CC BY
16B-PS-5
Photothermal effect of gold nanoparticles in various modifications and infrared (808 nm) laser radiation on S. aureus
E. Tuchina1, V. Tuchin2
1Saratov State University, Biological, Saratov, Russian Federation 2Saratov State University, Physics, Saratov, Russian Federation
Gold nanoparticles (GNPs), due to the high variety of possible forms, tunable physical properties, including the absorption wavelength in the plasmon resonance region, are of great interest to researchers and practitioners in solving problems of microbiology and ecology. GNPs can act not only as independent active agents, but also as intermediaries for targeted delivery of drugs and active molecules, as well as to combine these two properties [1-4]. A large number of studies are devoted to the photothermal effects of gold nanoparticles in combination with laser radiation on microorganisms. The result depends on the shape and optical properties of the nanoparticles, their functionalization and the parameters of the selected radiation [1-7].
In our previous studies, it was shown that GNP in combination with red (625 nm) or infrared (IR, 805 nm) radiation leads to a reduction in the number of such microorganisms as S. epidermidis and S. aureus by 80% [8]. At the same time, there was a slight increase in the total temperature of the solutions (by 3-4°C). In another work devoted to the study of the functionalized by immunoglobulins of gold nanostretch in combination with laser (808 nm) radiation for staphylococci, the temperature of the medium was 12-15°C, and the death of bacterial populations reached 97% [9].
In this study the effect of infrared laser radiation (808 nm) of different fluence rates on the bacteria Staphylococcus aureus209 P, incubated in solutions of gold nanocubes, nanorods and on glass substrates with fixed nanodiscs, was studied. Radiation with a power density of 60 mW/cm2in combination with nanocubes caused the death of 50% of the bacterial population after 30 min of exposure, in combination with nanostructures - 56%. An increase in the temperature of suspended matter after irradiation was found of no more than 5-6°C. Radiation with a power density of 400 mW/cm2caused a pronounced inhibition of the viability of bacterial cells -by 81% after 30 min. Incubation of microorganism suspensions on the surface of glass substrate containing gold nanodiscs during irradiation (808 nm, 400 mW/cm2) resulted in 99% of bacterial cell death.
References
[1] Huang Y.Y., Sharma S.K., Carroll J., Hamblin M.R, Biphasic Dose Response Low Level Light Therapy - An Update // Response. - 2011. Vol. 9. - P. 602-618.
[2] Yin R., Agrawa T., Khan U., Gupta G.K., Rai V., Huang Y.-Y., Hamblin M.R. Antimicrobial photodynamic inactivation in nanomedicine: small light strides against bad bugs, // Nanomedicine (Lond). - 2015. Vol. 10(15). - P. 2379-2404.
[3] Hamblin M.R. Antimicrobial photodynamic inactivation: a bright new technique to kill resistant microbes // Current Opinion in Microbiology. - 2016. - Vol. 33- P. 67-73.
[4] Karimi M., Zangabad P. S., Ghasemi A., Amiri M., Bahrami M., Malekzad H., Asl H.G., Mahdieh Z., Bozorgomid M., Ghasemi A., Reza M., Boyuk R.T., Hamblin M.R. Temperature-Responsive Smart Nanocarriers for Delivery of Therapeutic Agents: Applications and Recent Advances // ACS Appl. Mater. Interfaces. - 2016. - Vol. 8. - P. 21107-21133.
[5] Wainwright M., Maish T., Nonell S., Plaetzer K., Almeida A., Tegos G., Hamblin M. R. Photoantimicrobials-are we afraid of the light? // The Lancet Infectious Disease. - 2017. - Vol. 17(2). - P. 49-55.
[6] Hamblin M.R. Mechanisms and applications of the anti-inflammatory effects of photobiomodulation // AIMS Biophys. - 2017. - Vol. 4. - P. 337-361.
[7] Tsai S.R., Hamblin M.R. Biological effects and medical applications of infrared radiation // J. Photochem. Photobiol. B. - 2017. - Vol. 170. - P. 197-207.
[8] Tuchina E.S., Ratto F., Khlebtsov B.N., Centi S., Matteini P., Rossi F., Fusi F., Khlebtsov N.G., Pini R., Tuchin V.V. Combined near infrared photothermolysis and photodynamic therapy by association of gold nanoparticles and an organic dye // Proc. of SPIE: Plasmonics in Biology and Medicine VIII. -2011. Vol. 7911. - P. 79111C-1-7.
[9] Tuchina E.S., Petrov P.O., Ratto F., Centi S., Pini R., Tuchin V.V. The action of NIR (808nm) laser radiation and gold nanorods labeled with IgA and IgG human antibodies on methicillin-resistant and methicillin sensitive strains of Staphylococcus aureus// Proc. SPIE 9324: Biophotonics and Immune Responses X. - 2015. - Vol. 93240X. - P. 1-10.
