CC BY
3*
ALT'23 The 30th International Conference on Advanced Laser Technologies
B-P-4
Study of in vivo optical clearing of the human oral cavity mucosa by
Raman Spectroscopy
E. N. Lazareva12, V. I. Kochubey12, I. Yu. Yanina1'2, Yu.V. Kistenev2, V. V. Tuchin1'2
1-Institute of Physics and Science Medical Center, Saratov State University (83 Astrakhanskaya st., 410012 Saratov,
Russia)
2- Laboratory of Laser Molecular Imaging and Machine Learning, Tomsk State University (36 Lenin's av., 634050
Tomsk, Russia) lazarevaen@ list.ru
Optical methods are increasingly being used for new scientific developments in medicine due to a number of advantage [1]. One of these methods is Raman spectroscopy, which makes it possible to carry out a quantitative and qualitative chemical analysis and to evaluate changes in the conformation of molecules [1, 2]. However, the use of optical methods, in particular, Raman spectroscopy, for working with biological tissues has limitations, such as, the high power of the radiation used and the low probing depth. To increase the efficiency of these methods, the use of optical clearing can be prospective [3]. This pilot study demonstrates the use of optical clearing technology for the oral mucosa of volunteers in the study using Raman spectroscopy. The effect on tissue of optical clearing agents, such as a solution of 40% glucose and mixtures based on sorbitol, propylene glycol and water, on the tissue was investigated.
For example, the Raman spectra of the oral mucosa from the inner cheek before and after exposure to 40% glucose solutions are shown in Figure 1.
Fig. 1. Raman spectra of an area of the oral mucosa of a volunteer before and after exposure to a 40% glucose solution.
The effect of a 40% glucose solution on the oral mucosa shifted the lipid band from 786 cm-1 to 797 cm-1 and the amides/proteins band from 1661 cm-1 to 1671 cm-1. A significant increase in intensity is observed for glucose at 420 cm-1 and the saccharide bands at 842 cm-1 and 872 cm-1, which may indicate the interaction of proteins with glucose [4]. Glucose peaks at 1071 cm-1 and 857 cm-1 refer to C-O-H deformation stretching and C-C stretching vibrations and, according to the literature data, may indicate the interaction of glucose with tissue proteins and the formation of hydrogen bonds [5, 6].
The study was supported by RSFgrant no. 23-14-00287, https://rscf.ru/project/23-14-00287/.
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