CC BY
14
13. Rugge M, Genta RM. Staging and grading of chronic gastritis. Human Pathology. 2005; 36: 228-233.
14. van Lier MG, Westerman AM, Wagner A. High cancer risk and increased mortality in patients with Peutz-Jegers syndrom. Gut. 2011; 60:141-147.
15. Wang J, Xu L, Shi R. Gastric atrophy and intestinal metaplasia before and after Helicobacter pylori eradication: a meta-analysis. Digestion. 2011; 83:253-260.
|>i'|>:i i и
РОЛЬ ХРОН1ЧНОГО ГАСТРИТУ СЕРЕД ПЕРЕДРАКОВИХ ЗАХВОРЮВАНЬ ШЛУНКА Харченко О.В., Черно В. С., Харченко Н.В., Макаренко П.М., Денисовець 1.В., Денисовець Т.М., Мироненко С.Г.
Метою дослщження е статистичний аналiз стану слизово! шлунка, враженого Helicobacter pylori, у молодих людей, яю займаються спортом. Проведет дослщження хротчного гастриту типу В у студенив-добровольщв, яю займаються спортом. У 92% хротчт гастрити були Helicobacter pylori-асоцшоват. Мiж ступенем обЫметння слизово! оболонки Helicobacter pylori i ступенем лейкоцитарно! шфшьтрацп слизово! оболонки коефiцiент кореляцп Пiрсона rxy =0,935, тiснота зв'язку -дуже сильна, коефiцiент детермiнацi!' D=rxy2-0.874, критичне значення коефiцiента кореляцп з вiрогiднiстю 0,950,2732, критичне значення коефщента кореляцп з вiрогiднiстю 0,99-0,3511, порiвнюючи коефiцiент кореляцй rxy з критичним значенням rcr для значущост 0.95 -rxy >rcr, порiвнюючи коефiцiент кореляции rxy з критичним значенням rcr для значущост 0,99 - rxy>rCT. до коефщента коварiацi! становить 521,641, це дозволяе зробити висновок про статистично значущу залежтсть з iмовiрнiстю 0,99.
Таким чином, хротчний атрофiчний гастрит, асоцтований з Helicobacter pylori, е поширеним захворюванням молодих людей, що займаються спортом, i е центральним серед передракових захворювань шлунку.
Ключовi слова: Helicobacter pylori, гастрит, лейкоцитарна тфшьтращя, слизова оболонка шлунка.
Стаття надтшла 30.09.2019 р.
РОЛЬ ХРОНИЧЕСКОГО ГАСТРИТА СРЕДИ
ПРЕДРАКОВЫХ ЗАБОЛЕВАНИЙ ЖЕЛУДКА Харченко А.В., Черно В.С., Харченко Н.В., Макаренко П.Н., Денисовец И.В., Денисовец Т.М., Мироненко С.Г.
Целью работы является статистический анализ состояния слизистой оболочки желудка пораженной Helicobacter pylori у молодых людей, которые занимаются спортом. Проведены исследования хронического гастрита типа В у студентов-добровольцев, которые занимаются спортом. В 92% хронические гастриты были Helicobacter pylori-асоциированные. Между степенью обсеменения слизистой оболонки Helicobacter pylori i степенью лейкоцитарной инфильтрации слизистой оболочки коэфициент корреляции Пирсона
rxy=0,935, плотность связи-очень сильная, коэфициент детерминации D=rxy2-0.874, критическое значение коефициента корреляции с вероятностью 0,95-0,2732, критическое значение коефициента корреляции с вероятностью 0,99-0,3511, сравнивая коефициент кореляции rxy с критическим значением rcr для значимости 0.95-rxy >rcr, сравнивая коефициент кореляции rxy с критическим значением rcr для значимости 0,99 - rxy>rcr. к коефициенту ковариации составляет 521,641, это позволяет сделать вывод о существовании статистически значимой зависимости с вероятностью 0,99.
Таким образом хронический атрофический Helicobacter pylori-асоциированный гастрит является распространённым заболеванием людей в молодом возрасте, которые занимаются спортом, и занимает центральное место среди предопухолевых заболеваний желудка.
Ключевые слова: Helicobacter pylori, гастрит, лейкоцитарная инфильтрация, слизистая оболочка желудка.
Рецензент Старченко I.I.
DOI 10.26724/2079-8334-2020-3-73-242-247
UDC 616.314.17-008.1-02.001.57:(615.356+546.27):599.323.4
S.A. Schnaider, M.I. Baieiga1, Ye.V. /onihur1, Ye.l. Semeiiov, Ye.K. Tkachenko SE " I he Institute of Siomaioioiy And Maxillofacial Suiiiery of the N AMS of I kiaine", Odesa 'I zhgorod National Medical I niversiiy, I /liiioiod
STUDY ON THE EFFECT OF THE VITAMIN AND MINERAL COMPLEX CONTAINING ZINC L-ASPARTATE ON THE PERIODONTAL CONDITION OF RATS IN THE PRESENCE
OF PERIODONTITIS MODELING
e-mail: ksenianikolainko@gmail.com
The purpose of the study was to study the effect of the vitamin and mineral complex containing zinc L-aspartate on the state of the periodontal tissues of rats under conditions of modeling periodontitis using exogenous collagenase. The vitamin and mineral complex containing zinc L-aspartate had a positive effect, to a greater extent, on the periodontal bone tissue. The complex has shown periodontal protection, anti-inflammatory, antioxidant properties.
Key words: zinc L-aspartate, periodontitis modeling, collagenase, collagen, glycosaminoglycans, gums, periodontal bone tissue, rats.
The study is a fragment of the research project "The effect of hypoxia on the processes of collagen formation and mineralization in models of dental pathology and correction of these disorders ", state registration No. 0118U006963.
Extracellular matrix (ECM) of the connective tissue is defined by a complex system formed by multicellular structural macromolecules: proteoglycans, collagens, and elastins, which maintain its structural integrity. ECM consists of three essential components - a gelling medium, collagen and elastin fibers, and provides a rapid diffusion of substances and "construction" materials between blood and cells.
© S.A. Schnaider, M.I. Balega, 2020
Changes in the state of the extracellular matrix (ECM) of periodontal tissues during periodontitis are carried out using matrix metalloproteinase (MMPs) or collagenases, the main differences of which from other endopeptidases are associated with their ability to destroy the ECM structures of connective tissue, as well as with their dependence on metal ions. The balance between degradation and synthesis of ECM determines the state of periodontal soft tissues and bone tissues during periodontitis.
Zinc is essential in the body for cell growth, protein production, and wound healing. It helps to stabilize blood sugar levels, has a positive effect on the body's immune system, and exhibits antioxidant properties. Zinc is a part of more than 80 enzymes: carbonic anhydrase, RNA and DNA polymerases, carboxypeptidases.
Zinc is one of the most important trace elements that is part of many enzyme systems. It regulates the basic metabolic processes, participates in the metabolism of carbohydrates. Zinc is necessary for the functioning of more than 200 metalloenzymes (carbonic anhydrase, carboxypeptidase A, alkaline phosphatase, RNA polymerase, etc.), as well as for the normal structure of nucleic acids, proteins and cell membranes [2]. Zinc promotes cell growth and development, the normal functioning of the immune system and the provision of an immune response [11]. Zinc deficiency causes difficulties in concentration and memory, decreased cellular and humoral immunity, poor wound healing [4].
Zinc is a potent inhibitor of MMPs and helps to improve the absorption of B vitamins. This element is important for the normal development of bone tissue. The accumulation of metals in connective tissue can affect the formation and absorption of extracellular components of ECM. It was found that Zn2+ ions have an inhibitory effect on MMPs-2 and MMPs-9. Studies of the effect of different ions (zinc, tin, copper, mercury) on gum gelatinase (MMPs-2 and MMPs-9) have shown that ZnSO4 is its most potent inhibitor, while CuSO4, HgSO4, and others are less effective [10].
All of the above predetermined the study of the correction of damage to the extracellular matrix of the periodontium as a result of the periodontitis modeling with a complex of substances necessary for the normal functioning of the connective tissue.
The purpose of the study was to study the effect of the vitamin and mineral complex containing zinc L-aspartate on the state of the periodontal tissues of rats under conditions of modeling periodontitis using exogenous collagenase.
Materials and methods. The experiment was carried out on 21 white female breeding rats of the Wistar line. Animals of the 1st group (6 animal units) were intact. In Groups 2 and 3, periodontitis was modeled by injecting exogenous collagenase solution under the gums (from Clostridium histolyticum lyophilisat 2000 E/mg, Merk, Darmstadt (Germany) at a dose of 1 mg/ml in four areas of the jaws three times during the experiment. In the 3rd group, 7 rats were administered per os with the vitamin and mineral complex "Active Zinc" for 1 tablet/0.2 ml 5 times a week in the morning hours for 55 days, against the background of periodontitis modeling.
In addition to zinc L-aspartate, complex "Active Zinc" (TOV "Elit-PHARM", Dnipro, Ukraine) contains components that contribute to the better absorption of the zinc ion. Zinc is absorbed with the help of phosphorus, calcium, manganese, vitamins A, C, D3.
1 tablet (0.250 g) of the complex contains zinc L-aspartate (active zinc - 12.6 mg), manganese aspartate (Mn2+ - 0.05 mg), calcium hydrogen phosphate (phosphorus - 30 mg), vitamin A - 1666 IU, vit. C - 10 mg, vit. D3 - 2.5 mcg. Excipients: lactose, sorbitol, starch, calcium stearate.
At the end of the experiment, the rats were sacrificed by total exsanguination from the vessels of the heart under anesthesia with thiopental (40 mg/kg). All experiments were carried out in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental or other Scientific Purposes (Strasbourg, 1986). After separating the gums, the jaws were dissected out and the resorption of the alveolar bone was assessed morphometrically. The objects of biochemical studies were the gums and the alveolar bone of rats.
The ECM state of the connective tissue was determined by the levels of hydroxyproline (state of collagen) and glycosaminoglycans (GAGs). The lipid peroxidation level (LPL) was assessed by the content of malondialdehyde (MDA). The activity of antioxidant enzymes catalase [1], and glutathione peroxidase (GPx) was determined. To assess the state of rats' tissues, biochemical parameters were determined by unified methods using commercial reagent kits: alkaline phosphatase (ALP) activity, calcium (Ca2+), phosphorus, Mg2+, zinc, sialic acids. All kits were manufactured by DAC-SpectroMed, Moldova.
The experimental results were processed by conventional statistical methods with the determination of t-criteria for the reliability of differences according to Student's t-test.
Results of the study and their discussion. The study of the complex containing zinc L-aspartate effect on the periodontal tissues of rats was carried out under conditions of periodontitis modeling by subgingival administration of exogenous collagenase at a dose of 1 mg/ml. Oral administration of the
complex against the background of reproduced experimental periodontitis had a positive effect on the state of collagen in rats' periodontal tissues. Thus, under the influence of the complex, the free hydroxyproline level increased by 2.7 times (p = 0.004), the total level increased by 58% (p = 0.003) compared to the group "model of periodontitis", while the bound hydroxyproline level did not change significantly (table 1).
Table 1
Effect of the complex containing zinc L-aspartate on the state of the extracellular matrix (ECM)
of rats' periodontium during the periodontitis modeling (M±m; p)
Studied indices Groups of animals
periodontitis model (M) M+"Active Zn"
Content: GAGs (mg/g) Gum
3.87±0.59 3.55±0.32
hydroxyproline (^mol/g) free 2.12±0.41 5.65±0.87 p=0.004
bound 4.59±0.44 4.94±2.05
total 6.71±0.84 10.6±0.50 p=0.003
Content: GAGs (mg/g) alveolar bone
2.12±0.074 2.56±0.055 p=0.001
hydroxyproline (^mol/g) free 1.41±0.21 2.06±0.00 p=0.011
bound 1.06±0.21 2.47±0.00 p<0.001
total 2.47±0.00 4.77±0.61 p=0.005
Note: In tables 1-3, the significance index p was calculated in comparison with the intact group.
Hydroxyproline content in the periodontal bone tissue also increased significantly: free - by 1.5 times (p = 0.011); bound - by 2.3 times (p<0.001); total - by 93% (p = 0.005). GAGs level in the alveolar bone increased by 21% (p = 0.001; table 1).
Under the influence of a complex containing zinc L-aspartate, the concentration of sialic acids in the blood serum decreased by 22% (p = 0.004; table 2), which indicates both its anti-inflammatory properties in the organism, and the partial restoration of glycoproteins, which indicates an improvement in the ECM state of the connective tissue.
Under the conditions of modeling periodontitis by subgingival administration of collagenases, the alveolar bone resorption in rats increased: in the mandible by 16% (p <0.001) - 41.2±0.88% compared with the intact group: 35.5±0.90%. In the maxilla, the resorption increase was 20% (p=0.016): 31.3±1.43% versus 26.1±1.20% in the intact group (100%).
Table 2
Effect of a complex containing zinc L-aspartate on the content of sialic acids and metals in the periodontium
connective tissue of rats in the modeling of periodontitis (M±m; p)
Studied indices Groups of animals
periodontitis model (M) M+"Active Zn"
blood serum
Content: sialic acids (mmol/ml) 2.40±0.059 1.88±0.096 p=0.004
gum
Content: Zn2+ (^mol/g) 5.96±0.16 7.56±0.074 p<0.001
alveolar bone
Content: Mg2+ (^mol/g) 0.098±0.0095 0.16±0.00 p<0.001
Zn2+ (^mol/g) 2.09±0.28 2.44±0.39
Vitamin and mineral complex "Active Zinc" against the background of periodontitis model significantly reduced the resorption of periodontal bone tissue: by 14% in the mandible (100% in the control group; p = 0.01) and by 6% in the maxilla (p> 0.05; Table 3) compared with data from control groups. Thus, the periodontal protective properties of the complex containing zinc L-aspartate were revealed.
The content of Zn2+ increased in the soft tissues of the periodontium by 21% (p <0.001); in the alveolar bone by 17% (p> 0.05; table 2). At the same time, the content of Mg2+ ions in the periodontal bone tissue significantly increased (by 63%; p <0.001), which indicates an improvement in the state of the ECM in the alveolar bone. It is known that a lack of magnesium leads to a slowdown in protein synthesis. In addition, there was deterioration of the mechanical properties of the gel, which forms the ECM basic substance as a result of the hyaluronidase activation [3].
The results are quite justified in connection with the improvement of the mineral metabolism in this study object - an increase in alkaline phosphatase activity by 56% (p = 0.02) as a result of osteoblast activation, since alkaline phosphatase is their marker enzyme (table 3). The levels of calcium and phosphorus in these conditions increased insignificantly (p> 0.05; table 3).
Table 3
Effect of the complex containing zinc L-aspartate on the state of bone mineral metabolism in rats' periodontium during the periodontitis modeling (M±m; p)
Studied indices Groups of animals
periodontitis model (M) M+"Active Zn"
alveolar bone
Activity: ALP (nmol/s.g.) 0.27±0.035 0.42±0.035 p=0.002
Content: Ca2+ (mmol/g) 0.024±0.0017 0.026±0.0046
Phosphorus (mmol/g) 0.023±0.0035 0.027±0.0052
Periodontal bone resorption indices (%) mandible 41.2±0.88 35.3±1.66 p=0.01
maxilla 31.3±1.43 29.5±1.31
Under the influence of the complex, the level of LPL processes decreased - the MDA content in the gums decreased by 14% (p = 0.02; table 4). At the same time, the activity of the antioxidant enzyme catalase in this study object increased by 28% (p = 0.05). Insufficient functioning of the antioxidant enzyme glutathione peroxidase in the gums was evidenced by a 1.9-fold decrease in its activity (p = 0.003) compared with the "Periodontitis model" group (table 4). In the periodontal bone tissue, the complex, more significantly than in the gums, reduced the level of peroxide products: the MDA content decreased by 2.2 times (p = 0.001), which may indicate the antioxidant properties of the complex containing zinc L-aspartate.
Catalase activity in the periodontal bone tissue under the complex influence changed insignificantly, while the activity of glutathione peroxidase increased by 6% (p = 0.06) compared with the control group (table 4).
Table 4
Effect of a complex containing zinc L-aspartate on the MDA content and the activity of antioxidant enzymes
in the periodontium connective tissue of rats in the modeling of periodontitis (M±m; p)
Studied indices Groups of animals
periodontitis model (M) M+"Active Zn"
Content: MDA (nmol/g) gum
50.3±1.02 43.1±2.51 p=0.02
Activity: catalase (mkat/g) 13.9±1.90 17.8±0.84 p=0.05
GPx (^mol/s.g.) 148±6.38 77.5±7.30 p=0.003
Content: MDA (nmol/g) alveolar bone
0.80±0.083 0.37±0.012 p<0.001
Activity: catalase (mkat/g) 10.4±0.84 10.7±1.61
GPx (^mol/s.g.) 68.9±0.97 73.0±1.68 p=0.06
Studies have shown that the vitamin and mineral complex containing zinc L-aspartate, administered orally to rats against the background of reproduced experimental periodontitis, had a positive effect on the periodontal tissues of rats. First of all, under its influence, the state of collagen, recorded by the level of hydroxyproline (total, free, bound) was improved, which levels significantly increased compared to the control group (periodontitis model). In addition, in hard periodontal tissues, the complex restored the state of the gel, which forms the ECM basis of the connective tissue.
The zinc-containing complex significantly, by 1.6 times, increased the activity of alkaline phosphatase, an enzyme localized in bone tissue on the outer surface of osteoblast membranes. In this regard, the complex against the background of the periodontitis model significantly reduced the resorptive processes in the alveolar bone process of rats.
According to the literature, zinc is one of the components of enzymatic systems, which activity affects the growth, development and physiological state of the organism. It is referred to as trace elements that promote the synthesis of collagen, glycosaminoglycans. It is directly involved in the synthesis of bone matrix [13]. According to a number of scientists [9, 10, 11, 14], zinc increases bone formation, accelerates its mineralization, reduces bone resorption and stimulates the activity of alkaline phosphatase in osteoblast cell culture. Alkaline phosphatase is one of the most representative proteins of osteoblast differentiation as bone markers. As a result of these studies, the authors demonstrated the anabolic role of zinc in the formation of bone tissue under the action of osteoblasts [12, 14].
Zinc stimulates osteoblast proliferation and differentiation, as well as protein synthesis in osteoblasts. The importance of the zinc effect on the bone tissue metabolism is evidenced by information about its deficiency, in which the amount of bone mass decreases [8, 9]. Zinc deficiency contributes to the stress of DNA synthesis and protein metabolism, which leads to disruption of the organic matrix metabolism [1, 11].
Severe metabolic disorders of trace elements, including zinc, occur already in the early stages of the pathological process. Thus, the zinc content in the blood plasma of rats was reduced during the gingivitis and periodontitis reproduction, and more pronounced changes were observed in the group of rats with gingivitis [5].
The molecular mechanisms of zinc deficiency are based on oxidative stress in cells and tissues, as well as an increase in the synthesis of proinflammatory cytokines [7]. Zinc is involved in redox processes. Zinc cations stabilize the permeability of cell membranes and effect as a protector of free radical reactions.
The studied complex, containing zinc L-aspartate showed anti-inflammatory effect at the body level. It reduced the level of sialic acids in the blood serum, since it is known that an increase in the sialic acid content indicates an increase in inflammation in the tissues. It is known that sialic acids, derivatives of neuraminic acid, are formed under the action of neuraminidase during the breakdown of ECM glycoproteins. In our studies, the complex containing zinc L-aspartate showed antioxidant properties. It reduced the level of peroxide processes in the gums and alveolar bone. Under the complex influence, the Zn2+ content in the gums of experimental animals increased by 21%, in the bone tissue of the periodontium - by 17%.
According to the data, the optimal amount of zinc ions in the organism is an important factor that ensures the regenerative function of the connective tissue. During wound healing, zinc provides a stabilizing effect on the cytoplasmic membranes, preventing the hydrolytic enzyme release, such as cathepsin D and collagenase, which control the rate of damaged tissue degradation [6].
Thus, considering the positive results of experimental studies, the zinc content in the complex "Active Zinc" was optimal. The complex can be recommended as a means that promote bone tissue formation, has a direct effect on reducing alveolar bone resorption, and also replenishes the deficiency of the osteotropic trace element zinc, which is involved in vital metabolic processes of the organism.
Conclusions
1. Vitamin and mineral complex containing zinc L-aspartate, administered orally against the background of reproduced experimental periodontitis, increased by 58% the level of total hydroxyproline in the gums; in the alveolar bone - by 93%; the glycosaminoglycans content in the periodontal bone tissue increased by 21%.
2. The complex significantly (by 14%) reduced the alveolar bone resorption, which indicates its periodontal protective properties. It significantly improved mineral metabolism - increased the alkaline phosphatase activity in the periodontal bone by 56%.
3. Under the complex influence, the level of LPL processes in the gums and more significantly in the bone tissue of the periodontium decreased significantly; on the body level, the complex containing zinc L-aspartate reduced the concentration of sialic acids in the blood serum, which indicates its antiinflammatory properties.
4. Vitamin and mineral complexes containing zinc L-aspartate can be recommended for improving
the metabolism of periodontal connective tissue and bone mineral metabolism in periodontitis. References
1. Laricheva OM. Protsesy vilnoradykalnoho perekysnoho okysnennya ta stan antyoksydantnoho zahystu legen shchuriv iz znyzhenoyu aktyvnistyu epifizu. Visnyk problem biolohiyi i medytsyny. 2015; 1: 110-114. [in Ukrainian]
2. Presnyakova MV, Kostina OV. Biologicheskaya rol tsinka i yego znachimost v patogeneze rasstroystv autisticheskogo spektra. Sotsialnaya i klinicheskaya psikhiatriya. 2019; 29(3): 63-70. [in Russian]
3. Torshin IYu, Gromova OA, Rudakov KV. Sistematicheskiy analiz molekulyarnykh mekhanizmov vozdeystviya magniya na displazii soedinitelnoy tkani. Kardiologiya. 2011; 5: 7-11. [in Russian]
4. Sheybak VM. Biologicheskoe znachenie i regulyatsiya gomeostaza tsinka u mlekopitayushchikh. Problemy zdorovya i ekologii. 2016; 4 (50): 11-16. [in Russian]
5. Sheybak LN. Rol i znachenie tsinka v perinatologii. Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta. 2015; 2 (50):30-36.
6. Cheremysina VF. Osoblyvosti obminu midi ta tsinku v peryferychniy krovi shchuriv iz zakhvoryuvannyamy myakikh tkanyn parodontu. Svit medytsyny i biolohiyi. 2017; 13(1): 148-151. [in Ukrainian]
7. Demkovych A. Humoral immune reactivity disorders in experimental parodontitis and their correction by flavonol. Svit medytsyny i biolohiyi. 2017; 3(61): 97-100. http://dx.doi.org/10.26724/ 2079-8334-2017-3-61-97-100
8. Fuhua XU et al. Essential role of ARID2 protein-containing SWI/SNF complex in tissue-specific gene expression. Journal of Biological Chemistry 2012; 287(7): 5033-5041. https://doi.org/10.1074/ jbc.m111.279968
9. Hadley K. Dietary zinc reduces osteoclast resorption activities and increases markers of osteoblast differentiation, matrix maturation, and mineralization in the long bones of growing rats. The Journal of nutritional biochemistry, 2010, 21.4: 297-303. https://doi.org/10.1016/j.jnutbio.2009.01.002
10. Nosrati R, Kheirouri S, Ghodsi R, Ojaghi H. The effects of zinc treatment on matrix metalloproteinases: A systematic review. J Trace Elem Med Biol. 2019; 56: 107-115. https://doi.org/10.1016 /j.jtemb.2019.08.001
11. Prasad AS. Discovery of human zinc deficiency: its impact on human health and disease. Advances in nutrition 2013; 4(2): 176-190. https://doi.org/10.3945/an.112.003210
12. Richard JM. Zinc in the mouth, its interactions with dental enamel and possible effects on caries; a review of the literature. International dental journal, 2011, 61: 46-54. https://doi.org/10.1111/j.1875-595X. 2011.00049.x
13. Sanchez-Ruiz JM. Protein kinetic stability. Biophysical chemistry 2010; 148(1-3): 1-15. https://doi.org/10.1016/j.bpc.2010.02.004
14. Seo Hyun-Ju, et al. Zinc may increase bone formation through stimulating cell proliferation, alkaline phosphatase activity and collagen synthesis in osteoblastic MC3T3-E1 cells. Nutrition research and practice 2010; 4(5): 356-361. https://doi.org/10.4162/nrp.2010.4.5.356
IiU!!!!!!!!^
ВИВЧЕННЯ ВПЛИВУ В1ТАМ1ННО- ИЗУЧЕНИЕ ВЛИЯНИЯ ВИТАМИННО-
М1НЕРАЛЬНОГО КОМПЛЕКСУ, ЩО М1СТИТЬ МИНЕРАЛЬНОГО КОМПЛЕКСА, СОДЕРЖАЩЕГО
ЦИНК L-АСПАРАПНАТ, НА СТАН ПАРОДОНТА ЦИНК L-АСПАРАГИНАТ, НА СОСТОЯНИЕ
ЩУР1В В УМОВАХ МОДЕЛЮВАННЯ ПАРОДОНТА КРЫС В УСЛОВИЯХ
ПАРОДОНТИТУ МОДЕЛИРОВАНИЯ ПАРОДОНТИТА
Шнайдер С.А., Балега М.1., Зомбор С.В., Шнайдер С.А., Балега М.И., Зомбор Е.В.,
Семенов G.I., Ткаченко е.К. Семенов Е.И., Ткаченко Е.К.
Метою дослщження було вивчення впливу Целью исследования явилось изучение влияния
втмшно-мшерального комплексу, що мiстить цинк L- витаминно-минерального комплекса, содержащего цинк L-
аспарагiнат, на стан тканин пародонта щурiв в умовах аспарагинат, на состояние тканей пародонта крыс в условиях
моделювання пародонтиту за допомогою екзогенно! моделирования пародонтита с помощью экзогенной
колагенази. Втмшно-мшеральний комплекс, що мютить коллагеназы. Витаминно-минеральный комплекс,
цинк L-аспарапнат, мало позитивний вплив бшьшою содержащий цинк L-аспарагинат, оказал положительное
мiрою на кiсткову тканину пародонту. Комплекс проявив влияние в большей степени на костную ткань пародонта.
пародонтопротекторш, протизапальш, антиоксидантш Комплекс проявил пародонтопротекторные,
властивостi. противовоспалительные, антиоксидантные свойства.
Ключовi слова: цинк L-аспарапнат, моделювання Ключевые слова: цинк L-аспарагинат, моделирование
пародонтиту, колагеназа, колаген, глкозамшогакани, пародонтита, коллагеназа, коллаген, гликозаминогликаны,
ясна, юсткова тканина пародонту, щури. десна, костная ткань пародонта, крысы.
Стаття надшшла 30.09.2019 р. Рецензент Срошенко Г.А.
DOI 10.26724/2079-8334-2020-3-73-247-252
UDC (577.161.2+577.161.5).001.5:616.314.17-008.1-092.4
S. A. Schn;ii<ler. V. V. I lumeniuk1. V.N. Horokhivsky i. O.V VTremova1.
M.I. Khristova2. Ye.K. Ikachenko SE " T he Institute of Stomatology And Maxillofacial Surgery of the N AMS of I kraine". Oilesji 'Dmiylo Halytsky Lviv National Medical I niversity. Lviv
2Odessa National Medical I niversity. Odesa
EFFECT OF HORMONE-ACTIVE METABOLITES OF CHOLECALCIFEROL ON THE STATE OF THE ORAL CAVITY TISSUES IN RATS UNDER THE CONDITIONS OF ESTROGEN DEFICIENCY AND TRAUMATIC STRESS
e-mail: ksenianikolainko@gmail.com
The purpose of the study was to comparatively establish the effect of active metabolites of vitamin D3 on the periodontal condition of rats under conditions of experimental estrogen deficiency and traumatic stress. The experiment was carried out on 31 female breeding Wistar rats. 1st group was intact one (8 animals). The rats of the 2nd - 4th groups underwent ovariectomy and a fQnQB frUTurD GrQiQ2 wB CQitrQ QiD(8 rQs); rQs Of thD3rd did 4th grQiQ rnTlird QJ Q Q-mjQIQis qgnast thD background of the pathogenic effect: Group 3 (8 rats) - 1-a-hydroxycholecalciferol at a dose of 0.1 ¡ig QJ dmQ_r rBt; H thDGrQiQ
4 (7 rats) - 24,25-hydroxycholecalciferol at a dose of 1.25 ¡ig per day/rat. At the time of sacrifice, the animals were 15 months old. Under the influence of risk factors for periodontitis, protective properties of hormone-active metabolites of vitamin D3 were observed. They were expressed in inhibition of lipid peroxidation processes in the oral mucosa of rats, as well as periodontal protective effects when using 24,25-hydroxycholecalciferol.
Key words: vitamin D3 metabolites, estrogen deficiency, traumatic stress, periodontal protection properties, antioxidant effect, rats.
The study is a fragment of the research project "The effect of hypoxia on the processes of collagen formation and mineralization in models of dental pathology and correction of these disorders", state registration No. 0118U006963.
Vitamin D3 or cholecalciferol, which realizes its action in the organism through active metabolites - 25OHD3, 1,25(OH)2D3 and 24,25(OHhD3, is directly involved in the bone tissue metabolism. 1,25(OHhD3 or calcitriol, is the most biologically active metabolite of vitamin D3. With a deficiency of calcium and phosphorus, the metabolism of 25OHD3 follows the formation of 1,25 (OH) 2D3, which is catalyzed by the enzyme 1-a-hydroxylase, which is present in the mitochondria of renal tubular epithelial cells. With an increased or normal concentration of calcium and phosphorus in the blood serum, an alternative metabolite, 25OHD3 - 24,25(OH)2D3, is formed with 24-hydroxylase [10]. The fundamental
© S.A. Schnaider, V.V. Humeniuk, 2020
