Научная статья на тему 'Seeking markers to distinguish HCV-infected-autoimmune subjects from uninfected-autoimmune patients'

Seeking markers to distinguish HCV-infected-autoimmune subjects from uninfected-autoimmune patients Текст научной статьи по специальности «Фундаментальная медицина»

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Russian Open Medical Journal
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AUTOIMMUNITY / B-CELL ACTIVATION FACTOR / HEPATITIS C VIRUS / IL-10 / TNF-α

Аннотация научной статьи по фундаментальной медицине, автор научной работы — Maghraby Amany S., Kamel Solaf A., Fayed Dalia B., Shawky Heba, Razin Mona A.

Introduction Egypt has the highest prevalence of hepatitis C virus (HCV) infection which is reported to be associated with autoimmune manifestations. Aim There is a need for markers that enable differentiation between viral and non-infected-autoimmune patients. Material and Methods Levels of the B-cell activation factor (BAFF), IL6, IL10 and TNF-α were quantified by ELISA in sera from HCV-infected-autoimmune patients (HCV-AI), HCV-infected patients with no autoimmune diseases (HCV-NAI), non-infected autoimmune patients (NIAI) and healthy control humans (C). Results HCV-infected patients were 12.96, 8.33, 69.44 and 36.11% positive for the auto-antibodies ANA, AMA, ASMA and AGPCA respectively. The BAFF level was significantly (P<0.05) higher among the NI-AI patients compared to the HCV-NAI, HCV-AI patients and healthy control humans (C). For the IL6, no significant differences were seen between various patients' groups. In case of the IL10, its, levels were significantly higher (P<0.05) in the HCV-AI, NI-AI and HCV-NAI patients in comparison to the C humans. Levels of TNF-α were significantly (P<0.001 and 0.0001) higher amongst the HCV-NAI and HCV-AI than in the NI-AI patients respectively. Also, levels of the TNF-α were significantly (P<0.0001) higher amongst the C compared to the NI-AI. Levels of TNF-α were not significant amongst the HCV-NAI and HCV-AI patients than in C subjects. Conclusion The obtained results showed that both IL6 and BAFF can serve as markers for autoimmunity and/or autoimmunity resulting from severe HCV infection, whereas, IL10 and TNF-α can be considered as markers for HCV infection in Egypt (genotype is mostly 4a).

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Текст научной работы на тему «Seeking markers to distinguish HCV-infected-autoimmune subjects from uninfected-autoimmune patients»

2019. Volume 8. Issue 3. Article CID e0308 DOI: 10.15275/rusomj.2019.0308_

Infectious Diseases

Original article

Seeking markers to distinguish HCV-infected-autoimmune subjects from uninfected-autoimmune

patients

Amany S. Maghraby, Solaf A. Kamel, Dalia B. Fayed, Heba Shawky, Mona A. Razin, Rola N. Abd-Elshafy, Azza F.

Arafa, Dina N. Abd-Elshafy, Mahmoud M. Bahgat

The National Research Centre, Cairo, Egypt

Received 10 October 2018, Revised 1 May 2019, Accepted 8 August 2019

© 2018, Maghraby A.S., Kamel S.A., Fayed D.B., Shawky H., Razin M.A., Abd-Elshafy R.N., Arafa F.A., Abd-Elshafy D.N., Bahgat M.M. © 2018, Russian Open Medical Journal

Abstract: Introduction — Egypt has the highest prevalence of hepatitis C virus (HCV) infection which is reported to be associated with autoimmune manifestations.

Aim — There is a need for markers that enable differentiation between viral and non-infected-autoimmune patients. Material and Methods — Levels of the B-cell activation factor (BAFF), IL6, IL10 and TNF-a were quantified by ELISA in sera from HCV-infected-autoimmune patients (HCV-AI), HCV-infected patients with no autoimmune diseases (HCV-NAI), non-infected autoimmune patients (NIAI) and healthy control humans (C).

Results — HCV-infected patients were 12.96, 8.33, 69.44 and 36.11% positive for the auto-antibodies ANA, AMA, ASMA and AGPCA respectively. The BAFF level was significantly (P<0.05) higher among the NI-AI patients compared to the HCV-NAI, HCV-AI patients and healthy control humans (C). For the IL6, no significant differences were seen between various patients' groups. In case of the IL10, ^ levels were significantly higher (P<0.05) in the HCV-AI, NI-AI and HCV-NAI patients in comparison to the C humans. Levels of TNF-a were significantly (P<0.001 and 0.0001) higher amongst the HCV-NAI and HCV-AI than in the NI-AI patients respectively. Also, levels of the TNF-a were significantly (P<0.0001) higher amongst the C compared to the NI-AI. Levels of TNF-a were not significant amongst the HCV-NAI and HCV-AI patients than in C subjects.

Conclusion — The obtained results showed that both IL6 and BAFF can serve as markers for autoimmunity and/or autoimmunity resulting from severe HCV infection, whereas, IL10 and TNF-a can be considered as markers for HCV infection in Egypt (genotype is mostly 4a).

Keywords: Autoimmunity, B-cell activation factor, hepatitis C virus, IL10, TNF-a

Cite as Maghraby AS, Kamel SA, Fayed DB, Shawky H, Razin MA, Abd-Elshafy RN, Azza FA, Abd-Elshafy DN, Bahgat MM. Seeking markers to distinguish HCV-Infected-autoimmune subjects from uninfected-autoimmune patients. Russian Open Medical Journal 2019; 8: e0308.

Correspondence to Amany Sayed Maghraby. Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research and Research Group Immune- and Bio-markers for Infection, the Centre of Excellence for Advanced Sciences, the National Research Centre, Cairo, Egypt. Fax: 002033371718. E-mail: [email protected]. Phone: 00201226489703.

Introduction

Egypt has the highest prevalence of HCV infection (14.7%) worldwide [1] where the prevailing genotype (90%) is mostly 4a [2, 3]. An Egyptian study compared HCV prevalence in 2015 with a national survey made in 2008; results demonstrated ~29% reduction in HCV RNA prevalence and added that approximately 3.7 million persons are chronically infected with HCV in the age group between 15-59 years [4]. The drop in HCV prevalence between the two reports could be attributed to the national control efforts or due to significant reduction in the prevalence of human schistosomiasis, a parasitic disease whose infection is thought to be highly related to HCV infections [4, 5].

It was reported that a total of 40-74% of HCV infected patients may experience immunological complications during the course of the disease [6, 7]. Autoimmunity is a break in self-tolerance involving stimulation of auto-antibodies producing self-reactive B cells and

formation of complement-fixing immune complexes ending by tissue damage [8].

Infection by HCV was reported to be associated with various autoimmune manifestations [9]. In addition to the liver, HCV may exist in other tissues such as kidney, skin and salivary glands [10]. Such tissues may represent reservoirs for the virus and contribute to both persistence and viral reactivation [10]. It was postulated that replication and expression of HCV antigens in chronically HCV infected patients in these tissues may play a role in development of autoimmune manifestations [10]. Some autoimmune diseases were reported in Egypt like rheumatoid arthritis with prevalence of 21%, [11] autoimmune hepatitis with prevalence of 32% [5] and diabetes with prevalence of 12.6% in Egypt under age of 15 [12].

Rheumatic diseases, arthralgia, arthritis, vasculitis, sicca syndrome, myalgia, and fibromyalgia are examples for inflammatory and autoimmune reactions associating HCV infection [13]. The

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Infectious Diseases

mechanism by which HCV triggers autoimmune reactions and production of auto-antibodies was attributed to expansion of CD5+ subpopulation and over-expression of CD81 in the peripheral B-cells from chronically infected patients in response to increase of HCV viral load [14]. The same authors demonstrated positive correlation between auto-antibodies with both cirrhosis and age and postulated that titer and type of such auto-antibodies might help in diagnosis and treatment of infection.

Cytokines were frequently reported as markers for both HCV infection [15, 16] and autoimmune diseases [17, 18]. Contradictory results were published on levels of IL6 among HCV infected humans. While some demonstrated its decrease upon infection, [19] others showed its increase [15, 16, 20]. Interestingly, a study concluded IL6 as valuable markers for severity of HCV infection as its serum levels were higher among patients presenting severe infection compared to milder ones and also patients with genotype 1 showed higher serum levels of IL-6 than those with genotype 2 [21].

A study on Egyptian patients' demonstrated higher serum IL10 and TNF-a levels compared to non-infected control [15, 16, 22]. B cell activating factor (BAFF) is essential in B lymphocytes development, maturation and activation [23]. Cytokines, such as IFNs and IL-10 activate myeloid cells including macrophages and dendritic cells to produce BAFF [23]. Increased BAFF stabilizes auto-reactive B cells leading to autoimmune pathogenesis [23]. Supportively, Egyptian juvenile idiopathic arthritis patients had significantly higher levels of BAFF that correlated with both clinical manifestations and disease activity [24]. The interrelationship between BAFF and HCV infection emerges from the capacity of virus to induce multiple B cell disorders while developing chronicity [25]. In concordance, an increasing association was found between BAFF-871C/T promoter polymorphism and HCV-related mixed cryoglobulinemia [26].

Apart from HCV infection, cytokines were also reported as useful markers in several autoimmune diseases. Secretion of IL-6 and TNF-a was promoted among juvenile idiopathic arthritis patients [17]. IL-6 production was shown to be significantly higher among neuromyelitis optica patients compared to healthy humans [27].

Altogether highlights importance of monitoring autoimmmune pathogensis among HCV infected humans which will enable early discovery and hence treatment of the onset of the autoimmune manifestation. Alternatively, it will be of great relevance to study the susceptibility of autoimmune patients to HCV infection. Supportively, it was reported that an autoimmune disease known as membranoproliferative glomerulonephritis (MPGN) was treatable upon anti-HCV therapy [28].

Therefore, a primary aim of the work was to investigate the influence of autoimmune pathogenesis on HCV-load among Egyptian patients. A second major objective was to investigate any potential differential regulation of several immune markers namely BAFF, IL6, IL10 and TNF-a amongst HCV-infected-autoimmune Egyptian patients, HCV-infected Egyptian patients presenting no autoimmune diseases and non-infected autoimmune patients hoping to identify a marker which can distinguish between the three groups. Last aim upon identifying such marker(s) was to introduce a recommendation of frequent screening of Egyptian patients presenting autoimmune disease for active HCV infection and vice versa.

Material and Methods

Subjects and experimental design

The study involved 68 Egyptian subjects who were divided into 4 groups. Group (1): 10 healthy subjects. Fifty HCV infected patient's candidates for interferon therapy (direct-acting antiviral for the treatment of HCV-infection-DAAS; were not available in Egypt at the time of collected samples from 2012 to 2014) were divided into group 2 and 3 according to the positivity of the immunodiagnostic tests to detect autoimmune antibodies. Twenty-seven HCV infected patients were negative for the diagnostic test of auto-immune antibodies (HCV-NAI) and considered as group (2). Twenty-three HCV infected patients were positive for the diagnostic test of auto-immune antibodies (HCV-AI) and considered as group (3). Eight patients were positive for autoimmune antibodies and negative for HCV diagnosis (AI) and considered as group (4): Whole blood samples were collected from all subjects. Serum was separated, divided into aliquots and stored at -800C until being used.

Detection of viral load by Real-Time PCR (qrtPCR)

HCV-RNA was quantitatively detected in sera by the Abbott real time HCV Kit (Abbott Molecular Inc; Des Plaines, IL 60018 USA) which uses RT-PCR conjugated with real time fluorescent. The assay is standardized against the second WHO international standard for HCV-RNA (NIBSC Code 96\798) and results are reported in IU\mL [29].

Detection of liver enzymes and a1-fetoprotein (AFP)

Liver complications due to viral infection were biochemically assessed by quantifying liver enzymes and AFP. The quantitative determinations of both the aspartate aminotransferase/glutamate oxaloacetate trasaminase, (ASAT\GOT) and alanine aminotranferase\glutamate pyruvate transaminase, (ALAT\GPT) were carried out according to the manufacture instructions (Roche automated clinical chemistry analyzers, cobas®, City, Country). AFP assay was performed according to the manufacturer instructions (Roche; cobas®, Hitachi, Japan).

Detection of auto-antibodies by indirect immunofluorescence assay

The indirect immunofluorescent assay was performed on slides mounted with mouse tissues (kidney, liver and stomach) to test presence of auto-antibodies in sera namely anti-nuclear antibodies (ANA), anti-smooth muscle antibodies (ASMA) and anti-mitochondrial antibodies (AMA). Serum samples were diluted in phosphate buffer saline (PBS). A FITC-labeled polyclonal rabbit anti-human-IgG was used for fluorescence visualization of the autoimmune reactions. On each tested slide, a negative control (IFA System Negative Control; INOVA Diagnostics, San Diego CA USA) was included.

Quantification of cytokines and BAFF in patients' sera by enzyme-linked immunosorbent assay (ELISA)

Levels of IL-6, IL-10 and TNF-a in patients' sera were detected by ELISA using reagent kits from (KOMA BIOTECH INC.; Seoul, Korea). The changes in the optical densities (OD) were recorded at X-max 450 nm using a multi-well plate reader (Tecan; Sunrise, Austria, GmbH). The concentrations of IL-6, IL-10 and TNF-a were calculated according to equations of the experimental standard curves.

2019. Volume 8. Issue 3. Article CID e0308 DOI: 10.15275/rusomj.2019.0308_

Infectious Diseases

Figure 1. Detection of autoimmune antibodies using indirect immune fluorescent assay. ANA (anti-nuclear antibodies), ASMA (anti-smooth muscle antibodies), AMA (anti-mitochondrial antibodies) (X40).

Figure 2. HCV load (A), levels of aFP (B) and liver enzymes (C&D) among HCV-infected-autoimmune (HCV-AI) patients and HCV-infected-non autoimmune (HCV-NAI) patients. Figure was in box-plot form.

Levels of BAFF/BLyS in human sera were determined using the quantikine human BAFF/BLyS/TNF134B ELISA kit Minneapolis, MN USA). The detected levels were compared to standard recombinant human BAFF/BLyS to enable accurate quantification. The changes in the OD were recorded at X-max 450 nm using a multi-well plate reader.

Statistical analysis

Statistical analysis and plots were done using the GraphPad PRISM version 5 software. Figures were in box-plot form. The degree of significance was calculated using the unpaired data comparison application (F- test) to compare variances. Differences were considered significant when P-values were <0.05, 0.001, 0.0001; while "ns" means none significant.

Results

Among the HCV-infected patients the positivity rates of the ANA, AMA ASMA and AGPCA were 12.96, 8.33, 69.44 and 36.11% respectively (Figure 1).

The quantified HCV copy number was significantly lower amongst the autoimmune patients (AI) compared to the non autoimmune individuals (NAI; P<0.05; Figure 2A). Although no significant differences were seen in the levels of a-FP, GOT and GPT between the two patients' groups the three parameters were respectively 0.87, 0.88 and 0.90 fold higher among the NAI compared to the AI (Figure 2B).

Figure 3. Comparison of B-cell activation factor (BAFF as A1, A2 and A3) and cytokines' profiles (IL-6 as B1, B2 and B3, IL-10 as C1, C2 and C3 and TNF-a as D1, D2 and D3) in HCV-infected-autoimmune (HCV-AI) patients, HCV-infected-non-autoimmune patients (HCV-NAI) patients,HCV-infected-autoimmune (HCV-AI) patients, non-infected-autoimmune patients (NI-AI) patients, and healthy control humans (C).

2019. Volume 8. Issue 3. Article CID e0308 DOI: 10.15275/rusomj.2019.0308_

Infectious Diseases

The B-cell activation factor (BAFF) level was significantly (P<0.05) higher among the non-infected autoimmune patients (NIAI) compared to the HCV-infected non-autoimmune patients (HCV-NAI; Figure 3: A1), the HCV-infected autoimmune patients (HCV-AI; Figure 3: A2) and the healthy control humans (C; Figure 3: A1 and A2). Although the HCV-AI showed 1.25 fold higher level than the HCV-NAI the difference was not significant. Also, non-significant higher levels were seen in either of the HCV-AI or HCV-NAI compared to the C and the fold differences were 1.45 and 1.16, respectively (Figure 3A3).

For the IL6, no significant differences (P>0.05) were seen between various patients' groups. However, the NI-AI showed 0.82 and 0.97 fold higher levels than the HCV-NAI and the HCV-AI, respectively, whereas, the levels were 1.19 fold higher among the HCV-AI compared to the HCV-NAI (Figure 3: B1, B2). Although no significant difference was seen in the cytokine level between all patients' groups and the healthy controls the levels were 0.64, 0.79 and 0.76 fold higher among the C compared to HCV-NAI, NI-AI and HCV-AI groups, respectively (Figure 3: B1, B2 and B3).

In case of the IL10, although HCV-AI showed 1.53 fold higher levels than the HCV-NAI the difference was not significant (Figure 3: C3); however, its levels were significantly higher (P<0.05) in the HCV-AI, NI-AI and HCV-NAI in comparison to the C (Figure 3: C1, C2 and C3).

Levels of TNF-a were significantly (P<0.001 and 0.0001) higher amongst the HCV-NAI and HCV-AI than in the NI-AI patients respectively (Figure 3: D1, D2). Also, levels of the TNF-a were significantly (P<0.0001) higher amongst the C compared to the NIAI (Figure 3: D2). Levels of TNF-a were not significant amongst the HCV-NAI and HCV-AI patients than in C subjects (Figure 3: D3).

Discussion

Both HCV persistence [30, 31] and IFN-a therapy [32] trigger autoimmune reactions resulting in production of auto-antibodies such as ANA, [33] AMA, ASMA and AGPCA [34, 7]. In the present study the most reactive autoantibody among Egyptian HCV-infected patients was the ASMA with a recorded prevalence of 69.44% and approximately similar results were obtained by Clifford et al [35] while, others reported that its reactivity ranged between 12-66% based on technical reasons and\or differences in the populations investigated [36].

Our results demonstrated that approximately 13% of HCV treated Egyptian patients with interferon (DAAS were not available in Egypt at the time of collected samples from 2012 to 2014) were positive for ANA and this agrees with Wilson et al [37] who reported that 4% to 19% of patients receiving interferon develop auto-immune disorders and Cassani et al [38] who found that 9% of HCV infected patients were positive for ANA. ANA was reported to be a useful candidate biomarker for diagnosing of HCV as it was positive in a big proportion of infected patients [7] and its prevalence in chronic HCV infected humans increased after treatment and this was a possible effect triggered by IFN-a therapy [39].

The previously reported frequency of AMA in autoimmune hepatitis patients varied significantly across different reports and ranged between 5-20% [40] and our recorder prevalence (8.33%) of such autoantibody among Egyptian HCV-infected subjects fell within this range.

De Olivera Andrade et al [41] reviewed that the measuring of anti-GPCA level in chronic HCV patients and treated with INF is very important although, the frequency of anti-GPCA are less encountered in HCV infected patients [42]. Our study demonstrated that among Egyptian HCV-infected patients the positive rate of AGPCA autoimmune antibodies was 36.11%. From the results of us and others the frequent association between the four known auto-antibodies with HCV infection/therapy is obvious, yet, one cannot absolutely conclude that the trigger of their production is mainly due to infection and or INF-therapy or due to any other known extrahepatic autoimmune manifestation such as arthralgia, myalgia, rheumatoid arthritis, sicca, lupus erythematosus or vasculitis [43], which complicates identifying the exact source of the detected autoantibody in case of HCV infection.

In our hands the quantified HCV copy number was significantly lower amongst the AI patients compared to the NAI patients and our possible simple explanation of the decreasing HCV load in AI patients could be due to the antiviral capacity of the triggered autoimmune reactions within AI patients' group as autoimmunity is known to involve not only stimulation of auto-antibodies production but also trigger both self-reactive B cells and formation of complement-fixing immune complexes [8]. Along the same lines, HCV in chronically infected patients triggers production of auto-antibodies through expansion of CD5+cells and overexpression of CD81 on peripheral B-cells which positively correlate with both cirrhosis and age and can function as helpful markers for diagnosis and treatment of infection [16]. Although the positive correlation between the titers of auto-antibodies with cirrhosis seems to contradict the arrest of the virus propagation by the auto-antibodies, one can postulate that it can reflect both blockage of infection and severe liver inflammation due to the induced autoimmune reactions leading to development of autoimmune hepatitis and ending with cirrhosis.

Our results showed that a-FP levels were higher among the HCV-NAI compared to the HCV-AI patients. Wojtowicz-Chomicz et al [44] found that a-FP levels frequently increased in patients with chronic HCV even in the absence of HCC and declined during antiviral therapy. Also, a-FP concentration was reported to be higher in patients suffering from autoimmune hepatitis Yamagiwa et al [45] or Sjogren's syndrome [46].

Our results showed that levels of GOT and GPT were 0.88 and 0.9 fold higher among the HCV-NAI compared to the HCV-AI patients, respectively. Significantly higher GOT and GPT levels were observed by others in autoimmune hepatitis patients [47] and in a case of polymyositis associated with chronic active hepatitis [48]. In addition, significantly higher GOT levels were found in type 1 diabetics as an example of autoimmune disease [49]. One possible reason why the recorded liver enzymes levels were not significantly higher among the AI in our case is anti-HCV treatment.

The cytokine profile plays an important role in treatment outcome of HCV infection, and probably modulates the immune response against HCV [50]. Chronic HCV and stages of cirrhosis were associated with misbalance in production of antiinflammatory cytokine [50].

The higher levels of IL6 among the NI-AI compared to the HCV-NAI and the HCV-AI, knowing that all the study subjects in the present report were receiving interferon therapy, these results agree with Freeman et al [51] who reported that interferon Alfa

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Infectious Diseases

and Lambda treatment increased IL-6 levels in serum of HCV-infected patients. Our results also showed that IL-6 levels were 1.19 fold higher among the HCV-AI compared to the HCV-NAI. Assuming that AI might correlates with severity of infection, this agrees with previously published report [2] which interestingly described IL-6 as valuable markers for severity of HCV infection as its serum levels were higher among patients presenting severe infection compared to milder ones.

The recorded higher serum BAFF levels in the present study among the NI-AI compared to the HCV-NAI, the HCV-AI and the healthy control humans or among the HCV-AI compared to the HCV-NAI subjects suggest BAFF to be more likely a marker for autoimmunity and not for infection. Tobui et al [52] reported increased serum BAFF levels in patients with chronic HCV, and in patients with HCV-related autoimmunity. On the other hand, Migita et al [53] demonstrated that Serum BAFF levels were elevated in autoimmune hepatitis patients compared with healthy subjects.

The results of the present work showed that both HCV-AI and HCV-NAI patients had significantly higher levels of IL-10 compared to healthy controls which agrees with both Piazzolla et al [50] who showed that serum IL-10 levels were higher among chronic HCV patients compared to controls, and El-Emshaty et al [22] reported the same on Egyptian subjects. Mikadze and Vashakidze [54] reported a significant rise of IL-10 concentration in cases of severe hepatic lesions. Similarly, in our hands levels of TNF-a were higher amongst the HCV-NAI and HCV-AI than in the NI-AI patients and this agreed with reported higher levels of the same cytokine among HCV-infected patients compared to healthy controls [15, 16].

Conclusion

The obtained results allow the conclusion that both IL6 and BAFF can serve as markers for autoimmunity and/or autoimmunity resulting from severe HCV infection, whereas, IL10 and TNF-a can be considered as markers for HCV infection.

Limitations

The present study was performed on HCV infected patient's candidates for interferon therapy. Direct acting antiviral for the treatment of HCV-infection (DAAS) were not available in Egypt at the time (2012 to 2014) of collected samples.

Funding

This study was supported by the awarded grant No. 10010203 from the National Research Center (NRC) of Egypt.

Acknowledgements

The authors acknowledge the National Research Centre (NRC), Egypt for providing all needed facilities and logistics for the work. This study was supported by the grant from NRC (Project Reference number: 10010203).

Conflict of Interest

The authors have no conflict of interests related to this publication.

Ethical approval

All procedures performed in the study were in accordance with the ethical standards of the institution or practice at which the study was conducted. Human blood samples collection were approved by the Medical

Ethical Committee of the National Research Center in Egypt according to

the National Institutes of Health in the USA (Project Reference number:

10010203 from the National Research Center of Egypt).

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Authors:

Amany S. Maghraby - PhD, Professor, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research and Research Group Immune- and Bio-markers for Infection, the Centre of Excellence for Advanced Sciences, the National Research Centre, Cairo, Egypt. https://orcid.org/0000-0002-9723-5011.

Solaf A. Kamel - PhD, Professor, Department of Clinical and Chemical Pathology, the National Research Center, Cairo, Egypt. https://orcid.org/0000-0002-9342-3672.

Dalia B. Fayed - PhD, Researcher, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research, the National Research Centre, Cairo, Egypt. https://orcid.org/0000-0001-5238-7516. Heba Shawky - PhD, Researcher, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research, the National Research Centre, Cairo, Egypt. https://orcid.org/0000-0001-8403-1143. Mona A. Razin - MSc, Researcher Assistant, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research, the Centre of Excellence for Advanced Sciences, the National Research Centre, Cairo, Egypt. https://orcid.org/0000-0003-1900-6632. Rola N. Abd-Elshafy - MSc, Researcher Assistant, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research and Research Group Immune- and Bio-markers for Infection, the Centre of Excellence for Advanced Sciences, the National Research Centre, Cairo, Egypt. https://orcid.org/0000-0002-2777-2487. Azza F Arafa - PhD, Assistant Professor, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research, the National Research Centre, Cairo, Egypt. https://orcid.org/0000-0001-7729-24874.

Dina N. Abd-Elshafy - PhD, Assistant Professor, Department of Water Pollution Research, Environmental Research Division and Research Group Immune- and Bio-markers for Infection, the Centre of Excellence for Advanced Sciences, the National Research Centre, Cairo, Egypt. https://orcid.org/0000-0002-2777-2487.

Mahmoud M. Bahgat - PhD, Professor, Department of Therapeutic Chemistry, Division of Pharmaceutical and Drug Industries Research and Research Group Immune- and Bio-markers for Infection, the Centre of

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