CC BY
5*
ALT'23 The 30th International Conference on Advanced Laser Technologies
N-I-11
Study of biofilms typical for ENT pathologies by THz high resolution
spectroscopy
V.Vaks1,2, A-Ayzenshtadt1'3, V-Anfertev1'2, E.Domracheva1'2, M-Chernyaeva1'2,
P.Mokeeva3, D.Kryazhev4
1-Institute for Physics of Microstructures RAS, 603950, Russia, Nizhny Novgorod, GSP-105 2- Lobachevsky University, 603022, Russia, Nizhny Novgorod, Gagarina av., 23 3- Children's Municipal Clinical Hospital No.1,603081, Russia, Nizhny Novgorod, Gagarina av., 76 4 - Blokhina Scientific Research Institute of Epidemiology and Microbiology of Nizhny Novgorod,603950, Russia,
Nizhny Novgorod, Malaya Yamskaya str., 71
Nowadays spectroscopic analytical methods, operating in the frequency ranges from microwave to ultra-violet as well as other physico-chemical methods (mass-spectrometry, microscopy etc.) are used for medical diagnostics. These methods allow to reveal the metabolites, typical for a specific disease. When compiling a metabolic profile one can diagnose a disease and its etiology, as well as predict treatment. One of the complicated problem is to identify a biofilm, being the main type of existence of bacterial associations, which has its spatial and metabolic structure and forms on the mucosa in a human body. The work is devoted to analyzing the metabolites composition of the biofilms, forming at chronic adenotonsillitis, by high-resolution THz spectroscopy and the comparison of the thermal decomposition products of biofilms formed by Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumoniae and Enterobacter coli.
A THz spectroscopy based on nonstationary effects such as inducing and decaying the free dumping polarization in a gas sample at the interaction of radiation and gas molecules can be considered as a high sensitive method of investigating the multicomponent gas mixtures The devices can be realized in phase switching or fast sweeping modes of the probing radiation. The spectrometers with phase switching or with fast frequency sweeping developed by the authors, operating in the range of 118-178 GHz, were used. The sensitivity of the recorded absorption coefficient for these spectrometers at a cell length of 1 m is from 10-7cm-1 to 5*10-8cm-1. The THz gas spectroscopy method allows to detect volatile compounds in a multicom-ponent gas mixture, including ones of biological origin. Therefore, it is promising to study the volatile compounds (products of thermal decomposition of macromolecules) as secondary metabolites of films, that appear when the sample is heated. The main pathobionts and their ability to biofilm formation at chronic ade-notonsillitis with the identification of tissue metabolites with using the THz high resolution gas spectroscopy methods were studied, at that he influence of the pathogen was analyzed.
The biofilms formed by such bacterial types as Staphylococcus aureus, Streptococcus pyogenes Klebsiella pneumoniae and Enterobacter coli were studied. The compositions of their thermal decomposition products differ significantly. The results obtained can be used in the future to reveal the infection of ENT organs tissues with certain bacteria, as well as to detect the presence of biofilms in the microflora of ENT organs.
This research was funded by the Russian Science Foundation, grant No. 21-19-00357, https://rscf.ru/en/project/21-19-00357/.
