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Optical Coherence Tomography as a Tool
in Reproductive Gynecology
DOI 10.17691/stm2015.7.1.12
Received December 10, 2014
O.G. Panteleeva, MD, PhD, Obstetrician-Gynecologist1;
I.A. Kuznetsova, MD, D.Med.Sc., Obstetrician-Gynecologist2;
O.V. Kachalina, MD, PhD, Associate Professor, Department of Obstetrics and Gynecology,
Postgraduate Faculty3;
D.D. Eliseeva, Assistant, Department of Obstetrics and Gynecology3;
Е.V. Grebenkina, MD, PhD, Oncologist, Photodynamic Therapy Department4;
S.V. Gamayunov, MD, PhD, Head of Photodynamic Therapy Department4; Assistant Professor,
Department of Oncology, Postgraduate Faculty3; Senior Research Fellow5;
S.S. Kuznetsov, MD, D.Med.Sc., Professor, Department of Pathological Anatomy3; Leading Research Fellow5;
E.E. Yunusova, MD, PhD, Associate Professor, Department of Pathological Anatomy3;
E.V. Gubarkova, PhD student, Junior Research Fellow, Research Institute of Biomedical Technologies3;
M.Yu. Kirillin, PhD, Senior Research Fellow6; Senior Research Fellow, Research Institute of Biomedical Technologies3;
Associate Professor5;
N.М. Shakhova, MD, D.Med.Sc., Leading Research Fellow, Department of Radiophysical Methods in Medicine6;
Leading Research Fellow, Research Institute of Biomedical Technologies3; Leading Research Fellow,
Femtosecond Biophotonics Laboratory5
1Railway Clinical Hospital on Gorky station, Joint Stock Company “Russian Railways”, 18 Prospekt Lenina,
Nizhny Novgorod, 603140, Russian Federation;
2N.A. Semashko Nizhny Novgorod Regional Clinical Hospital, 190 Rodionova St., Nizhny Novgorod, 603126,
Russian Federation;
3Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005,
Russian Federation;
4Nizhny Novgorod Regional Oncologic Hospital, 190 Rodionova St., Bldg. 5, Nizhny Novgorod, 603126,
Russian Federation;
5Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, Nizhny Novgorod, 603950,
Russian Federation;
6Institute of Applied Physics, Russian Academy of Sciences, 46 Ulianova St., Nizhny Novgorod, 603950,
Russian Federation
The objective of the investigation was to show the advisability of using optical coherence tomography (OCT) for noninvasive diagnostics
and monitoring of treatments in gynecological practice for female patients of reproductive age.
Materials and Methods. OCT studies were performed during standard gynecological procedures like laparoscopy and colposcopy, as well as
in course of pelvic inflammatory diseases of cervical neoplasia, with the use of an OKT1300U (BioMedTech, Russia). 330 patients of reproductive
age (aged 18–45) were examined. The methods used for numeric processing of the OCT images were based on an analysis of histograms and on
calculation of the first derivative of the OCT signal over depth.
Results. The use of OCT with numeric processing of the images increases the sensitivity of laparoscopy diagnosis of latent chronic
salpingitis from 43.5 to 96.0%. In the case of minimal endometriosis, OCT can be used as a substitute for excisional biopsy for the visualization
and verification of endometrioid heterotopias. The use of OCT in the case of cervical neoplasia in female patients of reproductive age enables a
considerable decrease in surgical aggression during the diagnosis and treatment of cervical intraepithelial neoplasia and early forms of cancer.
Conclusion. The use of OCT helps to maximize individualization and organ preservation during the diagnosis and treatment of gynecological
pathology, and therefore to preserve the reproductive potential of young female patients.
Key words: optical coherence tomography; laparoscopy; colposcopy; pelvic inflammatory diseases; endometriosis; neoplasia of the cervix;
photodynamic therapy.
The reproductive function is one of the most important functions of the woman. In recent decades, issues of the preservation and restoration of reproductive health have become particularly topical. This is connected with the
increase in the number of infertile couples, as well as with an increasing incidence of diseases which threaten reproductive potential.
The percentage of infertile couples in many countries
corresponding author: Gubarkova Ekaterina Vladimirovna, e-mail: [email protected]
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constitutes about 15% and shows no sign of decreasing while 28% of these cases represent so-called unexplained infertility [1]. This high percentage of unexplained infertility is, to a great extent, connected with imperfections in the methods of diagnosis used, which are incapable of detecting the insignificant changes and latent processes which are prevalent among the reasons for reproductive dysfunction [2, 3]. As a rule, the final stage in the diagnosis of female infertility is laparoscopy. However, the relevance of this method and its possibilities are currently being disputed. Thus, as has been found, in identifying one of the most frequent causes of reproductive dysfunction, pelvic inflammatory diseases, laparoscopy demonstrates a very low sensitivity, of 27% with a high specificity of 92%, resulting in a diagnostic accuracy of 78% [4]. Nevertheless, laparoscopy remains the gold standard for clarifying the etiology of female infertility [5, 6], thus making enhancement of the procedure through the use of supplementary methods appropriate and relevant. Optical coherence tomography (OCT) is one such method and the possibilities for its complementary use with laparoscopy are currently being studied [7, 8].
A further threat to the reproductive potential of women is gynecological disease, the treatment of which may be associated with loss of reproductive function. Among the most notable diseases of this type are tumors of the female pelvic organs including malignant neoplasms. The fact that malignant tumors are getting “younger” necessitates the development of organ preservation treatment but is possible only at the early stages of the disease [9]. In its turn this determines the requirement for more effective methods of diagnosis and monitoring of the treatment: maximal information and minimal invasiveness. OCT, being a noninvasive method for obtaining information about the structure of biological tissues at the level of tissue layers, has established its value in the diagnosis of early forms of cervical neoplasia, the “youngest” oncological disease among women [10, 11]. Detection of cervical neoplasia in reproductive age women at the stage of cervical intraepithelial neoplasia (CIN) and/or cancer in situ, allows for photodynamic therapy (PDT) which is currently the least aggressive method of treatment of this pathology, enabling preservation of the reproductive function [12]. Along with its advantages, PDT has one serious disadvantage connected with impossibility of morphological control of the treatment. This determines the need for the development of effective noninvasive methods for monitoring PDT, undoubtedly including OCT, the potential of which for the evaluation of minimally invasive procedures has been demonstrated [13].
The objective of the investigation is to demonstrate the feasibility of OCT for noninvasive diagnostics and the treatment monitoring in gynecological practice among patients of reproductive age. The article presents an overview of our previous results.
Materials and Methods. In this study OCT inspection was performed to complement standard gynecological procedures like laparoscopy and colposcopy, as well as at different stages of PDT of the cervix. The instrument used was an OKT1300U (BioMedTech, Russia) with the
following technical parameters: central probing wavelength of 1280 nm, in-depth resolution of 15 pm, lateral resolution of 20 pm, image acquisition rate of 8 frames per second, probing depth up to 1.4 mm. The instrument is equipped with a removable endoscopic probe 2.4 mm in diameter. The video mode allows to obtain information not only about the morphological, but also on the functional characteristics of the biotissues.
The inclusion criterion was the reproductive age of the patients, from 18 to 45. In total 330 female patients were examined with the use of OCT, including 180 patients with pathology of the pelvic organs (OCT laparoscopy) and 150 patients with early neoplasia of the cervix (OCT colposcopy), 30 of these were examined during PDT. Interpretation of the OCT images was performed in accordance with the criteria developed on the basis of morphologically verified diagnoses; the morphology data are represented in [14-15].
Methods of numeric processing of OCT images were used for objectification of the data. These were based on the analysis of histograms and the calculation of the first derivative of the OCT signal over in-depth direction [8]. The first method enables automatic differentiation of those cases which are characterized by different specific levels of OCT signal (e.g. normal tissues are characterized by a lower level of OCT signal compared to those with fibrosis), whereas the second method enables cases to be identified where contrasting areas are present in the OCT images (which is typical of edemas).
Results and Discussions
OCT in the diagnosis of infertility. We used OCT as a supplementary method during standard laparoscopy in patients with preliminary diagnoses of “unexplained infertility” with minimal clinical manifestations and nonobvious anamnesis of the disease. It was relevant that the data from the laparoscopy were also uninformative: in most cases no apparent anomalies being detected or evidence of a chronic inflammatory process or endometriosis was indirect. To obtain the OCT images an OCT probe was inserted through an extra trocar into the pelvic cavity and fixed by minimal pressing onto the serosa of the isthmic part of the fallopian tube or onto the parietal peritoneum for several seconds. The images obtained were interpreted in accordance with the previously developed criteria [15, 16].
During the study, examinations of fallopian tubes revealed three completely different types of OCT image (Figure 1). A structureless image with moderate signal intensity testified to a lack of any fallopian tube pathology (Figure 1 (a)). Heterogeneity in the structure of the OCT image testified to morphological heterogeneity of the muscle layer of the fallopian tube which evidences the presence of chronic salpingitis (Figure 1 (b), (c)). It is clinically significant that the OCT images allowed us, not only to diagnose chronic salpingitis, but also to identify the pathomorphological form of the inflammation. A dominance of inclusions with a low signal level (Figure 1 (b)) testifies to a predominantly exudative pathomorphological variant, whereas the presence in the image of “bright” areas where there was a greater signal intensity testified to fibrosis processes. Frequently, OCT images with a variety of heterogeneities were encountered.
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90 STM J 2015 — vol. 7, No.1 O.G. Panteleeva, I.A. Kuznetsova, O.V. Kachalina, D.D. Eliseeva, E.V. Grebenkina.N.M. Shakhova
PRECLINICAL AND CLINICAL ANALYSIS
Figure 1. OCT images: (a) unaltered fallopian tube, with moderate intensity of the signal, a structureless image, and decreased intensity with depth of the image; (b) chronic salpingitis, morphologically — the prevalence of edema, the heterogeneity of the image with zones of irregular form having low signal levels; (c) chronic salpingitis, morphologically — the prevalence of fibrosing, and local increases in signal intensity
1 mm
The data obtained are of great clinical importance. First of all, OCT effectively increases the sensitivity of laparoscopy in regard to the recognition of chronic pelvic inflammatory diseases. We carried out an open test to identify the diagnostic accuracy of laparoscopy and OCT laparoscopy, during which the symptoms of inflammation, based on laparoscopy, and on the OCT images, were identified among 29 patients with known, morphologically proved, diagnosis of chronic salpingitis. It was found that the sensitivity of laparoscopy alone was 43.5%, whereas the complementary use of OCT increased that percentage up to 95.7%. A more accurate assessment in the diagnosis of inflammatory changes to the fallopian tubes was provided by a “blind” test where a similar result was obtained; here the sensitivity of OCT laparoscopy was 90%. A fairly good kappa index of agreement amongst specialists (0.63; confidence interval 95% [0.5082; 0.7453]) suggests the sufficient objectivity of our proposed approach [17].
Another aspect of the clinical importance of our findings relates to the possibility of the noninvasive diagnosis of pathomorphological variants of the disease. Until now, such information has been unavailable in vivo, so there has previously been no division into variants. In planning their
treatment all patients underwent comprehensive therapy in accordance with the existing standards. By obtaining information regarding the prevalence of sclerosis of the muscular wall of the fallopian tube in vivo, we believe it is advisable to abandon conservative treatment in favor of assisted reproductive technologies to reduce the pill burden, the term and the cost of treatment.
Another cause of infertility which is difficult to diagnose is the so-called minimal endometriosis. The only method of identifying this type of endometriosis is still laparoscopy, with the method of verification being histological examination of bioptates. The small size and diversity of the manifestations of endometric heterotopias, as well as the peculiarities of their localization, make their visualization, interpretation and biopsy difficult, and sometimes impossible [18]. We used OCT for probing “endometriosis-suspicious” areas of the pelvic peritoneum which had been detected during standard laparoscopy. It was found that the OCT images of unchanged peritoneum indicated that it was formed from alternating layers of thin serosa, fatty tissue, and small vessels which made up the total “laminated” image (Figure 2 (а)). In study of endometriosis, objects of irregular shape with clear contours with alternating low and high
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В
X*r
* 4%
1 mm
Figure 2. OCT images of the pelvic peritoneum: (a) typical layered image of the area of the unaltered peritoneum; (b) spot (focus) of endometriosis represented by two cavities with heterogeneous content, with sharp borders which correspond to a morphological capsule; the increased intensity of the signal around the cavities corresponds to fibrosing of the tissues in contact with the endometriosis lesion
intensity signals could be visualized (Figure 2 (b)). The features of OCT images of endometriosis lesions are determined by their morphological peculiarities: the clear contours, are those bounded by the presence of a capsule, the heterogeneity of the signal — by their heterogeneous content, and the increase of the signal intensity around the inclusions — with collateral fibrosing due to the reactive perifocal inflammation. The size of the detected objects is
also of importance (1-2 mm) as well as their localization at arbitrary depth with regard to the peritoneum surface. These changes are hard to identify in standard laparoscopy.
The example provided shows that the capacity of OCT to visualize the inner structure of the tissue can be successfully used for the detection of minimal endometriosis. In the case of complicated localization of endometriosis heterotopias around the vessels, on the visceral peritoneum of the fallopian tube, or in the gate area of the ovary, biopsy is difficult. In these cases, OCT can substitute such an invasive procedure.
OCT in the diagnostics of cervical neoplasia. The wider use of noninvasive diagnostic technologies in the group of patients of reproductive age is reasonable, not only for diagnosis of infertility, but also for other gynecological pathology, such as cervical neoplasia. Firstly, cervical cancer is “the youngest” among all female tumors. Early revealing CIN and cancer in situ makes it possible to preserve fertility, but there is a problem of the effective diagnosis of the pathology of the cervix [19]. This is connected not only with the insufficient sensitivity of traditional methods of diagnosis but also with the low specificity of the gold standard — colposcopy. To compensate for this, the use of multi-focus biopsy or wide excisions of the cervix have been suggested. This diagnostic aggression is, to a certain degree, a risk to reproductive potential.
In this work we have suggested the use of OCT to reduce the number of unwarranted excision procedures. First and foremost, the OCT criteria which have been developed in relation to neoplasia of the cervix [10, 14] allow us confidently to abandon the need for biopsy after
Figure 3. Scheme for planning biopsy and the resection line in cervical neoplasia: points A, C, D are typical examples of a “malignant” OCT image with the signs of a lack of structure and low depth of the signal; points B and E are typical examples of a “benign” OCT image: a structured image with clear borders between the layers and an informative signal throughout the whole depth of the image
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92 STM J 2015 — vol. 7, No.1 O.G. Panteleeva, I.A. Kuznetsova, O.V. Kachalina, D.D. Eliseeva, E.V. Grebenkina.N.M. Shakhova
PRECLINICAL AND CLINICAL ANALYSIS
Figure 4. OCT image of CIN III: (a) before PDT, with typical signs of pre-cancer of the cervix — a lack of structure with a slight decrease in the signal depth; (b) immediately after PDT, and the appearance of a boundary between the epithelium and the stroma due to these layers contrasting with the interstitial fluid; (c) one day after PDT, with increased signs of tissue edema, expansion and congestion of the vessels; (d) 6 days after PDT, showing total necrosis of the tumor, a structureless image with a sharp decrease in the signal depth; (e) one month after PDT — repair of the exocervix, with signs of the typical image of cervix, moderate scattering by the upper layer corresponding to the stratified squamous epithelium, and the lower layer — with more effectively scattering by the stroma, blurred boundary between the layers showing the presence of weakly scattering linear inclusions in the lower layer, corresponding to the reactive inflammation
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obtaining a typical “benign” OCT image (points B and E in Figure 3) even if there are so-called atypical colposcopic signs. However, if there are OCT signs of malignancy (points А, C and D in Figure 3) biopsy is obligatory. This approach can reduce the number of false-positive conclusions from colposcopy, and in about 40% of cases allows us to refuse from biopsy [20]. Long-term observation of patients (for more than 3 years) have not revealed any progression of the pathology of the cervix in those cases of refusal from invasive diagnostics, and this retrospectively confirms the adequacy of the applied OCT criteria, and such tactics in general.
Optimization of the algorithm for the diagnosis of neoplasia of the cervix with the use of OCT is not only relevant to biopsy of the cervix. The presence of “malignant” signs in OCT images in different sectors of the cervix is a justified indication for diagnostic excision rather local spot biopsy. When planning the resection line it is also advisable to use OCT. In Figure 3 (point D) it can be seen that in an OCT investigation of the visual border of the pathological zone the image shows the transition of one type of structure into another, and only after retreating several millimeters it is possible to obtain a typical “benign” image. This information can be used during excision to ensure the adequate removal of the tumor. This approach allows us to provide maximal oncological effectiveness for the resection whilst retaining functional preservation of the organs.
In recent years photodynamic therapy has been implemented as a standard for treating patients of childbearing age with early neoplasia of the cervix, as an alternative to using surgical methods.
Along with a number of advantages, PDT has some limitations: the unpredictability of the photodynamic reactions, the influence of the degree of tumor oxygenation on the results, the lack of excision material for morphological monitoring of the treatment and the empirical character of the mode selection [21]. It is possible to minimize the influence of the weaknesses of PDT with the use of effective methods of monitoring (which are noninvasive and realized quickly in real-time) and here OCT undoubtedly has the required characteristics.
Figure 4 shows a sequence of OCT images of the cervix of female patient M., 32 years old, with a diagnosis of CIN
III. Before the treatment, the OCT criteria (structureless and with reduced signal depth) correspond to the diagnosis (Figure 4 (а)). Two hours after the injection of photosensitizer, and directly after the laser exposure the OCT image demonstrates signs of structural change occurred manifested by the increased contrast in the image indicating the typical pathomorphological reaction, tissue edema. (Figure 4 (b)). After one day (Figure 4 (c)) signs of hyperemia and blood stasis are increasing, while, after 6 days (Figure 4 (d)), signs of total tumor necrosis are present due to total failure of the blood circulation. After a month (Figure 4 (e)) no tumor was registered in the control biopsy, and there were the signs of repair associated with reactive inflammatory changes.
The example provided vividly indicates the possibility for noninvasive observation of the structural changes of the ectocervix in response to PDT; and it demonstrates the
prospects of OCT for the dynamic monitoring of complete and timely pathomorphological reactions.
It should be said that the mechanism of PDT is based on the interaction of several components (the photosensitizer, light and oxygen) with the implementation of several types of reaction [21]. Unfortunately, OCT can only retrospectively assess the results of PDT, but not the process of action in real time. This is why, for effective monitoring, it is useful to combine several methods of bioimaging. In our view its combination with fluorescent visualization is particularly effective, as it allows assessment of the accumulation and photobleaching of the photosensitizer [22]. Other authors have also suggested combinations of methods, for example, for the assessment of photosensitizer photobleaching and the dynamics of singlet oxygen [23]. Another approach is based on observation of the basic PDT mechanism with an assessment of microcirculation and tissue oxygenation [24]. In this regard a modification of the OCT approach with implementation of the visualization of the microvasculature is required [25].
However, despite we have shown the attractiveness of OCT in situations where its noninvasiveness is indispensable, there is a need for further enhancement and modification of the method. One such is automation of the interpretation of the OCT images. It should be noted that a specialist's subjective assessment of a diagnostic image can sometimes differ from the actual situation due either to the changes in the OCT image not being readily visible or to insufficient training of the specialist. In these cases it would be effective to apply numeric processing to the OCT images as this could numerically characterize the image to help avoid subjectivity in the assessment. For differential diagnostics it is logical to use several numeric criteria that reveal different changes in the object and which therefore allow not only identification of the presence of particular pathologies, but could also determine their extent. In the OCT investigations of fallopian tubes we used an approach involving the calculation of the two values from OCT images which characterize the degree of edema and tissue fibrosing. The use of these values in the assessment of diagnostic OCT images should enable increased sensitivity of the method up to 96% compared to the 90% already demonstrated in “blind” testing without numeric processing [8, 17].
Conclusion. Our study showed that employment of OCT as a supplementary tool during standard procedures increases the effectiveness of the diagnosis of those gynecological pathological states which are difficult to recognize. The use of OCT for patients of reproductive age is justified to a great extent as, in this case, the enhanced information is not accompanied by any additional invasiveness.
As a supplementary method for recognition of the causes of infertility OCT has shown a high sensitivity to latent forms of pelvic inflammatory diseases. The possibility of visualizing and verifying minimal endometriosis with OCT is of great interest.
The complementary use of OCT during colposcopy allows a reduction in the aggressiveness of the diagnosis of pre-cancer and early forms of cervical cancer. OCT
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94 STM J 2015 — vol. 7, No.1 O.G. Panteleeva, I.A. Kuznetsova, O.V. Kachalina, D.D. Eliseeva, E.V. Grebenkina.N.M. Shakhova
PRECLINICAL AND CLINICAL ANALYSIS
monitoring of photodynamic therapy contributes to the optimization of this organ-preserving method of treatment of early forms of cervical neoplasia.
One further advantage is provided by the numeric processing of OCT images as it will allow us to avoid the subjectivity of specialists during the assessment of images and will therefore increase the diagnostic accuracy of the method.
And finally the use of OCT can contribute to the maximal individualization and organ preservation during the diagnosis and treatment of gynecological pathology, and therefore to the preservation of the reproductive potential of female patients of reproductive age.
Study Funding. This article is a review of our studies which were, in different years, financed by the Program of the Presidium of the Russian Academy of Sciences “Fundamental Sciences to Medicine”, partially by grants from the Russian Foundation for Basic Research and those linked to the projects of the Ministry of Education and Sciences of the Russian Federation, including the studies currently being performed on PDT monitoring, where the clinical trials are supported by Project No.14.В25.31.0015, technological studies on Project RFMEFI60414X0027.
Conflicts of Interest. The authors have no conflicts of interest.
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