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Ukrainian Journal of Ecology
Ukrainian Journal of Ecology, 2020,10(3), 106-109, doi: 10.15421/2020_141
ORIGINAL ARTICLE
Species compositions of root rot agents of spring barley
D. T. Gentosh! M. M. Kyryk1, I. D. Gentosh1, M. Y. Pikovskyi1, V. M. Polozhenets1, S. V. Stankevych2, L. V. Nemerytska3, I. A. Zhuravska3, I.V. Zabrodina2, L. V. Zhukova2
1 National University of Life and Environmental Sciences of Ukraine, Kyiv, 03041, Ukraine 2V.V. Dokuchaev Kharkiv National Agrarian University, v. Dokuchaevske, Kharkiv region, 62483, Ukraine
3Zhytomyr Agricultural College, Zhytomyr, 10031, Ukraine
Corresponding author E-mail: [email protected]
Received: 13.06.2020. Accepted: 10.07.2020
We studied the ratio of fungi genera in the affected root system of spring barley, the composition of pathogenic and comorbid mycobiota, which affected barley at different ontogeny stages. We discovered that the main root rot agents were Fusarium spp., Bipolaris spp., Rhizoctonia spp., Pythium spp., Aiternaria spp., and the bacterium of Pseudomonas genera. In our research, 50.65% of Fusarium genera were taken at a sprouting stage from the affected root system, 54.25% were taken at tillering stage, and 56.25% were taken at milky-wax ripeness. The next abundant was B. sorokiniana fungus (20.6% of which were taken at sprouting stage, 18.4% at tillering stage, and 18.45% were taken at milky-wax ripeness. Less frequent were the representatives of Rhzzoctonia genera - 19.45, 16.15, and 13.2% respectively. We suggested that while studying the pathogenic characteristics of root rot agents of spring barley, more attention need to be paid to their impact on the laboratory germination ability of seeds, the degree of the disease progression, biometric indices and plants persistence.
Keywords: Spring barley; Root Rot; Species composition of the agents; Correlation of fungi genera; Pathogenic mycobiota; Phases of plants ontogeny
Introduction
Spring barley is the fourth plant in the world as to the area of its cultivation after wheat, rice and maize, and it is the second in Europe. In Ukraine, it comes second after wheat as to the area under cultivation and to crop page. It is stipulated by its food, grain and feed value, by its commercial value, high yield capacity, tolerance to environmental conditions and to agrotechnology. However, root rot prevents the increase in the crop yield. The species composition of the agents and the peculiarities of their development are of great importance for the efficient root rots control of spring barley. Root rots of grain crops - it is a disease of roots and radical part of the wheat, barley and rye stalks, which is caused by one type or complex of Fusarium, Helminthosporium, Ophiobulus, Pythium, and Rhzzoctonia genera (Korshunova et al., 1976; Nelson et al., 1983; Ashley et al., 2000).
The disease is manifested in roots affection, as well as in the affection of underground node, tillering node, stalk base and lower leaves. The affected roots and underground nodes get friable and break down when the plant is pulled from the soil. The tillering nodes get bulky and weak. The disease results in sprouting destruction, growth failure, and thin ears of the affected plants or in full decline of the productive stalks (Chulkina et al., 1974; Tepliakov & Tepliakova, 2003). Research aim was to study species composition of root rot agents of spring barley, to specify the correlation of fungi genera, which were taken from the affected root system of a crop, and to determine and specify the composition of pathogenic as well as of comorbid mycobiota, which affects the plants on different ontogeny stages of spring barley.
Materials and Methods
The dynamics of quantitative and qualitative composition of rhizosphere mycobiota of vegetative spring barley, was studied at the Field Research Station of National University of Life and Environmental Sciences of Ukraine during the vegetation periods of 20152017. Test samples were taken systematically regards the occurrence of disease symptoms by fungi etiology. The laboratory research was conducted at Plant Pathology Department in a basic research laboratory of "Mycology and Plant Pathology". The micromycetes - facultative parasites were harvested and identified, the morpho-cultural characteristics of mycocenosis components of spring barley were studied. Herewith, we used the method of direct inoculation on the breeding grounds (Bilai, 1982) and the method of fungi accumulation in moist chambers (Naumov, 1937; Pidoplichko, 1977, 1988). The occurrence rate of some fungi species was defined by T.G. Mirchink method (Mirchink, 1976).
On a provisional basis, species can be called typical when the frequency of their occurrence is higher than 30%, sporadic - when the frequency is lower than 10%. Those species whose occurrence was lower than 30% but higher than 10%, were related to as "undefined" species (Mirchink, 1976). The drawn out roots were repeatedly washed by sterile water, dried in a few layers of sterile filtrating paper, put into Petri dish and then into a germination chamber at a temperature of 26 °C. The fungi growth observation was conducted during 24-48 hours on the following days of their growing. The identification of micromycetes species was done according to Khohriakov (1974) and Bilai (1977).
Results and Discussion
We revealed that the main root rot agents were Fusarium spp. (51.66%), Bipolaris sorokiniana (21.16%), Rhizoctonia soiani (9.2%), Rhzzoctonia spp. (5.56%), Pythium spp. (4.1%), Aiternaria atternata (3.13%), Aiternaria spp. (3.2%), and other fungi taxa (2.96%) and the bacteria of Pseudomonas genera (Table 1).
Table 1. Ratio of fungi taxa, taken from an affected root system of spring barley (sort Sebastian, 2015-2017).
Ratio of taxa in the affected root system of spring barley, %
Fungi taxa
2015 2016 2017 average
Pythium spp. 4.3 4.9 3.1 4.1
Bipolaris sorokiniana 14.0 20.8 25.7 21.16
Rhizoctonia spp. 6.6 5.2 4.9 5.56
Rhizoctonia soiani 11.3 10.0 6.3 9.2
Fusarium spp. 53.2 50.2 51.6 51.66
Aiternaria spp. 4.7 2.1 2.8 3.2
Aiternaria alternata 2.8 3.0 3.6 3.13
Other genera 3.1 3.8 2.0 2.96
We concluded that Fusarium genera were the most frequent in the affected root system of spring barley within 2015-2017. In 2015, they amounted up to 53.2%, in 2016 - 50.2%, and in 2017 - up to 51.6% respectively. The ratio of Bipolaris sorokiniana to the total amount of isolates was the second after Fusarium spp. In 2015, they were reached 14.0%, in 2016 - 20.8%, and in 2017 - 25.7%. We accepted that the development of root rots in different crops is caused by many soil micromycetes, that is why it is necessary to determine and specify the composition of the pathogenic and comorbid mycobiota, which affects the plants at different ontogeny phases. In our research, main share of Fusarium genera (50.65%) were taken at a sprouting stage of the affected root system, 54.25% was taken at tillering stage, and 56.25% - at milky-wax ripeness - 56.25% (Figure 1a, Figure 2b and Table 1).
Table 2. Microbiological analysis of the affected roots of spring barley (sort Sebastian, 2015-2017).
Fungi genera Number of plants with the fungi, %
Sprouting Tillering Milky-wax ripeness
Pythium spp. 4.2 4.5 4.35
Bipolaris spp. 20.6 18.4 18.45
Fusarium spp. 50.65 54.25 56.25
Rhizoctonia spp. 19.45 16.15 13.2
Atternaria spp. 3.95 4.2 4.15
Other genera 1.15 2.5 3.6
Second frequent regards occurrence was Bipolaris fungus (20.6% were registered at sprouting stage, 18.4% - at tillering stage, and 18.45% - at milky-wax ripeness, Figure 2a). Much less frequent were the representatives of Rhizoctonia genera - 19.45, 16.15, and 13.2% respectively (Figure 2a and Figure 3). They were isolated together with the fusaria. A few representatives of different genera (Table 2) were often taken from the affected root system. The fungi of Pythium and Alternaría genera (Figure 1b and Figure 3) were identified in lower amounts - 4.2, 4.5, 4.35 and 3.95, 4.2, and 4.15%, respectively.
Figure 1. Fungus colony of Fusarium (a) and Aiternaria (b) genera isolated from the affected root system of spring barley (Czapek's medium, 7th day of cultivation).
Figure 2. Bipolaris sorokiniana (a) and Fusarium oxysporum (b), 40x.
Figure 3. Atternaria alternata, 40x.
While studying the pathogenic characteristics of root rots agents of spring barley, we also registered their impact on laboratory seeds sprouting and disease rate progression, biometrical indices and plants persistency. In laboratory conditions and under the influence of Fusarium species the seeds sprouting in F. oxysporum was decreased by 76.25%, in F. solani - by 73.75%, in F. graminearum- by 67.5%, and in F. avenaceum- by 65%; this was 80.0% in the control (Table 3).
Table 3. Pathogenicity of the spring barley isolates, affected by the root rot (sort Sebastian, laboratory experiment, 2016-2017).
Variant
Control without fungi introducing
Fusarium graminearum Fusarium avenaceum
Fusarium oxysporum Fusarium solani
Laboratory seeds sprouting, %
80.0 26.0 28.0 19.0 21.0
Sprouting Affected Disease
plants, %
12.0 61.0 59.0 75.0 71.0
progression
4.75 22.25 21.5 49.0 46.75
%
Plant heights cm %
18.7 10.3 10.7 8.2 8.6
100
55.1
57.2
43.8
45.9
The ratio of spring barley pathogenicity without the introducing of fungi was 12%, under the action of F oxysporum - 75.0%, F solani - 71.0%, F graminearum - 61.0%, and under the action of F avenaceum - 59.0%. The disease progression increased by 10.3, 9.8, 4.7, and 4.5 times respectively; the plant heights was less by 56.2, 54.1, 43.78, and 44.9%. These indices in the control were 4.75% and 18.7 cm (Table 3).
Conclusion
We established that rhizosphere mycobiota of the root system of spring barley is represented by 10 fungi genera. The most spread root rot agents of spring barley were Fusarium spp. (51.6%), Bipolaris sorokiniana (25.7%), Rhizoctonia solani (6.3%) Rhizoctonia spp. (4.9%), Pythium spp. (3.1%), Atternaria alternata (3.6%), and Alternaria spp. (2.8%). Other genera (2.0%) were represented by Mucor spp., Penicillium spp. and Aspergillus spp. The species composition of agents was not constant and depended on the meteorological conditions and plant development phase. We registered that the most pathogenic agents were F oxysporum and F solani. In laboratory conditions, the seeds sprouting decreased in F. oxysporum by 76.25%, in F solani - by 73.75% under the influence of these fungi; the disease progression increased by 10.3 and 9.8 times, the plants height was by
56.2% and 54.1% lower. The protection of spring barley against these agents would allow to decrease the intensity of root rots development and to increase the barley yield.
References
Ashley, R.O., Mullen, M.M., Eriksmoen, E., Schmidt, B., Barondeau, D., Duerre, D. & Eraas, K. (2000). Diagnosis and management of root disease in dryland wheat in southwest North Dakota. Dickinson Research Extension Center Annual Report. Available from: https://www.ag.ndsu.edu/archive/dickinso/research/2000/agron00p.htm Bilai, V.I. (1977). Fuzarii. Kiev, Naukova Dumka (in Russian).
Bilai, V.I. (1982). Metody eksperimentalnoj mikologii. Kiev. Naukova Dumka (in Russian).
Chulkina, V.A., Tabakayev, V.N. & Pahotnyuk, V.E. (1974). Osobennosti zarazheniya semyan yarovoj pshenicy i yachmenya fitopatogennymi gribami v razlichnyh ekologo-geograficheskih zonah Zapadnoj Sibiri. Borba s vreditelyami i boleznyami selskohozyajstvennyh kultur, Nauchno-tehnicheskij byulleten, 12, 3-11. (in Russian).
Khohriakov, M.K. (1974). Metodicheskie ukazaniya po eksperimentalnomu izucheniyu fitopatogennyh gribov. Leningrad. Nauka (in Russian).
Korshunova, A.F., Chumakov, A.F. & Shchecochihina, R.I. (1976). Zashita pshenicy ot kornevyh gnilej. Leningrad. Kolos (in Russian).
Mirchink, T.G. (1976). Pochvennaya mikologiya. Moscow. Moscow State University (in Russian).
Naumov, N.A. (1937). Metody mikologicheskih i fitopatologicheskih issledovani. Moscow. Selhozizdat (in Russian).
Nelson, P.E., Toussoun, T.A., Marasas, W.F.O. (1983). Fusarium species. University Park. The Pennsylvania State University Press.
Pidoplichko, N.M. (1977-1978). Griby - parazity kulturnyh rastenij. Vol. I-III. Kiev. Naukova dumka. (in Russian).
Tepliakov, V.I. & Tepliakova, O.I. (2003). Bolezni yarovoj pshenicy v Zapadnoj Sibiri. Zashita i karantin rastenij, 1, 17-18. (in Russian).
Citation:
Gentosh, D.T., Kyryk, M.M., Gentosh, I.D., Pikovskyi, MY., Polozhenets, V.M., Stankevych, S.V., Nemerytska, L.V., Zhuravska, I.A., Zabrodina, I.V., Zhukova, L.V. (2020). Species compositions of root rot agents of spring barley. Ukrainian Journal of Ecology, 10 (3), 106-109.
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