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DOI https://doi.org/10.18551/rjoas.2020-10.14
PHENOTYPIC PERFORMANCES AND GENETIC PARAMETERS OF QUANTITATIVE CHARACTERS OF BLACK RICE LINES RESULTED FROM PEDIGREE SELECTION
Aryana I.G.P.M., Sudharmawan A.A.K., Aryani I.G.A.U., Ernawati N.M.L.,
Santoso B.B., Wangiyana W.*
Faculty of Agriculture, University of Mataram, Mataram, Indonesia *E-mail: [email protected]
ABSTRACT
This study aimed to determine phenotypic performance, heritability value, genetic diversity, and genetic advance of black rice lines resulted from Pedigree selection. The field experiment was carried out on an irrigated ricefield in the experimental farm of the Faculty of Agriculture, University of Mataram, located in Nyiurlembang Village, West Lombok, Indonesia, from June to November 2019. The experiment was designed according to Randomized Complete Block Design, with three blocks and 25 treatments consisting of 23 black rice lines (G1 to G23) and two parent varieties, i.e. the white rice variety "Situ Patenggang" (G24) and the black rice variety "Baas Selem" (G25). The results indicated that G1, G2, G8, and G20 were promising lines having high grain yields (7-8 t/ha) with long panicles, large number of filled grains, high weight of 100 grains, and high grain yield per clump. They also showed high values of heritability on days to harvest, plant height, number of productive tillers, panicle length, and number of filled grain per panicle; high values of genetic diversity on number of productive tillers, number of filled and unfilled grains per panicle, and grain yield per clump; and a high value of genetic advance on number of filled grains per panicle.
KEY WORDS
Genetics, grains, heritability, productivity, selection.
Rice (Oryza sativa L.) is one of the most important food sources needed by the majority of the world's population, especially in Indonesia. Based on the color of the rice, there are white rice, red rice and black rice. Black rice is functional rice that contains the highest concentration of anthocyan pigment compared with rice of other colors. Black rice could increase body resistance to disease, repair liver damage, reduce cholesterol in the blood, prevent kidney function disorders, and prevent cancer or tumors [1], [2].
The existence of black rice is increasingly rare because it is dominated by the cultivation of high-yielding varieties of modern, ideal, and white hybrids. There are several characteristics of black rice plants that make farmers unlike to cultivate them, which include high plant habitus, long days to harvest and low yield potential [3]. To improve the characteristics of black ice ("Baas Selem") towards ideal plant height, high productivity, large grain size, and early maturity, single cross (followed with Pedigree selection) with the white rice variety "Situ Patenggang", an early maturity and high yielding variety, has been carried out [4].
Pedigree selection is a selection method in segregated populations resulted from cross-breeding processes. This selection is carried out on self-pollinating plants with the aim of obtaining new varieties by combining the desired genes from two or more genotypes. Through this selection, there is an increase in the gene frequency of the desired alleles so that there is a shift in the mean population value to a better direction. This selection is applied if the selected traits have high heritability values on such a character as harvest age, plant height, and others, while quantitative characters such as crop yields generally have low heritability values, so pedigree selection is less effective [5], [6].
The lines resulted from the pedigree selection need to be studied for their phenotypic performance and genetic parameters. The phenotypic performance of a plant can be assessed based on its morphological characteristics. Assessment of these traits can be done
by utilizing a variety of phenotypes in these plants. The diversity of phenotypic characters is the diversity of the appearance of plant traits resulted from an interaction between genetic and environmental factors [7].
The chances to produce new high yielding varieties are greater if the phenotypic diversity is high. The phenotypes expected in rice plants vary but are focused on days to flowering, low plant height, panicle length, number of filled grains, 1000 grain weight and yield potential [8].
Genetic parameters, consisting of genetic diversity, heritability and genetic advance, are essential measures for the success in applying a plant breeding program [9]. Genetic diversity can increase the chances of getting a better genotype through selection. Hetability is a genetic parameter that shows the proportion of genetic relative to phenotype variances of a variety, which indicates if the traits can be inherited in the next generation [6]. According to Acquaah [9] heritability determines the progress of selection, i.e. the greater the heritability value, the greater the selection progress that is achieved and the faster the superior varieties can be released, or vice versa.
This study aimed to determine the phenotypic performance, the values of genetic diversity, heritability, and genetic advance in the pedigree-selected black rice lines.
MATERIALS AND METHODS OF RESEARCH
The experiment was carried out in a technically irrigated paddyfield located in Nyiurlembang Village of Narmada District, West Lombok (Indonesia), from June to November, 2019. The field experiment was arranged based on a Randomized Complete Block Design, with two blocks (replications) and 25 treatments, consisting of 23 black rice lines (G1 through G23), and their parent genotypes, i.e. the white rice variety "Situ Patenggang" (G24) and the black rice variety "Baas Selem" (G25).
The seedlings of 20 days old were transplanted onto the experimental plots with spacing of 25 cm x 25 cm. Basic fertilization was done using 300 kg/ha Ponska (NPK 15-115) 7 days after transplanting (DAP). The next fertilizion was done at 30 DAP using 100 kg/ha Urea, followed by another fertilization at 50 DAP using 100 kg/ha Urea. Other crop maintenance was done based on the optimum cultivation of rice.
Variables observed were days to harvest, plant height, panicle length, number of productive tillers, number of non-productive tillers, number of filled and unfilled grains per panicle, weight of 100 grains, grain yield per clump, and per ha. Data were analyzed with analysis of variance (ANOVA) using SAS and Duncan Multiple Range Test at 5% level of significance for mean differences. The coefficient of genetic diversity (KKG) was calculated according to Syukur et al. [6], heritability in broad sense was calculated according to the formula used by Aryana [10], and Expected Genetic Progress (KGH) was calculated according to the formula used by Syukur et al. [6].
RESULTS AND DISCUSSION
Growth and yield components. The results of ANOVA on the variables measured showed that there were significant differences between genotypes in days to flowering, days to harvest, plant height, number of productive tillers per clump, panicle length, number of filled grains per panicle, weight of 100 grains, grain weight per clump, and grain yield per ha, but not on the number of non-productive tillers per clump, and the number of unfilled grains per panicle (Table 1).
The genotypes having the ealiest maturity were G21, G22, and Situ Patengang (G24), i.e. 112 days after sowing (DAS), while the lines of G1, G2, and G13 were the latest maturity of 118 DAS. Based on the classification of maturity dates from IRRI [11], all lines tested here were under the criteria of early maturity (105-124 DAS). However, according to Widyayanti et al. [12], the longer the life cycle of a plant, the greater the production of biomass and crop yields, as can be seen from Table 3 that G1 and G1 showed the highest grain yield and the
latest maturity (Table 2), but in contrast G4 with the second latest maturity showed the lowest grain yield (Table 3).
Table 1 - Summary of ANOVA results on the variables observed
No. Variables F calc. Probability Significance
1 Days to harvest 2.87 0.006 S
2 Plant height 11.9 0.000 S
3 Number of productive tiller per clump 3.01 0.004 S
4 Number of non-productive tiller per clump 0.72 0.779 NS
5 Panicle length 3.25 0.002 S
6 Number of filled grains per panicle 4.54 0.002 S
7 Number of unfilled grains per panicle 0.58 0.903 NS
8 Weight of 100 grains 4.04 0.000 S
9 Grain weight per clump 2.06 0.040 S
10 Yield (dry grain weight) 2.04 0.043 S
Table F at a = 0.05 = 1.98
Note: S = Significant; NS = Non Significant.
The tallest was G20 with plant height 136.2 cm while Situ Patengang (G24) was the shortest plant (106.8 cm). Based on the plant height category of IRRI [11], the parents, i.e. Situ Patenggang (G24) and Baas Selem (G25) were still in the category of short height (<110 cm), while G7, G8, G17, G18, G19 and G20 were in the tall category (>130 cm ), and other lines were in the medium category (110-130 cm). In terms of the number of productive tillers per clump, G10 (17.3 tillers) was the highest, whereas Situ Patenggang (G24) produced the least number of productive tillers per clumps (10.1 tillers). Unfortunately, although G10 produced the highest number of productive tillers, its grain yield was much lower than in G2 having almost the same number of productive tillers as that of G10. The low yield obtained by the G10 was most probably due to low panicle length and the low number of filled grains (Table 3).
Table 2 - Mean values of harvest age, plant height, number of productive tillers, and number of nonproductive tillers of black rice genotype
Lines Days to harvest Plant height Productive tiller number Non-productive tiller number
(genotypes) (DAS) (cm) per clump per clump
G1 118.0 a1) 124.8 cd 12.2 cdefg 0.0
G2 118.0 a 129.8 abc 10.9 efg 0.0
G3 117.5 ab 132.0 abc 13.3 bcdefg 0.0
G4 117.5 ab 120.0 def 14.1 abcde 0.0
G5 116.0 abc 113.6 fg 10.3 fg 0.1
G6 116.0 abc 120.3 def 11.2 defg 0.0
G7 117.5 ab 135.2 ab 13.1 bcdefg 0.0
G8 116.0 abc 132.6 abc 14.7 abcd 0.0
G9 114.5 abcd 112.8 fg 16.3 ab 0.3
G10 114.5 abcd 107.5 g 17.3 a 0.2
G11 114.5 abcd 107.8 g 14.4 abcde 0.1
G12 114.0 bcd 115.4 efg 15.9 ab 0.0
G13 118.0 a 112.2 fg 16.2 ab 0.3
G14 116.0 abc 120.4 def 13.8 abcdef 0.1
G15 113.0 cd 119.4 def 14.0 abcde 0.2
G16 115.5 abcd 128.6 abcd 13.6 bcdefg 0.2
G17 115.5 abcd 132.5 abc 13.3 bcdefg 0.1
G18 115.5 abcd 134.4 ab 13.8 abcdef 0.0
G19 115.5 abcd 132.8 abc 13.5 bcdefg 0.0
G20 113.0 cd 136.2 a 15.2 abc 0.0
G21 112.0 d 126.4 bcd 13.2 bcdefg 0.2
G22 112.0 d 123.6 cde 13.6 bcdefg 0.2
G23 117.5 ab 119.9 def 15.5 abc 0.0
G24 112.0 d 106.8 g 10.1 g 0.1
G25 116.0 abc 108.9 g 14.3 abcde 0.0
Note:1 values followed by the same letter are not significantly different based on 5% DMRT test; DAS= days after
seeding.
The longest panicle length was in the G21 (28.8 cm) while Baas Selem (G25) produced the shortest panicle (23.6 cm). Based on panicle length classification [13], all the genotypes tested were in the category of medium panicle length (20-30 cm). Plants that have a long panicle will produce more grains so that grain weight per clump and yield per ha could also be higher, as can be seen in G2. However, G22 with almost the longest panicle in fact produced the second lowest grain yield per ha (Table 3).
The highest number of filled grains per panicle was found in Situ Patengang (G24), which was 248.3 grains per panicle, while Baas Salem (G25) produced the least amount (101.2 grains). In general, the number of grains was closely related to panicle length [14]; the longer the panicle, then the greater number of grains per panicle. However, G21 that has a long panicle did not produce a large number of filled grains per panicle. In contrast, the number of unfilled grains per panicle was no significantly different between genotypes.
In contrast, weight of 100 grains and grain yield per clump or per ha were significantly different between genotypes tested. The highest weight of 100 grains was in G10 (3.24 g), while the lowest weight was in G18 (2.71 g). However, higher weight of 100 grains does not always mean higher grain yield, as can be seen from Table 3, that G10, G9, and G11 had the highest weight of 100 grains among the lines, but had lower yields compared with G18 which had the lowest weight of 100 grains. According to Fadjry et al. [15], measuring the weight of 100 grains is useful in determining grain size of a variety, because heavier 100 grains of a variety indicates larger grain size.
Table 3 - Mean values of panicle length, number of filled grains per panicle, number of empty grains per panicle, weight of 100 grains, grain weight per clump, yield of dry grains (ton/ha) in black rice lines
Lines/ Panicle length Filled grain number Unfilled grain Weight of 100 Grain yield per Grain yield
genotype (cm) per panicle number per panicle grain (g) clump (g) (ton/ha)
G1 26.6abcd 206.5ab 20.5 3.04bcde 45.51abcd 7.78a1)
G2 26.9ab 199.3ab 15.1 3.05bcd 40.54abcd 7.61ab
G3 26.1bcde 176.2bcde 17.5 2.97cde 48.58abcd 6.90abcd
G4 24.3cdef 142.1defg 13.7 2.89defg 45.76abcd 3.99f
G5 25.2bcdef 178.6bcde 12.3 2.95cdef 30.29abcd 5.32bcdef
G6 26.7abc 159.4bcdef 24.5 2.89defg 36.34cd 5.05cdef
G7 25.0bcdef 196.2bc 17.5 2.90defg 50.05abc 5.00cdef
G8 25.1bcdef 207.6ab 17.8 2.83efg 57.22a 7.29abc
G9 25.6bcdef 146.1cdefg 16.4 3.21ab 41.41abcd 5.70abcdef
G10 24.7bcdef 110.8fg 19.3 3.24a 32.84d 5.53abcdef
G11 25.8bcdef 162.6bcde 14.8 3.10abc 32.92d 5.43abcdef
G12 26.0bcde 159.9bcdef 18.1 2.93cdef 39.71 bcd 5.33abcdef
G13 26.0bcde 165.0bcde 17.0 3.03bcde 39.60bcd 5.68abcdef
G14 24.6bcdef 132.5efg 14.3 3.00cde 49.69abcd 5.97abcdef
G15 24.5bcdef 171.4bcde 15.0 2.96cde 50.23abc 5.32abcdef
G16 25.8bcdef 201.3ab 18.5 2.75fg 49.24abcd 6.12abcdef
G17 25.0bcdef 190.3bcd 17.2 2.86defg 53.34abc 5.90abcdef
G18 25.3bcdef 201.5ab 22.7 2.71g 53.12abc 6.63abcd
G19 23.9ef 166.3bcde 13.0 2.90defg 54.38ab 6.27abcdef
G20 24.3def 187.9bcd 16.8 2.88defg 54.85ab 7.07abcd
G21 28.8a 162.3bcde 16.7 3.02bcde 48.75abcd 6.87abcd
G22 28.4a 166.4bcde 20.0 3.03bcde 45.72abcd 4.18ef
G23 26.1bcde 188.3bcd 14.0 3.00cde 54.16ab 6.86abcd
G24 25.3bcdef 248.3a 11.9 3.00cde 41.74abcd 4.72def
G25 23.6f 101.2g 11.3 3.02bcde 38.12bcd 6.45abcde
Note:1 values followed by the same letter are not significantly different based on 5% DMRT test.
In relation to grain yield, the highest yield per ha was in G1 (7.78 t/ha) but it was not significantly different from that of Baas Selem or G25 (6.45 ton/ha) and other tested lines except G4 (3.99 t/ha), G5 (5.32 t/ha), G6 (5.05 t/ha), G7 (5.00 t/ha), G22 (4.18 t/ha), and Situ Patengang (G24) (4.72 t/ha). According to Aryana et al. [16], high yields in rice could be influenced by yield components such as number of productive tillers per clump, panicle length, number of filled grains per panicle, weight of 100 grains, and plant population per ha. The high yield was obtained on G1, G2, G8, and G20. It was due to having high yield components such as long panicles, large number of filled grains, weight of 100 grains and high grain weight per clump (Table 4). Based on those yield components, G1, G2, G8, and
G20 produced higher yields than their two parents, so that they are can be examined further to develop new varieties.
Heritability, genetic diversity and genetic advance. Syukur et al. [6] grouped heritability values in broad sense into three classes, namely high heritability values if the value is higher than 0.5; moderate heritability if the value is between 0.2 and 0.5, and low heritability if it is less than 0.2. Based on these, days to harvest, plant height, number of productive tillers, panicle length, and number of filled grains per panicle were in the chategory of high heritability (Table 4). High heritability values of plant height, number of productive tillers, panicle length, and number of filled grains per panicle were also found in the study of Kiani et al. [17], indicating that those traits were more influenced by genetic factors.
The characters showing moderate heritability values were weight of 100 grains, grain weight per clump and grain yield per ha (Table 4), which means that the performance of those black rice lines was controlled by genetic and environmental factors. This means that if the heritability is moderate then genetic advance cannot be obtained in that trait because the observed diversity is the influence of the environments and the dominant phenotypes [18].
The plant traits with low heritability values such as the number of non-productive tillers and the number of unfilled grains per clump (Table 4) indicated that the influence the environmental was greater than the genetic factors. This was caused by a deviation in a generation that allows some individual plants to show certain characteristics that are different from other plants. This deviation was thought to be influenced by environmental factors, such as pest attacks when the rice grain is in milk maturity stages; and it could be also affected by gene action that have an impact on the inheritance patterns [10]. Sutaryo and Sudaryono [19] stated that in characters with low heritability, selection was less effective because the appearance of plant phenotypes was more influenced by environmental factors than genetic factors.
The value of heritability has implications for the efficiency of selection and the selection method to be used. Selection efficiency is determined by the selection character used, where the selection character is chosen based on high heritability values [20]. In relation to selection, if the heritability value is high, the selection method used is the pedigree selection method, while if the heritability is low to moderate, then the selection method used is the bulk method. Heritability also determines progress of selection, the greater the value of heritability the greater the value of the selection progress achieved and the faster the superior varieties can be released or vice verse [21].
Table 4 - Values of heritability in broad sense, genetic diversity and genetic advance for the quantitative characters of black rice lines
Characters Heritability Criteria Genetic diversity Criteria Genetic advance Criteria
Days to harvest 0.61 High 1.55 Low 1.70 Low
Plant height 0.80 High 6.95 Rather low 8.69 Medium
Non-productive tiller number per clump 0.00 Low 4.69 Rather low 0.16 Low
Productive tiller number per clump 0.56 High 11.25 High 11.75 Medium
Panicle Length 0.68 High 5.77 Rather low 6.66 Low
Filled grain number per panicle 0.56 High 15.48 High 16.28 High
Unfilled grain number per panicle 0.12 Low 12.93 High 6.30 Low
Weight of 100 grains 0.49 Medium 3.62 Low 3.56 Low
Weight of grain per clumps 0.42 Medium 11.80 High 10.72 Medium
Grain yield per ha 0.35 Medium 10.31 Rather high 10.73 Medium
The genetic diversity coefficient is classified into 4 groups: the genetic diversity coefficient is high, if the value between 11.61-15.48; the genetic diversity coefficient is high enough if the value between 7.74-11.61; and the genetic diversity coefficient is rather low if the value between 3, 87-7.74; and the coefficient of genetic diversity is low if the value between 0 - 3.87 [6]. In our study, the high coefficient of genetic diversity was found in the number of productive tillers, the number of filled and unfilled grains, and the weight of grains per clump (Table 4). The same investigation was also found in Zen's study [22], which showed that the high coefficient of genetic diversity was found in the productive tiller per clump, the number of filled grains, and the grain weight per clump.
High genetic diversity indicates that the traits tend to be influenced by many genes. Each gene contributes little to the phenotype and is cumulative, but the quantitative trait can also be influenced by environmental changes [23]. Characters with high genetic diversity value have the opportunity to be improved through selection, namely by giving flexibility in choosing the selected genotypes. The high coefficient of genetic diversity indicates a big influence of genetic factors on the appearance [19].
From Table 4 it can be seen that the coefficient of genetic diversity was found rather low in plant height, number of non-productive tillers, and panicle length. The coefficient value of genetic diversity was low found in the characters of harvest age and weight of 100 grains. A trait that has a very high and high genetic diversity value indicates that improvement by selection is possible for this trait. Traits that have high and very high diversity values could be said to have broad genetic diversity, and vice versa, traits that have low and medium diversity values could be said to have narrow genetic diversity. The higher the genetic diversity, the greater the chances of success for breeding programs. In addition, high diversity could also increase selection responses because selection responses are directly proportional to genetic diversity [3].
In our study, it was also found that the number of filled grain has a high heritability and high genetic advance. The estimated value of high heritability is not always followed by a high value of genetic advance. It depends on the selection intensity (k) and additive variance. The estimated value of high heritability and high genetic advance was usually used for the selection of superior strains [24], [18]. In this study, the estimated value of heritability and the high value of genetic advance are found in the number of filled grain (Table 4). This indicates that the character was influenced by the action of additive genes [10] which also show that the character was influenced by genetic factors, so that selection could be done through a diversity of phenotypes [25].
The estimated value of high heritability with low values of genetic advance was found in the days to flowering, days to harvest, and panicle length (Table 4). This indicates that those characters were influenced by the action of non-additive genes [10]. It could also be interpreted that the days to flowering, days to harvest, and panicle length in the F2 lines have reached fixation of a stable age-regulating gene. The results of this study was the same as those of Akinwale et al. [25] against 20 Nigerian rice genotypes with heritability and genetic advance in days to flowering of 95.1% and 8.97 as well as 92.1% and 6.63 in days to harvest. Seyoum et al. [26] reported a heritability value of 90.16% and a genetic advance of 9.65 in the days to flowering, as well as 82.45% and 4.97 for the value of heritability and genetic advance for the days to harvest. Kristamtini et al. [3] also reported a high heritability value of 90.55% and a genetic advance of 1.87 in the days to flowering, as well as 90.72% and 1.87 in the days to harvest.
CONCLUSION
The black rice lines G1, G2, G8, and G20 were high in yields potential (7-8 t/ha) with high panicles length, filled grain number, weight of 100 grains, and grain yield per clump. Days to harvest, plant height, number of productive tillers, panicle length, and number of filled grains were characters that showed a high heritability. The number of productive tillers, number of filled grains, number of un-filled grains, and grain yield per clump were characters that were high in genetic diversity estimates, and the number of filled grains showed a high genetic advance.
ACKNOWLEDGEM ENTS
The authors would like to thank to the Directorate of Research and Community Service of Directorate General of Research and Development of the Indonesian Ministry of Research, Technology and Higher Education for the "PTUPT" research grand to run this research project through Decentralization Research Scheme of 2018-2020.
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