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Volga Neuroscience School 2016 Astroglial control of rhythm genesis in the brain
Stimulus Induced Plasticity in Dissociated Neuronal Network with Direct Connectivity
V.N.Kolpakov1'3 *, Y.I. Pigareva1, A.A. Gladkov1,3, E.I. Malishev2, A.Y. Bukatin2, A.S. Pimashkin1, V.B. Kazantsev1, I.V. Mukhina1,3
1 Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, Russia;
2 Saint-Petersburg Academic University - Nanotechnology Research and Education Centre of the RAS, Saint-Petersburg, Russia;
3 Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia. * Presenting e-mail: kolpakov@neuro.nnov.ru
Aims
In this study we used hippocampal neuronal cultures grown on microfludic device which consisted of two chambers with two cultures. The cultures were synaptically coupled by axons grown through microchannels. We used electrical stimulation applied to these pathways to induce synaptic plasticity of the neuronal culture. This method allows to explore a fundamental mechanisms of synaptic plasticity at the network level, and the interaction of cells in the brain.
Methods
Neuronal hippocampal cultures (E18) grown on microstructures (chips) made of PDMS (polydimethylsiloxane) allow to control the growth of neuronal branches with a given direction through the microchannels. The separation of cellular populations allow to determinethe presynaptic and postsynaptic neurons, which is necessary for effective application of electrical stimulation protocols, causing potentiation or depression. For study of synaptic plasticity we observed changes in efficiency of electrical activity propagation between two separate neuronal cultures, connected by axonal branches. To register the electrical activity of the neuronal cultures we used multielectrode arrays (Multichannel systems, Germany) with 59 independent electrodes.
Results
To induce potentiation and depression we used STDP electrical stimulation protocol. The protocol consisted of a series of stimuli at 50 Hz and it was applied to two groups of electrodes in the pre- and postsynaptic neurons. The delay between the stimuli of different groups of the electrodes in stimulation was 50 ms. First we conducted low frequency stimulation (60 pulses at each of seven selected electrodes - 3 presynaptic area, 3 postsynaptic area, one in the microchannel). Then conducted high frequency stimulation (± 800mV, 260 ms per phase 20 stimuli with 100 ms intervals, 150 trials with 6 sec. intervals.
Conclusions
Preliminary results show that network plasticity can be induced in cultured network of by electrical applied to the neurons in the microchannels. Probability of the burst propagation between chambers significantly changed after high frequency stimulation.
Acknowledgements
This research was supported by the Russian Science Foundation (14-19-01381). References
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3. Pelt J., Vajda I., Wolters P. S., Corner M. A. and Ramakers G. J. Dynamics and plasticity in developing neuronal networks in vitro. Prog. Brain Res. 2005. 147, P. 173 -188.
4. Ruthazer E. S. You're perfect, now change - redefining the role of developmental plasticity. Neuron 2005. 45, P. 825 -828.
5. Wagenaar D., Pine J., Potter S.M. Searching for plasticity in dissociated cortical cultures on multi-electrode arrays. J. Negat. Results Biomed. 2006. 5. 16.
OM&P
Opera Med Physiol 2016 Vol. 2 (S1) 95
