Peculiarities of generation of silver nanoparticles created for giving antibacterial properties to polymers in different fluids
D.E. Fominov1*, Yu.Yu. Karlagina1, Y.S. Andreev1, G.V. Romanova1
1-ITMO University, Russian Federation, 197101, St. Petersburg, Kronverkskypr. 49, lit. A.
* denis_fominov@niuitmo.ru
Surgical sutures are the main method of joining tissues after surgery or wounds. However, this method is prone to inflammation due to bacterial contamination. The most common way to prevent such infections is to modify the suture material with antibiotic drugs. However, given the increasing number of antibiotic-resistant bacteria [1,2], as well as undesirable side effects such as toxic effects, the need for repeated use [3], and allergic reactions, there is a need to search for alternative suture materials. One of the promising approaches is the use of suture material with antibacterial properties by enriching it with nanosilver. Our concept is to create a resorbable suture material with a bactericidal effect using colloidal silver obtained by laser ablation in liquid, the antibacterial activity of which has been proved [4].
The realization of this idea includes three main steps: 1. Development of a method for generating nanoparticles in liquid; 2. Investigation of bactericidal properties of the material used for suture material; 3. Development of the technology of creating resorbable suture material. The purpose of this work is to study the result of the process of obtaining colloidal silver in distilled water and physiological solution by laser ablation. In order to achieve this goal, the following tasks should be solved: 1. To synthesize silver nanoparticles solution by laser ablation method in distilled water; 2. To synthesize silver nanoparticles solution by laser ablation method in physiological solution; 3. Analyze the properties of the obtained colloids depending on the parameters of the laser beam and ablation medium; 4. To carry out comparative analysis.
The experiments were made using the Minimarker-2 laser complex equipped with an ytterbium fiber laser with a wavelength of 1064 nm. Silver plates (99.9% purity, sample 999) were used as a material. Before ablation, the material was mechanically polished using a mini-drill and polishing pastes, and cleaned in an ultrasonic bath in deionized water. Distilled water and 0.9% aqueous NaCl solution were used as ablation media. Characterization of the obtained nanoparticle solutions was carried out by dynamic light scattering and scanning electron microscopy methods with preliminary application of colloid on silicon plates.
Experiments were carried out to optimize the parameters of laser radiation for the generation of silver nanoparticles by pulsed laser ablation in liquid. The influence of laser beam parameters (power, pulse duration) and ablation medium (distilled water and physiological solution) on colloid characteristics was investigated. According to the results of colloid analysis, it was found that the used mode provides generation of silver nanoparticles with the size of 50-250 nm. The present study is an important part that determines the possibility of further research within the purpose of the whole project.
This research was supported by Priority 2030 Federal Academic Leadership Program.
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