CC BY
14THz-I-13
Sub-wavelength-resolution terahertz imaging of soft biological tissues
K. Zaytsev1, N. Chernomyrdin1,I. Dolganova2, G. Katyba2,I. Spektor1, V. Karasik3,I. Reshetov4, V. Tuchin5
1Prokhorov General Physics Institute of the Russian Academy of Sciences, Laboratory of Submillimeter Dielectric Spectroscopy, Moscow, Russian Federation 2Insitute of Solid State Physics of the Russian Academy of Sciences, Laboratory of Shaped Crystals, Chernogolovka, Russian Federation
3Bauman Moscow State Technical University, Research and Educational Center of Photonics, Moscow, Russian Federation
4Sechenov First Moscow State Medical University, Department if Plastic Surgery, Moscow, Russian Federation
5Saratov State University, Department of Optics and Biophotonics, Saratov, Russian Federation
Terahertz (THz) biophotonics attracts considerable interest as a promising tool for diagnosis of malignancies with different nosology and localization [1]. Nevertheless, majority of modern THz spectroscopy and imaging modalities rely on lens- and mirror-based optical systems, which obey the Abbe diffraction limit and provide the spatial resolution of >X; X is electromagnetic wavelength [2]. The resolution of several hundreds of microns, or even of several millimeters, strongly limits capabilities of THz technology in malignancy diagnosis, pushing further developments into the realm of sub-wavelength-resolution THz imaging [1]. In our work, we developed a method of THz solid immersion microscopy for continuous-wave reflection-mode imaging of soft biological tissues with a sub-wavelength spatial resolution [3-5]. In order to achieve strong reduction in the dimensions of beam caustic, an electromagnetic wave is focused into the evanescent field volume behind a medium with a high refractive index. We have experimentally demonstrated a 0.15X-resolution of the proposed imaging modality at X=500 p,m. The proposed technique does not involve any sub-wavelength near-field probes and diaphragms, thus, providing high energy throughout. We have applied the developed method for the THz imaging of various soft biological tissues: a plant leaf blade, cell spheroids, tissues of the breast ex vivo, human brain gliomas ex vivo and glioma models from rats ex vivo [4-6]. The observed results justify capabilities of the proposed THz imaging modality in biology and medicine.
This work was supported by the Russian Science Foundation, Project # 17-79-20346.
References
[1] O.A. Smolyanskaya et al., Progress in Quantum Electronics 62, 1-77 (2018).
[2] N.V. Chernomyrdin et al., Review of Scientific Instruments 88(1), 014703 (2017).
[3] N.V. Chernomyrdin et al., Applied Physics Letters 110(22), 221109 (2017).
[4] N.V. Chernomyrdin et al., Applied Physics Letters 113(h), 111102 (2018).
[5] N.V. Chernomyrdin et al., Optics & Spectroscopy 126(5), 644-651 (2019).
[6] A.A. Gavdush et al., Journal of Biomedical Optics 24(2), 027001 (2019).
