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3The diagnostic capabilities of the optical spectroscopy of blood
serum after freezing
P. Nurgalieva1*, B. Yakimov12, E. Tokar3, O. Parashchuk1, M. Varentsov4, S. Aripshev4, D. Paraschuk1, I. Bratchenko5, N. Sorokin4, O. Cherkasova67, A. Kamalov4, E. Shirshin12**
1- Faculty ofPhysics, M.V. Lomonosov Moscow State University, 1-2 Leninskie Gory, Moscow, Russia 2- Laboratory of Clinical Biophotonics, Sechenov First Moscow State Medical University, Moscow, Russia
3- LLC "Medeum", Moscow, Russia 4- Department of Urology, Medical Research and Education Center, Lomonosov Moscow State University,
Moscow, Russia
5- Department of Laser and Biotechnical Systems, Samara University, Samara, Russia 6- Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, Novosibirsk,
Russia
7- National Research Centre "Kurchatov Institute ", Moscow, Russia * [email protected], ** [email protected]
The non-invasive and rapid measurement capabilities of optical spectroscopy make it an attractive choice for biomedical applications. In a variety of clinical settings, fluorescence and Raman spectroscopy of blood serum have great potential as diagnostic tools [1-3]. The first step of blood serum optical spectroscopy and biochemistry analysis is a pre-analytical stage, namely, sample preparation step. The pre-analytical stage is of high importance, as it may affect the subsequent results. In case of blood serum optical spectroscopy, the pre-analytical stage includes blood collection into serum separation tube by venipuncture, tube centrifugation for serum preparation and freeze-thaw cycle. The freeze-thaw cycle is not essential, but it is commonly employed because it can simplify the research process and facilitate its translation in clinical settings. Although many studies have demonstrated the potential of blood serum optical spectroscopy in biomedical diagnostics, there is still a lack of reports on the optical properties of fresh frozen blood serum. This gap poses a challenge for the practical use of optical spectroscopy of blood serum in real clinical settings.
The objective of this study was to examine the impact of freezing on the optical properties and diagnostic potential of blood serum. In this work, we assessed the effect of blood serum storage at -20°C for 24 hours on its optical spectroscopy signal, namely on autofluorescence, regular Raman spectroscopy and surface-enhanced Raman spectroscopy (SERS) signal.
The work of P.K. Nurgalieva was supported by the PhD student grant by the Fund of Theoretical Physics and Mathematics Development «BASIS» (project No 21-2-9-47-1).
[1] A.V. Gayer, et al, Multifarious analytical capabilities of the UV/Vis protein fluorescence in blood plasma, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 286, 122028-122036, (2023).
[2] J.L. Pichardo-Molina, et al, Raman spectroscopy and multivariate analysis of serum samples from breast cancer patients, Lasers in medical science, 22, 229-236, (2007).
[3] S.Z. Al-Sammarraie, et al, Human blood plasma SERS analysis using silver nanoparticles for cardiovascular diseases detection, Journal
of Biomedical Photonics & Engineering, 10(1), 4-12, (2024).
