indicator is equal to 169.7 ± of NCS when administering the studied agents to mice with ± 7.2 x 106.
Table 1.- Effect herbal remedies on number cells in organs immunity at mice with acute toxic hepatitis (ATH) (M±m)
Central organs
Group Dose, mg/kg Thymus' cells x 106 IR Bone marrow cells x 106 IR
1. control (n=7) - 38.3 ± 1.7 - 12.1 ± 0.4 -
2. ATH (n=7) - 21.3 ± 0.9a -1.80 7.6 ± 0.3a -1.59
3. ATH + extract of stock-rose (n=7) 75.0 26.8 ± 1.2ab + 1.26 9.4 ± 0.3ab + 1.24
4. ATH + extract Radix Glycyrrhizae (n=7) 75.0 27.7 ± 1.2ab + 1.30 8.7 ± 0.3ab + 1.14
5. ATH + Glisirofite (n=7) 75.0 28.7 ± 1.2ab + 1.35 10.1 ± 0.2ab + 1.33
Peripheral organs
Group Dose. mg/kg Number of NCS x 106 IR Lymph nodes cells x 106 IR
1. control (n=7) - 169.7 ± 7.2 - 20.3 ± 0.5 -
2. ATH (n=7) - 105.8 ± 4.5a -1.60 11.9 ± 0.3a 1.71
3. ATH + extracts of stock-rose (n=7) 75.0 121.7 ± 5.2ab + 1.15 15.1 ± 0.3ab + 1.27
4. ATH + Radix Glycyrrhizae (n=7) 75.0 127.0 ± 5.4ab + 1.20 14.4 ± 0.4ab + 1.21
5. ATH + Glisirofite (n=7) 75.0 132.3 ± 5.6ab + 1.25 16.2 ± 0.3ab + 1.36
Note: here and in the tables N2, NCS - nucleated cells of the relation to 2 gr., a - authentically to 1 gr., b - authentically to 2g.,
In all groups treated with herbal remedies, a significant increase in the total number of spleen cells was observed. With the introduction of the stock-rose extract, the number of NCS is increased by 1.15 times, Radix Glycyrrhizae extract - by 1.20 times, Glycyrophyte - by 1.25 times. In their stimulating activity, all three herbal remedies do not differ from each other. The results obtained indicate the ability of the studied samples to increase the immunological reactivity of the organism and the total number of cells in the spleens of mice with ATH.
The stimulating effect of herbal remedies was also found for the mesenteric lymph nodes (Table 1). In the control group, the number of cells in the lymph nodes is 20.3 ± 0.5 x 106, while with ATH their number decreases by 1.71 times. With the introduction of herbal remedies to animals with liver pathology, an increase in the number in the lymph nodes is observed. And by the strength of the activity, they do not differ from each other. So, under the influence of stock-rose, the number of cells in lymph nodes increases 1.27 times, Radix Glycyrchizae - 1.21 time and Glycyrophyte - 1.36 times.
Based on the obtained results, it can be concluded that the studied herbal remedies have the ability to exert a stimulating effect on the central and peripheral organs of the immune system in mice with ATH.
After radiation exposure, negative changes occur in the organs of immunity (Table 2). Thus, the number of cells in
spleen; IR - the index of the ratio: (-) - in relation to 1 gr., (+) - in c - authentically to 3gr., d - authentically to 3gr
the thymus decreases by 3.15 times, and the bone marrow -by 2.92 times. Under the influence of stock-rose extract, the level of cells in the organs of immunity of irradiated mice increases: in the thymus - 1.25 times, in the bone marrow -1.21 times. Similar results were obtained with the administration of glycyram to irradiated animals: the number of cells in the thymus increased 1.21 times, in the bone marrow -1.18 times. More pronounced stimulating activity was found in Glycyrophyte: the number of cells in the thymus increased 1.45 times, in the bone marrow - 1.29 times. As in previous studies, Glycyrophyte in its immune-stimulating activity significantly exceeds that in extracts of stock-rose and glycyram.
Similar data were obtained in peripheral organs of immunity in mice with RS. As can be seen from table 2, the number of NCS in irradiated animals decreases by 4.12 times compared with the control. In groups of mice treated with stock-rose extract and glycyram, the number of NCS increased 1.19 and 1.24, respectively. Under the influence of Glycyrophite, the number of NCS increases 1.42 times. In its activity, Glycyrophyte significantly exceeds that of stockrose and glycyram extract. Based on the data obtained, it can be concluded that Glycyrophyte and its components are able to a certain extent increase the total number of cells in the spleens of irradiated mice.
Table 2.- Effect herbal remedies on number cells in organs immunity at mice with radiation sickness (RS) (M±m)
Central organs
Group Dose, mg/kg Thymus' cells x 106 IR Bone marrow cells f IR
1. control (n=8) - 44.4 ± 1.1 - 11.1 ± 0.3 -
2. RS (n=8) - 14.1 ± 0.4a -3.15 3.8 ± 0.1a -2.92
3. RS + extracts of stock-rose (n=8) 100.0 17.6 ± 0.5ab + 1.25 4.6 ± 0.1ab + 1.21
4. RS + glisiram (n=8) 100.0 17.0 ± 0.5ab + 1.21 4.5 ± 0.1ab + 1.18
5. RS + Glisirofite (n=8) 100.0 20. 4± 0.6abcd + 1.45 4.9 ± 0.1abd + 1.29
Peripheral organs
Group Dose, mg /kg Number of NCS x 106 IR Lymph nodes cells f IR
1. control (n=8) - 150.4 ± 4.1 - 26.5 ± 0.7 -
2. RS (n=8) - 36.5 ± 1.0a -4.12 9.5 ± 0.3a -2.80
3. RS + extracts of stock-rose (n=8) 100.0 43.4 ± 1.2ab + 1.19 12.1 ± 0.3ab + 1.27
4. RS + glisiram (n=8) 100.0 45.2 ± 1.2ab + 1.24 11.7 ± 0.3ab + 1.23
5. RS + Glisirofite (n=8) 100.0 51.8 ± 1.4abcd + 1.42 12.9 ± 0.3abd + 1.36
After irradiation, the number of cells in the lymph nodes decreases 2.80 times. All the studied herbal remedies significantly increase the number of cells in the lymph nodes: stock-rose extract by 1.27 times, glycyram - by 1.23 times, Glycyrophyte - by 1.36 times.
Thus, Glycyrophyte and its source components can increase the number of cells in the central (thymus, bone marrow) and peripheral (spleen, lymph nodes) organs of immunity in secondary immunodeficiency states (acute toxic hepatitis, radiation sickness).
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