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30Methods : Asthmawere revealed in guinea pig by using albuminmethodwith application of injection and atomi-zation inhalation, according to references[1]. After modeling, the model guinea pigs were randomly divided into model group, extract group, extract non - acupoint group, powder acupoint group, powder non - acupoint group. Eight unmodified guinea pigs were used as normal control group.Acupoints choose Ojo, bilateral Feishu and bilateral Shenshu, non-acupoints choose Feishu and Shenshu points next to open 1 cm Department[2]. The patients in the treatment group were treated with 6 hours daily and treated every other day for 7 times. The normal group and the model group were given normal saline.To observe the number of mast cells in BALF[7,8].
Results and discussion
Tab.1 The influence of different kindsG of formsof administration on tthe number of mast cells ( ±s, n=8)
group control
extract -acupoint powder-acupoint
the number of mast cells
degassed percentage (%
15.17±2.32
16.67±2.73##ДД^
22.67±4.37**##ДД
extract-non-acupoint 23.50±4.23**## powder-non-acupoint 35.67±6.95** model 37.67±5.72**
16.48±2.36
18.13±2.90##ДД^
23.53±2.92**##ДД
28.86±7.27**##
40.51±4.65**
43.72±3.21**
Note : Compared with the control , *P<0.05, **P<0.01 ; Compared with the model ,, #P<0.05, ##P<0.01 ; Compared with acupoint with same dosage form, AP<0.05, AAP<0.01 ; Compared with the model , ^P<0.05
= control
■ extract -acupoint S powder-acupoint ■■ extract-non-acupoint B powder-non-acupoint :: model
i
the numberof mastcells degassed percentage (%)
Fig.1 The influence of different kindsG of formsof administration on tthe number of mast cells
It was noted thatthe mast cells morphology of each group are as follows: control( larger and round) ; model( small and rupture) ; powder non-acupuncture points and model group is roughly the same; other groups of the above situation have different degrees of improvement.
■ ■P<0.01
50 -
40 -
30 -
20 -
10 - _
0 -,
The number of mast cells and degassed percentage (%) in BALF was higher than that in the normal group (P <0.01). The number of mast cells in the alveolar lavage fluid of the other treatment group was lower than that of the model group (P <0.01). The above phenomenon was significantly lower in the group (acupoint group and acupoint group) (P <0.01). The above points were lower than those of acupoints (group of ointment and dressing group) (P <0.01).
Our experiment showed that the extract is best, acupuncture better than non-acupuncture points. We speculate that the relative specificity of acupoints may bethe associated substance.
References:
BL] Gao Yunjuan, Ren Yuan, Wu Guotai.Study on the Current Situation of Asthma Animal Models [J]. Chinese Pharmacology and linics, 2012,28 (5): 231-233.
[21Huaxing Bang, Li Lirong, Zhou Haoliang, etc.Development of rat acupoints [J]. Laboratory Animal and Animal Experiment, 1991, (1): 1-5.
[3]CHEN Yi-ping, LI Chao-jun.Study on Establishment and Research of Animal Model of Bronchial Asthma [J] .Journal of Medicine, 2014, 20 (5JT 840-843.
RESEARCH PROGRESS ON DIHYDROQUERCETIN Zhaobin Chen, Bo Zhang, Hongdan Xu, Shuming Huang *
(Heilongjiang University of Chinese medicine, China, Harbin 150040)
Abstract: Dihydroquercetin is an important anti-tumor elements in the treatment of cancer and cardiovascular and cerebrovascular diseaseprevention andtreatment have important applications.This article fromthree aspects ofdihydro quer-cetin is reviewed in detail in this paper. We through the study of structural characteristics dihydro quercetin and its pharma-
о
cological action, to improve the application of dihydroquercetin effect to meet the clinical needs of medical ap-plications. Key words: Dihydroquercetin; Research progress; Outlook; Pharmacological effects
Dihydroquercetin as an important kind of flavonoids compounds exists in various plants, the content is higher in the larch, especially Douglas fir. Nowadays, dihydroquercetin anticancer, because of oxidation, antivirus, resistance to radiation, resistance to diseases of the cardiovascu-lar system, improve microcirculation of capillary, improvement the action such as brain blood circulation, antiplatelet agglomeration. This article emphatically from the structure and function of the second hydrogen quercetin, detection means, the extraction method and application de-velopment and so on four aspects elaborates the research progress on its.
1 Dihydrogen grid structure and function of the skin pigment Dihydro quercetin (dihydroquercetin), alias tax-ifolin (taxifolin), also called taxifolin, Doug-las fir, double hydrogen quercetin or (2R, 3R) - dihydro quercetin. Formula for Ci5H, 207, the relative molecular mass is 304.25, the CAS: 480-18-2, the physical properties: light yellow pow-der, colorless needle crystal (50% ethyl alcohol), melting point is 240 degree. Solvent soluble in ethanol, acetic acid, such as boiling water, slightly soluble in cold water, almost insoluble in ben-zene.
Dihydro quercetin has many important biological active function, and activate a variety of enzymes can be enough to produce different physiological effect. Dev. et al. The study found that 2 hydrogen quercetin can of normal lymphocytes increase produce different degree of inhi-bition. The study found that two hydrogen quercetin can slightly inhibit the growth of p shape cell carcinoma (HTB43), in a certain concentration range gently stop HTB43 cells grow.
2 Detection method The detection method has five methods include, thin layer scanning method; spectrophotometry; high performance liquid chromatography; reversed phase high performance liquid chromatography and the last method is that high performance liquid chromatography - mass spectrum usage.
3 The extraction technology Assassination plum rose extract technology was studied by the method of solvent extrac-tion, dahurian rose fruit rose stalk dihydro quercetin was extracted in research. In the extraction process by orthogonal test to determine dihydro quercetin extracted from dahurian rose fruit rose the optimal conditions for: extraction temperature 30 t, acetone concentration was 70%, and sol-id-liquid ratio is 1:16 and time take 4 h.
4 Summary and Prospect This article from the structure and function of dihydro quercetin, detection method and take the four aspects are detailed described the research progress of extraction technology. Because the second hydrogen quercetin has medicinal properties such as anti-tumor, anti-virus, an-ti-oxidation, so now extensively applied in medicine, health care products, food, agriculture, in-dustry, tobacco industry, such as further depth development dihydro quercetin extraction tech-nology is very important for its development and should be used.
Reference:
[1] Wang X, Meng M, Gao L, et al. Permeation of astilbin and taxifolin in Caco-2 cell and their effects on the P-GP [J]. International Journal of Pharmaceutics, 2009, 378: 1-8.
[2] Ambika Devi M, Das N P. In vitro effects of natural plant polyphenols on the proliferation of normal and abnormal human lymphocytes and their secretions of interleukin-2 [J]. Cancer Lett, 1999, 69 (3): 191-196.
[3] Kawaii S, Tomono Y, Katase E, et al. Effect of citrus flavonoids on HL-60 cell differentiation. Anti-Cancer Res, 1999, 19(2A): 126l-1269.
[4] Kandaswami C, Perkins E, Drzewiecki G, et al. Differential inhibition of proliferation of hu-man squamous cell carcinoma and embryonic fibroblast like lung cells in culture by plant flavo-noids[J]. Anti-Cancer Drugs, 1992, 3(5): 525-530.
[5] Kliukien R, Sarlauskas J, Narimantas C. Inhibition of phthalocyanine sensilized photo hemoly-sis of human erythrocytes poly-phenolic antioxidants: description of quantities structure - activity relationships[J]. Cancer Lett, 2000, 157(1): 39-44.
[6] Yun B S, Lee I K, Kim J P, et al. Lipid peroxidation inhibitory activity of some constituents isolated from the stem bark ofEuca-lyptus globulus[J]. Archives of pharmacal research, 2000, 23(2): 147-150.
Author: CHEN Zhao-bin email: [email protected] HUANG Shu-ming email: [email protected] Address: Hei-longjiang University of Traditional Chinese Medicine, Harbin, Heilongjiang, Heping Road, No.24, 150040
MAIN PHARMACODYNAMIC RESEARCH OF QIXUANYIJIAING ON TREATMENT FOR HYPERTHYROIDISM
ZHEN Zhea, LIU Shu-minb, LIU Chang-fengc*
(a Heilongjiang University of Chinese Medicine, Harbin 150040, China; b Drug Safety Evaluation Center, Heilongjiang University of Chinese Medicine, Harbin 150040, China;c Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China) author at: Heilongjiang University of Traditional Chinese Medicine, He Ping Road 24, Harbin 150040, PR China. Tel: +86 13339522609. E-mail address :870810715 @qq.com.
Abstract Objectives: The purpose was to study and develop the curative effect and mechanism of Qixuanyijiaing on hyperthyroidism. Methods: By dividing 60 SD female rats into control group and model group, hyperthyroidism model rats were copied by injecting Yersinia enterocolitica into rats' caudal vein. The model groups were randomly divided into model group, Qixuanyijianing high, medium, low dose group and positive medicine group respectively. Then indicate the changes in the appearance, weight, serum and thyroid tissue of Qixuanyijiaing-treated group. Results: Qixuanyijiaing could significantly improve the body weight of hyperthyroidism rat, and reduce the levels of serum 3,5,3'—triiodothyronine (T3), thyroxine (T4), increase the levels of thyrotropic-stimulating hormone(TSH), and improve the thyroid tissue pathological changes. Conclusions: The result indicated that Qixuanyijiaing has a good therapeutic effect to hyperthyroidism rat.
Key Words: Qixuanyijianing; hyperthyroidism; Pharmacodynamics Reference
