The Effects of storage on germination characteristics and enzyme activity of sorghum seeds Текст научной статьи по специальности «Сельское хозяйство, лесное хозяйство, рыбное хозяйство»

Journal of Stress Physiology & Biochemistry, Vol. 9 No. 4 2013, pp. 289-298 ISSN 1997-0838 Original Text Copyright © 2013 by Azadi, Younesi

ORIGINAL ARTICLE

The Effects of Storage on Germination Characteristics and Enzyme Activity of Sorghum Seeds

Azadi M.S.1, Younesi E.2*

1 Department of Agronomy and Plant Breeding, Islamic Azad University, Dezfoul Branch, Iran.

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran.

*E-Mail: younesiehsan@ut.ac. ir

Received July 23, 2013

Seed moisture content (MC) and storage temperature are the most important factors affecting seed longevity and vigor. Exposure to warm, moist air is principally responsible for this. Proper storage and optimum seed moisture content can affect the grain quality significantly. The purpose of this study was to evaluate the different storage treatments on seed quality of sorghum. The seed materials were fresh without any storage period. For storage treatments, 3 seed moisture contents (6, 10, 14 %) were stored for 8 month in 0.5 L capacity sealed aluminum foil packet in 0.3 bar inside incubators set at 4 temperatures (5, 15, 25, 35°C). After storage time, the higher the storage temperature, the lower was the grain quality of sorghum. The highest germination percentage, germination index, normal seedling percentage were achieved in control conditions (0 day of storage). Our results showed that increasing storage duration resulted higher reduction in germination characteristics. Also our results showed that, germination percentage, means time to germination, germination index, normal seedling percentage decrease significantly by storage. Enzyme activity decrease significantly by increased in storage.

Key words: Storage, Germination characteristics, Sorghum, enzyme activity.

290

The Effects of Storage on Germination Characteristics..

ORIGINAL ARTICLE

The Effects of Storage on Germination Characteristics and Enzyme Activity of Sorghum Seeds

Azadi. M.S.1, Younesi. E.2*

1 Department of Agronomy and Plant Breeding, Islamic Azad University, Dezfoul Branch, Iran.

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj, Iran.

*E-Mail: younesiehsan@ut.ac. ir

Received July 23, 2013

Seed moisture content (MC) and storage temperature are the most important factors affecting seed longevity and vigor. Exposure to warm, moist air is principally responsible for this. Proper storage and optimum seed moisture content can affect the grain quality significantly. The purpose of this study was to evaluate the different storage treatments on seed quality of sorghum. The seed materials were fresh without any storage period. For storage treatments, 3 seed moisture contents (6, 10, 14 %) were stored for 8 month in 0.5 L capacity sealed aluminum foil packet in 0.3 bar inside incubators set at 4 temperatures (5, 15, 25, 35°C). After storage time, the higher the storage temperature, the lower was the grain quality of sorghum. The highest germination percentage, germination index, normal seedling percentage were achieved in control conditions (0 day of storage). Our results showed that increasing storage duration resulted higher reduction in germination characteristics. Also our results showed that, germination percentage, means time to germination, germination index, normal seedling percentage decrease significantly by storage. Enzyme activity decrease significantly by increased in storage.

Key words: Storage, Germination characteristics, Sorghum, enzyme activity.

Seed characteristics decrease under long storage condition due to aging. Changes that occur in seed during aging are significant in terms of seed quality, the feature that, among other things, also implies seed longevity (McDonald, 1999). Progress of the technology and industrialization of agricultural production has increased opportunities for long term storage of seed. Aging conditions generally reduce seed vigor (McDonald, 1999). The purpose of proper storage is to inhibit biological processes to the highest possible extent and to eliminate

unfavorable environmental factors, which limit duration of the safe storage. The biochemical processes occurring in grain are directly influenced by moisture content, air temperature, contact with air and condition of grain (degree of damage) (Siadat et al., 2012; Ghasemnezhad and

Honermeier, 2007). Seed deterioration can be defined as the loss of quality, viability and vigour either due to aging or effect of adverse environmental factors. The rate of deterioration rapidly increases in either seed moisture content or

Azadi, Younesi

temperature of storage (Kapoor et al., 2010). Decrease in seed vigour is due to decrease in seed quality, percentage, rate of germination and yield and also can increase susceptibility to environmental stress (Tekrony et al., 1989). Rising of temperature, humidity and oxygen pressure could cause structural damages on DNA and ribosomal RNA, increasing enzyme activity, respiration and membrane permeability (Mc Donald, 1999). The purpose of storage is to maintain harvest quality of product, not to improve it (Sisman and Delibas, 2004). The rate at which the seed aging process takes place depends on the ability of seed to resist degradation changes by protection mechanisms which are specific for each plant species. Loss of viability in crops is also attributed to non-congenial storage environmental factors like higher relative humidity and temperature during storage. In this context storage of seeds has been considered as most important as "seed saved is seed produced" Sorghum bicolor is the fourth most important world cereal grain, following wheat, rice, and corn. Seed quality (viability and vigor) can have a profound influence on the establishment and the yield of a crop. Parera and Cantliffe (1994) reported that rapid and uniform field emergence is an essential prerequisite to reach the yield potential, quality and ultimately profit in annual crops. Therefore, the objectives of this study were to determine how different seed storage methods and storage times affected the germination characteristics of sorghum seeds.

MATERIALS AND METHODS

Moisture content (%)

The moisture content of the seed was calculated as per the International Seed Testing Association (ISTA) Rules (ISTA, 2010) by hot air oven method

maintaining 130°C ± 2°C for 2 hr. The moisture content was calculated on wet basis and expressed in percentage by using the following formula:

(M 2 - M 3 )/ (M 2 - M 1 )x 100

Where,

M 1 = Weight of empty moisture tin (g)

M 2 = Weight of moisture tin and seed material before drying (g)

M 3 = Weight of moisture tin and seed material after drying (g)

Storage conditions

Seeds with 3 moisture contents (6, 10 and 14%) were exposed to 4 temperatures (5, 15, 25 and 35 °C) for six months. Seeds were taken every month was measured.

Standard germination test was carried out at 25°C for 10 days in three replications of 50 seeds. Seeds were germinated on two layers of filter papers (Whatman no. 1) moistened with 5 ml distilled water in petri-dishes. The germinated seeds (2 mm radicle elongation) were counted daily to calculate germination rate. At the end of the germination period, total and germination percentage, normal seedling percentage, germination index and mean time to germination were recorded.

RESULTS AND DISCUSSION

The results indicated that duration of storage (A), temperature (B), moisture contents (C), AxB, AxC, BxC and AxBxC interaction were significant (P < 0.01, 0.05) for all traits in seeds under storage (Tab 1). Our results showed that the highest germination percentage (Fig 1), normal seedling percentage (Fig 2), germination index (Fig 3) and the minimum, mean time to germination (Fig 4) were achieved under control conditions (0 day of storage). Therefor the minimum this traits were

The Effects of Storage on Germination Characteristics...

292

attained under 180 days of aging. Seed germination, normal seedling percentage, germination index reduced with increase in seed moisture content and storage temperature (Fig 1, 2 and 3), but mean time to germination increases by increase in seed moisture content and storage temperature (fig 4). The highest germination characteristics in sorghum seed was attained at 6% seed moisture contents at 5, 15 and 25 but reduced at 35 °C (Fig 1, 2, 3 and 4). Germination percentage of seeds with 6% moisture content stored at 5 °C after 180 days was 72%, while the germination percentage of seeds with 6% moisture content stored at 35 °C after 180 days was 44% and Germination percentage of seeds with 14% moisture content stored at 5 °C after 180 days was 60% (Fig 1 a), while the germination percentage of seeds with 14% moisture content stored at 35 °C after 180 days was 28% (Fig 1 c). The results of our study suggested that increases in storage caused a decreased in germination characteristics (Fig 1).

Increasing seed age decreased germination and this result is in accordance with Jan-Mohammadi et al. (2008) and Ghassemi-Golezani et al. (2010) in rapeseed and Saha and Sultana (2008) in soybean. Also, earlier reports (Bailly, 2004; McDonald, 2004) have shown negative effect of aging in relation to

seed performance, germination percentage and seedling indices.

According to Ghasemnezhad and Honermeir (2007), the storage life of sunflower seed can be reduced by long storage and high storage temperature.

Akhtar et al. (1992) suggested that decreasing in GP was related to chromosomal aberrations that occur under long storage conditions. Decreasing of GP in aged seeds can be due to reduction of a-amylase activity and carbohydrate contents (Bailly, 2004) or denaturation of proteins (Nautiyal et al., 1985). According to Abdalla and Roberts, (1968) barley and pea seeds treated with different combinations of accelerated ageing treatment showed that the amount of genetic damage was solely a function of loss of viability.

Also, our results showed that enzyme activity decreased in seeds after aging (Fig. 5 and 6). The results of our study suggested that increases in storage caused a decreased in enzyme activity (Fig. 5 and 6). Most of these studies suggest that decreases occur in the activity of enzymes in aged seeds (Bailly, 2004; Goel et al., 2002; McDonald, 2004). Kibinza et al. (2011) reported that the CAT is a key enzyme in seed recovery after aging.

Table 1. Analysis of variance temperature, moisture and storage on traits studied of sorghum seeds.

S.O.V df Germination percentage Normal seedling percentage Germination index Mean time to germination

Douration of storage (A) 5 6217.33** 12372.29** 428.17** 1.78**

Temperature (B) 3 2981.231** 3798.01** 401.35** 0.35**

Moisture contents (C) 2 1301.79** 1233.01** 83.95** 0.69**

A*B 15 141.51** 168.12** 4.05** .51 0.

A*C 10 51.11** 49.96** .51 2. *CN 0.

B*C 6 62.01** 62.94** 9.96** 0.65**

A*B*C 30 13.69* 15.08* 2.15** 0.11**

Error - 6.99 8.04 1.11 0.06

C.V% - 4.22 5.03 7.87 5.97

*and ** indicate significant difference at 5% and 1% probability level respectively.

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Figure 3. The effect of duration of storage x temperature x moisture contents interaction on germination index of sorghum seeds.

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Figure 5. The effect of duration of storage x temperature x moisture contents interaction on catalase activity of sorghum seeds.

Figure 6. The effect of duration of storage x temperature x moisture contents interaction on ascorbate peroxidase activity of sorghum seeds.

CONCLUSION

The higher the storage temperature, the lower was the grain quality of sorghum. The highest germination percentage, germination index, normal seedling percentage were achieved in control conditions (0 day of storage). Our results showed that increasing storage duration resulted higher reduction in germination characteristics and enzyme activity.

REFERENCES

Abdalla, F.H., Roberts, E.H. (1968) Effects of temperature, moisture and oxygen on the induction of chromosome damage in seeds of barley, broad beans and peas during storage. Ann Bot. 32: 119-136.

Akhter, F.N., Kabir, G., Mannan, M.A., Shaheen, N.N. (1992) Aging effect of wheat and barley seeds upon germination mitotic index and chromosomal damage. J Islam Acad Sci. 5: 44-

298

The Effects of Storage on Germination Characteristics...

48.

Bailly, C. (2004) Active oxygen species and antioxidants in seed biology. Seed Sci Res. 14: 93-107.

Ghasemi-Golezani, K., Salehian, H., Rahimzade-Khoee, F., Moghadam, M. (1996) The effect of seed vigor on seedling emergence and yield of wheat. Natural Resources and Agricultural Sciences. 3: 58-48.

Ghasemnezhad, A., Honermeier, B. (2007) Influence of storage conditions on quality and viability of high and low oleic sunflower seeds. International Journal of Plant Production. 3(4): 39-48.

Goel, A., Goel, A.K., Sheoran, I.S. (2002) Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. J Plant Physiol. 160: 1093-1100.

ISTA (2010) International Rules for Seed Testing. International Seed Testing Association, Switzerland.

Jan-Mohammadi, M., Fallahnezhad, F., Golsha, M., Mohammadi, H. (2008) Controlled ageing for storability assessment and predicting seedling early growth of canola cultivars (Brassica napus L.). ARPN J Agric Biol Sci. 3:22-26.

Kapoor, N., Arya, A., Siddiqui, M.A., Amir, A., Kumar, H. (2010) Seed deterioration in chickpea (Cicer arietinum L.) under accelerated aging. Asian J Plant Sci. 9(3):158-162.

Kibinza, S., Bazina, J., Bailly, C., Farrant, J.M., Corbineaua, O., Bouteaua, H. (2011) Catalase is a key enzyme in seed recovery from ageing during priming. Plant Science. 181: 309- 315.

McDonald, M.B. (1999) Seed deterioration: physiology, repair and assessment. Seed Science Technology. 27: 177-237.

McDonald, M.B. (2004) Orthodox seed deterioration and its repair, pp. 273-304. In: Handbook of Seed Physiology: Applications to Agriculture, Benech-Arnold, R. L. and R.A. Sanchez (Eds.). Food Products Press, New York.

Nautiyal. A.R, Thapliyal, A.P., Purohit, A.N. (1985) Seed viability. IV. Protein changes: Accompanying loss of viability in Shorea robusta. Seed Sci Technol. 13: 83-86.

Saha, R.R., Sultana, W. (2008) Influence of seed ageing on growth and yield of soybean. Banglad J Bot. 37: 21-26.

Siadat, S.A., Moosavi, A., Sharafizadeh, M. (2012) Effect of seed priming on antioxidant activity and germination characteristics of Maize seeds under different aging treatments. Research Journals of Seed Scienc. 5(2): 51-62.

Sisman, C., Delibas, L. (2004) Storing sunflower seed and quality losses during storage. Journal of Central European Agriculture. 4: 239-250.

TeKrony, D.M., Egli, D.B., Wickham, D.A. (1989) Corn seed vigour effect on notillage field performance. J Crop Sci. 29: 1523-1528.