CC BY
6DOI 10.24412/cl-37136-2023-1-57-57
THE FORCES OF INTERACTION OF RED BLOOD CELLS AND ENDOTHELIUM: A REVISED STUDY BY LASER TWEEZERS
PETR B. ERMOLINSKIY1*, MATVEY K. MAXIMOV1, ANDREI E. LUGOVTSOV1, OLGA N.
SCHEGLOVITOVA2 AND ALEXANDER V. PRIEZZHEV1
1Faculty of Physics, Lomonosov Moscow State University, Moscow, Russia 2N.F. Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia
* Correspondence: [email protected]
ABSTRACT
Blood microbiology is essentially determined by the properties of blood plasma and the interaction between blood cells, i.e., the aggregation of red blood cells (RBCs), the interaction between different blood cells and endothelium, etc. [1]. Endothelial cells do not only act as an insulating layer between blood and tissues, but also play an important role in controlling the blood flow and influence blood cell properties, e.g., RBCs aggregation. RBCs can reversibly interact with each other under low shear stress forces forming linear and more complex structures. RBCs aggregation is mainly responsible for the non-Newtonian behavior of blood and regulates the microcirculation of blood in human body.
In this work, we studied the interaction between the endothelial monolayer and RBCs. Previously, we studied the interaction between single endothelial cells and single RBCs. However, a single endothelial cell and an endothelial cell in a monolayer have different properties.
The aim of this study was to investigate the interaction between endothelium cells in a monolayer and RBCs of healthy volunteers at different concentrations of fibrinogen at the level of individual cells in vitro. Laser tweezers were used to manipulate individual cells without mechanical contact, as well as to measure the forces of their interaction [2].
Blood for the study was drawn from the cubital veins of four healthy donors. Endothelial cells were grown at 37°C (CO2 environment) on round glass slides and formed a cell monolayer. The blood sample was a small amount of blood and autologous serum with added fibrinogen under the concentrations of 0, 2, 4, 6, 8 mg/ml. The blood sample was placed in a microcuvette with endothelium isolated from the air and the interaction forces between the cells were measured.
It was shown that the interaction force between RBCs and endothelial cells increases with fibrinogen concentration and reaches the saturation level of 4 pN at the concentration of 4 mg/ml. This force value is comparable with the interaction forces between individual RBCs in autological plasma.
This work was supported by the Development program of the Interdisciplinary Scientific and Educational School of Lomonosov Moscow State University «Photonic and Quantum Technologies. Digital medicine».
REFERENCES
[1] Furst E.M., Squires T.M. "Microrheology" Oxford University Press, United Kingdom (2017).
[2] Kapkov A.A., Semenov A.N., Ermolinskiy P.B., Lugovtsov A.E., Priezzhev A.V. "Forces of RBC interaction with single endothelial cells in stationary conditions: Measurements with laser tweezers". Journal of Innovative Optical Health Sciences. 14(5), 2142005 (2021).
