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ALT'23 The 30th International Conference on Advanced Laser Technologies
B-I-7
Optical evaluation of differences in the diffusion of probe molecules in normal and pathological human kidney tissues
M. Pinheiro1, I. Carneiro23, S. Carvalho4, R. Henrique2'5, V. Tuchin6'7, L. Oliveira18
1- Institute for Systems and Computer Engineering, Technology and Science (INESC TEC), Campus da FEUP,
Rua Dr. Roberto Frias, 4200-465 Porto, Portugal
2- Department of Pathology and Cancer Biology and Epigenetics Group, Portuguese Oncology Institute of
Porto, Rua Dr. Antonio Bernardino de Almeida, 4200-072 Porto, Portugal
3- Department of Pathological, Cytological and Thanatological Anatomy, Polytechnic of Porto - School of
Health (ESS), Rua Dr. Antonio Bernardino de Almeida, 4200-072 Porto, Portugal
4- Department of Pathology, Santa Luzia Hospital (ULSAM), Estrada de Santa Luzia, 4904-858 Viana do
Castelo, Portugal
5- Department of Pathology and Molecular Immunology, Porto University - Institute of Biomedical Sciences
Abel Salazar, Rua de Jorge Viterbo Ferreira, n° 228, 4050-313 Porto, Portugal
6- Institute of Physics and Science Medical Center, Saratov State University, 83 Astrakhanskaya str., Saratov
410012, Russia
7- Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the FRC "Saratov Scientific Centre of the Russian Academy of Sciences, " 24 Rabochaya, Saratov
410028, Russia
8- Physics Department, School of Engineering, Polytechnic of Porto, Rua Dr. Antonio Bernardino de Almeida n°
431, 4249-015 Porto, Portugal
Main author email address: [email protected]
The application of kinetic spectroscopy and thickness measurements has been widely used to evaluate the diffusion properties of optical clearing agents (probe molecules) in tissues under treatment with solutions of different osmolarity [1]. Such methodology can be useful in various fields, such as dermatology, cosmetics or cryopreservation of organs and food products [2]. By performing those measurements from human colorectal mucosa tissues, in addition to discriminated diffusion properties in normal and pathological (adenocarcinoma) mucosa, it was possible to evaluate a higher mobile water content in the diseased tissue [3]. Since both the discriminated diffusion properties and different mobile water content in normal and diseased tissues can be used in diagnostic procedures, human kidney tissues, both normal and with chromophobe renal cell carcinoma (CRCC) were submitted to those measurements when under treatment with glycerol or sucrose solutions. It was found in this study that the characteristic diffusion coefficient of glycerol was 4.85x10-7 cm2/s in the normal kidney and 3.16x10-7 cm2/s in the CRCC kidney, while the characteristic diffusion coefficient of sucrose was 4.63 x 10-7 cm2/s in the normal kidney and 3.31 x 10-7 in the CRCC kidney. Apart from the discriminated diffusion properties of glycerol and sucrose in both tissues, it was also found that the CRCC kidney contains about 5% more mobile water than the normal kidney. Now that such difference in the mobile water content was found for two different cancers, it will be interesting to evaluate it as a function of cancer progression.
[1] I. Carneiro, S. Carvalho, R. Henrique, L. Oliveira and V. Tuchin, A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues, J. Biophotonics, vol. 12, pp. e201800333 (2019).
[2] L. Oliveira and V. Tuchin, The Optical Clearing Method: A New Tool for Clinical Practice and Biomedical Engineering (Springer), Chapter 6, (2019).
[3] S. Carvalho, N. Gueiral, E. Nogueira, R. Henrique, L. Oliveira and V. Tuchin, Glucose diffusion in colorectal mucosa - a comparative study
between normal and cancer tissues, J. Biomed. Opt., vol. 22, pp. 091506 (2017).
