CC BY
30Russian Journal of Nematology, 2013, 21 (1), 31 - 40
On the identity of Labronema vulvapapillatum (Meyl, 1954) Loof & Grootaert, 1981 (Dorylaimida, Qudsianematidae)
12 1
Ebrahim Shokoohi , Joaquín Abolafia , Abdolrahman Mehrabi-Nasab
2
and Reyes Peña-Santiago
1Departament of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran; e-mail: eshokoohi@mail.uk.ac.ir 2Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén. Campus "Las
Lagunillas" s/n. 23071-Jaén, Spain
Accepted for publication 7 May 2013
Summary. Iranian populations of Labronema vulvapapillatum are studied in depth, including morphological and molecular data. They are characterised by having 1.76-2.50 mm long body in females and 1.55-2.19 mm in males, lip region offset by marked depression and 19-22 ^m wide in females and 2024 ^m in males, odontostyle 22-26 ^m long in females and 21-31 ^m in males or 1.0-1.3 times the lip region width, neck 379-495 ^m long in females and 366-484 ^m in males, pharyngeal expansion 175-235 ^m long in females and 164-226 ^m in males or occupying 43-49% of total neck length, female genital system didelphic, uterus tripartite with a short intermediate section becoming a Z-organ and 199-254 ^m long or 2.6-4.0 times the body diameter, pars refringens vaginae present, vulva longitudinal (V = 49-59), tail short and rounded, (17-37 ^m, c = 72-114, c' = 0.5-0.9 in females; 19-28 ^m, c = 59-82, c' = 0.6-0.8 in males), spicules 55-76 ^m long and 16-25 contiguous ventromedian supplements. Iranian material is compared with other known populations, concluding that there are no relevant morphological differences among them but wide overlap in their main morphometrics. The 18S rRNA gene sequence of Iranian L. vulvapapillatum is very close to other Labronema sequences, especially to the Dutch population of the same species.
Key words: Description, Iran, Labronema, morphology, phylogeny, SEM, SSU-rDNA, taxonomy.
Meyl (1954) identified one Italian dorylaimid female as "Dorylaimus obtusicaudatus Bastian, 1865 var. vulvapapillatus n. var.", provided a very short description and its Demanian ratios, and illustrated only the vulva region (original Fig. 39), in which one pre- and another post-vulval papillae were present. Andrassy (1959) classified this taxon under Eudorylaimus Andrassy, 1959 as E. vulvapapillatus (Meyl, 1954), but did not provide any further information about it. A few years later, however, the same author (Andrassy, 1962) studied and illustrated a couple of specimens of E. vulvapapillatus from Hungary. Loof and Grootaert (1981) re-described this species based on material collected in Scotland and maintained in culture in Belgium and Western Germany, and transferred it to Labronema Thorne, 1939. Subsequently, L. vulvapapillatum has been reported from The Netherlands (Bongers, 1988), Spain (Jimenez-Guirado, 1989; Murillo-Navarro & Jimenez-
Guirado, 2006), Korea (Choi et al., 1997) and Hungary (Andrassy, 2002). Some authors (Andrassy, 2002; Murillo-Navarro & Jimenez-Guirado, 2006) noted significant morphometric differences between known populations of L. vulvapapillatum and suggested they might belong to more than one species.
Iranian populations of this species were collected during an extensive nematological survey. Their study revealed new relevant data on the identity of L. vulvapapillatum. The results obtained are presented here.
MATERIALS AND METHODS
Nematode materials. Nematodes were collected from natural and disturbed habitats in Iran, killed by heat, fixed in 4% formaldehyde, processed to anhydrous glycerol according to De Grisse (1969) and mounted on glass slides. Morphometrics included
Table 1. Nematode species and GenBank accession number used for phylogenetic study.
Origin Reference GenBank accesion number Species
The Netherlands Holterman et al., 2006 AY284801 Allodorylaimus andrassyi
The Netherlands Holterman et al., 2006 AY284812 Aporcelaimellus cf. paraobtusicaudatus
UK Blaxter & Eyualem-Abebe, unpubl. DQ141212 Aporcelaimellus obtusicaudatus
The Netherlands Holterman et al., 2006 AY284828 Discolaimus major
The Netherlands Holterman et al., 2006 AY284821 Dorylaimellus montenegricus
The Netherlands Holterman et al., 2006 AY284776 Dorylaimus stagnalis
The Netherlands Holterman et al., 2006 AY284783 Ecumenicus monohystera
Iran Pedram et al, 2009 FJ042953 Enchodelus macrodorus
The Netherlands Holterman et al., 2006 AY284803 Epidorylaimus lugdunensis
Antarctica Raymond & Wharton, unpubl. HQ270136 Eudorylaimus sp.
United States Mullin et al, 2005 AY552972 Labronema ferox
The Netherlands Holterman et al., 2006 AY284807 Labronema vulvapapillatum
Iran Present paper KC574385 Labronema vulvapapillatum
The Netherlands Holterman et al., 2006 AY284831 Leptonchus granulosus
The Netherlands Holterman et al., 2006 AY284780 Mesodorylaimus sp.
The Netherlands Holterman et al., 2006 AY284806 Microdorylaimus modestus
The Netherlands Holterman et al., 2006 AY284826 Paractinolaimus macrolaimus
Belgium Meldal et al, 2007 AY993978 Paractinolaimus macrolaimus
The Netherlands Holterman et al., 2006 AY284788 Pungentus silvestris
The Netherlands Holterman et al., 2006 AY284785 Opisthodorylaimus sylphoides
The Netherlands Holterman et al., 2006 AY284786 Opisthodorylaimus sylphoides
The Netherlands Holterman et al., 2008 AY284824 Oxydirus oxycephalus
The Netherlands Holterman et al., 2006 AY284835 Tylencholaimus mirabilis
The Netherlands Holterman et al., 2006 AY284795 Thonus circulifer
The Netherlands Holterman et al., 2006 AY284794 Thonus minutus
India Oliveira et al., 2004 AY297821 Mononchus aquaticus (out group)
de Man's indices and most of the usual measurements. Some of the best preserved specimens were photographed with a Nikon Eclipse 80i microscope and a Nikon DS digital camera. Raw photographs were edited using Adobe® Photoshop® CS. After their examination and identification, a few specimens preserved in glycerin were re-processed for observation under SEM following the protocol by Abolafia and Peña-Santiago (2005). The nematodes were hydrated in distilled water, dehydrated in a graded ethanol and acetone series, critical point dried, coated with gold, and observed with a JEOL JSM-5800 microscope.
Phylogenetic analysis. The sequences of several species of the superfamily Dorylaimoidea used for phylogenetic analysis were obtained from the GenBank (Table 1). DNA extraction was done using an AccuPrep Genomic DNA extraction kit (Bioneer Corporation, Korea, http://www.bioneer.com) according to the manufacturer's instructions. Individual specimens (ten specimens belong to Khabr population) were picked
into 1.5 ml tubes containing 5 ^l double distilled water. Each tube was frozen in liquid nitrogen and was crushed using a vortex. Tissue Lysis buffer (TL; 200 pl) and 20 ^l Proteinase K (20 mg ml1) were added. The homogenate was incubated at 60°C for 2 h. The supernatant was extracted and stored at -20°C. The forward primer SSU_F_04 (5'-GCT TGT CTC AAA GAT TAA GCC-3') and the reverse primer SSU_R_26 (5'-CAT TCT TGG CAA ATG CTT TCG-3') (Blaxter et al., 1998) were used in the PCR reactions for amplification of the partial 18S region (~900bp). PCR was conducted with 10 ^l of the extracted DNA, 4 ^l of PCR Master Mix (Kawsar Biotech company, Iran), of each primers (10 pmol pl-1) and ddH2Ü to a final volume of 25 pi. The amplification was carried out using an Eppendorf Mastercycler gradient (Eppendorf, Hamburg, Germany), with the following parameters: 3 min at 94°C, 37 cycles of 45 s at 94°C, 45 s at 56°C and 1 min at 72°C, and finally one cycle of 6 min at 72°C
Fig. 1. Labronema vulvapapillatum (Meyl, 1954) Loof & Grootaert, 1981 (LM). A: Female, entire. B, C: Anterior region in median view. D: Female, posterior body region. E: Male, posterior body region. F: Lip region in lateral, surface view. G: Uterus, showing its three sections. H, K: Details of intermediate section (Z-organ) of uterus. I: Male, entire. J: Spicules. L: Vagina. (Scale bars: A, I = 500 ^m; B, H, K, J, L = 20 ^m; C, F = 10 ^m; D, E, G = 50 ^m).
Table 2. Morphometric data of Labronema vulvapapillatum (Meyl, 1954) Loof & Grootaert, 1981. Measurements in ^m (except L, in mm) and in the form: mean ± standard deviation (range).
Habitat Turf Turf Turf
Population Kerman Kerman
Khabr University Campus Baghe Melli
Character
n 522 733 222 233 322 333
L 2.12±0.26 1.83±0.19
(1.81-2.50) (1.55-2.09) 2.08, 1.94 1.71, 2.19 1.76-1.83 1.83-1.96
a 30.2±3.6 29.0±3.9
(26-36) (24-34) 33, 40 33, 35 25-30 22-31
b 5.0±0.1 4.4±0.3
(4.8-5.1) (3.8-4.7) 4.5, 4.7 4.5, 4.5 4.4-4.5 4.5-4.8
c 99.7±13.2 76.7± 8.6
(83-114) (65-77) 72, 92 73 ,59 77-90 74-82
0.67±0.2 0.7±0.1
(0.5-0.9) (0.6-0.8) 0.8, 0.6 0.7, 0.8 0.6-0.7 0.6-0.7
V 54.6±3.2
(52-59) _ 55, 51 _ 49-52 _
Lip region diameter 21.4±0.5 21.4±1.4
(21-22) (20-24) 21, 19 20, 22 19-21 20-21
Odontostyle length 26.8±1.6 26.9±2.2
(25-29) (24-31) 26, 20 21, 24 21-25 24-26
Odontophore length 44.8±3.6 43.1±3.9
(41-49) (39-49) 43, 41 37, 44 37-38 35-36
Neck length 424.0±44.4 417.6±35.9
463, 410 383, 484 386-411 388-427
(379-495) (366-470)
Pharyngeal expansion length 204.4±21.2 204.0±17.2
(175-235) (177-226) 198, 188 164, 224 178-200 186-195
Diameter at neck base 64.4±7.9 60.6±8.4
(56-76) (51-75) 58, 58 51, 59 59-67 55-74
mid-body 70.6±9.7 63.6±8.5
(59-86) (54-77) 64, 65 52, 63 65-73 63-82
anus 39.8±3.8 35.7±2.4
(34-43) (33-39) 37, 35 40, 39 41-44 36-39
Prerectum length 87.4±6.9 124±24
(75-91) (92-140) 67, 67 132, 132 71-109 165-189
Rectum/cloaca length 47.8±6.9 55.6±7.4
(40-56) (45-66) 48, 50 53, 60 41-44 67-72
Tail length 21.6±4.5 25.0±3.1
(17-28) (19-28) 62.0±6.5 37, 35 29, 30 20-23 24-26
Spicules length _ (55-73) _ 62, 73 _ 71-76
Ventromedian supplements _ 21-25 _ 21 _ 16-22
Fig. 2. Labronema vulvapapillatum (Meyl, 1954) Loof & Grootaert, 1981 (SEM). A: Anterior region in sublateral view: B, C: Lip region in face view. (Scale bars = 5 ^m).
followed by a holding temperature of 4°C. After DNA amplification, 5 ^l of product was loaded on a 1% agarose gel (40 mM Tris, 40 mM boric acid, and 1 mM EDTA) to verify amplification. The bands were stained with 50 mM ethidium bromide and visualised and photographed on 1% agarose gel under a UV transilluminator. Product was stored at -20°C prior to sequencing. The PCR product was purified for sequencing and sequenced with primers that were used for the amplification step. Sequencing was performed in both directions. The DNA sequence was edited using Chromas version 1.45 (McCarthy, 1997). Sequencing reactions were performed by the Bioneer Company (South Korea, http://eng.bioneer.com). Additional sequences for the ingroups and outgroups were obtained from NCBI GenBanks (Table 1).
The ribosomal SSU sequences were analysed and aligned using BioEdit (Hall, 1999). Phylogenetic trees were generated using the Bayesian inference method as implemented in the program Mr Bayes 3.1.2 (Ronquist & Huelsenbeck, 2003). The analysis under GTR model was initiated with a random starting tree and run with the Markov Chain Monte Carlo (MCMC) for 106 generations. Mononchus aquaticus (AY297821) was used as outgroup for this phylogenic analysis. This selection was based on a study by Alvarez-Ortega and Peña-Santiago (2012). The original partial 18S sequence of Labronema vulvapapillatum was deposited in GenBank under accession number KC574385. The Bayesian tree was visualised with the Tree View program (Page, 1996).
DESCRIPTION
Labronema vulvapapillatum (Meyl, 1954) Loof & Grootaert, 1981 (Figs 1 & 2)
Material examined. Ten females and 12 males from three localities, in good state of preservation.
Measurements. See Table 2.
Adult. Slender nematodes of medium size, 1.552.50 mm long. Habitus after fixation often curved ventrad, to an open 'C' in female, more curved ventrad in posterior body region in male, to G-shaped. Body cylindrical, tapering towards both ends, but more so towards the anterior extremity since the caudal region is rounded. Cuticle with very fine transverse striation, 3-5 ^m thick in anterior region, 3.5-5.0 ^m at mid-body and 3.0-8.0 ^m on tail. Lateral chord 15-20 ^m wide or occupying about one-fourth (19-29%) of mid-body diameter. Lateral pores small but visible throughout the body;
two to four dorsal and ventral, especially distinct, pores are present at level of odontostyle plus odontophore. Lip region weakly angular when observed in surface view but more rounded in median view, offset by marked depression (but a constriction is in no way perceptible), 2.8-3.8 times as broad as high, and about one-third (29-39%) of body diameter at neck base. SEM observations (Fig. 2): lip region hexagonal with straight sides and oral field perceptibly sunken (sucker-like) and bearing radial incisures; lips amalgamated, with a well demarcated perioral area, which is divided in six sectors (low liplets), the lateral ones trapezoidal, the others triangular; papillae located at the elevated margins of lip region and weakly protruding on surface of lips. Amphid fovea funnel-shaped, opening at level of the cephalic depression; its aperture 9-12 ^m wide or about one-half (43-55%) of lip region diameter. Odontostyle strong, 5.2-6.9 times as long as wide, hardly longer (1.0-1.3 times) than lip region diameter and 1.03-1.35% of total body length; aperture occupying about two-fifths (38-46%) of its length. Odontophore rod-like, 1.62.1 times as long as the odontostyle. Guiding ring double, with fixed ring at 14-17 ^m or about 0.6-0.8 times the lip region diameter from anterior end. Anterior slender region of the pharynx expanding very gradually; basal expansion 5.0-6.9 times as long as wide, 2.7-3.8 times as long as body diameter at neck base, and occupying up to one-half (4349%) of total neck length. Pharyngeal gland nuclei and their outlets as follows (n = 4, females): DN = 59-61, S1N1 = 71-73, S1N2 = 76-78, S2N = 88-89. Cardia rounded conoid, 15-25 * 11-19 ^m, surrounded by intestinal tissue that forms a long conical projection bulging into intestinal lumen. A dorsal cell mass is present at the anterior end of intestine in some specimens. Caudal region short and rounded.
Female. Genital system didelphic-amphidelphic, with both genital branches equally and very well developed, the anterior 344-410 ^m or 15-20% of body length, the posterior 329-407 ^m or 16-20% of body length. Ovaries reflexed, 155-258 ^m (anterior) and 61-233 ^m (posterior) long, very often reaching and surpassing the sphincter level; oocytes arranged in a single row except near its tip. Oviduct 106-141 ^m long or 1.4-2.1 times the body diameter, and consisting of a slender portion with prismatic cells joining the ovary subterminally and a well-developed pars dilatata, longer than wide, with distinct lumen and often containing sperm cells. A weak sphincter is present at oviduct-uterus junction. Uterus 194-254 ^m long or 2.6-4.0 times the body diameter; tripartite, i.e. consisting of an intermediate and short section representing a Z-organ (with thick
1.00
AY297821 Mononchus aquaticus
1.00
1.00
AY284826 Paractinolaimus macrolaimus AY993978 Paractinolaimus macrolaimus
0.50
0.50
— AY552972 Labronema ferox
— AY284807 Labronema vulvapapillatum
— KC574385 Labronema vulvapapillatum ^ P AY284783 Ecumenicus monohystera
AY284801 Allodorylaimus andrassyi
— AY284824 Oxydirus oxycephalus AY284777 Dorylaimus stagnaiis AY284780 Mesodoryiaimus sp.
- AY284785 Opisthodorylaimus sylphoides AY284786 Opisthodorylaimus sylphoides AY2848I2 Aporcelaimellus cf. paraobtusicaudatus
- DQ141212 Aporcelaimellus obtusicaudatus
1.0 0- AY284821 Dorylaimellus montenegricus
1.001 i- AY284828 Discolaimus major - AY284831 Leptonchus granulosus
0.50.
1.00
1.00
1.00
1.00
'00 AY284835 Tylencholaimus mirabilis AY284788 Pungentus silvestris HQ270136 Eudorylaimus sp. i— AY284795 Thonus circulifer — FJ042953 Enchodelus macrodorus - AY593946 Prodorylaimus mas
AY284803 Epidoiylaimus lugdunensis AY284778 Prodorylaimus uliginosus ■ AY284806 Microdorylaimus modestus
.00
•DO 1—
i.ooj_
1.00
L AY284794 Thonus minutus
0.1
Fig. 3. The Bayesian Inference tree of newly sequenced Labronema vulvapapillatum from Iran and closely related species belonging to the superfamily Dorylaimoidea based on 18S rDNA region from GenBank.
Table 3. Morphometric data of Labronema vulvapapillatum (Meyl, 1954) Loof & Grootaert, 1981. Measurements in ¡im (except L, in mm).
Population Italy Hungary Scotland/Germany Scotland/Belgium Spain Korea Hungary Spain Iran
Reference 1 2 3 3 4 5 6 7 g**
n 9 9 3 2199 nSS 899 sSS 1599 14,3,3 299 1199 16,3,3 1099 12,3,3
Character
L 1.55 1.96 1.73 1.88-2.62 2.06-2.45 2.68-3.00 2.57-3.24 2.46 1.54-2.27 1.41-1.98 1.42-1.45 1.37-1.72 1.44-1.79 1.76-2.50 1.55-2.19
a 25 25 27 28-36 31-35 26-32 27-37 23 26-37 26-35 25-26 22-27 23-29 25-40 22-35
b 4.0 4.4 4.3 4.1-5.3 4.4-5.0 4.9-5.4 4.8-5.6 4.9 4.3-5.1 4.0-5.0 3.9-4.0 3.8-4.4 3.9-4.6 4.4-5.1 3.8-4.8
c 67 72 60 68-104 67-81 79-105 73-93 85 79-157 68-108 52-62 60-87 59-70 72-114 59-82
c' ? 0.7* 0.8* 0.5-0.8 0.6-0.7 0.6-0.8 0.7-0.8 ? ? ? 0.6-0.7 0.5-0.7 0.6-0.7 0.5-0.9 0.6-0.8
V 54 53 - 51-50 - 50-55 - - 51-54 - 49-52 51-56 - 49-59 -
Lip region diameter ? 23* 22-23 22-23 ? ? ? 18-19 18-21 17-20 19-22 20-24
Odontostyle length ? 28 27-32 26-31 28-30 29-32 30 25-31 25-31 25 21-25 22-26 20-29 21-31
Odontophore length ? ? ? 38-44 38-43 41-45 41-47 ? ? ? ? 33-40 28-38 37-49 35-49
Neck length 388* 445* 402* 456-502 455-504 534-558 508-574 502 321-445 292-423 355-360 360-408 358-384 379-495 366-484
Pharyngeal expansion length ? ? ? ? ? ? ? ? ? ? 154-160 ? ? 175-235 164-226
Body diameter at mid-body 63* 78* 64* 61-79 62-78 91-110 76-119 107* 51-69 47-58 54-56 ? ? 56-76 51-75
anus/cloaca ? 39* 36* ? ? ? ? ? 29-38 31-37 ? ? ? 59-86 52-83
Prerectum length ? ? ? ? ? ? ? ? 48-99 ? ? 60-85 104-124 67-109 92-189
Rectum/cloaca length ? ? ? ? ? ? ? ? ? ? ? ? ? 40-56 45-72
Tail length 23* 27* 29* 23-29 26-35 28-35 34-38 29* 15-20 18-23 21-24 19-25 24-28 17-37 19-28
Spicules length - - 64 - 61-79 - 70-77 79 - 53-75 - - 58-66 - 55-76
Ventromedian supplements - - 20 - 19-24 - 19-24 21 - 19-21 - - 15-20 - 16-25
* Calculated from original measurements, ratios or illustrations.
** Including female and male specimens of three populations.
References: 1 - Meyl (1954, as Doiylaimus obtusicaudatus var. vulvapapiUatus). 2 - Andrássy (1962, as Eudorylaimus vulvapapiUatus). 3 - Loof and Grootaert ( 1981). 4 - Jiménez-Guirado (1989). 5 - Choi et. al. (1997). 6 - Andrássy (2002). 7 - Murillo-Navarro and Jimémez-Guirado (2006). 8 - Present paper.
►3 ►3
a
walls surrounded by strong circular musculature, narrow lumen, and containing irregular, often triangular or trapezoidal, refractive elements), and proximal and distal regions wider than the intermediate one, which become visibly dilated close the Z-organ, nearly spherical when they contain sperm cells. Vagina extending inwards 34-37 ^m or two-fifths to one-half (40-55%) of body diameter: pars proximalis 22-26 x 11-17 ^m, with almost straight walls, and circled by weak musculature, pars refringens with two small, 5-7 x 2-3 ^m, sclerotized pieces separated by a less sclerotized area and with a combined width of 13 ^m, and pars distalis 3-4 ^m long. Vulva an oval longitudinal slit. One female bearing one ventral papillae (paravulva) near the vulva. Prerectum 1.9-3.1 times, rectum 1.2-1.4 times the anal body diameter long. Caudal pores two pairs, one subdorsal and another sublateral, at the middle of tail. Male. Prerectum 3.0-5.0, cloaca 1.3-2.0 times the anal body diameter long. Genital system diorchic, with opposite testes. In addition to the ad-cloacal pair, located at 7-10 ^m from the cloacal aperture, there is a series of 16-24 contiguous ventromedian supplements, the most posterior of which situated at 70-86 ^m from the ad-cloacal pair, out the range of spicules. Spicules dorylaimoid, 4-0-5.6 times as long as wide (length measured along the curve), and 1.6-1.9 times the anal body width long. Lateral guiding piece 12-18 ^m long, 3.5-4.3 times as long as wide and furcate at the end. Caudal pores several pairs.
Molecular characterisation. One 18S rRNA gene sequence was obtained, consisting of 922 bps.
Diagnosis. Iranian populations of L. vulvapapillatum are distinguishable by having 1.762.50 mm long body in females and 1.55-2.19 mm in males, lip region offset by marked depression and 19-22 ^m wide in females and 20-24 ^m in males, odontostyle 22-26 ^m long in females and 21-31 ^m in males or 1.0-1.3 times the lip region width, neck 379-495 ^m long in females and 366-484 ^m in males, pharyngeal expansion 175-235 ^m long in females and 164-226 ^m in males or occupying 4349% of total neck length, female genital system didelphic, uterus tripartite with a short intermediate section becoming a Z-organ and 199-254 ^m long or 2.6-4.0 times the body diameter, pars refringens vaginae present, vulva longitudinal (V = 49-59), tail short and rounded, (17-37 ^m, c = 72-114, c' = 0.50.9 in females; 19-28 ^m, c = 59-82, c' = 0.6-0.8 in males), spicules 55-76 ^m long and 16-25 contiguous ventromedian supplements.
Distribution. Three localities in Kerman province: i) Baghe Melli (N: 30°16'48.97"; S: 057°04'01.27"); ii) Campus of the Shahid Bahonar
University of Kerman, (N: 30°15'27.10"; E: 57°06'13.59"); and iii) Khabr National Park (N: 28°81'71.54"; S: 056°32'66.04"), all populations in association with a seed mixture of Poa sp., Festuca sp. and Lolium sp.
Remarks. The Iranian material examined is morphologically identical, with no significant difference in its diagnostic features. Morphometrically, it also confirms a rather homogeneous group since only small differences in main measurements and ratios are observed between the representatives of the three populations, whose ranges, however, often show coincidence or wide overlapping. These interpopulation differences are certainly explainable on the basis of geographical variability and/or the low number of nematodes available in each case.
The characterisation and the taxonomy of L. vulvapapillatum have been a matter of discussion and deserve special attention. Table 3 compiles the main morphometrics of the populations hitherto studied. Available data show that L. vulvapapillatum displays remarkable variation in general size and, especially important, in odontostyle length. For instance, in relation to the size, body length always exceeds 2.5 mm in specimens from Scotland/Belgium (originally collected in Scotland but maintained in culture in Belgium, cf. Loof and Grootaert, 1981), whereas nematodes from other locations only exceptionally reach 2.5 mm and are under 2.0 mm in several cases. Concerning the odontostyle length, a more reliable feature than body length for characterising dorylaims, the range (20-32 ^m) is relatively large, with some overlapping when comparing specimens from distant locations, but also allowing their separation into two groups of populations whose respective ranges are 20-26 and 25-32 ^m. Several authors (Andrassy, 2002; Murillo-Navarro & Jimenez-Guirado, 2006) noted such differences and suggested that they might correspond to two different species. Nevertheless, leaving aside the odontostyle length, it is not possible to find other morphological or morphometric differences to define two separate patterns. On the contrary, there are several remarkable morphological features (among others, lip region offset by marked depression but never a distinct constriction, uterus tripartite with Z-organ, longitudinal vulva and presence of ad-vulval papillae) that confer a great homogeneity to the group.
A Blastn search of L. vulvapapillatum SSU region revealed the highest match with a Dutch
population of the same species (GenBank accession number AY284807), differing from this in 12 bps (identity 99%), as well as American population of L. ferox (accession number AY552972), from which it differs in 14 bps (98%). Figure 3 shows the evolutionary relationships of the material examined as derived from molecular analysis. The sequence of Iranian L. vulvapapillatum is included, together with other two Labronema sequences, in a large clade dominated by long-tailed dorylaimid taxa, confirming other recent result, e.g. those by Alvarez-Ortega and Peña-Santiago (2012) based on D2D3 region sequences.
Labronema vulvapapillatum is probably a widely distributed taxon in Northern Hemisphere, hitherto restricted to the Palearctic range, since it was never recorded out of Eurasia.
ACKNOWLEDGEMENT
The Spanish authors especially thank the financial support received from the project entitled Fauna Ibérica: Nematoda, Dorylaimoidea (excepto Longidoridae) (Spanish Ministry of Science and Innovation, ref. CGL2007-66786-C08-08; co-financed FEDER). SEM pictures were obtained with the assistance of technical staff and the equipments of 'Servicios Técnicos de Investigación', University of Jaén.
REFERENCES
Abolafia, J. & Peña-Santiago, R. 2005. Nematodes of the order Rhabditida from Andalucía Oriental: Pseudacrobeles elongatus (de Man, 1880) comb. n. Nematology 7: 917-926. Alvarez-Ortega, S. & Peña-Santiago, R. 2012. Nematodes of the order Dorylaimida from Andalucía Oriental, Spain. Nevadanema nevadense gen. n., sp. n. (Qudsianematidae) from Sierra Nevada National Park. Nematology 14: 249-264. Andrássy, I. 1959. Taxonomische Ubersicht der Dorylaimen (Nematoda). I. Acta Zoologica Academiae Scientiarum Hungaricae 5: 191-240. Andrássy, I. 1962. Nematologische Notizen, 11. Opuscula Zoologica Instituti Zoosystematici Universitatis Budapestinensis 4: 9-19. Andrássy, I. 2002. Free-living nematodes from the Ferto-Hanság National Park, Hungary. In: The Fauna of the Ferto-Hanság National Park (S. Mahunka Ed.) pp. 21-97. Akadémiai Kiadó, Budapest. Bastían, H.C. 1865. Monograph on the Anguillulidae, or free nematoids, marine, land, and freshwater; with descriptions of 100 new species. Transactions of the Linnean Society of London - Zoology 25: 73-184.
Blaxter, M.L., De Ley, P., Garey, G.R., Liu, L.X., Scheldeman, P., Vierstraete, A., Vanfleteren, J.R., Mackey, L.Y., Dorris, M., Frisse, L.M., Vida, J.T. & Thomas, W.K. 199S. A molecular evolutionary framework for the phylum Nematoda. Nature 392: 71-75.
Bongers, T. 19SS. De Nematoden van Nederland. The Netherlands, Utrecht, KNNV. 40S pp.
Choi, Y.E., Geraert, E. & Baek, H.S. 1997. Taxonomic study of Dorylaimoidea (Nematoda: Dorylaimida) from Korea. Korean Journal of Applied Entomology 36: 1-29.
De Grisse, A. 1969. Redescription ou modifications de quelques techniques utililisées dans l'étude des nématodes phytoparasitaires. Mededelingen van de Rijksfaculteit Landbouwetenschappen Gent 34: 351369.
HALL, T.A. 1999. BioEdit: A user-friendly biological sequence alignment and analysis program for Windows 95/9S/NT. Nucleic Acid Symposium Series 41: 95-9S.
HOLTERMAN, M., VAN DER WURFF, A., VAN DEN ELSEN, S., van Megen, H., Bongers, T., Holovachov, O., BAKKER, J. & HELDER, J. 2006. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution 23: 1792-1S00.
Holterman M., Holovachov O., van den Elsen S., VAN MEGEN H., BONGERS T., BAKKER J. & HELDER J. 200S. Small subunit ribosomal DNA-based phylogeny of basal Chromadoria (Nematoda) suggests that transitions from marine to terrestrial habitats (and vice versa) require relatively simple adaptations. Molecular Phylogenetics and Evolution 4S: 75S-763.
JIMÉNEZ-GUIRADO, D. 19S9. Nematodos acuáticos del Parque Nacional de Doñana. Oxyura 5: S3-91.
Loof, P.A.A. & Grootaert, P. 19S1. Redescription of Labronema vulvapapillatum Meyl, (1954) nov. comb. (Dorylaimoidea). Nematologica 27: 139-145.
McCarthy, C. 1997. Chromas, Version 1.41, Griffith University, Brisbane.
Meldal, B.H.M., Debenham, N.J. , De Ley, P., TANDINGAN DE LEY, I., VANFLETEREN, J., VIERSTRAETE, A., Bert, W., Borgonie, G., Moens, T., Tyler, P.A., Austen, M.C., Blaxter, M., ROGERS, A.D. & LAMBSHEAD, P.J.D. 2007. An improved molecular phylogeny of the Nematoda with special emphasis on marine taxa. Molecular Phylogenetics and Evolution 42:622-636.
MEYL, H. 1954. Die bisher in Italien gefundenen freilebenden Erd und Süsswasser-Nematoden. Archivo di Zoologia Italiano, Torino 39: 161-264.
MULLIN, P.G., HARRIS, T.S. & POWERS, T.O. 2005. Phylogenetic relationships of Nygolaimina and
Dorylaimina (Nematoda : Dorylaimida) inferred from small subunit ribosomal DNA sequences. Nematology 7:59-79.
Murillo-Navarro, R. & Jimenez-Guirado, D. 2006. Some dorylaimid nematodes from coastal dunes in Cadiz Bay (SW Spain). Journal of Nematode Morphology and Systematics 8: 161-178.
Oliviera, C.M.G., Hübschen, J., Brown, D.J.F., Ferraz, L.C.C.B., Wright, F. & Neilson, R. 2004. Phylogenetic relationships among Xiphinema and Xiphidorus nematode species from Brazil inferred from 18S rDNA sequences. Journal of Nematology 36: 153-159.
Page, R.D.M. 1996. TreeView: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357-358.
Pedram, M., Niknam, G., Guerrero, P., Ye, W. & Robbins, R.T. 2009. Morphological and molecular characterization of Enchodelus babakicus n. sp. and E. macrodorus Thorne, 1939 (Dorylaimida: Nordiidae) from Iran. Nematology 11: 895-907.
Ronquist, F. & Huelsenbeck, J. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572-1574.
Thorne, G. 1939. Notes on free-living and plant-parasitic nematodes. V. Proceedings of the Helminthological Society of Washington 6: 30-32.
E. Shokoohi, J. Abolafia, A. Mehrabi-Nasab, R. Peña-Santiago. К идентификации вида Labronema vulvapapillatum (Meyl, 1954) Loof & Grootaert, 1981 (Dorylaimida, Qudsianematidae). Резюме. Проведено всестороннее изучение популяций Labronema vulvapapillatum из Ирана и получены молекулярные и морфологические данные. Нематоды характеризуются длиной тела самок 1.76-2.50 мм и самцов 1.55-2.19 мм, обособленным заметным углублением губным отделом шириной 19-22 мкм у самок и 20-24 мкм у самцов, одонтостилем длиной 22-26 мкм у самок и 2131 мкм у самцов, то есть равным или слегка превышающим (1.0-1.3 раза) ширину губного отдела, шейным отделом длиной 379-495 мкм у самок и 366-484 мкм у самцов, расширением пищевода длиной 175-235 мкм у самок и 164-226 мкм у самцов или занимающим 43-49% длины шейного отдела, дидельфной половой системой самок, состоящей из трех частей маткой с короткой промежуточной частью, функционирующей как орган Z длиной 199-254 мкм, что в 2.6-4.0 раза больше диаметра тела, наличием pars refringens vaginae, продольной вульвой (V = 49-59), коротким закругленным хвостом (17-37 мкм длиной, c = 72-114, c' = 0.5-0.9 у самок; 19-28 мкм длиной, c = 59-82, c' = 0.6-0.8 у самцов), спикулами длиной 55-76 мкм и присутствием 16-25 смежных вентромедианных супплементов. Материал из Ирана сравнили с данными по другим известным популяциям и пришли к заключению, что существенных морфологических отличий не имеется, а главные морфометрические показатели широко перекрываются. Последовательность участка 18S рДНК иранской L. vulvapapillatum оказывается очень близкой к другим последовательностям, известным для Labronema, особенно к голландской популяции того же вида.
