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экспериментальная и клиническая гастроэнтерология | выпуск 117 | № 5 2015
информация | information
ABSTRACTS IN ENGLISH (A-Z)
110. Roles of neuronal or endothelial synthases of nitric oxide while control of gastric bicarbonate secretion induced by mild irritation of the mucosa
Andreeva J. V., Kropycheva R. P., Zolotarev V. A.
Pavlov Institute of Physiology of the Russian Academy of Sciences, St. Petersburg, Russian Federation, e-mail: basil@infran.ru
An increase of synthesis of endogenous nitric oxide (NO) in the gastric mucosa (GM) depending on activity of constitutive synthases of NO at the normal physiological condition is considered as the important factor of gastric protection. However, the role of endogenous NO in regulation of gastric bicarbonate secretion, the main process in gastric protection, is still poorly understood. In particular, effects of endothelial (eNOS) and neuronal (nNOS) synthases of nitric oxide, which are present almost in all types of GM cells, were not compared.
The aim of the study: to estimate involvement of nNOS or eNOS in control of bicarbonate production induced by transient irritation of GM at the normal condition under anesthesia, as well as after blocking of gastric acid secretion by omeprasol.
In anesthetized rats, concentration of HCO3- in stomach luminal perfusate was calculated basing on measurements of pH/PCO2. Mucosal irritation during 20 min with acidic hypertonic solution (1 M NaCl, pH=2,0)
caused marked and omeprazole-independent increase of HCO3- secretion. Selective blocker of nNOS in vivo, 7-nitroindazole (7-NI), or the nonselective blocker of nNOS and eNOS, NG-nitro-L-arginine (L-NNA), were applied into the splanchnic cyrculation via retrograde injection into the splenic artery (1 mg/kg). At the nor-mo-acid state, irritation-induced output of HCO3- was suppressed by 7-NI, but was not affected by L-NNA. After administration of omeprazole, both 7-NI and L-NNA equally inhibited HCO3-- output. Effect of 7-NI (but not L-NNA) was abolished by cyclooxygenase (COX), an inhibitor indomethacin, which by itself suppressed secretion of HCO3-. Additionally, bicarbonate output was substantially reduced by the blocker of soluble guanylate cyclase (GC), methylene blue. We conclude that irritation-induced secretion of HCO3- is largely mediated by intramural nNOS and depends on GC-COX interaction. As it was theoretically estimated, eNOS activity caused a reduction of HCO3- output in the normo-acid stomach which was abolished by omeprazole.
111. Non-farmacological treatment of NAFLD/NASH in Bardejov Spa
Belovicovâ M.U3, Svirkovâ H.1, Bachul'ak V.1
'Internal-hepatologic ambulance, 2Remedium s.r.o., 3VSZaSP sv. Alzbety, Bardejovské Kupele, Slovakia, e-mail: mriab9@gmail.com, mbelovicova@kupele-bj.sk
Background. Non-alcoholic fatty liver disease (NA-FLD) represents a wide range of clinical and pathological conditions which are gradually developed from simple hepatic steatosis through steatohepatitis (NASH) up to the stage of liver cirrhosis with its complications and failure. The epidemic of obesity and the associated NAFLD is the most common disease which we meet in our ambulances.
The aim of the study: to evaluate the non-farmaco-logical treatment of NAFLD in Bardejov Spa.
Material and methods. Bardejov Spa developed in 2012 a specialized 2-week spa stay focused on weight reduction. During this stay clients acquire new knowledge regarding diet, physical activity, are under medical supervision. Clients undergo testing to early detection of cardiovascular diseases and liver diseases. They have the opportunity to take the examination with transient elastography and abdominal ultrasonography.
Results. This spa-stay completed 151 clients (116 women, 35 men). The mean BMI in women was 36.5 kg/m2, in men 38.1 kg/m 2. The age of participants: in women 20-73 years, in male 20-70 years. Average for 2 weeks, we have achieved a weight loss of 2.6 kg in women, in men of 5.4 kg. Waist circumference in women on average decreased by 4.7 cm, in men by 5.4 cm. Thanks methodology transient elastography at 18 clients (11.9 % of the group) we diagnosed the presence of liver cirrhosis on the basis of NASH.
Conclusions. Treatment of obesity/NAFLD in spa versus outpatient treatment has its advantages. First there is at least 2-week presence of patienta at the spa — outside ofthe standard environment and the overall orientation of stay for lifestyle change. During the stay patients are always in a good psychological well-being. Spa treatment of obesity and NAFLD at the same time allows the group intervention and mutual motivation of patients.
112. Treatment of chronic constipation in stroke patients with diabetes
Bukreeva M. V., Koroleva L. U., Turchina M. S.
Orel State University, Russia, e-mail: Bukreyka@rambler.ru.
Objective: to evaluate the effectiveness of various schemes of treatment of chronic constipation in stroke patients with diabetes.
Materials and methods. In the study group included 67 stroke patients with type 2 diabetes. Patients by simple randomization were divided into 3 groups: the first group
received a dose of lactulose 30 ml/day in combination with a dose trimebutin 0.6 mg/day — 4 weeks. The 2nd group received trimebutin in a dose of 0.6 mg/day — 4 weeks. The third group received lactulose dose of 50 ml/ day 4 weeks. To assess the quality of life of patients by using SF-36 questionnaire at baseline and after therapy.
