Научная статья на тему 'Применение фенотипических методов выявления отдельных маркеров резистентности к антибиотикам у микроорганизмов, выделенных из клинического материала'

Применение фенотипических методов выявления отдельных маркеров резистентности к антибиотикам у микроорганизмов, выделенных из клинического материала Текст научной статьи по специальности «Фундаментальная медицина»

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Аннотация научной статьи по фундаментальной медицине, автор научной работы — Андреева Светлана Владимировна, Бахарева Лариса Израильевна, Попова Маргарита Александровна, Шафикова Наиля Эмильевна

Рассмотрена необходимость практического применения специальных методов, позволяющих определить маркеры резистентности к антибиотикам у условнопатогенных микроорганизмов, выделенных из клинического материала. Выявлено, что микроорганизмы, обладающие маркерами резистентности к антибиотикам, могут расцениваться в рутинной практике как чувствительные, что приводит к клинической неэффективности терапии антибактериальными препаратами

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Текст научной работы на тему «Применение фенотипических методов выявления отдельных маркеров резистентности к антибиотикам у микроорганизмов, выделенных из клинического материала»

SUMMARIES

A. L. Burmistrova

THE ANTITUMORAL IMMYNITY. THE MORDERN VIEW ON THE STRESS AND DEATH OF TUMOR CELLS

This review summarize the state-of-the-arts of cancer immunity. Recent advances suggest that apoptosis can be immunogenic. Tumor cells may be «stressed» before death by different signal that lead to their immunogenicity. As the response on chemotherapy or radiotherapy, tumor cells can express «danger» and «eat me» signals on the cell surface or can secrete immnnpgtimiifatnry factors to stimulate immune effectors.

A, V. Korobkin, T. A. Suslova, M, N. Rusakov, /. V. Shmunk, O. V Korobitsyna, M, N. Zaharova, M. O. Kiseleva, M. A. Lyubchenko, Y. A. Markova

THE RESULTS OF ACUTE LEUKEMIA TREATMENT AND ANALYSIS OF MINIMAL RESIDUAL DISEASE (PRELIMINARY DATA)

The molecular genetic analysis in acute leukemia has practical importance for diagnosis confirmation, prognosis evaluation and for monitoring of minimal residual disease to estimate the remission quality and to predict relapse.

I. V. Devoid, E. A. Kkodus, T. A. Suslova, A. L. Burmistrova

INFLUENCE OF GENE HLA DRB1*04 ON OCCURRENCE AND CURRENT OF RHEUMATOID ARTHRITIS

Researched presence of gene HLA DRB1*04 at patients with rheumatoid arthritis in comparison with healthy donors. Following data have been revealed: at patients with rheumatoid arthritis gene HLA DRB1*04 met in 55 %, at healthy donors in 15 %. The interrelation between frequency of revealing a gene and weight of current of disease is revealed: at patients with presence of gene HLA DRB1*04 it was marked higher activity the diseases sick with the given gene in most cases (89 %) were positive on rheumatoid factors that causes heavier current rheumatoid arthritis.

K E Philippova, M. I Ugnivenko, S. S. SHestopalov, A. L. Burmistrova

ANTIBACTERIAL ACTIVITY OF NEUTROPHILS IN «SIRS-I», «SIRS-III» AND SEPSIS BY PATIENTS WITH BURN

In this study, we investigated indexes of haemogramma and antibacterial activity of neutrophils in «SIRS-I», «SIRS-III» and sepsis by burn patients. In patients with «SIRS-I» and «SIRS-III» was shown leucocytosis with immature form cells; common antibacterial potential and intracellular killing activity of neutrophils were the same, as in healthy people. However, leucocytosis, common antibacterial potential and intracellular killing activity of neutrophils in sepsis were lower, than in «SIRS-I» and «SIRS-III». Remarkably, antibacterial activity of neutrophils «in vitro» as regards to E. coli was significantly higher than to St. aureus.

K K Philippova, E. I Lupar, E, P,. Koneva, M. I Ugnivenko, O. A. Strunina,

S. S. SHestopalov, A. Z. Burmistrova ___

ASSOCIATION OF HLA-DRB1 POLYMORPHISMS WITH SYSTEMIC

INFLAMMATORY RESPONSE SYNDROME (SIRS)

The following paper contains a short review of literature about Systemic Inflammatory Response Syndrome as well as the results of study performed in order to find possible association of SIRS with HLA-DRB1 polymorphisms.

a N. Dimcheva, T. A. Suslova, A. L. Burmistrova, S. V Timofeeva, D. S. Stashkevich

THE GENETIC POLYMORFISM OF HLA-DR LOCUS OF THE NAGAIBAK’S ETHNIC GROUP, LIVING AT THE CHELABINSK REGION

This research in a part of scientific on the "Genetic polymorfism of populations of Chelabinsk region” of Scientific and educational Centre of “Molecular immunogenetics” UrD RAS/SEIHPE “Chelabmsk State University” — Chelabinsk Regionals Station of Transfusion/

S. V. Andreeva, L. I. Bakhareva, M. A. Popova, N. E. Shafikova

APPLICATION PHENOTYPE METHODS OF REVEALING SEPARATE MARKER RESISTANCE TO ANTIBIOTICS AT MICROORGANISMS, DEDICATED FROM A CLINICAL MATERIAL

In research necessity of practical application of the special methods has been considered, allowing define pour markers of resistance to antibiotics at the is conditional-pathogenic microorganisms allocated from a clinical material. It is revealed, that the microorganisms possessing markers ofresistency to antibiotics, can be regarded in a routine practice as sensitive, that leads to a clinical inefficiency of the antibacterial therapy.

E. A. Mokrinskaya, L. I. Bakhareva, M. V. Bazhenova, L S. Nikolaeva

ALGORITHM SCREENING INSPECTIONS OF PREGNANT WOMEN ON A TORCH-INFECTION IN MODERN CONDITIONS

727 pregnant women were surveyed by various laboratory methods for acknowledgement etiology TORCH-infections. Installation of ELISA to the definition of the type of antibodies has enabled about other expensive researches- The rational algorithm of inspection of pregnant women with the purpose of revealing of the infectious agent is offered.

L. Zharova, L. I. Bakhareva

APPLICATION OF THE IMMUNE-ENZYME ANALYSIS FOR DIAGNOSTICS CITOMEGALOVIRUS AND HERPES INFECTIONS AT PREGNANT WOMEN

Serums of blood of pregnant women on presence of specific antibodies to CMV (citomega-lovirus) and to a virus of a simple herpes (HSV - herpes simplex virus) I-II type were examined by method ELISA, It is established, that at use of the given method possible to differentiate stage of CMV and HSV infections at pregnant women, and also to predict risk of occurrence of intrauterine infections and various pathologies of a offspring.

j1.1. Bakhareva, A. V. Pospelova, E. N. Kandalova, E, M. Nazarova

ROLE MYCOPLASMA PNEUMONIAE IN OCCURRENCE OF INFECTIONS OF A RESPIRATORY PATH AT ADULTS AND CHILDREN

The material from sick children and adults with the expressed respiratory syndrome with the purpose of acknowledgement of etiologic importance M, pneumoniae has been investigated. In 2213 tests the activator has been found out in 5.2% at use RNL From 597 samples of serum to verify sharp process it was possible in 22,5%. Serological acknowledgement at children it is received more often, than at adults.

A. V. Pospelova, A. L. Burmistrova, S. V. Kvyatkovskaya, K S. Ilomich REVEALING B-INTEGRINS ON A SURFACE OF A CELLULAR WALL OF MUSHROOMS OF CANDIDA SPP. BY FLOWING CITOMEASUREMENT

With the purpose to detection p-integrins on a surface of a cellular wall of Candida spp. fun-gies coloring monoclonal antibodies to CD lib and CD of 21 suspensions of 6 fungies cultures has

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been carried out. Detection of the results was registered by flowing citomeasurement. The given research of a molecule of CD lib and CD 21 on a surface of barmy cells is not revealed.

N. V. Arsenieva, D. U. Nokhrin

HYDROCHEMICAL AND A SANITARY-BACTERIOLOGICAL ESTIMATION OF A CONDITION FL. THE SECOND IN FISH-ECONOMICAL PURPOSES

The sanitary-bacteriological and the hydrochemical research of water fl. The Second has been lead. It is date shown water that fl. The Second concerns to a category of brackish waters, has a chlorinele class, natrium group, type I. On sanitary-bacteriological parameters the reservoir should be carried to the first category (pure). Carried out researches specify conformity of the lake to the veterinarian-sanitary requirements, allowing to conduct on it fish-economical activity.

T. Golovina, O. Kuznetsova

SEPARATING AND IDENTIFICATION OF MUSHROOM MICROFLORA ON DOCUMENTS OF THE UNITED STATE ARCHIVE OF THE CHELYABINSK REGION

The Results of aresearch of the documents from the funds of the State archive of the Chelyabinsk region, were givea This research showed that the documents had been affected by micromycet-es-xylotroph, and also by mushrooms-saprotroph of the mould group. The Representatives of 11 sorts of micromycetes were discovered. One-time processing of documents by «Metatin GTV> is insufficient for utter elimination of the mushroom infection.

S. V Barysheva, G. V. Brukhin

MORPHOFUNCTIONAL FEATURES OF PERITONEAL MACROPHAGES OF ANIMALS WITH EXPERIMENTAL HEPATITIS ...

Ability of peritoneal macrophages to adhesion and its lysosomal and phagocytic activity in animals with experimental D-galactosamine liver injury is studied. Morphofunctional alterations of peritoneal macrophages in experimental animals with liver disorder are established. These changes express in a decrease of lysosome activity, in an ability of macrophages to phagocytosis of Staph, aureus and in a disorder of adhesive properties, which may lead to a disturbance of organism reactivity.

T. I. Biriukova, G. V. Brukhin

MORHPOFUNCTIONAL CHARACTERISTIC MAST CELLS FROM THYMUS OF POSTERITY FROM FEMALE RATS WITH CHRONIC LIVER DESTRUCTION

DIFFERENT GENESIS

Used morphometrical analysis we investigate morhpofunctional features mast cells in thymus

of posterity from rats with experimental liver destruction (D(+) - galactosamme and E. Coli). Detected what experimental posterity have increase quantity of mast cells and increase number

of cells with high secretor activity,

D. R. Zhdanova, O. K Nikolina, K. V. Nikushkina TTvn„™MAT F

MORPHOFUNCTIONAIi CHARACTERISTICS OF THE POSTERITY OF FEMALE

RATS WITH CHRONIC EXPERIMENTAL LIVER INJURY . .

tS Eland morphofunctional features of the posterity of female rate with ohromc expen-

y ^ . , ,• j An inrrease of thyroid gland functional activity as evidenced by

mental liver injury are studied. An increase ot tnyro g lUnmbolodcal chane-

increase of tfayrocy te height and thyrocyte nucleus diameter is established. Morphological chang

es of organ were accompanied by rise of T4 concentration.

0. M. Malisheva, E. N. Pashnina

MAST CELLS ONE OF THE MEMBER MICROENVIRONMENT OF SPLEEN FROM POSTERITY FEMALE RATS WITH EXPERIMENTAL DESTRUCTION OF LIVER

In experimental D-galactosamine destruction of liver we studied quantity and duality features of mast cells from posterity spleen. We detected decreased member of mast cells, increase index of granulation saturation and degranulatoin are sertificate about it.

O. V Nikolina, D. R. Gdanova, A. A.Fedosov, E. N. Pashnina

CHARACTERISTIC PARAPHOLLICULAR CELLS OF THYROID GLAND FROM POSTERITY OF FEMALE RATS WITH CHRONIC NARCOTIC INTOXICATION OF DIFFERENT STAGES OF INVESTIGATION

We studied morhpofunctional development paraphollicular cells (C - cells) thyroid gland from posterity female rats with chronic narcotic intoxication. Paraphollicular cells are detected with immunohistochemistry reaction used streptovidin - biotin method (with monoclonal antibody against calcitonine). Detected that chronic narcotic intoxication female rats are cause of increase quantity paraphollicular cells in thyroid gland.

A. A.Fedosov, S. V. Barysheva, O. V. Nikolina, E. N. Pashnina

CHARACTERISTIC OF THYMUS CELLULAR HOMEOSTASIS OF POSTERITY FEMALE RATS WITH CHRONIC HEROIN INTOXICATION

We studied index of ready to apoptosis and proliferation lymphocytes of thymus and peripheral blood from posterity of female rats with chronic heroin intoxication in different stages of ontogenesis. Proliferation activity is avaluated with Crocker X, Nar P. method (1987) used ozone acid argentums. Ready to Fas-depended apoptosis is detected with CD95 monoclonal antibody in reaction of indirect immunofluorescence. We detected change of quantity «NORs-active» thymocytes and lymphocytes of peripheral blood and decreased their ready for Fas-depended apoptosis. In special series of investigation was done comparative analysis of quantity and functional features of mast cells in thymus of posterity of female rats with chronic heroin intoxication was detected that general quantity of mast cells mid their secretion activity are decreased.

A. S. Romanov

THE CHARACTERISTIC OF MIGRATORY ACTIVITY SPERMATOZOA OF POSTERITY FROM FEMALE RATS WITH EXPERIMENTAL CHRONIC INJURY OF LIVER.

In this study we investigated the migratory activity spermatozoa of posterity from female rats with experimental chronic injury of liver. Our results have demonstrated that posterity from female rats with experimental chronic injury of liver have destruction of «health start» include reproductive health,

G. A. TryapUsina,N.I.Dukhovnaya,,S. P.Belonogova,E, A. Pryakhin THE EFFECTS OF RADIOFREQUENCY ELECTROMAGNETIC RADIATION ON THE HEMATOPOIETIC SYSTEM IN MICE

The effects of 925-MHz GSM electromagnetic field (pulsed at 217 Hz, no voice modulation, power density 1.2 mW/cm2,10 min/day, three days) in mice CBA and white outbred mice were investigated. An increase in the level of bone marrow nucleated cells, in polychromatocytes/normochromatocytes rate, a decrease in the level of the spleen colony forming units detected by the

endotest were revealed. We did not reveal any changes in the number of CFU detected bv the exotest 3

O. S. Kozionova, O. G. Ploshchanskaya, A. V Akleyev, Y. E. Dubrova OPPORTUNITY OF USE OF MINISATELLITE SEQUENCES DNA OF SEXUAL CELLS OF THE PERSON AS MARKER OF ADVERSE INFLUENCE ON A GENOME

Germline mutation rates at eight minisatellite loci (B6.7, CEB1, CEB15, CEB25, CEB 36, MSI, MS31 and MS32) have been studied among the irradiated families from the Techa River population and non-exposed families from the same area. The groups were matched by ethnicity, parental age, occupation and smoking habit. A statistically significant increase in mutation rate was found in the germline of irradiated fathers, whereas maternal germline mutation rate in the exposed families was not elevated. Minisatellite mutation rate in the germline of irradiated fathers did not significantly correlate with the dose-rate at the time of conception. The frequency of minisateUite mutation in the children of irradiated parents did not show any correlation with the doses of in utero exposure to the bone marrow and soft tissues.

S. A. Pushkarev, A. K Akleyev

THE ADAPTIVE CAPACITIES OF PERIPHERAL BLOOD LYMPHOCYTES OF PEOPLE, EXPOSED TO INFLUENCE OF CHRONIC IONIZING RADIATION TREATMENT

The effectiveness of DNA repair and apoptosis mechanisms in peripheral blood lymphocytes of people, affected by chronic radiation exposure, and also capacity of these cells to form an adaptive response was estimated by using neutral variant of comet assay method. The tendency was observed in the experimental group of people, that all comet parameters where lower than in control group. Statistically significant differences between the rates of DNA fragmentation in the samples before acute radiation exposure of the cells, after this exposure and 2 h. later was not seen in the experimental group as well as in the control group. However, the individual cases of an adaptive response and the radiation hypersensitivity was observed in the both groups in these moments of time after irradiation. It was suggested, that an activity of cell protective systems such as DNA repair and apoptosis regulation in the every case depend on individual habits of each of examined human.

E. Blinova, G. Veremeeva

APOPTOSIS OF LYMPHOCYTE PERIPHERAL BLOOD AT CHRONICALLY

IRRADIATED PEOPLE

Published date demonstrating influence of a chronic irradiation of the person on apoptosis of lymphocyte peripheral blood. We used for the detection of initial level apoptosis and level after additional loading are TUNEL with flow cytometry. The increase of an initial level apoptosis and a level apoptosis after 24 hour incubation in group of persons undergone to chronic radiating influence is marked, in comparison with the control.

COMRIRAT^E ^TM^'lON OF<<Tro°INFLlJENCE OF IRRADIATION OF OVARIES AND HYPOTHALAMUS-HYPOPHYSES SYSTEM ON THE PREGNANCY OlOT IN WOMEN EXPOSED ON THE TECHA RIVER

Aim of investigation. To estimate the influence of radiation exposure of ovaries and hypothal-amus-hypophyses system on the pregnancy and delivery outcomes in women exposed to chronic

ir^ectsofresea.hwethewomenlivingondiexechariverside.

The material of the research were 197 questionnaires containing data of special medical (hyneco-logical) inspection.

Results. It was revealed that there is a statistically significant increase of spontaneous abortions and cases of early neonatal mortality frequency with the increase of the ovarial dose and dose to bone surfaces. Besides the level of significance was higher for the dose to bone surfaces. The frequency of preterm labour significantly correlated only with the dose to bone surfaces. Joint frequency of untoward pregnancy outcomes (congenital developmental defects, stillborn children and early neonatal mortality) also showed statistically significant correlation only with the dose to bone surfaces.

I Golovina

BIODIVERSITY AND DISTRIBUTION OF MACROMYCETES OF THE GENUS PHELLINTJS

Some biological features and geographical distribution of genus Phellinus in the world survey and among Russian representative are considered.

V V. Rechkalov, E. V Lebedeva, O. V Golubok

THE DIET OF PERCH IN LAKE SINEGLAZOVO UNDER WINTER ICE

The object of this research is the diet of perch in the shallow lake Sineglazovo during the under ice period, It has been discovered that 4-year-old species chiefly consume plankton crustaceans. Perch of this age group consuming zooplankton was seldom noted, that can be connected both with the complicated alimentary relations within the ichthyocenose and the intensive development of plankton organisms under the conditions of organic pollution.

Jiu P. Gorichev, A. N. Davidichev

CHARACTERISTIC OF THE SEPARATE FOREST TYPES OF THE CONIFEROUS-BROAD-LEAVED FORESTS WITHIN THE SOUTH URALS WEST MACRO SLOPE

Separate results of investigations of the coniferous-broad-leaved forests of the South Urals west macro slope within the South Urals natural reserve has been presented in the paper.

N. M. Tseizer, S. V. Tryapitsyna

ECOLOGICAL EDUCATION SUCCESSION

The common ecological space includes all the stages starting with preschool education and finishing with higher education. It is regulated by federal normative documents. The article analyses the implementation of comprehensive approach. Educational institutions in Chelyabinsk Oblast serve as an example.

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N. I. Khodorovskaya, V. S. Speransky, N. M. Tseizer, S. V. Tryapitsyna, K. S. Chernov

INVENTORIZATION AND RANKING OF SOURCES POLLUTING THE SHERSHNYOVSKOYE RESERVOIR, THE ANALYSIS OF THE EXTENT OF MAN’S IMPACT ON ECOSYSTEM

The data presented in the article are devoted to the results obtained while examining the Shershnyovskoye reservoir’s coastal strip and water-collecting area in the range of the 2nd and 3d ring sanitary control zone. The Shershnyovskoye reservoir is Chelyabinsk’s source of utility and drinking water supply and a place where people rest. The reservoir is exposed to intensive anthropogenic load. This results in deterioration of water quality and reservoir’s ecosystem. The inventarizataon of sources polluting the reservoir has been conducted. The sources have been ranked on the basis of the extent to which they are environmental hazard.

_ K D*pabina> E- V- Safonova, G. O. Bogdanov, S. S. Andreev, V. A. Yachmenev, E. A. Pryahin

IN 2007 BI0L°GICAL ESTIMATION OF SHERSHNEWSKY RESERVOIR’S STATE

The article is connected with the biological assessment of Shershni reservoir condition According to the biological indexes the Shershni reservoir can be characterized as a p-mesosaprobic one with transitional zones in upper course (oligosaprobic zone) and at the dam (a-mesosaprobic zone).

G, O, Bogdanov

TUBIFEX TUBIFEX (TUBIFICIDAE) SURVIVAL AND FERTILITY UNDER LABORATORY KEEPING AND BREEDING

There is no any standard methods for estimate toxicity of sediments using bentic organisms in Russia today. Tubifex tubifex is one of widespread freshwater zoobentic invertebrates. It can be used to create aquaculture for developing test-system for assessment of sediment toxicity. We have developed approaches for longtime cultivation ofTubificidae. During 8 months of cultivation an average fertility of worms was 1,82±0,23 cocoons / individual per 2 weeks. Average survival was 86,7 %. We discovered the variation of reproduction T, tubifex for 8 months. We found high effectivity of I tubifex cultivation at an age of 6-8 months.

E. V. Safonova

THE ASSESSMENT OF DIFFERENT FACTORS INFLUENCE ON CYANOBACTERIAL TOXICITY

Research results on the toxicity of cyanobacterial blooms from the Shershni reservoir (Chelyabinsk region) are presented. In summers of 2005-2007 LD50 of cyanobacteria varied widely (from 48 to 572 mg/kg). The factors influencing the toxicity are analysed. No effect of temperature, year, month, dominant genus on the toxicity is determined. The inverse relation between the total number of cyanobacteria in water and their toxicity is showed. Besides the percentage composition of cyanobacterial genus in bloom is shown to has an influence on the toxicity.

S. G. Levina, A. V. Akleyev, G. G. Korman, L. F Mukhametshina

CERTAIN REGULARITIES OF THE CONTENTS AND ACCUMULATION 90SR AND 137CS IN HIGHER AQUATIC PLANTS OF THE LAKES URUSKUL, LARGE IGHISH AND SMALL IGHISH

The paper summarizes the results from radioecological studies of a number of water basins located on the East-Urals Radioactive Trace (BURT), as exemplified by the lakes Uruskul, Large Ighish and Small Ighish which are included in the medium-distance and near zone of the EURZ The current species composition of the high aquatic plants and species-specific concentration of 90Sr and I37Cs in them has been studied, also, title radionuclide accumulation coefficient have

been estimated.

S. V. Tryapitsyna, A. A. Sebipzyanova ^ ^ _

KEEPING AND DISTRIBUTION OF HEAVY METALS IN TOP-SOIL OF

AGRICULTURES OF SOUTH URAL

There are problems of entrance, accumulation and migration of heavy metals in top-soil on the example of farmings situated in the territory of the South Ural and the influence of physicochemical properties of the soil which are analyzed in the work.

7. A. Kolomiets

THE ASSESSMENT OF GENOTOXIC INFLUENSE OF ELECTROMAGNETIC AGENTS

The aim of this study is to assess die capacity of electromagnetic agents (electromagnetic fields with linear, right, left polarization, frequency 925 MHz and modulation 217 Hz; electric field 217 Hz, magnetic field 217 Hz) to induce genotoxic effects. The mice CBA line were subjected once for 10 minutes to pulse electromagnetic radiation modulated as a GSM standard with the power density 1.2 mW/cm2, electric field 300 W/m, magnetic field 0,7 A/m andy-irradiation, 15 cGr. The polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) in the marrow were examined to determine the extent of micronuclei and PCE/NCE correlations. The electromagnetic exposure significantly increased the frequency of micronucleated PCE and NCE in comparison with sham-irradiated mice. There was no difference in the proportion of PCE/NCE between exposed and unexposed animals.

S. S. Andreev

THE EFFECTS OF EXTREMAL ENVIRONMENTAL FACTORS ON THE AGE-DEPENDENT REACTIONS OF RAT CENTRAL NERVOUS SYSTEM

Work presents the data concerning the assessment of the influence of the radio-frequency electromagnetic radiation on the cognitive function of male rats of different ages. It is shown that the electromagnetic radiation (frequency 925 MHz and modulation 217 Hz, power flux of 1,2 mW/ cm2) does not change the cognitive function.of the rats at die age of 8 months and inproves the cognition function, of the rat at ages of 2 and 18 months.

T. A. Tolmacheva

THE USE OF THE SHF ELECTROMAGNETIC FIELD TO DISINFECT DRIED FRUIT TO REMOVE FUNGAL MICROFLORA

For the first time the article examines the SHF-disinfection method used to remove fungal microflora from raw materials for confectionary and baking industries (dried apricots, prunes, raisins). Patent decision has been obtained. Patent application No. 2003122140/13 (023308). The date of commencement of patent is 15 July 2003.

A. R.Tairovaf R. R.Fatkullin, A. P, Posina

THE VALUE OF SOME FACTORS OF NON-SPECIFIC RESISTANCE OF CATTLE ON THE AREAS POLLUTED BE HEAVY METALS

Beef breed cows in the complex biopathogenic zone of the Southern Urals are noted to have some intensity in the system of natural protection which is accompanied by the decrease of the number of cells responsible for phagocytosis.

R. Sh. Gareev

HOW DAIRY PRODUCTION DEPENDS ON SEASONALITY, WHEN A COW CALVE AND WHEN COW HAS NOT CALVE IN CONDITIONS TROIZKIJ DISTRICT OF CHELYABINSK REGION

We recommend to have the following structure of cows calving according to seasons: autumn calvings — 35 %, winter calvings — 30 %, spring calvings — 20 %, summer calvings —15 % for proportional production of milk throught the year.

B. /. Gumerov

DAIRY PRODUCTION OF COWS FROM DIFFERENT SEASONS OF CALVING

Dairy cow’s production in different seasons of calving, based bn lactation cows of collective farm “Daybreak” is viewing. Uvelsk distrikt of Chekyabinsk Oblast.

D. G KiVver

HOW HEIFER’S AGE OF FIRST SEMINATION INFLUENCE ON DAIRY PRODUCTION

The influence the age of first insemination on the milk productivity was studied. The age of the first insemination of calves influence on their milk productivity. The highest yield was observed in calves inseminated at the age of 15,6-16,5 months it was 10 — 580 kg highes.

D. S, Lazorenko, E. N. Ciulina

DAIRY PRODUCTION OF COWS FROM DIFFERENT TECHNOLOGY OF MILK PRODUCING

The investigations of the influence of milking technology on the farm-useful signs of the black-and-white breed cows were carried out. It was established that the best one is the milking stanchion with milking in the parlor.

A. A. Belookov

HOW TERMS OF KEEPING INFLUENCE ON CALF’S PRODUCTION

At cultivation of younq qrowth of larqe homed livestock the qreat value has a thermal mode in premises as it influences physioloqical functions, health, qrowth and development of animals.

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