CC BY
63
Ukrainian Journal of Ecology
Ukrainian Journal ofEcology, 2019, 9(3), 222-226
ORIGINAL ARTICLE
The effect of cadmium loading on protein synthesis function and functional state of laying hens' liver
B. Gutyj1, A. Ostapiuk1, N. Kachmar2, O. Stadnytska3, O. Sobolev4, V. Binkevych1, R. Petryshak1, O. Petryshak1, O. Kulyaba1, A. Naumyuk1, V. Nedashkivsky4, N. Nedashkivska4, N. Magrelo1,
I. Golodyuk1, N. Nazaruk1, O. Binkevych1
1Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Ukraine 2Lviv National Agrarian University, Dublyany, Ukraine 3Institute of Agriculture of the Carpathian region of the National Academy of Agrarian Sciences of Ukraine, Obroshino,
Ukraine
4BHa Tserkva National Agrarian University, Bila Tserkva, Ukraine E-mail: bvh@ukr.net
Received: 26.09.2019. Accepted: 26.10.2019
In the article are considered issues related to the study of the effect of cadmium on the body of birds, in particular laying hens. The effect of cadmium sulfate at doses of 2.0 and 4.0 mg/kg body weight on protein synthesis function and functional state of the liver were investigated of cross-laying Hysex white hens, of 78 weeks old. Three groups of chickens were formed: two experimental and one control groups. The laying hens of the experimental groups were subjected to a cadmium load. Laying chickens of the first experimental group were given cadmium sulfate at a dose of 2.0 mg/kg body weight. Laying chickens of the second experimental group were given cadmium sulfate at a dose of 4.0 mg/kg body weight. Laying chickens in the control group were in the normal diet without cadmium. Increased activity of aminotransferases, in particular alanine and aspartate aminotransferases in their serum of hens, was observed during cadmium binge drinking in doses of 2.0 and 4.0 mg/kg body weight, respectively. These changes indicate a violation of the functional state of the liver of laying hens under conditions of cadmium load. In the study of protein synthesis of the liver of laying hens under conditions of cadmium loading, it was found that cadmium sulfate in the above doses contributed to a significant decrease in the level of total protein by 14, 21 and 30 days of the experiment. The reduction of total protein in the blood of laying hens was due to a decrease in the level of albumin, which in the second experimental group, respectively, decreased to 28.16 ± 0.75%, whereas in the first - 29.62 ± 0.98%. In the study of the level of globulins, it is possible to increase this indicator in both experimental groups of poultry for 21 days. Drinking with water cadmium sulfate at a dose of 4.0 mg/kg body weight was associated with a more likely decrease in total protein and albumin than drinking cadmium sulfate at a lower dose.
Keywords: Toxicology; Cadmium; Hens; Blood; Immune system
Introduction
Uncontrolled excessive emissions of industrial enterprises and motor vehicles, violations of the fertilizer application system, the treatment of plants with pesticides, technological accidents and other anthropogenic factors lead to the accumulation of heavy metals in soil and plants, adversely affecting the health of agricultural animals and humans. (Uetani et al., 2005; Nazaruk et al., 2015; Sachko et al., 2016; Gutyj et al., 2016; Khariv et al., 2016). Over the past decades, an increase in the content of this metal in the soils of Ukraine and other countries has been accompanied by the accumulation of Cd2+ in agricultural products and feed, an increase in the threat to human and animal health. (Hutyi, 2013; Gutyj et al., 2015; Hradovych et al., 2016; Grushanska, 2017; Gutyj et al., 2017; Grynevych et al., 2018).
Cadmium and its compounds belong to the immunotoxicant, which cause disruptions in the functioning of the body's immune system, reduce resistance to infections, contribute to the formation of allergic, autoimmune and oncological pathologies (Ali et al., 1986; Salvatori et al., 2004; El-Refaiy and Eissa, 2012; Peng et al., 2015; Gutyj, 2015). A feature of the biological action of cadmium is its ability to adversely affect the health of animals with prolonged exposure to low levels of pollution due to the high coefficient of biological cumulation (up to 40 years). It is known that cadmium can significantly change the metabolism and functions of such essential elements as zinc, iron, copper, manganese, calcium, selenium (Ostapyuk & Gutyj, 2018; Gutyj et al., 2019).
Cadmium adversely affects the vital systems of humans and animals, causing pathological changes in tissues and organs (kidneys, lungs, bone tissue, organs of the reproductive and endocrine systems), inhibiting the process of erythropoiesis and immune system function (Fregoneze et al., 1997; Rodriguez et al., 2001; Lu et al., 2005; Liu et al., 2008; Al-Azemi et al., 2010). Disorders caused by the long-term intake of cadmium in the body are determined by the level of accumulation of Cd2+ in cells and the manifestation of cumulative toxicity. The vulnerability of cells to cadmium largely depends on the level of expression of the genes of metal-binding proteins, metalothioneone, in them. (Antonio et al., 1998; Pavan Kumar and Prasad, 2004; El-Shahat et al., 2009). Cadmium poisoning occurs when it enters the stomach or by inhalation. Absorbed cadmium accumulates in the liver and kidneys as a complex with metalthionein. That is why our researches were aimed at studying the effect of cadmium on the protein synthesis function and the functional state of the liver of laying hens.
Materials and Methods
For the experiment we selected 24 Hisex white class hens at the age of 78 weeks, from which three groups were formed: the control group and two research groups. Groups were formed on the principle of analogs (age and live weight). Chickens from different groups were labeled with persistent organic dyes. The chickens of the control group were on a normal diet, they were fed
with mixed feed and watered without making cadmium sulfate. Within 30 days, cadmium sulfate was added to the drinking water of chickens from the experimental groups in doses: the first group - 2 mg/kg, the second group - 4 mg/kg body weight. Conditions and parameters of the indoor microclimate for all groups of birds were similar. During the experiment we took into account the amount of food consumed and water.
All experimental interventions and slaughter of animals were carried out in compliance with the requirements of the European Convention for the Protection of Vertebrate Animals used for Experimental and Scientific Purposes (Strasbourg, 1985) and the decisions of the First National Congress on Bioethics (Kiev, 2001).
Blood from laying hens was taken from the axillary veins in the periods: before the start of the cadmium sulfate task and the first, seventh, fourteenth, twenty-first and thirieth days of the experiment. Investigated the concentration of protein, its fractions, the activity of aminotransferases by the method (Vlizlo, 2012).
Analysis of the research results was performed using the software package Statistica 6.0. The probability of differences was assessed by Student's t-test. The results of the mean values were considered statistically significant at * - P<0.05, ** - P<0.01, ***- P<0.001 (ANOVA).
Results and Discussion
Enzymes are biological catalysts that accelerate metabolic processes in cell cytoplasm. They are continuously synthesized in cells and penetrate the bloodstream. For enzymes inherent the localization in the corresponding cells and their structural elements (cytoplasm, mitochondria, nuclei). When cell membranes are permeable, enzymes enter the bloodstream and create increased activity in the blood. The study of the activity of enzymes in serum is great diagnostic and prognostic value in various pathological conditions of the structure of parenchymal organs. In the presence of a pathological process in the blood of mammals and birds changes in the qualitative and quantitative composition of enzymes that reflect the intensity and direction of the development of pathology.
The results of studies of the activity of aminotransferases in the serum of laying hens under cadmium loading are shown in table 1. It was found that the activity of alanine aminotransferase in the serum of chickens of the first experimental group has probably increased since the 7th day of the experiment by 13.3%. For the 14th day of the experiment, the activity of the enzyme continued to increase. And on the 14th day of the experiment fluctuated within 0.38 ± 0.007 mmol/g/l, whereas in the control group of laying hens, this indicator was much lower. On the 21st day of the experiment, the activity of alanine aminotransferase in the serum of the birds of the first experimental group was the highest, where compared to the control it increased by 29%.
During the feeding for chickens of the second experimental group of cadmium sulfate at a dose of 4.0 mg/kg body weight, similar increases in the activity of this enzyme were found throughout the experiment. Thus, for the 7th day of the experiment the enzyme activity increased by 20%, whereas on the 14th day - by 21.2%, respectively. The highest activity of alanine aminotransferase was on the 21st day of the experiment, where, respectively, it was 0.45 ± 0.009 mmol/g/l. This increase in enzyme activity is due to an increase in the permeability of hepatocyte cell membranes and mitochondrial membranes and the intracellular enzymes flow into the bloodstream.
Table 1. The activity of aminotransferase serum of laying hens for cadmium loading (M ± m, n=8).
Experimental groups
Before drinking
Days of research
C E1 E2
C E1 E2
0.31 ± 0.01 0.32 ± 0.01 0.30 ± 0.01
4.27 ± 0.18 4.31 ± 0.13
4.28 ± 0.17
7
14
ALT, mmol / g / l 0.30 ± 0.01 0.33 ± 0.01
0.34 ± 0.01* 0.38 ± 0.01*** 0.36 ± 0.01** 0.40 ± 0.01*** AST, mmol/g/l
4.30 ± 0.18 4.49 ± 0.15 4.60 ± 0.19
4.29 ± 0.17 4.82 ± 0.20 5.16 ± 0.23**
21 30
0.31 ± 0.01 0.30 ± 0.01
0.40 ± 0.01*** 0.38 ± 0.01***
0.45 ± 0.01*** 0.43 ± 0.01***
4.35 ± 0.15 4.33 ± 0.13
5.14 ± 0.19** 4.93 ± 0.24*
5.70 ± 0.21*** 5.54 ± 0.25***
In the study of aspartate aminotransferase activity it was found that under cadmium loading the activity of this enzyme increased significantly in the first experimental group at 21 and 30 days of the experiment, whereas in the second experimental group respectively - at 14, 21 and 30 days of the experiment. Thus, the activity of aspartate aminotransferase in the serum of the first and second experimental group of laying hens increased by 12.4 and 20.3%, respectively, relative to the indicators of the control group. During the 21 days of the experiment, enzyme activity increased slightly compared to the previous day. It was the highest in the serum of the second experimental group of laying hens, where it increased by 31% relative to control, while in the first experimental group - by 18.2%. At 30 days of the experiment, the activity of aspartate aminotransferase in the serum of both study groups remained significantly higher than the control.
We think that aminotransferase hyperenzymemia in the serum of diseased animals results from the action of cadmium on the liver, which has a destructive effect on cell membrane phospholipids. This causes an increase in their permeability and release of aminotransferases from hepatocytes into the bloodstream.
Protein is considered the main element, due to which the internal process of "building" in the body. It maintains the fluidity of blood, its viscosity. The protein determines the volume of blood needed in the bloodstream. At the expense of proteins, the shaped elements are kept in a suspended state, and also the transportation of the most important exogenous and endogenous substances is carried out. Thanks to proteins, the pH of the blood is regulated. Protein takes an active part in immune reactions (Hariv & Gutyj, 2016).
It was found that the cadmium load in the blood of laying hens is inhibited by the protein synthesis function of the liver, as indicated by the low level of total protein in their blood. Thus, in the blood of chickens of the first experimental group on the 14th day of the experiment revealed a decrease in the level of total protein by 6.7% relative to control. On the 21st day of the experiment, the test indicator decreased and was 42.11 ± 1.25 g/l, respectively, whereas in the control group it fluctuated within 4673 ± 095 g/l (Table 2).
During bird feeding of cadmium sulphate at a dose of 4,0 mg/kg body weight, a significant decrease in total protein was observed from the 7th day of the experiment, where it decreased by 42% compared to the control group of chickens. At 14 and 21 days of the experiment revealed a decrease in the level of total protein by 9 and 165%. At the 30th day of the experiment, the level of total protein fluctuated within 3965 ± 111 g/l, which was 15% higher than the values of the control group of chickens in the specified period of studies.
The decrease in total protein in the blood of cadmium-loaded chickens was due to a decrease in the albumin fraction. It was found that in the 14th day of the experiment the level of albumin in the blood of the first experimental group decreased by 2.6%, and in the second experimental group - by 4.1% relative to the control group of laying hens. On the 21st day of the experiment, the level of albumin in the blood of the experimental groups was the lowest, where it was 29.62 ± 0.98% in the blood of the first experimental group of chickens and 28.16 ± 0.75% in the blood of the second experimental group. At the 30th day of the experiment, the level of albumin in the blood of chickens of the first and second experimental group remained low and compared to the control group of chickens it decreased by 2.22 and 4.51% respectively.
Table 2. Total content of protein and its fractions in blood of laying hens forcadmium loading (M ± m, n = 8).
Experimental groups Before drinking Days of research
7 14 21 30
Total protein, g/l
C 46.43 ± 0.95 46.60 ± 0.87 46.52 ± 1.05 46.73 ± 0.95 46.60 ± 0.90
E1 46.71 ± 0.80 45.84 ± 1.20 43.38 ± 1.22* 42.11 ± 1.25** 42.41 ± 1.19*
E2 46.58 ± 1.06 44.62 ± 0.96 42.31 ± 1.15* Albumins,% 39.04 ± 1.23*** 39.65 ± 1.11**
C 33.59 ± 0.85 33.92 ± 0.90 33.74 ± 0.70 34.10 ± 0.79 33.85 ± 0.94
E1 34.12 ± 0.90 32.38 ± 0.70 31.14 ± 0.85* 29.62 ± 0.98** 31.63 ± 0.92*
E2 33.78 ± 0.93 31.84 ± 0.89 29.62 ± 0.98** Globulins,% 28.16 ± 0.75*** 29.34 ± 0.97**
C 66.41 ± 1.94 66.08 ± 1.57 66.26 ± 1.70 65.90 ± 1.64 66.15 ± 1.80
E1 64.88 ± 1.65 67.62 ± 2.10 68.86 ± 1.95 70.38 ± 2.05* 68.37 ± 2.11
E2 66.22 ± 1.87 68.16 ± 2.35 70.38 ± 1.85 Coefficient A/G 71.84 ± 2.10* 70.66 ± 1.95
C 0.51 0.51 0.51 0.52 0.51
E1 0.53 0.48 0.45 0.42 0.46
E2 0.51 0.47 0.42 0.39 0.41
When examining the globulin fraction in the blood of laying hens, it was found that the globulins in the blood of the second experimental group probably increased on the 7th day of the experiment. On the 14th day of the experiment, the level of albumin probably increased in all study groups. Thus, in the blood of the first experimental group the level of globulins increased to 68.86 ± 1.95%, and in the second - 70.38 ± 1.85%, which in comparison with the control group it increased by 2.6 and 4.1%, respectively. On the 21st day of the experiment, the level of globulins in the blood of the first and second experimental groups of chickens ranged from 70.38 ± 2.05 to 71.84 ± 2.10%, whereas in the control this indicator was 65.90 ± 1.64%. At 30 days of the experiment, this indicator remained high in the blood of both experimental groups of chickens.
In laying hens during the feeding of cadmium sulfate at doses of 2.0 and 4.0 mg/kg body weight, there is an albumin-globulin disproportion. As a result, the value of A/G coefficient at day 14 of the experiment was 0.45 and 0.42 against 0.51 in clinically healthy birds. Such a magnitude of the coefficient undoubtedly indicates the inhibition of the protein synthesizing function of the liver of laying hens. Increased levels of serum globulin in chickens reflect the intensity of inflammatory processes in their body under cadmium load.
Conclusion
In terms of cadmium loading, laying hens suppress protein-synthesizing liver function, which is manifested by a decrease in total blood protein, a decrease in albumin level and an increase in globulin level. Feeding cadmium sulfate with water at a dose of 4 mg/kg body weight was more likely to be accompanied by a decrease in the level of total protein and albumin than that of cadmium sulfate at a dose of 2.0 mg/kg body weight. Drinking cadmium sulphate laying hens at doses of 2.0 and 4.0 mg/kg body weight promoted aminotransferase activity in their blood. The highest activity of alanine and aspartate aminotransferases was on the 21st day of the experiment. These changes indicate a violation of the functional state of the liver of laying hens under cadmium load.
References
Al-Azemi, M., Omu, F.E., Kehinde, E.O., Anim, J.T., Oriowo, M.A., & Omu, A.E. (2010). Lithium protects against toxic effects of cadmium in the rat testes. J. Assist. Reprod. Genet, 27(8), 469-476. doi: https://doi.org/10.1007/s10815-010-9426-3/ Ali, M.M., Murthy, R.C., & Chandra, S.V. (1986). Developmental and longterm neurobehavioral toxicity of low-level in utero Cd exposure in rats. Neurobehavioral Toxicology and Teratology, 8(5), 463-468.
Antonio, M.T., Benito, M.J., Leret, M.L., & Corpas, I. (1998). Gestation administration of cadmium alters the neurotransmitter levels in newborn rat brains. J Appl Toxicol., 18(2), 83-88.
Chumachenko, V.Y., Chumachenko, V.V., & Pavlenko, O.I. (2004). Study of the immune system. Factors that have an influenceon the resistance of animals. Veterinary Medicine of Ukraine, 5, 33-37 (in Ukrainian).
El-Refaiy, A.I., & Eissa, F.I. (2012). Protective effects of ascorbic acid and zinc against cadmium-induced histopathological, histochemical and cytogenetic changes in rats. Comunicata Scientiae, 3(3), 162-180.
El-Shahat, A.E., Gabr, A., Meki, A.R., & Mehana, E.S. (2009). Altered testicular morphology and oxidative stress induced by cadmium in experimental rats and protective effect of simultaneous green tea extract. Int. J. Morphol., 27(3), 757-764. doi:
https://doi.org/10.4067/S0717-95022009000300020
Fregoneze, J.B., Marinho, C.A., Soares, T., Castro, L., Sarmento, C., Cunha, M., Gonzalez, V., Oliveira, P., Nascimento, T., Luz, C.P., Santana, Jr. P., De-Oliveira, I.R., & e-Castro-e-Silva, E. (1997). Lead (Pb2+) and cadmium (Cd2+) inhibit the dipsogenic action of central beta-adrenergic stimulation by isoproterenol. Brazilian Journal of Medical and Biological Research, 30(3), 419-423. doi: https://doi.org/10.1590/S0100-879X1997000300018.
Grushanska, N. (2017). The content of heavy metals in the cow hair of the northern-eastern biogeochemical zone. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 19(73), 154-158. doi: https://doi.org/10.15421/nvlvet7332 Grynevych, N., Sliusarenko, A., Dyman, T., Sliusarenko, S., Gutyj, B., Kukhtyn, M., Hunchak, V. Kushnir, V. (2018). Etiology and histopathological alterations in some body organs of juvenile rainbow trout Oncorhynchus mykiss (Walbaum, 1792) at nitrite poisoning. Ukrainian Journal of Ecology, 8(1), 402-408. doi: https://doi.org/10.15421/2018 228
Gutyj, B. (2015). The influence of hydravite-e, e-selenium on activity of aminotransferases in bulls blood serum by chronic cadmium toxicosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences, 17(2), 43-47. Retrieved from https: //nvlvet.com.ua/index.php/1ournal/article/view/458
Gutyj, B., Grymak, Y., Drach, M., Bilyk, O., Matsjuk, O., Magrelo, N., Zmiya, M., & Katsaraba, O. (2017). The impact of endogenous intoxication on biochemical indicators of blood of pregnant cows. Regulatory Mechanisms in Biosystems, 8(3), 438-443. doi: https://doi.org/10.15421/021768.
Gutyj, B., Hufriy, D., Binkevych, V., Vischur, V., Binkevych, O., & Kurlyak, I. (2015). The changes of biochemical and morphological indices of rats' blood under chronic cadmium toxicosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(3), 120-123. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/531.
Gutyj, B., Martyshchuk, T., Bushueva, I., Semeniv, B., Parchenko, V., Kaplaushenko, A., Magrelo, N., Hirkovyy, A., Musiy, L., & Murska, S. (2017). Morphological and biochemical indicators of blood of rats poisoned by carbon tetrachloride and subject to action of liposomal preparation. Regulatory Mechanisms in Biosystems, 8(2), 304-309. doi: https://doi.org/10.15421/021748. Gutyj, B., Nazaruk, N., Levkivska, A., Shcherbatyj, A., Sobolev, A., Vavrysevych, J., Hachak, Y., Bilyk, O., Vishchur, V., & Guta, Z. (2017). The influence of nitrate and cadmium load on protein and nitric metabolism in young cattle. Ukrainian Journal of Ecology, 7(2), 9-13
Gutyj, B., Stybel, V., Darmohray, L., Lavryshyn, Y., Turko, I., Hachak, Y., Shcherbatyy, A., Bushueva, I., Parchenko, V., Kaplaushenko, A., & Krushelnytska, O. (2017). Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load. Ukrainian Journal of Ecology, 7(4), 589-596.
Gutyj, B.V. (2015). The activity of antioxidant protecting of the bulls for acute cadmium toxicosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(1), 31-36. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/214. Gutyj, B.V., Binkevych, V., & Binkevych, O. (2016). Hematological changes of rats after cadmium toxicosis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 18(1), 165-167. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/110.
Gutyj, B.V., Gufrij, D., Binkevych, V., Binkevych, O., Kurlyak, I., & Sobolta, A. (2015). Influence of Mevesel & E-selenium on level of intermediate and final products of lipid peroxidation in bulls' blood after cadmium loading. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(1), 190-194. Retrieved from https://nvlvet.com.ua/index.php/journal/article/view/292/. Gutyj, B.V., Murs'ka, S.D., Gufrij, D.F., Hariv, I.I., Levkivs'ka, N.D., Nazaruk, N.V., Gajdjuk, M.B., Pryjma, O.B., Bilyk, O.Ja., & Guta, Z.A. (2016). Influence of cadmium loading on the state of the antioxidant system in the organism of bulls. Visnyk of Dnipropetrovsk University. Biology, ecology, 24(1), 96-102. doi: https://doi.org/10.15421/011611
Gutyj, B.V., Ostapyuk, A.Y., Sobolev, O.I., Vishchur, V.J., Gubash, O.P., Kurtyak, B.M, Kovalskyi, Y.V., Darmohray, L.M., Hunchak, A.V., Tsisaryk, O.Y., Shcherbatyy, A.R., Farionik, T.V., Savchuk, L.B., Palyadichuk, O.R., & Hrymak, K. (2019). Cadmium burden impact on morphological and biochemical blood indicators of poultry. Ukrainian Journal of Ecology, 9(1), 236-239 Hariv, M.I., & Gutyj, B.V. (2016). Vplyv liposomalnoho preparatu Butaintervit na proteinsyntezuvalnu funktsiiu pechinky shchuriv za otruiennia tetrakhlormetanom [Influence of the liposomal preparation Butaintervite on protein synthesis function in the livers of rats under the influence of carbon tetrachloride poisoning]. Visnyk of Dnipropetrovsk University. Biology, medicine, 7(2), 123-126. doi: https://doi.org/10.15421/021622 (in Ukrainian).
Hradovych, N., Paranyak, R., & Zabytivskyi, Y. (2016). Influence of zeolites on lead and cadmium content in separate links of trophic chain in hydroecosystems. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 18, 2(67), 61-65. doi: https://doi.org/10.15421/nvlvet6714.
Hutyi, B.V. (2013). Riven pokaznykiv nefermentnoi systemy antyoksydantnoho zakhystu orhanizmu bychkiv za umov kadmiievoho navantazhennia. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 1(4), 40-45. Retrieved from http://nbuv.gov.ua/UJRN/nvlnu 2013 15 1(4) 10/ (in Ukrainian).
Hutyi, B.V. (2013). Vmist vitaminiv A i E u krovi bychkiv za umov kadmiievoi intoksykatsii. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Veterynarna medytsyna, 2, 31-33. Retrieved from http://nbuv.gov.ua/UJRN/Vsna vet 2013 2 10/ (in Ukrainian).
Hutyi, B.V. (2013). Vplyv E-selenu na aktyvnist hlutationovoi systemy antyoksydantnoho zakhystu orhanizmu buhaitsiv pry kadmiievomu navantazhenni. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Veterynarna medytsyna. 9, 70-73. Retrieved from http://nbuv.gov.ua/UJRN/Vsna vet 2013 9 22/ (in Ukrainian).
Hutyi, B.V. (2013). Vplyv E-selenu na vmist vitaminiv A i E u krovi bychkiv za umov kadmiievoi intoksykatsii. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 3(3), 311-314. Retrieved from http://nbuv.gov.ua/UJRN/nvlnu 2013 15 3(3) 55/ (in Ukrainian).
Hutyi, B.V. (2013). Vplyv khlorydu kadmiiu u riznykh dozakh na aktyvnist aminotransferaz syrovatky krovi buhaitsiv. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 1(1), 49-52. Retrieved from http://nbuv.gov.ua/UJRN/nvlnu 2013 15 1(1) 11/ (in Ukrainian).
Hutyi, B.V. (2013). Vplyv khlorydu kadmiiu u toksychnykh dozakh na hlutationovu systemu antyoksydantnoho zakhystu orhanizmu bychkiv. Veterynarna biotekhnolohiia, 22, 112-116. Retrieved from http://nbuv.gov.ua/UJRN/vbtb 2013 22 23/ (in Ukrainian). Hutyi, B.V. (2013). Vplyv Meveselu na pokaznyky neenzymnoi systemy antyoksydantnoho zakhystu orhanizmu buhaitsiv za umov kadmiievoho navantazhennia. Biolohiia tvaryn, 15(3), 16-21. Retrieved from http://nbuv.gov.ua/UJRN/bitv 2013 15 3 4/ (in Ukrainian).
Hutyi, B.V. (2013). Vplyv meveselu na vmist vitaminiv A i E u krovi bychkiv za umov kadmiievoi intoksykatsii. Naukovyi visnyk Lvivskoho natsionalnoho universytetu veterynarnoi medytsyny ta biotekhnolohii im. Gzhytskoho, 15, 3(1), 78-82. Retrieved from http://nbuv.gov.ua/UJRN/nvlnu 2013 15 3(1) 18/ (in Ukrainian).
Hutyi, B.V. (2013). Vplyv meveselu ta E-selenu na riven pokaznykiv ne fermentnoi systemy antyoksydantnoho zakhystu orhanizmu buhaitsiv pry kadmiievomu navantazhenni. Veterynarna medytsyna, 97, 419-421. Retrieved from http://nbuv.gov.ua/UJRN/vetmed 2013 97 172/ (in Ukrainian).
Khariv, M., Gutyj, B., Butsyak, V., & Khariv, I. (2016). Hematological indices of rat organisms under conditions of oxidative stress and liposomal preparation action. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University. 6 (1), 276-289. doi: http://dx.doi.org/10.15421/201615.
Liu, J., Qian, S.Y., Guo, Q., Jiang, J., Waalkes, M.P., Mason, R.P., & Kadiiska, M.B. (2008). Cadmium generates reactive oxygen- and carbon-centered radicalspecies in rats: Insights from in vivo spin-trappingstudies. Free Radic Biol Med., 45(4), 475-481. doi: https://doi.org/10.1016/j.freeradbiomed.2008.04.041
Lu, J., Jin, T., Nordberg, G., & Nordberg, M. (2005). Metallothionein gene expression in peripheral lymphocytes and renal dysfunction in a population environmentally exposed to cadmium. Toxicol Appl Pharmacol 206(2), 150-156. doi: https://doi.org/10.1016/Ltaap.2004.12.015.
Nazaruk, N., Gutyj, B.V., & Hufriy, D. (2015). Influence of metifen and vitamix se on the activity of aminotransferases of bulls blood serum at cadmium nitrate loading. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 17(1), 121-126. Retrieved from https: //nvlvet.com.ua/index.php/journal/article/view/231/.
Ostapyuk, A.Y., & Gutyj, B.V. (2018). Influence of cadmium loading on morphological parameters of blood of the Laying Hens. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies, 20(88), 48-52. doi: https://doi.org/10.32718/nvlvet8808.
Pavan Kumar, G., & Prasad, M.N.V. (2004). Cadmium-Inducible Proteins in Ceratophyllum demersum L. (a Fresh Water Macrophyte): Toxicity Bioassays and Relevance to Cadmium Detoxification. Bulletin of Environmental Contamination and Toxicology, 73(1), 174-181. doi: https://doi.org/10.1007/s00128-004-0410-4
Peng, L., Wang, X., Huo, X., Xu, X., Lin, K., Zhang, J., Huang, Y., & Wu, K. (2015). Blood cadmium burden and the risk of nasopharyngeal carcinoma: a case-control study in Chinese Chaoshan population. Environmental Science and Pollution Research, 22(16), 12323-12331. doi: https://doi.org/10.1007/s11356-015-4533-4.
Rodriguez, E.M., Bigi, R., Medesani, D.A., Stella, V.S., Greco, L.S.L., Moreno, P.A.R., Monserrat, J.M., Pellerano, G.N., & Ansaldo, M. (2001). Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity. Brazilian Journal of Medical and Biological Research. 34(4), 509-518.
Sachko, R.G., Lesyk, Ja.V., Luchka, I.V., & Nevostruyeva, I.V. (2016). Contents of heavy metals in food, organism and animal products in the Zacarpathian biogeochemical province. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18, 3(71), 8790. doi: https://doi.org/10.15421/nvlvet7120.
Salvatori, F., Talassi, CB, Salzgeber, S.A., Sipinosa, H.S., & Bernardi, M.M. (2004). Embryotoxic and long-term effects of cadmium exposure during embryogenesis in rats. Neurotoxicology and Teratology, 26(5), 673-680. doi: https://doi.org/10.1016/Lntt.2004.05.001
Uetani, M., Kobayashi, E., Suwazono, Y., Okubo, Y., Honda, R., Kido, T., & Nogawa, K. (2005). Selenium, Cadmium, Zinc, Copper, and Iron Concentrations in Heart and Aorta of Patients Exposed to Environmental Cadmium. Bulletin of Environmental Contamination and Toxicology, 75(2), 246-250. doi: https: //doi.org/10.1007/s00128-005-0744-6.
Vlizlo, V.V. (2012). Laboratorni metody doslidzhen u biolohiyi, tvarynnytstvi ta veterynarniy medytsyni [Laboratory methods of investigation in biology, stock-breeding and veterinary]. Spolom, Lviv (in Ukrainian).
Citation:
Gutyj, B., Ostapiuk, A., Kachmar, N., Stadnytska, O., Sobolev, O., Binksevych, V., Petryshak, R., Petryshak, O., Kulyaba, O., Naumyuk, A., Nedashkivsky, V., Nedashkivska, N., Magrelo, N., Golodyuk, I., Nazaruk, N., Binkevych, O. (2019). The effect of cadmium loading on protein synthesis function and functional state of laying hens' liver. Ukrainian Journal of Ecology, 9(3), 222-226. | ("OE^^^MI This work is licensed under a Creative Commons Attribution 4.0. License
