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0SERS substrates for glucose determination: a study on the use of polymer substrate functionalized with metal nanoparticles
O. Gusliakova'*, V. Bakal1, J. Cvjetinovic2, E. Prikhozhdenko1
1- Saratov State University, 83, Astrakhanskaya st., Saratov, 410012, Russian Federation 2- Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, Bolshoy
Boulevard 30, bld. 1, 121205 Moscow, Russia
* olga.gusliakova17@gmail.com
Optical approaches for glucose detection have received much attention in recent years due to their relatively low cost, portability, and low or noninvasive nature. Raman spectroscopy in combination with SERS substrates provides extremely high sensitivity due to signal amplification and specificity due to the unique vibrational coupling spectra of molecules of interest. However, direct detection of glucose using SERS substrates is difficult due to the poor adsorption of glucose onto clean metal surfaces and the low scattering cross section of glucose [1].
The results of screening a SERS platform based on polymer substrates for the quantitative determination of glucose in the concentration range specific to biological fluids are presented. In particular, configurations of glucose sensors based on PDMS and biocompatible polymer (PLGA, PCL) films modified with metal nanoparticles (gold nanorods and silver nanoparticles) were considered. PDMS substrates had an ordered array of depressions (of a given geometry). The possibility of using the wells themselves with a pre-modified surface with nanoparticles as microreservoirs has been considered, since in modern literature there is data on the amplification of the signal from glucose molecules upon repeated reflection of a laser beam, achieved by propagation in a cavity whose walls are covered with gold nanoparticles [2]. The technology of forming arrays of aggregates (of a given geometry) of metal nanoparticles by means of filling holes in a PDMS substrate and subsequent imprinting on a smooth surface, imitating the technique of obtaining microchambers [3], is also considered.
This research was funded by the Russian Science Foundation, grant number 22-79-10270, https://rscf.ru/en/project/22-79-10270/.
[1] X. Sun, Glucose detection through surface-enhanced Raman spectroscopy: A review, Analytica Chimica Acta, 1206, 339226, (2022).
[2] C.M. Jin, J.B. Joo, I. Choi, Facile amplification of solution-state surface-enhanced Raman scattering of small molecules using spontaneously formed 3D nanoplasmonic wells, Analytical chemistry, 90(8), 5023-5031, (2018).
[3] O.A. Sindeeva, O.I. Gusliakova, O.A. Inozemtseva, A.S. Abdurashitov, E.P. Brodovskaya, M. Gai, ... & G.B. Sukhorukov, Effect of a controlled release of epinephrine hydrochloride from PLGA microchamber array: in vivo studies, ACS applied materials & interfaces, 10(44), 37855-37864, (2018).
