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Section 7. Medical science
4. Никитушкин В. Г. Современная подготовка юных спортсменов//Москомспорт -справочник и методическое пособие. - Москва, 2009. - № 1. - С. 22-41.
5. Погадаев Г. И. Настольная книга учителя физической культуры. - Москва: Физкультура и спорт, 2000. - 496 с.
Isanova Dilfuza Tursunovna, Senior scientific employee-researcher of the Department of Infectious Diseases, Epidemiology and Microbiology of Andijan State Medical Institute, Andijan, Uzbekistan
Azizov Yuriy Dalievich, MD, professor of the Department of Infectious Diseases, Epidemiology and Microbiology of Andijan State Medical Institute, Andijan, Uzbekistan E-mail: [email protected]
The estimation of the variability of the taxonomic characteristics of patogens of urinary tract infections in women
Abstract: The aim was a comparative study of the variability of taxonomic characters sown E.coli strains from women with acute and chronic cystitis. It was found that E.coli strains isolated from different habitats of the body healthy and sick women with varying frequency detected taxonomic characteristics, which are associated with the adhesiveness of the pathogen. Strains were sown in women with acute and chronic cystitis, variability exhibited by fermentation mannitol, sucrose, arabinose, glucose, sodium citrate, hemolytic activity.
Keywords: taxonomic characteristics, variability, urinary tract infections, pathogens, E.coli.
Many of the major taxonomic characteristics (TCh) of organ- = 84) ChC. The diagnosis was verified by modern clinical and inisms do not change in normal growth conditions, but under the in- strumental, laboratory methods and confirmed by bacteriological fluence of various unfavorable factors the properties change [1]. examination.
Many opportunistic pathogens bacterium (OPB) were acquired After the identification of isolated cultures for further in-depth
pathogenic properties namely under these conditions [5]. It is proved that the long persistence of the pathogen in the body leads to a chronic process of infectious-inflammatory process and change some of the properties of this organism [2; 6; 7].
The researchers note that the leading causative agents of urinary tract infections (UTI) are the E.coli [3; 8].
In uropathogenic of E.coli, isolated from women with acute cystitis virulence was, the higher was the most resistant to ciprofloxacin and of E.coli, associated with cystitis women, in contrast to of E.coli, isolated from healthy individuals treated particularly severely limited clonal group A-CGA [9].
At the same time, according to other authors, there is a shift in the etiology of acute (AC) cystitis recent years. Thus, some authors believe that there is replacement ofE.coli to other En-terobacteriace-ae, with an increase in their share to 21.8% [2] and even up to 60.8%.
It is still not resolved is the degree of changes of taxonomic characters uropathogenic microorganisms. In this regard, the study of basic taxonomic features of human pathogens of UTI, including women of childbearing age in the dynamics of the disease, monitoring ino-culation OPB is relevant.
Aim of the study. Comparative study and evaluation of the variability of the main ta-xonomic E.coli signs that sown by women of childbearing age with UTI.
Materials and methods. To accomplish this goal were studied 1026 women's urine sam-ples UTI patients (age 18-49 years), of which 27.1 ± 1.4% (n=278) cases of urine samples col-lected from women of AC and chronic cystitis (ChC). Of these, 69.8 ± ± 2.8% (n=194) ofwomen diagnosed with AC and 30.2 ± 2.8% (n
research on bacteriological variability taxonomic features 29 E.coli strains were used (15 from women with AC and 14 from women with ChC) at > 1h105 CFU/ml in urine. For comparison, 21 E.coli strain is seeded from the feces of healthy women (control group).
All bacteriological tests for the identification and differentiation of strains were per-formed in the same conditions using conventional bacteriological methods [4].
Statistical processing of the obtained materials was performed by variation statistics using the application package for biomedical research. When organizing and conducting research using the principles of evidence-based medicine.
Results and discussion. We found that out of 278 AC and ChC E.coli female patients urine samples were sown in 73.9 ± ± 2.6% of patients. However, often plated with E.coli other representatives of the family Enterobacteriaceae, genera Staphylococcus spp, Streptococcus spp, Enterococcus spp, Pseudomonas spp and Candida spp.
Further identification of crops showed the homogeneity of microbial genera Escherichia spp., Pseudomonas spp., Staphylococcus spp. on the main TCh. Due to the fact, that often plated E.coli, the special microbiological studies were carried out with the strains of these microor-ganisms.
It is known, that the hemagglutinating strains activity characterizes their adhesiveness, as one of the bacterial pathogenicity factors [1]. Study of the total activity hemagglutinating strains of E.coli seeded from urine of patients with ChC showed that 85.7 ± ± 9.3% of cases (n = 12), they possess these properties. This parameter was significantly superior parameters strains (P<0.002) seeded
The estimation of the variability of the taxonomic characteristics of patogens of urinary tract infections in women
from urine of women with AC (53.3 ± 12.9%; n=8) and control group (23.8 ± 9.3%; n = 5) (P<0.001).
Received respectively 1.6 and 3.6 fold increase in total hemag-glutinating activity of E.coli cultures obtained from women with ChC indicates increased pathogenicity of these strains in comparison with AC and the control group (P <0.05).
In addition, there were significant differences in the amount of E.coli strains isolated from urine of women AC and ChC (n=15, respectively, and n=14) and control group (n=21) having only mannoza resistant haemagglutinin (P<0.001), and the combination mannoza re-sistant and mannoza sensitive hemagglutinin (P<0.05), where the rates were greater in the strains from patients sown. Mannoza sensitive hemagglutinin study showed that their presence between strains of E.coli, isolated from urine of women and female patients in the control group were ob-served significant differences (P>0.05).
Further studies have been devoted to the study of the biochemical properties of strains seeded from patients and healthy women.
It established that E.coli strains fermented carbohydratesin different: glucose, lactose, mannitol, maltose, arabinose, glucose Na citrate by 93.3 ± 6.5% (n=14) to 100% but not fer-mentation (0%), inositol, sorbitol and malonate Na. According to these indicators, reliable differences in the compared groups of women was observed (P>0.05).
There were differences in the ability to utilize sucrose. Strains of healthy women 95.2 ± 4.7% (n=20) cases, the strains from women patients with BMP in 97.4 ± 7.8% (n=14) of cases (P>0.05) strains of female patients with ChC in 35.7 ± 12.8% (n=5) of cases (P<0.01) had the ability to utilize sucrose.
In other studied biochemical parameters revealed differences were small and statistically no significant difference (P>0.05) the studied strains, for this reason, we do not mark them as a distinct taxonomic characteristics of the strains.
Indicators of the proteolytic activity of strains studied differed from the above-mentioned parameters of carbohydrate utilization (biochemical markers). It was found that, regardless of the origin of all isolated E.coli strains did not produce hydrogen sulfide, lacked arginindegidrolase, fenilalanindezaminase, and urease-galactosidase activity.
All E.coli strains were not possess hemolytic activity than strains isolated from urine ofwomen with ChC (28.6 ± 12.1%; n=4).
Thus, it is revealed that the strains of E.coli seeded from urine of women patients with AC andChC were exhibited a variability by the following taxonomic characteristics: fermenta-tion of mannitol, sucrose, arabinose, glucose with Na citrate, Education indole, hemolysin and antigenic properties with respect to strains of the control group.
Apparently, the variability of taxonomic characters E.coli strains isolated from the urine of women with AC and ChC depended on getting this pathogen in other biotope (genitourinary tract), adaptation to these conditionsand the long persistence in the body.
The obtaining results in the process of in-depth research uro-pathogenic bacteriological results are of great importance in diagnosis, predicting outcomes and assessing the occurrence and course of UTI in women of childbearing age due to E.coli.
Conclusions:
1. Respectively 1.6 and 3.6 fold increase in total hemagglutinat-ing activity of E.coli cultu-res obtained from women with ChC indicates increased pathogenicity of these strains in compa-rison with AC and the control group (P<0.05).
2. E.colistrain seeded from urine of women patients with AC and ChC were exhibited va-riability by the following taxonomic characteristics: fermentation of mannitol, sucrose, arabi-nose, glucose with Na citrate and hemolytic activity.
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