CC BY
22B-PS-4
Optical clearing of human gingival mucosa: in vitro studies
A.A. Selifonov12, V.V. Tuchin1345
1Saratov State University, Chair of Optics and Biophotonics, Saratov, Russian Federation 2Saratov State Medical University, Department of Dentistry, Saratov, Russian Federation 3ITMO University, Chair of Optics and Biophotonics, St. Petersburg, Russian Federation 4Institute of Precision Mechanics and Control of the Russian Academy of Sciences, Chair of Optics and Biophotonics, Saratov, Russian Federation
5Tomsk State University, Chair of Optics and Biophotonics, Tomsk, Russian Federation
Due to the significant development of various optical technologies and methods used both for noninvasive diagnostics of biological tissues and for optimized protocols of photodynamic therapy, photothermal destruction, optical biopsy, tomography, and etc., the problem of increasing the depth of light penetration in biological tissue is relevant. An opportunity to change optical parameters of the top layers of tissues reversibly (reduction of the scattering properties), in particular in stomatology, can expand methods of diagnostics and therapy [1]. Very often hyperosmotic agents, such as glucose, sorbitol, glycerol, polyethylene glycol, propylene glycol, dimethyl sulphoxide, and isosmotic solutions, such as X-ray contrast substance iohexol, etc. are used as optical clearing agents (OCAs) [2,3]. In this work, the effective diffusion coefficient of 40%-glucose-aqueous solutions in human gum mucous tissue was determined in vitro. The method is based on the registration of the kinetics of changes in the spectra of diffuse reflection and the application of the free diffusion model. The effectiveness of the optical clearing of the human gum mucous tissue in the stage of completion of the process of diffusion of 40%-glucose-aqueous solutions was also determined. Due to the use of hyperosmotic agents (for example, glucose), under conditions of applicability of the method of optical clearing (OC), it is possible to increase the penetration depth of laser radiation into biological tissue, which is important for the diagnosis and therapy of oral diseases in the early stages.
The work was supported by grant of the Russian Federation Governmental grant 17-00-00272 (17-00-00275 (K)).
References
[1] D. K. Tuchina, P. A. Timoshina, V. V. Tuchin, A. N. Bashkatov, and E. A. Genina. IEEE J. Select. Tops Quant. Electron. 2019. 25 (1), 7200508.
[2] Carneiro I., Carvalho S., Henrique R., Oliveira L. M., Tuchin V. V. A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues, J. Biophotonics. 2019, 12:e201800333.
