CC BY
15
Turczaninowia 25, 3: 57-69 (2022) DOI: 10.14258/turczaninowia.25.3.6 http://turczaninowia.asu.ru
ISSN 1560-7259 (print edition)
TURCZANINOWIA
ISSN 1560-7267 (online edition)
УДК 582.382:581.96(513.21) Selaginella subeffusa, a new spikemoss from Southeast Yunnan, China
A. P. Shalimov1' 2 3, X.-C. Zhang2' 4*
1 Altai State University, Lenina Pr., 61, Barnaul, 656049, Russian Federation 2 Institute of Botany, Chinese Academy of Sciences (CAS), Beijing, 100093, China
3ORCID iD: https://orcid.org/0000-0002-8806-5217 4E-mail: zhangxc@ibcas.ac.cn; ORCID iD: https://orcid.org/0000-0003-3425-1011 Corresponding authors
Keywords: microspore, morphology, phylogeny, Selaginella effusa, Selaginella monospora, taxonomy, Yunnan.
Summary. Based on extensive herbarium research of the Selaginella monospora group (incl. S. effusa, S. microclada, S. monospora, S. subdiaphana, and S. submonospora) and on molecular phylogenetic evidence, an additional new species to this group was discovered. It was collected from Dawei Mountain in southeast Yunnan, and here named as Selaginella subeffusa. The new species distinctly differs from similar species by its coriaceous and densely imbricate ventral leaves on main stems and branches, 2.2-3.1 mm long and 1.1-1.5 mm wide, with enlarged acroscopic base, and strongly overlapping stem and branches, and with denticulate margins; microspores verrucate and perforate. Phylogenetic analyses of three plastid regions (rbcL, atpl, psbA) showed that S. subeffusa clustered with S. submonospora and nested between the "S. microclada - S. effusa" clade and the "S. monospora" clade.
Selaginella subeffusa - новый вид с юго-востока Юньнаня, Китай
А. П. Шалимов1, 2, С.-Ч. Чжан2
1 Алтайский государственный университет, пр. Ленина, д. 61, г. Барнаул, 656049, Россия 2 Институт ботаники Китайской Академии наук, г. Пекин, 100093, Китай
Ключевые слова: микроспоры, морфология, таксономия, филогения, Юньнань, Selaginella effusa, Selaginella monospora.
Аннотация. На основе обширных исследований гербарных образцов видов группы Selaginella monospora, включающей S. effusa, S. microclada, S. monospora, S. subdiaphana и S. submonospora, и молекулярно-филогене-тических данных был обнаружен новый вид в этой группе. Он был собран с горы Давей на юго-востоке провинции Юньнань и назван Selaginella subeffusa. Новый вид четко отличается от близких видов кожистыми вентральными листьями, плотно черепитчато расположенными по главному стеблю и боковым ветвям, 2,2-3,1 мм длиной и 1,1-1,5 мм шириной, зубчатыми по краю, с увеличенным акроскопическим основанием, сильно перекрывающими стебель и боковые ветви; микроспоры бородавчатые и перфорированные. Филогенетический анализ трех пластидных фрагментов (rbcL, atpl, psbA) показал, что образцы S. subeffusa сгруппированы с образцами S. submonospora и расположены между кладами "S. microclada - S. effusa" и "S. monospora".
Поступило в редакцию 12.05.2022 Принято к публикации 23.08.2022
Submitted 12.05.2022 Accepted 23.08.2022
Introduction
The genus Selaginella P. Beauv. is the largest lycophyte genus, comprising about 700-800 species world-wide (Jermy, 1990; Zhang, 2004; Zhang et al., 2013; Weststrand, Korall, 2016). It is distributed predominantly in tropical regions (Jermy, 1986), although it is adapted to live in diverse ecosystems (Jermy, 1990; Valdespino, 1993). Since the publication of "Flora of China" in 2013 (Zhang et al., 2013), several new species and new records of Selaginella have been reported (Wu et al., 2017; Zhang, 2018; Shalimov et al., 2019; Zhang et al., 2020; He et al., 2021; Yang et al., 2021; Huang et al., 2022; Shalimov, Zhang, 2022).
The Selaginella monospora Spring group includes: S. effusa Alston, S. microclada Baker, S. monospora Spring (syn. S. effusa var. dulongjiangensis W. M. Chu, S. medogensis Ching et S. K. Wu, S. monospora var. ciliolata W. M. Chu, S. trichophylla K. H. Shing), S. subdiaphana (Wall. ex Hook. et Grev.) Spring, S. submonospora Shalimov et X. C. Zhang, and the here described S. subeffusa Shalimov et X. C. Zhang. Species of this group are widely distributed from the Indo-Himalayan region to China, and south to Indochina and Indonesia (Shalimov, Zhang, 2021). This assembly is characterized by creeping or suberect, or rarely erect main stems (i. e. S. effusa), with vegetative leaves denticulate or denticulate to ciliolate at margin, and megaspores verrucate or papillate on proximal faces and vermiculate on distal faces with micro echinate ornamentation. The spore ornamentation pattern here described for the S. monospora group corresponds to what Zhou et al. (2015) called "the S. effusa subtype", which, however, is also found in S. labordei Hieron. ex Christ.
Material and methods
This study is mainly based on examination of herbarium specimens preserved at CSH, KUN, PE, PYU (acronyms follow Thiers, 2022) and online data sources: GBIF (https://www.gbif.org/) and CVH (https://www.cvh.ac.cn/en), which were used for additional verification of species determination. We examined high resolution type specimens of all concerned species treated in this study as described by Baker (1884), Alston (1932), Spring (1850), and Zhang et al. (2013).
Morphological observations were conducted using a Nikon DXM1200F camera connected to a stereomicroscope (Nikon SMZ 1000) and computer; measurements were done using D 3.10 software (http://www.nikoninstruments.com).
For scanning electron microscopy studies, dried and mature mega- and microspores were mounted on double-sided sticky tape, and sputter-coated with gold palladium. Spores were photographed and measured at different magnifications using a Hitachi S-4800 at 10-20 kV.
Phylogenetic analyses
For molecular analyses, total genomic DNA was isolated from silica dried material using the Plant Genomic DNA Kit (Tiangen Biotech, Beijing, China) following the manufacturer's protocol, primers, PCR condition of three chloroplast genes (rbcL, atpI, and psbA), and the phylogenetic analysis was conducted as described in Shalimov et al. (2019). All examined accession from GenBank and newly generated sequences of 73 samples used here are listed in Appendix 1.
Results and discussion Taxonomic treatment
Selaginella subeffusa Shalimov et X. C. Zhang, sp. nov. (Fig. 1, 2, 3).
Diagnosis. Selaginella subeffusa is morphologically intermediate between S. microclada and S. effusa, from which it is separated by its coriaceous leaves, ventral leaves densely imbricate on main stems and branches, each 2.2-3.1 x 1.1-1.5 mm; strobili 4.2-7.6 x 2.0-2.5 mm, and ventral and dorsal sporophylls margins denticulate.
Holotype: "China, Yunnan, Hekou, Dawei Mt., Sancha river, on forest slope, alt. 1120 m. 5 X 1999. Yu-Min Shui et al. 11716" (PE 01866084!).
Description. Plants terrestrial, evergreen, main stems creeping or suberect, robust, angulate, 15-30 cm long. Rhizophores restricted to base or proximal / of main stem and branches, borne on ventral side in axil of branches. Main stems pinnately branched several times, stramineous to reddish, 1-2 mm in diam. in lower part, stem oval or terete, some primary lateral branches developing into long branch systems; primary leafy branches 11-15 pairs, secondary branches once forked or 2-3 times pinnately branched, adjacent primary branches on main stem 1-3 cm apart; leafy portion of main stem 0.3-0.6 mm wide including leaves, ultimate branches 0.2-0.5 mm wide including leaves. Axillary leaves broadly-ovate or ovate-triangular, 1.4-2.5 x 0.9-1.4 mm, base obtuse, margin denticulate, apex acuminate. Dorsal leaves asymmetrical, leaves shifted to the side, ovate, 1.2-1.9 x 0.6-1.0 mm, carinate on abaxial surface, base obtuse, margin denticulate,
Fig. 1. Selaginella subeffusa Shalimov et X. C. Zhang, sp. nov. Digitized images of holotype: Yu-Min Shui et al. 11716 (PE 01866084).
Fig. 2. Selaginella subeffusa Shalimov et X. C. Zhang: A - Axillary leaf, lower surface; B - Dorsal leaves, upper surface; C - Ventral leaf, lower surface; D - Strobili, lower surface, d1 - close-up section of strobili. A-D from the holotype, Yu-Min Shui et al. 11716 (PE 01866084).
apex aristate. Ventral leaves asymmetrical, distant to imbricate, oblong or oblong-ovate, 2.2-3.1 x 1.1-1.5 mm, margin denticulate, apex subacute; basiscopic base decurrent, margin entire; acroscopic base rounded, strongly overlapping stem and branches, margin denticulate. Strobili solitary, terminal, compact, dorsiventrally complanate, 4.2-7.6 x 2.0-2.5 mm, strongly dimorphic, margins narrowly hyaline; dorsal sporophylls ovate-lanceolate, carinate with a complete and denticulate laminar-flap, margin denticulate, apex acuminate; ventral sporophylls ovate-triangular, strongly carinate
in central part, margin denticulate, apex long acuminate; megasporophylls in basal portion on ventral side of strobilus; microsporophylls in ventral and dorsal rows throughout strobili; microsporangia orbicular, rather thin with uniform cells; megaspores white, 253.8-330.7 |im in diam., verrucate but with few and distant verrucae on distal face, surface with vermiculate micro-sculpture of dense spinules; microspores yellowish orange, 25.2-32.4 |im in diam., densely verrucate on proximal and distal faces, micro-sculpture of surfaces perforate (Fig. 2).
Fig. 3. Selaginella subeffusa Shalimov et X. C. Zhang (Yu-Min Shui et al. 11716; holotype: PE 01866084): A, B, C -Megaspore, A - Proximal-equatorial face, B - Distal face, C - Close-up of megaspore surface, proximal face; D, E, F - Microspore, D - Proximal face, E - Distal face, F - Close-up of microspore surface, proximal face.
Ecology. Mountain slopes in evergreen forest; 1080-1120m.
Conservation status. Selaginella subeffusa is known from only two collections from Dawei Mountain, the exact size of each population is unknown. We tentatively assessed it as Data Deficient (DD) according to the IUCN (2021) categories and criteria.
Etymology. The specific epithet 'subeffusa' refers to its close relationship and morphological similarity to S. effusa.
Additional specimens examined (Paratype):
CHINA: "Yunnan, Pingbian, Dawei Mt. National Park, Alt. 1080 m. 8 II 2018. Yi-Fan Li and Lei Liu B180051" (CSH0150370, CSH0150371).
Fig. 4. The 50 % majority rule consensus tree derived from maximum likelihood showing the position of Selaginella subeffusa. Support values (BSML/PPBI) are shown above the main branches; the dash (-) indicates BS < 50 %. The new species name is boldfaced and highlighted in grey.
Phylogenetic Analysis
The concatenated matrix of three chloroplast DNA markers (rbcL+atpI+psbA) had 2042 characters, with 131 variable sites and 378 parsimony-informative sites. The reconstruction of the phylogeny tree derived from ML and BI analyses was topologically identical, hence the ML tree is shown in Fig. 4. Molecular data showed that S. subeffusa clustered with S. submonospora (Shalimov, Zhang, 2022), however, they are morphologically distinct. The S. subeffusa - S. submonospora clade is monophyletic (BS = 77, BP = 0.99) and sister to S. microclada - S. effusa clade (BS = 99, BP = 1) and S. monospora clade (BS = 99, BP = 1).
Morphological observations
Gross morphology
Selaginella subeffusa is morphologically similar to S. effusa but differs in its creeping or suberect main stems and branches (vs. erect in S. effusa), coriaceous and imbricate (vs. herbaceous and distant
or approximate) ventral leaves, and microspores Verrucae with perforate surface (vs. Verrucae or spherulate with smooth surface). It is also similar to S. microclada, especially in its habit and shape of lateral branches, densely arranged ventral leaves and amplexicaul dorsal leaves. However, S. subeffusa differs from the latter by its coriaceous (vs. herbaceous) leaves, strongly dimorphic (vs. slightly dimorphic) sporophylls. The most relevant morphological characters differentiating S. subeffusa from related species are summarized in Table and shown in Figure 5.
Spore morphology
Spore micromorphology of S. subeffusa was observed and documented (Fig. 3). Megaspores are verrucate with vermiculate micro-sculpture of dense spinules surface (Fig. 3, A, B, C). Morphologically similar megaspore features were reported for S. effusa, S. microclada, and S. monospora (Zhou et al., 2015; Wang et al., 2018; Shalimov, Zhang, 2021, 2022). However, microspores of S. subeffusa are
characterized by its irregularly sized Verrucae and closely related species as compared with images perforate micro sculpture surface (Fig. 3, D, E, F). provided in recent studies (i. e., Zhou et al., 2015; Furthermore, S. subeffusa is clearly distinct from Wang et al., 2018; Shalimov, Zhang, 2021, 2022).
Fig. 5. Comparison of Selaginella subeffusa leaves and strobilus with related species: 1A-D - Selaginella effusa (X.-C. Zhang et al. 5465, PE); 2A- D - S. subeffusa (Y.-M. Shui et al. 11716, PE); 3A-D - S. microclada (Feng 11473, PE); 4A-D - S. monospora (CPG Expedition CPG40561 (Y. J. Lai, Q. Zhang), PE). A - Axillary leaves, lower surface; B -Dorsal leaves, upper surface; C - Ventral leaves, lower surface; D - Strobili, lower surface. Scale bars = 0.2 mm (4B); 1 mm (1D, 2D, 4D); 0.5 mm (1A-1C, 2A-2C, 3A-3D, 4A, 4C).
Table
Comparison of Selaginella subeffusa and related species
Characters S. effusa S. subeffusa S. microclada S. monospora
Habit and size (cm) erect, 10-45 creeping, 15-30 long creeping, 35-85 or more creeping, 30-50
Axillary leaves shape ovate, ovate-triangular, or ovate-elliptic ovate, broadly-ovate or ovate-triangular ovate, narrowly ovate, or narrowly elliptic ovate
size (mm) 2-3.5 x 1.2-2.8 1.4-2.5 x 0.9-1.4 2-3 x 0.8-1.6 1.2-2.5 x 1-2
apex acuminate acuminate acuminate acuminate
margin ciliate denticulate denticulate denticulate-ciliate
Dorsal leaves shape obliquely ovate-elliptic ovate ovate-lanceolate or elliptic ovate
size (mm) 1.5-3.2 x 0.6-1 1.2-1.9 x 0.6-1.0 1-1.6 x 0.3-0.7 1.2-1.9 x 0.5-1
margin ciliate denticulate denticulate ciliate
apex aristate, arista curved, 0.8-1.6 mm aristate acuminate or shortly aristate aristate to cuspidate
Base of dorsal leaf subcordate or cuneate obtuse obtuse obtuse or subcordate
Ventral leaves shape oblong or oblong-ovate oblong or oblong-ovate ovate-triangular or oblong-falcate ovate-triangular, oblong or oblong ovate
size (mm) 2.2-5 x 1-2.2 2.2-3.1 x 1.1-1.5 2.6-5.5 x 0.9-2.3 2-3.2 x 0.8-1.6
margin denticulate denticulate denticulate denticulate, ciliate
apex subacute subacute subacute acute
Acroscopic margin of ventral leaves overlapping strongly overlapping stem and branches strongly overlapping stem and branches slightly overlapping stem and branches slightly overlapping stem and branches
margin ciliate along basal half, otherwise (denticulate apically) denticulate denticulate denticulate, ciliate
Basiscopic margin of ventral leaves ciliate at base, otherwise subentire apically entire throughout subentire or entire throughout ciliate at base, subentire from middle of laminate to apically
Strobili size (mm) 6.0-12.0 x 1.5-3.0 4.2-7.6 x 2.0-2.5 3.0-20.0 x 1.9-3.2 6.0-10.0 x 1.2-2.3
Dorsal sporophylls shape oblong-lanceolate ovate-lanceolate lanceolate ovate-lanceolate
margin ciliolate denticulate minutely denticulate denticulate
apex acute or acuminate acuminate acuminate acuminate
Ventral sporophylls shape ovate-triangular shortly ovate-triangular ovate-lanceolate ovate-lanceolate
margin ciliolate denticulate denticulate denticulate
Megaspore diameter (pm) 202.8-290.7* (160.0-280.0)233.3 x 258.7(173.3-320.0)** 253-330 305-420 280-432
ornamentation verrucae and/or papillate verrucae verrucae verrucae and/or papillate
surface subsmoth vermiculate vermiculate vermiculate
microsculptures spinulose* granulate/tuberculate densely spinulose spinulose densely spinulose
Table (continued)
Characters S. effusa S. subeffusa S. microclada S. monospora
diameter (pm) 26.54-33.19* 25-32 28-40 27-33
(17.0-28.8)23.7 x 29.8(20.9-39.2)**
Microspore ornamentation Verrucae or/and spherulate* tuberculate** Verrucae, surface perforate Verrucae, nearly rugulate Verrucae/ spherulate
micro- - - spinulose fine reticulate
sculptures
Note: More detail and photo see in: *Zhou et al. (2015), and ** Wang et al. (2018).
Key to Selaginella subeffusa and related species
1. Plants with long-creeping stems (up to 85 cm or more); sporophylls slightly dimorphic (sometimes appearing uniform); dorsal leaves ovate-lanceolate or elliptic; margin denticulate, apex acuminate or shortly aristate....................................... S. microclada
+ Plants with creeping, suberect, ascending or erect stems (less or up to 50 cm); sporophylls strongly dimorphic; dorsal leaves obliquely ovate-elliptic, broadly ovate-elliptic or ovate; margin denticulate or denticulate to ciliate, apex shortly acuminate or aristate to cuspidate ................................................... 2
2. Ventral leaves strongly overlapping on stem
and branches, acroscopic base enlarged, broader ....
....................................................................................... 3
+ Ventral leaves not overlapping on stem and
branches, acroscopic base not enlarged, oblique ......
............................................................ S. submonospora
3. Axillary and ventral leaves strongly overlapping stems and branches ................................................... 4
+ Axillary and ventral leaves slightly overlapping stems and branches ................................................... 5
4. Main stem erect, vegetative leaves herbaceous, ventral leaves distant or approximate ......... S. effusa
+ Main stem creeping or suberect, vegetative
leaves coriaceous, ventral leaves imbricate ...............
..................................................................... S. subeffusa
5. Ventral leaves ovate-triangular, at right angles to the stem; dorsal leaves with base subcordate, apex
aristate to cuspidate; megaspores white-yellow........
.................................................................. S. monospora
+ Ventral leaves ovate to ovate-lanceolate, at angles to the stem; dorsal leaves with base obtuse or slightly subcordate, apex short aristate; megaspores reddish-brown ................................... S. subdiaphana
Acknowledgements
We are grateful to curators and staffs of cited herbaria for providing access to their collections for this study. The first author is grateful for the support provided by the Chinese Academy of Sciences and the CAS-TWAS President's Fellowship for International PhD Students, which enable him to conduct this research while pursuing his training period under their sponsorship.
REFERENCES
Alston A. H. G. 1932. Notes on Selaginella - II. Journal of Botany, British and Foreign 70: 61-67.
Baker J. G. 1884. A synopsis of the genus Selaginella. Journal of Botany, British and Foreign 22: 241-247.
He M., He Z.-R. Zhang L.-B., Zhou X.-M. 2021. Selaginella qingchengshanensis (sect. Heterostachys; Selaginella-ceae), a new species from Sichuan, China. Phytotaxa 522(4): 285-293. DOI: 10.11646/phytotaxa.522.4.2
Huang L., Li S.-H., Dong S.-Y. 2022. Two new species of the Lycophyte genus Selaginella (Selaginellaceae) from China, with notes on the phylogenetic positions of related species. Systematic Botany 47(1): 85-96. DOI: 10.1600/036 364422X16442668423400
IUCN [2021]. The IUCN Red List of Threatened Species. Version 2021-3. URL: http://www.iucnredlist.org (Accessed 05 April 2022).
Jermy A. C. 1986. Subgeneric names in Selaginella. The Fern Gazette 13: 117-118.
Jermy A. C. 1990. Sellaginellaceae. In: K. Kubitzki (eds.) The families and genera of vascular plants. Vol. 1. Berlin: Springer. Pp. 39-45.
Shalimov A. P., ZhangX.-C. 2021. A taxonomic revision of Selaginella monospora Spring (Selaginellaceae). Turczaninowia 24, 3: 175-193. DOI: 10.14258/turczaninowia.24.3.14
Shalimov A. P., Zhang X.-C. 2022. Selaginella submonospora (Selaginellaceae), a new species from Yunnan. Turc-zaninowia 25, 1: 154-166. DOI: 10.14258/turczaninowia.25.1.15
Shalimov A. P., Zhu Y.-M., Zhang M.-H., Zhang X.-C. 2019. Selaginella dianzhongensis (Selaginellaceae), a new spikemoss from China. PhytoKeys 118: 75-87. DOI: 10.3897/phytokeys.118.30375
Spring A. F. 1850. Monographie de la Famille des Lycopodiacées, Seconde partie. Mémoires de l'Académie Royales des Sciences, Lettres et Beaux Arts de Belgique 24: 1-358.
ThiersB. [2022]. Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden's Virtual Herbarium. URL: http://sweetgum.nybg.org/science/ih/ (Accessed 5 April 2022).
Valdespino I. A. 1993. Selaginellaceae. In: Flora of North America North of Mexico. Vol. 2. Pteridophytes and gym-nosperms. Oxford: Oxford Univ. Press. Pp. 38-63.
Wang L., Zhang X.-C., Liu J. 2018. Studies on the complementary relationship of surface ornamentations between megaspores and microspores of Selaginella P. Beauv. (Selaginellaceae). Microscopy Research and Technique: 1-15. DOI: 10.1002/jemt.23148
Weststrand S., Korall P. 2016. A subgeneric classification of Selaginella (Selaginellaceae). American Journal of Botany 103(12): 2160-2169. DOI: 10.3732/ajb.1600288
Wu Y.-D., Zhang H.-R., Zhang X.-C. 2017. Selaginella guihaia (Selaginellaceae): A new spikemoss species from southern China and northern Vietnam around the Gulf of Tonkin. PhytoKeys 80: 41-52. DOI: 10.3897/phytok-eys.80.11126
Yang J., Zhang M.-H., Wang Y.-R., Yuan L.-X., Zhang X.-C. 2021. An extraordinary rosette and resurrection new spikemoss, Selaginella iridescens (Selaginellaceae) from Hainan Island, China. Taxonomy 1: 302-312. DOI: 10.3390/ taxonomy1040024
Zhang M.-H., Wei R., Xiang Q.-P., Ebihara A., Zhang X.-C. 2021. Integrative taxonomy of the Selaginella helvetica group based on morphological, molecular and ecological data. Taxon 70(6): 1-25. DOI: 10.1002/tax.12565
Zhang X.-C. 2004. Selaginellaceae. In: X.-C. Zhang (ed.). Flora Republica Popularis Sinicae. Vol. 6. Beijing: Science Press. Pp. 86-219.
Zhang X.-C. 2018. Some new records of Selaginella from China. Philippine Journal of Systematic Biology 12, 1: 22-23.
Zhang X.-C., Nooteboom H. P., Kato M. 2013. Selaginellaceae. In: Z. Y. Wu, P. H. Raven, D. Y. Hong (eds.). Flora of China. Vols 2-3. Beijing: Science Press, St. Louis: Missouri Botanical Garden Press. Pp. 37-66.
Zhang X.-C., Shalimov A. P., Kang J.-S., Zhang M.-H. 2020. Selaginella subvaginata (Selaginellaceae), a new spikemoss from China. Journal of Species Research 9(3): 221-232. DOI: 10.12651/JSR.2020.9.3.221
Zhou X.-M., JiangL.-J., ZhangL., Gao X.-F., HeZ.-R., ZhangL.-B. 2015. Spore morphology of Selaginella (Selaginellaceae) from China and its systematic significance. Phytotaxa 237(1): 1-67. DOI: 10.11646/phytotaxa.237.1
Appendix
Specimen information and GenBank accession numbers (* indicates new accession)
Taxon Voucher Country rbcL atpI psbA
Selaginella albociliata P. S. Wang Zhang X.-C. 7242 (PE) Guizhou, China MH814882 MH814826 MH814854
Selaginella amblyphylla Alston Zhang X.-C. 2924 (PE) Yunnan, China MH814883 MH814827 MH814855
Selaginella amblyphylla Alston Zhang X.-C. 7951 (PE) Yunnan, China MH814884 MH814828 MH814856
Selaginella bodinieri Hieron. Zhang X.-C. 5 (PE) Chongqing, China MH814885 MH814829 MH814857
Selaginella bodinieri Hieron. Zhang X.-C. 526 (PE) Sichuan, China MH814886 MH814830 MH814858
Selaginella bodinieri Hieron. Zhang X.-C. 7069 (PE) Guizhou, China MH814887 MH814831 MH814859
Selaginella brachystachya (Hook. et Grev.) Spring Sledge W. A. 913 (L) Sri Lanka KY022979 - -
Selaginella brachystachya (Hook. et Grev.) Spring Klackenberg J. 434 (S) Sri Lanka KY022980 - -
Selaginella f. reticulata Fraser-Jenkins C. R. 1653 (L) Nepal KY022956 - -
Appendix (continued)
Taxon Voucher Country rbcL atpI psbA
Selaginella chaetoloma Alston Guo Z.-Y. 2016014 (PE) Guizhou, China MH814888 MH814832 MH814860
Selaginella chaetoloma Alston Zhang X.-C. 7347 (PE) Guizhou, China MH814889 MH814833 MH814861
Selaginella chingii Alston Zhang X.-C. 7904 (PE) Guangxi, China MH814890 MH814834 MH814862
Selaginella chrysocaulos (Hook. et Grev.) Spring Gaoligong Shan Biodiversity Survey 27010 (GH) China, Yunnan KY022989
Selaginella chrysocaulos (Hook. et Grev.) Spring Li H. 12202 (GH) China, Yunnan KY022990 - -
Selaginella ciliaris (Retz.) Spring Zhang X.-C. 7780 (PE) Yunnan, China MH814892 MH814836 MH814864
Selaginella coriaceifolia X. M. Zhou, N. T. Lu et Li Bing Zhang Zhang L.-B. et al. 7307 (CDBI, MO, VNMN) Quang Binh, Vietnam MT386596
Selaginella coriaceifolia X. M. Zhou, N. T. Lu et Li Bing Zhang Zhang L.-B. et al. 7347(CDBI, MO) Quang Binh, Vietnam MT386597
Selaginella coriaceifolia X. M. Zhou, N. T. Lu et Li Bing Zhang Zhang L.-B. et al. 7371 (CDBI, MO, VNMN) Quang Binh, Vietnam MT386598
Selaginella decipiens Warb. Zhang X.-C. 1130 (PE) Guangxi, China MH814893 MH814837 MH814865
Selaginella deflexa Brack. Palmer 2651(KANU) Hawaii AF093253
Selaginella delicatula Alston Gao & al. HGX10734 (CDBI) Sichuan, China KT161441
Selaginella dianzhongensis X.- C. Zhang Zhu Y.-M. 8158 (PE) Yunnan, China MH814909 MH814853 MH814881
Selaginella effusa Alston Zhang 5438 (CDBI) Guangxi, China KT161450 - -
Selaginella effusa Alston Zhang 5442 (CDBI) Guangxi, China KT161451 - -
Selaginella effusa Alston Wang 20051 (CDBI) Guizhou, China KT161452 - -
Selaginella effusa var. dulongjiangensis W. M. Chu Chu et al. 31299 (PYU) Yunnan, China KT161449 - -
Selaginella heterostachys Baker Zhang X.-C. 7088 (PE) Guizhou, China MH814896 MH814840 MH814868
Selaginella heterostachys Baker Zhang X.-C. 7268 (PE) Guizhou, China MH814897 MH814841 MH814869
Selaginella hezhangensis P. S. Wang et X. Y. Wang FLPH Sichuan Expedition 152002 Sichuan, China OM864654 OM864639 OM864647
Selaginella hezhangensis P. S. Wang et X. Y. Wang Jiang X.-L. 35003 (PE) Sichuan, China OM864655 OM864640 -
Selaginella hezhangensis P. S. Wang et X. Y. Wang Zhang X.-C. 6830 (PE) Guizhou, China OM864656 OM864641 OM864648
Selaginella hezhangensis P. S. Wang et X. Y. Wang Wei R., Yang J. WRO547 Sichuan, China OM864657 OM864642 OM864649
Appendix (continued)
Taxon Voucher Country rbcL atpI psbA
Selaginella kraussiana (Kunze) A. Braun Zhou 062 (CDBI) Yunnan, China, Cult. KT161498 - -
Selaginella kurzii Baker Zhang X.-C. 1934 (PE) Yunnan, China MH814898 MH814842 MH814870
Selaginella labordei Hieron. ex Christ Zhang X.-C. 3356 (PE) Hubei, China MH814899 MH814843 MH814871
Selaginella laxistrobila K. H. Shing Chu et al. 24449 (PYU) Yunnan, China KT161509 - -
Selaginella leptophylla Baker Zhang 5853 (CDBI) Guizhou, China KT161510 - -
Selaginella leptophylla Baker Zhang 5199 (CDBI) Guangxi, China KT161511 - -
Selaginella leptophylla Baker Zhou 011 (CDBI) Sichuan, China KT161512 - -
Selaginella leptophylla Baker Zhou et al. DJY05380 (CDBI) Sichuan, China KT161513 - -
Selaginella medogensis Ching et S. K. Wu Qinghai-Xizang Complex Exped. 74-4375 (PE) Tibet, China OK247696
Selaginella megaphylla Baker Jin X.-H. 19301 (PE) Tibet, China MH814901 MH814845 MH814873
Selaginella miniatospora (Dalzell) Baker C. van Hardeveld, H. van der Werff 120 (U) Tamil Nadu, India KY023080
Selaginella miniatospora (Dalzell) Baker Klackenberg J., Lundin R. 567 (S) Kerala, India KY023081 - -
Selaginella microclada Baker Zhang X.-C. 7889 (PE) Guangxi, China MH814902 MH814846 MH814874
Selaginella microclada Baker Zhang X.-C. et al. 8107 (PE) Yunnan, China OK247701 OK247684 OK247716
Selaginella monospora Spring Yan Y.-H. et al. WYD427 (PE) Guangdong, China OK247712 OK247695 OK247725
Selaginella monospora Spring CPG Expedition CPG40561 (PE) Meghalaya, India OK247709 OK247692 OK247722.1
Selaginella monospora Spring Zhang X.-C. et al. 8121 (PE) Yunnan, China OK247705 OK247688 OK247720
Selaginella monospora Spring Zhang X.-C. et al. 8122 (PE) Yunnan, China OK247706 OK247689 OK247721
Selaginella monospora Spring Zhang X.-C. 2992 (PE) Yunnan, China OK247703 OK247686 OK247718
Selaginella monospora Spring Yan Y.-H. et al. WYD404 (PE) Guangdong, China OK247711 OK24769 OK247724
Selaginella monospora Spring Zhang X.-C. 6016 (PE) Guangxi, China OK247704 OK247687 OK247719
Selaginella monospora Spring Wang Y.-R. 9370 (PE) Hainan, China OK247707 OK247690 -
Selaginella monospora Spring Wang Y.-R. 9371 (PE) Hainan, China OK247708 OK247691 -
Selaginella monospora var. ciliolata W. M. Chu Lu S. G., Zhang G. F. 27625-B (PE) Yunnan, China OK247710 OK247693 OK247723
Selaginella nipponica Franch. et Sav. Zhou et al. DJY07479 (CDBI) Sichuan, China KT161542 - -
Appendix (continued)
Taxon Voucher Country rbcL atpI psbA
Selaginella ornata (Hook. et Grev.) Spring Zhang X.-C. 8520 (PE) Yunnan, China MH814903 MH814847 MH814875
Selaginella remotifolia Spring Zhou 005 (PYU, CDBI) Yunnan, China KT161580 - -
Selaginella repanda (Desv. ex Poir.) Spring Zhang X.-C. 5655 (PE) Yunnan, China MH814904 MH814848 MH814876
Selaginella repanda (Desv. ex Poir.) Spring Zhang X.- C. 9273 (PE) Yunnan, China MH814905 MH814849 MH814877
Selaginella repanda (Desv. ex Poir.) Spring Li B.-G. sn 20 (PE) Yunnan, China MH814906 MH814850 MH814878
Selaginella selaginoides (L.) P. Beauv. ex Schrank et Mart. Weststrand S. 104 (UPS) Sweden KY023148 - -
Selaginella subdiaphana (Wall. ex Hook. et Grev.) Spring Zhang X.-C. 5297 (PE) Tibet, China OM864658 0M864643 0M864650
Selaginella subeffusa Shalimov et X. C. Zhang Shui Y.-M. et al. 11716 (PE) Yunnan, China ON711493* 0N711491* 0N711492*
Selaginella submonospora Shalimov et X. C. Zhang Zhang X.-C. et al. 8111 (PE) Yunnan, China 0M864660 0M864646 0M864653
Selaginella trichophylla K. H. Shing Zhang et al. 6784 (CDBI, MO, VNMN, PYU) Cao Bang, Vietnam KT161624
Selaginella trichophylla K. H. Shing Chu et al. 31925 (PYU) Yunnan, China KT161621 - -
Selaginella trichophylla K. H. Shing Chu et al. 29310 (PYU) Yunnan, China KT161622 - -
Selaginella trichophylla K. H. Shing Jiang 318 (PYU, CDBI) Hainan, China KT161623 - -
Selaginella vaginata Spring Zhang Z.-S. 161 (PE) Shaanxi, China MH814907 MH814851 MH814879
Selaginella xipholepis Baker Zhang X.-C. 7422 (PE) Guizhou, China MH814908 MH814852 MH814880
