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Материалы конференции /Proceedings of the Conference/
In the rat, CD4+ T cells can be divided into Thl-like CD45RC+CD4+ T cells, which express IL-2 and IFN-y and play an important role in cell-mediated immune responses, and Th2-like CD45RC-CD4+ T cells, which express IL-4 and IL-10 and play an important part in humoral immune responses. It has been suggested that the immune balance between Th1- and Th2-type cells plays an important role in the maintenance of peripheral tolerance. The dominance of Th1 cells over Th2 cells is associated with the development of autoimmune type 1 diabetes, whereas the dominance of Th2 cells over Th1 cells is associated with the prevention of type 1 diabetes. It was previously found that KRV infection in DR-BB rats increased the expression of Thl-type cytokines in the splenocytes and pancreatic infiltrates. Therefore, it is possible that the proportions of Th1 and Th2 cells are altered during KRV infection in DR-BB rats (Todd J.A., Walker N.M., 2007).
Conclusions. Different experimental models of diabetes give an opportunity to find the new approaches for this disease treatment as well as to prevent complications connected with it.
YAK 616.83-085.21
Skihar V., Silva C., Chojnacki A., Döring A., Stallcup W.B., Weiss S., Yong V.W. Hotchkiss Brain Institute, University of Calgary, Calgary, Canada
PROMOTING OLIGODENDROGENESIS AND MYELIN REPAIR USING THE MULTIPLE
SCLEROSIS MEDICATION GLATIRAMER ACETATE
The formation of oligodendrocytes (oligodendrogene-sis) and myelin is regulated by several neurotrophic factors. Strategies to increase the level of these trophic molecules may facilitate repair in demyelinating conditions, such as multiple sclerosis (MS). Because leukocytes are a source of neurotrophic factors, and as glatiramer acetate (GA) generates T helper 2 (Th2) lymphocytes that are not known to be harmful, we tested the hypothesis that GA regulates oli-godendrogenesis and myelin formation. First, we generated GA-reactive Th2 cells and determined that they produced transcripts for neurotrophic factors, including insulin-like growth factor-1 (IGF-1). The conditioned medium from GA-reactive T cells elevated IGF-1 protein and promoted the formation of oligodendrocyte precursor cells (OPCs) from embryonic brain-derived forebrain cells in culture. We next subjected mice to lysolecithin-induced demyelin-ation of the spinal cord. At 7 days after the insult, the number of OPCs in the demyelinated dorsal column was higher than that in uninjured controls, and was further increased by the daily s.c. injection with GA. Increased OPC generation by GA was associated temporally with the elevation of IGF-1 and brain-derived neurotrophic factor (BDNF) in the spinal cord. Finally, the resultant remyelination at 28 days was higher in mice treated with GA during the first 7
days of injury compared with vehicle controls. These results indicate that GA promotes oligodendrogenesis and remyelination through mechanisms that involve the elevation of growth factors conducive for repair.
УДК 616.43/.45-055.25+618.17 Tsysar Yu.V., Andriiets' O.A.
Department of Obstetrics and Gynecology with the Course of Infantile and Adolescent Gynecology Bukovinian State Medical University, Chernivtsi
REPRODUCTIVE HEALTH OF ADOLESCENT GIRLS WITH ENDOCRINE DISORDERS
Pubertal uterine bleedings are a topical problem of modern adolescent gynecology. As a rule, in case of disturbances of the menstrual cycle in girls in the form of pubertal menorrhagias the hormonal background changes, the synthesis and secretion of the gonadotropic hormones is disturbed, the secretion of estradiol and progesterone changes. Thyroid hormones exert an immediate effect on the organs of the reproductive system, inhibiting the follicle-stimulating function and enhancing the luteinizing function of the hypophysis and in its turn, the sensitivity of the ovaries to the gonadotropic hormones and the endometrium to estrogens increases.
Aim and object of the research. We have studied the concentration of the sex and thyroid hormones of the serum in the blood of teen-age girls, suffering from pubertal menorrhagias against a background of the thyroid gland pathology.
Material and methods of the research. We have examined 70 adolescent girls with pubertal menorrhagias who were treated at the gynecological unit of Municipal Clinical Maternity Hospital № 1 (MCMH № 1) of Chernivtsy City and subdivided into two groups: group I (basic) — 30 teenage girls with the diagnosis of pubertal menorrhagias with underlying concomitant pathology of the thyroid gland, group II (of comparison) — 40 teen-age girls with the diagnosis of pubertal menorrhagias, 27 apparently healthy teen-age girls (the control group).
All the subjects underwent a multimodality hormonal examination with an evaluation of the concentration of hormones in the blood serum by means of the immune enzyme analysis (IEA) method, using the immunoenzymatic microplate semiautomatic analyzer — Expert Plus Asus, (Biochrom Ltd, England) and anassay kit VECTOR Best (Russia). Statistical data processing was carried out by means of a package of computer programs Statistica 6,0. Just this very investigation of sex hormones as estradiol (E2), progesterone (P), follicle-stimulating hormone (FSH), luteinizing hormone (LH) and thyroid hormones — thyroxine (T4), triiodothyronine (T3) and thyrotropin—releasing hormone (TRH) was assumed as the basis for the purpose of studying their concentration in the blood serum in teen-age girls with pubertal menorrhagias with concomitant pathology of the thyroid gland.
№ 6(54) • 2013
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