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1Физика Водных Растворов
WATER AND ITS DIELECTRIC SIGNATURE NEW MARKER FOR BIOSENSING
Feldman YD.
The Hebrew University of Jerusalem, Institute of Applied Physics, Edmond J. Safra Campus, Jerusalem 91904, Israel e-mail: [email protected]
Whenever water molecules interact with either dipolar or charged systems, the main water dielectric relaxation peak broadens. If a solute is dipolar in nature, new solute-water clusters are created due to dipole-dipole interactions. It leads to the "red shift" of the dielectric loss maximum frequency. In the case of ionic solutions, another cluster structure develops, due to dipole-charge interactions and a "blue shift "is observed. In the general case when a solute molecule has both charged and dipole groups, the dielectric loss maximum demonstrates a "red" or "blue" shift, depending on the entity concentration. In all aqueous solutions, the water-solute interactions can be considered as dipole-matrix interactions in which water is the dipole subsystem. The phenomenological 3D trajectories approach was applied to the results of isothermal dielectric measurements of different concentrations of the following aqueous solutions: Hydrocarbons, NaCl and KCl, AMP and ATP, Amino Acids and proteins [1-5]. The parameters of the main water peak define a trajectory that can clarify the nature and rate, at which water interacts with the solute. In this paper, we extend this approach from comparatively simple solutions to the complexity of Red Blood Cells (RBC) suspensions by monitoring the RBC cytoplasm under different external conditions [6,7]. Dielectric measurements of RBC suspensions in the frequency region of 100 MHz to 50 GHz as a function of aging or external glucose concentration also reveal a distinct time point or glucose concentration after which the spectra are radically changed. The conclusion is that the dielectric response of the cytoplasm in microwaves is due to the water therein and its interaction with physiological active components in cytoplasm. This opens a window of opportunity to exploit this for the non-invasive monitoring of diabetes or to non-invasive control of the quality of Stored RBC in a Blood bank in order to manage the inventory.
References:
1. E. Levy, A. Puzenko, U. Kaatze, P. Ben Ishai, Y. Feldman, Dielectric spectra broadening as the signature of dipole-matrix interaction. I. Water in nonionic solutions, J. Chem. Phys., (2012) 136, 114502.
2 E. Levy, A. Puzenko, U. Kaatze, P. Ben Ishai, Y. Feldman, Dielectric spectra broadening as the signature of dipole-matrix interaction. II. Water in ionic solutions J. Chem. Phys., (2012) 136, 114503.
3. A. Puzenko, E. Levy, A. Shendrik, M.S. Talary, A. Caduff, Y. Feldman, Dielectric spectra broadening as a signature for dipole-matrix interaction. III. Water in adenosine monophosphate/adenosine-5'-triphosphate solutions, The Journal of chemical physics, (2012) 137,194502.
4. E. Levy, S. Cerveny, I. Ermolina, A. Puzenko, Y. Feldman, Dielectric spectra broadening as a signature for dipole-matrix interaction. IV. Water in amino acids solutions, J. Chem. Phys., (2014) 140 135104.
5. L. Latypova, A. Puzenko, E. Levy, Yuri Feldman, "Dielectric spectra broadening as a signature for dipole-matrix interactions. V. Water in protein solutions" J. Chem. Phys. (2020) Vol. 153, 045102-8 https://doi.org/10.1063/5.0016437
6. E. Levy, G. Barshtein, L. Livshitz, P. Ben Ishai1 and Yu. Feldman, "The Vitality of Human RBC and its connection to cytoplasmic water: I. The glucose concentration influence" Journal Physical Chemistry B (2016), 120, 10214.
7. E. Levy, M. David, G. Barshtein, S. Yedgar, L. Livshits, P. Ben Ishai, and Yu. Feldman, "Dielectric Response of Cytoplasmic Water and Its Connection to the Vitality of Human Red Blood Cells: II. The influence of storage", Journal Physical Chemistry B (2017), 121, 5273.
