CC BY
1Spectroscopic studies of cyanobacteria and its potential for
renewable energetics
E. Pei evedentseva'*, N. Melnik1, I. Elanskaya2, E. Muronets2, S. Savinov1, D. Korshunov3,
V. Ilyin3, E. Demikhov3
1- P.N. Lebedev Physical Institute of Rus. Acad. Sci. 119991 Moscow, Russia
2- Faculty of Biology, Moscow State University, 119991 Moscow, Russia 3- Institute of Biomedical Problems of Rus. Acad. Sci. 123007Moscow, Russia
* perevedencevaev@lebedev. ru
Identification of cyanobacteria and analysis of their distribution and role in ecological societies, study of the composition of various strains and metabolic processes in them are important tasks [1] for both scientific and applied research. Modern spectroscopic methods are a promising tool for solving relevant problems [2,3].
In the present research Raman spectroscopy and absorption (UV-visible) spectroscopy were used to investigate the cyanobacterium Synechocystis sp. PCC6803, as a model of oxygenic photosynthetic organisms, and its mutants with disruption of individual components of the photosynthetic or respiratory electron transport chains of (such as phycobiliproteins allophycocyanin and C-phycocyanin, flavodiironproteins, quinoloxidase, cytochrome c oxidase, etc.).
Combining UV-visible spectroscopy and Raman spectroscopy allows to analyse the structure of the cyanobacterial light-harvesting antennae [4] via observing phycobiliproteins (in particular, C-phycocyanin and allophycocyanin), structural components of the phycobilisomes, and considering carotenoids as component of compensatory mechanism of for overcoming the deficiency of phycobiliproteins acting like an additional internal antenna of photosystems, as an antioxidant (quencher of triplet chlorophyll and singlet oxygen) and as a photoprotector (protect the photosynthetic apparatus from excess excitation energy at high light intensity) [5]. Raman spectroscopy was applied for study of Photosystem II in dark-adapted cells and structural changes in the cell under following illumination with white light.
As cyanobacteria are recently considered quite promising for various biotechnological applications [1,6] the spectroscopic analysis can be used for monitoring of cyanobacteria in biotechnological production of substances or energy. In particular, using Raman for estimation of state of biomass and advantages of cyanobacteria use in the biomass for renewable energy generation are discussed.
[1] M.M. Allaf and H. Peerhossaini, Cyanobacteria: Model Microorganisms and Beyond, Microorganisms. 10(4), 696, (2022).
[2] I. Tanniche, E. Collakova, C. Denbow, R.S. Senger, Characterizing metabolic stress-induced phenotypes of Synechocystis PCC6803 with Raman spectroscopy, PeerJ, 8, e8535, (2020).
[3] I. Tanniche, E. Collakova, C. Denbow, R.S. Senger, Characterizing glucose, illumination, and nitrogen-deprivation phenotypes of Synechocystis PCC6803 with Raman spectroscopy, PeerJ, 8, e8585, (2020).
[4] E. Perevedentseva, N. Melnik, E. Muronets, A. Averyushkin, A. Karmenyan, I. Elanskaya, Raman spectroscopy with near IR excitation for study of structural components of cyanobacterial phycobilisomes, J Luminescence, 265, 120224, (2024).
[5] F. Pagels, V. Vasconcelos, A.C. Guedes, Carotenoids from Cyanobacteria: Biotechnological Potential and Optimization Strategies, Biomolecules 11(5), 735, (2021).
[6] K.B. Singh, Kaushalendra, S. Verma, R. Lalnunpuii, J.P. Rajan, Current Issues and Developments in Cyanobacteria-Derived Biofuel as a Potential Source of Energy for Sustainable Future, Sustainability 15(13), 10439, (2023).
