CC BY
8
Vestnik FEB RAS. 2018. № 6 Supplement
UDC 615.37:577.114 DOI: 10.25808/08697698.2018.202.6S.048
O.V. CHERNIKOV, H.W. CHIU, L.H. LI, V.I. MOLCHANOVA, IV. CHIKALOVETS, K.F. HUA
Polysaccharide from Pseudopterogorgia americana modulates immune response in macrophages
Key words: macrophages, toll-like receptor 4, immune modulator
The marine polysaccharides have a profound impact on the regulation of immune response. However, the mechanism of immune modulation by polysaccharides isolated from the coral Pseudopterogorgia americana (PPA) in mammalian cells remains unclear. Here, we demonstrated that PPA activated macrophages through toll-like receptor 4.PPA induced the expression ofTNF-a, IL-6 and COX-2 in mouse macrophages, but had no effect on the expression of induce NO and iNOS. The PPA-mediated macrophages activation was regulated by ROS, MAPK, PKC and NF-kB. Notably, PPA pretreatment resulted in a reduced expression of TNF-a and IL-6 in LPS-activated macrophages through the downregulation of IRAK2 expression, MAPK phosphorylation and NF-kB activation, indicating that PPA induced LPS tolerance in macrophages. Taken together, our data indicate that PPA has the potential to be used as an immune modulator in mammals.
* CHERNIKOV Oleg Viktorovich - PhD, The Head of The Laboratory, MOLCHANOVA Valentina Ilinichna - PhD, Senior Researcher, CHIKALOVETS Irina Vladimirovna - PhD, Senior Researcher (G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok, Russia); CHIU Hsiao-Wen (Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan; Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan), LI Lan-Hui (Department of Laboratory Medicine, Linsen, Chinese Medicine and Kunming Branch, Taipei City Hospital, Taipei, Taiwan), *HUA Kuo-Feng (Department of Biotechnology and Animal Science, National Ilan University, Ilan, Taiwan; Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung, Taiwan). *E-mail: kfhua@niu.edu.tw
These authors contributed equally to this work.
