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Abstracts. PHYTOPHARM 2017
Acknowledgement: This work was supported by Ente Cassa di Risparmio Firenze, 2015.
References:
1. Bilia AR, Piazzini V, Guccione C, Risaliti L, Asprea M, Capecchi G, Bergonzi MC. 2017. Planta Med. 83(05): 366-381.
2. Bilia AR, Bergonzi MC. Editorial. Planta Med. 2017, 83(05):365.
3. Bilia AR, Guccione C, Isacchi B, Righeschi C, Firen-zuoli F, Bergonzi MC. 2014. Evid Based Complement Alternat Med. 2014:651593.
4. Piazzini V, Rosseti C, Bigagli E, Luceri C, Bilia AR, Bergonzi MC. 2017. Planta Med. May 4. doi: 10.1055/ s-0043-110052.
PHYTOCHEMICAL EVALUATION OF THYMUS LONGICAULIS IN COMPARISON WITH THYMUS VULGARIS
© Marija Kindl1, Franz Bucar2, Biljana Blazekovic1, Maja Bival Stefan1, Sanda Vladimir-Knezevic1
1 University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmacognosy, Zagreb, Croatia;
2 Karl-Franzens-University, Institute of Pharmaceutical Sciences, Department of Pharmacognosy, Graz, Austria
Thymus longicaulis C. Presl (Lamiaceae) is a small aromatic perennial herb and a typical representative of the Illyric-Mediterranean flora. It is a traditional remedy for cold, flu, cough, nephritis and abdominal pain
[1]. This study was aimed to evaluate phytochemical composition of the aerial parts of T. longicaulis wild-growing in Croatia. In order to find the possibility of its use in modern phytotherapy, the results were compared with those obtained for T. vulgaris, as the officially recognised medicinal plant within the genus Thymus
[2]. The presence of phenolic acids and flavonoids in the methanolic extracts and terpenes from the essential oil was detected by TLC. Chromatographic profiles were established for the studied plant extracts by LC-ESI-MS/MS and RP-HPLC-DAD analysis. All detected phenolic acids belonged to derivatives of rosmarinic acid, while all identified flavonoids were flavons. Rosmarinic acid was found to be the most abundant polyphenolic constituent (48 mg/g) while among flavonoids luteolin-7-O-glucuronide was present with
the highest concentration (14 mg/g). The extraction yield (V/m) of essential oil isolated by hydrodistillation was 1.7%. According to GC-MS analysis forty three compounds were identified, representing 99.7% of the total oil. The essential oil was characterised by a high percentage of the monoterpene fraction (95%) with a prevalence of oxygenated monoterpenes (61%). Thymol (43%), p-cymene (18%) and y-terpinene (11%) were identified as the most abundant compounds. Owing to its phytochemical composition comparable to those of T. vulgaris, this medicinal plant could be considered as a new source of the pharmacopoeial herbal drugs Thymi herba and Thymi aetheroleum.
References:
1. Vladimir-Knezevic S, Kosalec I, Babac M, Petrovic M, Ralic J, Matica B, Blazekovic B. 2012. Cent Eur J Biol. 7:1109-1115.
2. European Pharmacopoeia, 9th ed., Council of Europe: Strasbourg, France, 2017:1538-1541.
EVALUATION OF IN VITRO SYNERGY BETWEEN TANREQING INJECTION AND ANTIBIOTICS AGAINST METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS
© Wei Feng Yang1, Jue Ling Liu1, Biljana Blazekovic2, Ya Nan Sun1, Shu Hua Ma1, Chuan Yun Ren3, Sanda Vladimir-Knezevic2, Yi Wang 1
1 China Academy of Chinese Medical Sciences, Experimental Research Center, Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Beijing, China;
2 University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Pharmacognosy, Zagreb, Croatia;
3 Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, China
In light of the emergence and spread of antibiotic resistance and lack of new drugs, herbal drug and antibiotic combination may represent an interesting therapy option for treat multidrug resistant bacteria. The
Tanreqing injection is Chinese herbal preparation made from Scutellariae radix (Huang Qin), Ursi fel (Xiong Dan), Naemorhedi cornu (Shan Yang Jiao), Lonicerae flos (Jin Yin Hua) and Forsythiae fructus (Lian Qiao).
Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] vol. 15/2017/suppLement 1
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