CC BY
265
Journal of Stress Physiology & Biochemistry, Vol. 9 No. 3 2013, pp. 318-325 ISSN 1997-0838 Original Text Copyright © 2013 by Restrepo-Diaz, Garces-Varon
ORIGINAL ARTICLE
Response of rice plants to heat stress during initiation of panicle primordia or grain-filling phases
Hermann Restrepo-Diaz1, Gabriel Garces-Varon2
1 Facultad de Agronomia Universidad Nacional de Colombia, Sede Bogota, Edifico 500, Oficina 229, Colombia
2 Federacion Nacional de Arroceros, Centro Experimental las Lagunas, Saldana, Colombia
*E-Mail: hrestrepodCa unal.edu.co
Received April 10, 2013
Leaf photosynthesis, a major determinant for yield sustainability in rice, is greatly conditioned by high temperature stress during growth. The effect of short-term high temperatures on leaf photosynthesis, stomatal conductance, Fv/Fm, SPAD readings and yield characteristics was studied in two Colombian rice cultivars. Two genotypes, cv. Fedearroz 50 (F50) and cv. Fedearroz 733 (F733) were used in pot experiments with heat stress treatment (Plants were exposed to 40°C for two and half hours for five consecutive days) and natural temperature (control) treatment. Heat treatments were carried out at the initiation of panicle primordial (IP) or grain-filling (GF) phases. The results showed that short-term high temperature stress produced a reduction on the photosynthesis rate in both cultivars either IP or GF phases. Similar trends were found on stomatal conductance in all cases due to high temperatures. Although Fv/Fm and SPAD readings were not affected by high temperatures, these variables diminished significantly among phenological phases. 'F733' rice plants showed higher number spikelet sterility due to heat stress treatments. These results seem to indicate that heat-tolerant cultivars of rice is associated with high levels of photosynthesis rate in leaves.
Key words: Chlorophyll fluorescence, Indica rice, stomatal conductance, photosynthesis, heat stress
ORIGINAL ARTICLE
Response of rice plants to heat stress during initiation of panicle primordia or grain-filling phases
Hermann Restrepo-Diaz1, Gabriel Garces-Varon2
1 Facultad de Agronomia Universidad Nacional de Colombia, Sede Bogota, Edifico 500, Oficina 229, Colombia
2 Federacion Nacional de Arroceros, Centro Experimental las Lagunas, Saldana, Colombia
*E-Mail: hrestrepodCa unal.edu.co
Received April 10, 2013
Leaf photosynthesis, a major determinant for yield sustainability in rice, is greatly conditioned by high temperature stress during growth. The effect of short-term high temperatures on leaf photosynthesis, stomatal conductance, Fv/Fm, SPAD readings and yield characteristics was studied in two Colombian rice cultivars. Two genotypes, cv. Fedearroz 50 (F50) and cv. Fedearroz 733 (F733) were used in pot experiments with heat stress treatment (Plants were exposed to 40°C for two and half hours for five consecutive days) and natural temperature (control) treatment. Heat treatments were carried out at the initiation of panicle primordial (IP) or grain-filling (GF) phases. The results showed that short-term high temperature stress produced a reduction on the photosynthesis rate in both cultivars either IP or GF phases. Similar trends were found on stomatal conductance in all cases due to high temperatures. Although Fv/Fm and SPAD readings were not affected by high temperatures, these variables diminished significantly among phenological phases. 'F733' rice plants showed higher number spikelet sterility due to heat stress treatments. These results seem to indicate that heat-tolerant cultivars of rice is associated with high levels of photosynthesis rate in leaves.
Key words: Chlorophyll fluorescence, Indica rice, stomatal conductance, photosynthesis, heat stress
Rice is one of the most important food crops in Colombia, where occupies a total area of 420.721 ha, with a production of 2.262.055 tons in 2010 (Fedearroz, 2011). Currently, global climate change has caused detrimental effects on yield physiology of rice because of the rise in atmospheric temperatures (Fageria, 2007; Jagadish et al., 2007). Likewise, environmental situations such as El Nino phenomenon or prolonged period of time without
rains (dry season) can also cause severe conditions of heat stress in rice-growing areas at certain periods of the year in Colombia (Castilla et al., 2010). Photosynthesis is one of the most susceptible processes to heat stress among the physiological processes in plants (Yin et al., 2010). Photosynthesis of rice leaves are considerably reduced at temperatures higher than 35°C (Taniyama et al., 1988). Heat stress can also affect
mechanisms such as rubisco activation state, maximal efficiency of PSII photochemistry (Fv/Fm), the actual PSII efficiency in the light-adapted state and/or non-photochemical quenching in rice leaves (Yin et al., 2010). On the other hand, some adverse environmental factors such as drought, low solar radiation, N deficiency, low or high temperatures in the initiation of panicle primordial and/or spikelet filling stage can increase spikelet sterility and consequently grain yield (Fageria, 2007).
In Colombia, rice is presently grown in regions where temperatures are far to the optimal for growth (28/22°C); hence, it is common to find episodes of high temperature during sensitive stages could reduce yields (Castilla et al., 2010). Several reports concerning with the physiological responses of rice leaves to heat stress have been published among cultivars of both indica and japonica types (Cao et al., 2009; Taniyama et al., 1988; Yang and Heilman, 1993; Yin et al., 2010). However, the most of these studies are mainly performed in temperate or subtropical regions. The information about photosynthesis studies of rice cultivars in tropical regions, especially, Latin America is scarce. In addition, little is known about the physiological behavior of Colombian cultivars; a better knowledge on the susceptibility and/or tolerance to heat stress would contribute to improve breeding programs. For these reasons, the aim of this work was to study the effects of shortterm high temperature stress during initiation of panicle primordia or grain-filling stages on physiological mechanisms such as chlorophyll fluorescence, stomatal conductance and photosynthesis.
MATERIALS AND METHODS
This research was conducted between June and
October of 2010 at "Centro de Investigacion Las Lagunas" Experimental farm in Saldana, Colombia. Two indica rice cultivars (cv. Fedearroz 50 (F50) and cv. Fedearroz 733 (F733)) were used. Both cultivars are widely cultivated by Colombian rice growers. Plants were placed outdoors and cultivated into 2 L pots containing soil as substrate. The soil was sandy loam that contained organic matter at 2%. Each plastic pot contained two rice plants. Each pot was provided with 1g N, 0.3g P2O5, and 0.7g K2O as a top dressing about 45 days before heading. The plants were irrigated in excess twice a day, early morning and late afternoon, to keep the soil saturated. The average day-time temperature (06h00-18h00) was ~29.5°C. The average night-time temperature (18h00-06h00) was~25.6°C. Relative humidity was 68.1% in the day time and 85.3% at night. The maximal irradiance at noon was about 1516 W m-2. The experiment lasted 120 days.
Both cultivars were split into three treatments: a) plants without heat stress (Control), b) plants under heat stress at the initiation of panicle primordia (IP), and c) plants under heat stress at grain-filling (GF). Sun-lit phytotron (KBW-400, Binder, Germany) was used for short-term high temperature stress. Plants were exposed to 40°C for two and half hours (09h30-12h00) for five consecutive days to achieve short-term high temperature stress. Then, plants were returned back to outdoors.
Measurements of net photosynthetic gas exchange (Pn) and stomatal conductance (gs) were performed on a fully expanded attached leaf of rice seedlings by a portable photosynthesis system (LI-6200; LICOR, Inc., Lincoln, NE) using a 250-cm3 cuvette, and a leaf-porometer (SC - 1 Decagon Devices, Inc, Pullman, WA), respectively. Two sets of 3-days measurements [27-29 July and 10-12
September (referred to as "IP" and "GF", respectively)] were carried out between 09h00 and 13h00. During all gas exchange measurements, conditions within the cuvette were: PAR greater than 800 ^mol m-2 s-1, leaf temperature 27 ± 5°C, and leaf to air water vapor pressure difference 1.8 ± 0.5 kPa.
Leaf chlorophyll fluorescence measurements were carried out at 31 July (IP), and 13 September (GF) using a continuous excitation chlorophyll fluorescence analyzer (Handy PEA; Hansatech Instruments, Kings Lynn, UK). Leaves were acclimated to the dark using light weight leaf clips for at least 20 min before measurements were taken. Baseline (F0) and maximum (Fm)
fluorescence were measured and variable (Fv = Fm - F0) fluorescence and the ratio of variable fluorescence to maximum fluorescence (Fv/Fm) ratio were calculated from these data. Leaf
absorbance readings were also taken using a chlorophyll meter (SPAD-502; Minolta, Ramsey, NJ) as a nondestructive tool for estimating leaf Chlorophyll (Markwell et al., 1995). SPAD readings were also recorded at 31 July (IP), and 13
September (GF).
Means and standard errors were obtained for each data. Data were subjected to analysis of variance by using a factorial design. All percentage values were transformed using the arcsine transformation before analysis. Where a significant F-test was observed, mean separation among
treatments was obtained by Tukey's test. Data were analyzed using Statistix (version 8.0; Analytical Software, Tallahassee, FL, US).
RESULTS AND DISCUSSION
Photosynthetic rates of two Colombian rice cultivars were examined at two different stages of
crop growth (IP and GF) under short-term high temperature stress and the results are summarized in the Figure 1A and B. Figure 1 shows that significant differences were obtained in the triple interaction cultivar x stage x temperature. Shortterm high temperature stress caused a reduction on the photosynthesis rate in both cultivars either IP or GF phases. At IP stage, 'F50' rice plants showed a higher reduction on photosynthesis net when plants were exposed to high temperatures, as compared to 'F733' (Figure 1A). Heat stress decreased the photosynthesis by~35% in 'F50' rice plants; whereas, CO2 assimilation rate diminished by~20% in 'F733'. At GF stage, Pn was lower than in IP stage in all cases (Figure 1B). Photosynthesis significantly decreased when plants were under high temperatures for five consecutive days in both cultivars. 'F50' and 'F733' rice plants showed a reduction on photosynthesis net by ~38% and ~20%, respectively, as compared to control plants.
Cao et al. (2009) also found that high temperatures during vegetative and early grain filling phases caused a diminishing on photosynthetic rate of flag leaf in different rice's genotypes. Regarding stomatal conductance (gs), similar trends than in measurements of photosynthesis net were obtained (Figure 2).
In general, gs was also seriously affected by short-term high temperature stress. The measurements revealed varietal differences on gs due to two temperature levels in both stages. For instance, when rice plants exposed to high temperature for 5 days, gs decreased by ~20% in cv. Fedearroz 50 and ~ 30% in cv. Fedearroz 733 at IP stage and by ~ 40% in cv. Fedearroz 50 and ~ 50% in cv. Fedearroz 733 at GF phase. A significant correlation between gs and Pn was also obtained for the pooled data from all treatments combinations
(r = 0.86, P < 0.01; Figure 3). Lowest photosynthesis and stomatal conductance data were observed when plants were exposed to short-term heat stress at grain filling stage in both cultivars. We examined the effects of high temperatures on Fv/Fm, SPAD readings, spikelet per panicle and spikelet sterility in two cultivars at two different phenological stages. Table 1 shows that there was not significant differences in triple interaction cultivar x stage x temperature on Fv/Fm, SPAD readings, spikelet per panicle and spikelet sterility. Even though Fv/Fm and SPAD readings were not affected by high temperatures, these variables diminished significantly among phenological phases. The decrease of photosynthesis, stomatal conductance, Fv/Fm, and SPAD values during grain-filling is not surprise since leaf senesce as well as plant canopy was in progress. In contrast to above results, studies performed by Yin et al. (2010) found that Fv/Fm decreased significantly at temperatures higher than 35°C in cv.9311 rice leaves at maximum tillering. Nevertheless, recent works have showed that heat stress does not cause serious PSII
damage, but it can inhibit the repair of PSII (Allakhverdiev et al., 2008, Sharkey, 2005). Despite the fact that differences were not found in the triple interaction on the number of spikelet per panicle, however; there were varietal differences on the number of spikelet per panicle. 'F733' had a higher number than 'F50'. Symptoms of rice grain rot were observed in the heat-treated plants (data not shown). This work showed that the reduced yield characteristics of Colombian cultivars under high temperatures may also be caused by lower photosynthesis rate, since CO2 assimilation rate is vital for yield sustainability because of it contributes in the spikelet number determination (Fageria, 2007; Ishii 1995). Cao et al. (2009) also concluded that the relative high yield in heat-tolerant genotypes of rice is associated with high levels of photosynthesis rate in leaves. In consequence, these results seem to confirm field observations where 'F50' rice plants under heat stress either IP or GF phases have showed a higher susceptibility, causing a lack of response on yield characteristics.
3C
О 25 -
:c
Control
Heat Stress
Control
Heat Stress
Figure 1 Effect of short-term high temperature stress on leaf photosynthesis of cv. Fedearroz 50 (■ F50), and cv. Fedearroz 733 (IZ F733) during initiation of panicle primordial (A) or grain-filling (B) phases. Each bar chart represents the mean of four values. Vertical bars represent ± S.E.
Figure 2 Effect of short-term high temperature stress on stomatal conductance of cv. Fedearroz 50 (■ F50), and cv. Fedearroz 733 (□ F733) during initiation of panicle primordial (A) or grain-filling (B) phases under short-term high temperature stress. Each bar chart represents the mean of four values. Vertical bars represent ± S.E.
26
24
22
o
H 20
o
£
* is
mu
"5
0)
■C
c
</>
*
o
JZ
0.
16
14
12
10
• FKi-IP-Cuntrn-l
0 F50-IF-High TEmpEraturE •
T F7SS-IP-C crtr d
V F/33-IP-H tgh T EmpEraturE
- ■ FM-GF-Contol
□ F50-GF-Hi-gh T Em p e ratu e
F/S3-G F-Conhsl
0 F/BS-GF-High TEmpEraturE ▼
- ■
- V ♦ o
- o □ 1 i i 1
200 400 600 800
Stomatal conductance (mmol CO
1000
Figure 3 Relationship between stomatal conductance and leaf photosynthesis across cultivars and phenological phases. Each point represents the mean of four values.
Table 1. Effects of short-term high temperature stress during initiation of panicle primordial or grain-filling phases on Fv/Fm, SPAD readings, Spikelts per panicle and spikelet sterility of two Colombian rice cultivars (Fedearroz 50 (F50) and Fedearroz 733 (F733))
Cultivar Stage Temperature Fv/Fm SPAD Spikelets per panicle Spikelet sterility (%)
F50 IP Control 0.84 a 40.33 a 291.13 a 43.84 b
Heat stress 0.82 a 42.05 a 297.88 a 42.19 b
GF Control 0.77 b 34.88 b 291.13 a 43.84 b
Heat stress 0.79 b 31.99 c 299.88 a 45.18 b
F733 IP Control 0.84 a 41.45 a 361.25 b 22.15 a
Heat stress 0.82 a 41.15 a 308.00 b 45.68 b
GF Control 0.78 b 34.75 b 361.25 b 22.15 a
Heat stress 0.78 b 33.62 bc 375.88 b 54.53 b
Analysis of variance
Cultivar N.S. N.S. *** N.S.
Stage *** *** N.S. ***
Temperature N.S. N.S. N.S. **
Stage x temp ** ** N.S. N.S.
Stage x cultivar N.S. N.S. N.S. N.S.
Temp x cultivar N.S. N.S. N.S. ***
Cult x Temp x Stage N.S. N.S. N.S. N.S.
C.V. (%) 2.35 4.30 14.83 18.61
Ns, **, and ***, non-significant differences or significant differences at P <0.01, or 0.001, respectively.
In summary, high-temperature stress during the initiation of panicle primordia or grain-filling stages had a profound effect on CO2 assimilation of rice leaves mainly because of low stomatal conductance, especially, in 'F50' rice plants. In addition, the effects of short-term high temperature stress on photosynthesis and grain filling varied among genotypes and the developmental stages of plant when exposed to the stress.
ACKNOWLEDGEMENTS
This research was supported by the Fondo Nacional del Arroz, Colombia. Authors acknowledge Centro Experimental Las Lagunas for technical facilities during the development of this research.
REFERENCES
Allakhverdiev, S.I.; Kreslavski, V.D.; Klimov, V.V.; Los, D.A.; Carpentier, R.; Mohanty, P. (2008) Heat stress: an overview of molecular
responses in photosynthesis. Photosynthesis
Research 98, 541-50 Cao, Y.Y.; Duan, H.; Yang, L.N.; Wang, Z.Q.; Liu, L.J.; Yang, J.C. (2009) Effect of high temperature during heading and early filling on grain yield and physiological characteristics in Indica Rice. Acta Agronomica Sinica 35, 512-521 Castilla, L.A.; Pineda, D.; Ospina, J.; Echeverry, J.; Perafan, R.; Garces, G.; Sierra, J.; Diaz, A. (2010). Cambio climatico y produccion de arroz. Revista Arroz 58, 4-11 Fageria, N.K. (2007) Yield Physiology of Rice. Journal of Plant Nutrition 30, 843-879 Fedearroz. (2011) Durante semestre B-2010 Arroz crece en areas y mejora rendimientos. Revista Arroz 58, 4-9
Jagadish, S.V.K.; Craufurd, P.Q.; Wheeler, T.R. (2007). High temperature stress and spikelet fertility in rice (Oryza sativa L.) Journal of Experimental Botany 58,1627-1635 Ishii, R. (1995) Roles of photosynthesis and
respiration in the yield-determining process.. In Matsuo, T., Kumazawa, K., Ishii, R., Ishihara, K., Hirata, H. (ed.), Science of the rice plant: Physiology, vol. 2. Food and Agriculture Policy Research Center, Tokyo, Japan, pp. 691-696
Markwell, J.; Osterman, J.C.; Mitchell, J.L. (1995). Calibration of the Minolta SPAD-502 leaf chlorophyll meter. Photosynthesis Research 46, 467-472
Sharkey, T.D. (2005). Effects of moderate heat stress on photosynthesis: importance of
thylakoid reactions, rubisco deactivation, reactive oxygen species, and thermotolerance provided by isoprene. Plant Cell and Environment 28, 269-77
Taniyama, T.; Subbaiah, S.V.; Rao, M.L.N.; Ikeda, K. (1988) Cultivation and Ecophysiology of Rice Plants in the Tropics, 3: Photosynthesis of Rice Cultivars of India, Measured by the Tsuno Simple Method. Japanese Journal of Crop Science 57, 184-190 Yang, C.H.; Heilman, J.L. (1993) Response of rice (Oryza sativa L.) to short-term high
temperature: growth, development, and yield. Journal of Agricultural Research of China 42, 1-
11
Yin, Y.; Li, S.; Liao, W.; Lu, Q.; Wen, X.; Lu, C. (2010) Photosystem II photochemistry, photoinhibition, and the xanthophyll cycle in heat-stressed rice leaves. Journal of Plant Physiology 167, 959-966
