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8Key words: Biejiajian Pill, H22 , JAK/STAT signaling pathway, Survivin References:
1.SUN Yang, WU Bo-yan, CHE Yan-xin, et al. Antitumor Effect of Biejiajian Pill on H22 Tumor Bearing Mice and Study on its Morphology// Lishizhen Medical and Material Research.— 2016. — Vol.27.-№ 4. - C.849-851.
2.WEN Bin, SUN Haitao, HE Songqi, et al. Inhibitory effect of Biejiajian pills on HepG2 cell xenograft growth and expression of P-catenin and Tbx3 in nude mice// Journal of Southern Medical University. — 2016.— Vol.36.-№ 2. - C.210-214.
3.Tamara Zoranovic, Lydia Grmai, and Erika A Bach.Regulation of proliferation, cell competition, and cellular growth by the Dro-sophila JAK-STAT pathway//Landes Bioscience. — 2013.— Vol.2-№ 3. - C.e25408-1-7.
Correspondence to: Associate Professor SUN Yang,Biology Department, School Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin, Heilongjiang 150040, P.R.China.
Email: [email protected]
Acknowledgements: This work has financially supported byHeilongjiang Postdoctoral Fund(No.LBH-Z13205),
China Postdoctoral Science Foundation(No.2014M551288), and Heilongjiang University of Chinese Medicine foundition(-No.051234).
STUDIES ON THE INHIBITORY EFFECTS OF N - CINNAMOYLPITRESCINE IN VISCUMCOLORATUMNAKAI ON HT-29
COLON CANCER CELL
SUN Yong-hui, SUN Nan, LI Jing, ZHAO Jing, ZHANG Jing
(Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China )
Abstract: To study the inhibitory effects of N-cinnamoylpitrescine on the growth of HT-29 colon cancer cells. In vitro cultured HT-29 colon cancer cells were inoculated in 96 well plates. AddingN- cinnamoylpitres-cineafter the cells grew well.N-cinnamoylpitrescinewas divided into control group:0.8mg/mL group, 0.4mg/ mL group, 0.2mg/mL group, 0.1mg/mL group, 0.05mg/mL group. After the intervention of 48h, the inhibitory effects of N-cinnamoylpitrescine on HT-29 colon cancer cells were observed by MTT assay. And calculate the cell inhibition rate.Theabsorbanceof each experimental group with the increase of concentration decreased, tumor inhibition rate increased gradually.The IC50value of N- cinnamoylpitrescinewas 0.499mg/mL.
Key Words : N-cinnamoylpitrescine ; HT-29 Colon cancer ; Inhibition
Colon cancer is a common digestive system malignancy that threatens human's healthandlife.In 2010,theAmeri-can Cancer Society released a statistical report showing that the incidence and mortality rates of colon cancer in both men and women in the United States,ranking second and third, respectively.Mistletoe, as a natural anticancer drug, has been widely used in the treatment of cancer.Adjuvant chemotherapy with mistletoe extractcan significantly improve quality of life and reduce recurrence in cancer patients, and has been shown to significantly inhibit metastasis and recurrence of cancer.In Germany, people who choose the treatment for cancer are increasingat a rate of 20% one year.Experts predict mistletoe is also expected to be a plant source of anticancer drugs after taxol.
Materials
1.1Cell line Human
colon cancer cell line HT-29 was purchased at the Cancer Research Institute of the Provincial Cancer Hospital. 1.2Reagents
RPMI medium, fetalbovineserum, penicillin and streptomycin trypsin, MTT, DMSO. 1.3Instruments
HF90 CO2incubator,automaticenzyme-linked, Olympus CKX41 inverted microscopic,tissue culture flasks, 96 well plates.
Methods
2.1Cell incubation
Human colon cancer cell lines HT-29 were cultured under standard cell
cultureconditions (37°C, 100% relative humidity, 5% CO2)in RPMI medium, supplementedwith 10% heat-inactivated fetal bovine serum, 100 U/mlpenicillin streptomycin. Digest it every 2-3 days with trypsin,After 3-4 days of passage, logarithmic growth phase cells were used for experiments.
Configuration drug
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RPMI medium was used to dilute the drug. According to thepreliminary test results,N-dnnamoylpitresdne was divided into five concentrations: 0.8mg/ml, 0.4mg/ml, 0.2mg/ml, 0.1mg/ml and 0.05mg/ml.And set a control group of 0mg/m.
MTT assay
100 ^l of cell suspension of density 1 x 105 cells/wellwas placed
into each well of 96-well plates and incubated for 24 h. Then, the medium was removed and added the N-cinnam-oylpitrescine to cultured cells in 96 well plates and incubated for 48 h. Added 100 ^L of the tetrazoliumdye (MTT) solution to each well of 96 well platesand incubated for 4 h at 37°C.MTT reagent was discarded and added150 ^L of DMSO. The plate was placed in a plate shaker for 10 min. Then absorbance value (A) was read on anautomatic enzyme-linked at 490 nm. Inhibition rate (%)= (The average absorbance value of the control group — The average absorbance value of the experimental group) / The average absorbance value of the control groupx100%.
Statistical analysis Spss22.0 statistical analysis software is used to process data, the
T test was used in the comparison between the experimental group and the control group, P<0.05 was the significant difference, The IC50 value was calculated according to regression equation.
Results The absorbance ofeach experimental group with the increase of concentration decreased,tumor inhibition rate increased gradually. The inhibitory rates of N-cinnamoylpitrescine on colon cancer HT-29 cells were 10.71% ч 13.25%ч 18.36%ч 38.16%4 71.06%, IC50 value was 0.499mg/ml.
Conclusion The anticancer activity was measured by the MTTmethods. The results showed that N-cinnamoylpitres-cine had anticancer activity. But the mechanism of N-cinnamoylpitrescine treatment is not clear, which will affect the large-scale use and development of the drug. Therefore, the mechanism of N-cinnamoylpitrescine on colon cancer needs to be studied.
FIRE NEEDLE THORN AROUND THE SUPERFICIAL PARTS OF THE BODY IN THE CLINICAL APPLICATION OF THE TREATMENT OF MALIGNANT TUMORS
Suqinsun
The second hospital affiliated to Heilongjiang university of Chinese medicineHarbin15001 E-mailaddress: [email protected]
[Abstract]:This paper introduces our experiencewith fire needle acupuncture in the treatment ofperi human malignant tumor, this method can make the part of the massdisappear, replacing the surgical treatment, and reducing the patient's financial and spiritual burden.
[Keywords]:Fire needle; Fire needlethorn around; malignant tumor; Superficial malignant tumor of the body
Cancer is one of the major diseases that are serious threats to human survival and social development ofand Cancer control has become a health priority for governments around the world.From the national cancer registry of the "2012 China tumor registration report" issued, according to six people are diagnosed with cancer every minute.Located in a shallow body tumors, such as breast cancer, soft tissue tumor, thyroid cancer,theseare the superficial parts malignant tumor.Such tumors, if no distant metastasis, the preferred surgical treatment. Surgical treatment not only to the patient to a heavy financial burden, but also to suffer organ damage.(Surgical treatment will not only be a heavy financial burden to the patient , but also bring damage to the organ.I Division in the treatment of such diseases, in addition to the use of traditional Chinese medicine dialysis medication, at the same time, the application of fire needle acupuncture treatment, can make some tumors shrink or even disappear, replace the surgical treatment, reliable, no side effects.
Method of operation: The patient to take the sitting position or lying position, first determine the tumor surface positioning, do a good job marking, conventional acupuncture parts of skin disinfection, followed by a hand holding alcohol lamp, one hand holding the needle, the needle placed in the fire outside the flame, When quickly penetrate around the lesion, the lesion and normal tissue at the junction of the need for needle. Perimeter method used for the needle with a special fire needle, each needle is about 1cm interval is appropriate, acupuncture depth of the depth of the lesion depending on the depth of the deep needle acupuncture deep lesions shallow needle shallow. Daily or every other day or twice a week.
Example: Liu xx, male, 80 years old, married, December 27, 2016 due to the right lower limb thigh back mass, hard, treatment in Beijing Tongzhou District West Health Center,ultrasound show: Right thigh soft tissue occupancy, consider the possibility of malignant, the size of about 40 * 64 * 111mm, biopsy pathology: right thigh spindle cell tumor, morphological and immunological enzyme tend to malignant or low degree of malignant.Consider the patient frail, no surgery and radiotherapy and chemotherapy.On the 4th of February in our department of traditional Chinese medicine syndrome plus fire needle acupuncture treatment for 14 days, because patients live in the field, home rest to March 7 line of the second fire needle treatment, March 16 local mass disappeared, with Hand touch the lumps, check the color Doppler: right side of the dorsal medial sulcus see 85 * 28 * 70mm hypoechoic, the border is still clear, composition leaves, echo uneven, visible arterial blood flow signal, right thigh dorsal Muscle muscle solid place.
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