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Abstracts. PHYTOPHARM 2017
SCREENING OF MEDICINAL PLANTS FOR THEIR ANTILEPTOSPIRAL ACTIVITY AND SYNERGISTIC RESPONSE WITH AMPICILLIN
© Chantana Mekseepralard1, Weeranuch Seesom2, Sudarat Sondonprai1,
Nirawan Kitprapiumpon3, Faongchat Jarintanan4, Wanlaya Tanechpongtamb2
1 Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok,Thailand;
2 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand;
3 College of Oriental Medicine, Rangsit University, Pathum Thani, Thailand;
4 Facuty of Medical Technology, Rangsit University, Pathum Thani, Thailand
Leptospirosis is a zoonotic disease of global distribution, which is caused by spirochete bacteria of the genus Leptospira. The disease in humans has been usually treated with a full course of antibiotics. Crude plant extracts have been traditionally used as herbal medicine which is an alternative way for the treatment of human infectious diseases for long time. This study investigated crude plant extracts for antileptospiral potential and examined the synergistic effect with ampicillin against both nonpathogenic and pathogenic leptospires. The crude extracts of Curcuma zedoaria (rhizomes), Gymnopetalum cochinchinense (fruits), Terminalia chebula (galls), Piper sarmentosum (leaves), and Tinospora crispa (stems) were tested for their minimal inhibitory concentrations (MIC) against a nonpathogenic Leptospira biflexa serovar Patoc and five pathogenic L. interrogans serovars Autumnalis, Hebdomadis, Pyrogenes, Ranarum, and Sejroe by using broth
microdilution assay. The synergistic effect of ampicillin and the crude extract was also evaluated by calculating the fractional inhibitory concentration (FIC) index. Among five medicinal plants, the extract of Terminalia chebula showed the highest antileptospiral property with the MIC varying from 0.0098-0.625 mg/ml whereas the rest of the extracts gave the MIC at 0.078 to > 2.5 mg/ml. Interestingly, synergism and partial synergistic response at FIC index of 0.318 and 0.57 were observed when the extract of T. chebula was added to ampicillin against L. interrogans serovar Hebdomadis and Sejroe, respectively. However, antagonistic effect against L. interrogans serovar Autumnalis was also generated. This study revealed that all the medicinal plants tested were able to inhibit the growth of leptospires with various MIC values. Synergy between the potent crude extract and ampicillin may enhance the drug efficacy for the treatment of leptospirosis.
ISOLATION AND DETERMINATION OF ANTIOXIDANT CAPACITIES OF ANTHOCYANIN FROM ROSA CANINA FRUITS
© Mahmoodreza Moein12, Zahra Sabahi1, Fatemeh Farmani1
1 Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;
2 Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Anthocyanins, particular group of flavonoid, are one of the known pigment in plants and their product [1]. The literature has been determined several potential health benefits of these natural compounds such as anticarcinogenic activities, protective effect against cardiovascular disease and anti-inflammatory effect [2]. Several phytochemichal compounds such as flavonoids, tannins, carotenoids, fatty acids, phospholipids, minerals, vitamins were determined in fruits of Rosa canina [3].
In this study, ethanol extract of freeze dried fruit of Rosa canina was partitioned between ethyl acetate and 0.3% TFA. The aquatic fraction was loaded on column containing amberlite XAD-7 as a stationary phase eluted
with 0.3% TFA in water and 0.3% TFA in methanol, respectively. Fraction eluted with 0.3% TFA in methanol was collected as a rich source of anthocyanin and analyzed by HPLC-UV. The antioxidant activities of this fraction are showed in Table 1. Also, lipid peroxidation inhibition of that demonstrated in fig. 1.
The results demonstrated that the high level of antioxidant capacity of anthocyanin of Rosa canina fruits by different methods to scavenge free radicals such as DPPH, nitric oxide and ABTS. It seems that this anthocyanin is potent lipid peroxidative inhibitor. Further findings in this area may be provided the therapeutic strategies in radicals associated diseases and in vivo studies.
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1
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