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ACTA BIOMEDICA SCIENTIFICA, 2G18, Vol. 3, N 4
DOI: 10.29413/ABS.2018-3.4.25 УДК 579.62, 579.61
Corona B.G. Díaz-Sánchez A.A. Meli M.L. 2 3, Cañizares E.V. Arias L.R. Dorta Y.L.
Rivero E.L. Hofmann-Lehmann R. 2 3
OCCURRENCE OF TICK-BORNE PATHOGENS IN STRAY DOGS FROM HAVANA, CUBA
1 National Center for Animal and Plant Health (CENSA), Carretera de Tapaste y Autopista Nacional
(P.O.B. 10, CP 32700, San José de las Lajas, Mayabeque, Cuba) 2 Center for Clinical Studies, Vetsuisse Faculty, University of Zurich (Ramistrasse 71, Zürich CH-8006, Switzerland) 3 Clinical Laboratory, Vetsuisse Faculty, University of Zurich (Ramistrasse 71, Zürich CH-8006, Switzerland)
The article presents the results of examination of stray dogs from Havana, Cuba for six ixodid tick-borne diseases. Analysis was carried out using real-time PCR. Overall 107 dogs, 95 [89.09 0/o] were infected. 41 dogs [38.31 Ц], 66 [61.68 Ц], 28 [26.17 Ц] and 40 [37.38 Ц] were found to be infected with Anaplasma platys, Ehrlichia canis, Rickettsia spp. and Hepatozoon canis, respectively. Sequence analysis of 16S rRNA andgroEL genes for Rickettsia spp. revealed 99 Ц identity with Rickettsia felis. There were no dogs infected with A. phagocytophilum and Borrelia spp. Key words: tick-borne infections, dogs, Cuba
For citation: Corona B.G., Díaz-Sánchez A.A., Meli M.L., Cañizares E.V., Arias L.R., Dorta Y.L., Rivero E.L., Hofmann-Lehmann R. Occurrence of tick-borne pathogens in stray dogs from Havana, Cuba. Acta biomedica scientifica, 3 (4), 158-159, DOI 10.29413/ABS.2018-3.4.25.
ВСТРЕЧАЕМОСТЬ ВОЗБУДИТЕЛЕЙ КЛЕЩЕВОГО ЭНЦЕФАЛИТА У БРОДЯЧИХ СОБАК Г. ГАВАНА (КУБА)
Корона Б.Г. 1, Диас-Санчес А.А. 1, Мели М.Л. 2- 3, Канисарес Э.В. 1, Ариас Л.Р. 1, Дорта Й.Л. 1, Риверо Э.Л. 1, Хофманн-Леманн Р. 2- 3
1 National Center for Animal and Plant Health (CENSA), Carretera de Tapaste y Autopista Nacional
(P.O.B. 10, CP 32700, San José de las Lajas, Mayabeque, Cuba) 2 Center for Clinical Studies, Vetsuisse Faculty, University of Zurich (Rämistrasse 71, Zürich CH-8006, Switzerland) 3 Clinical Laboratory, Vetsuisse Faculty, University of Zurich (Rämistrasse 71, Zürich CH-8006, Switzerland)
В работе приведены результаты обследования популяции бродячих собак г. Гавана на заражённость шестью инфекциями, передаваемыми при укусах иксодовых клещей. Исследование проводили с помощью ПЦР сучётом результатов в режиме реального времени. Из 107 обследованных животных было заражено 95 [89,09 Ц]. Среди них 41 [38,31 Ц], 66 [61,68 Ц], 28 [26,17 Ц] и 40 [37,38 Ц] были заражены, соответственно, Anaplasma platys, Ehrlichia canis, Rickettsia spp. и Hepatozoon canis. Нуклеотидные последовательности генов 16S rRNA, gltA и htrA Rickettsia spp. были на 99 Ц идентичны последовательностям соответствующих генов Rickettsia felis, опубликованных в базе данных GenBank. Не было обнаружено случаев заражения собак A. phagocytophilum и Borrelia spp.
Ключевые слова: клещевые инфекции, собака, Куба
Для цитирования: Корона Б.Г., Диас-Санчес А.А., Мели М.Л., Канисарес Э.В., Ариас Л.Р., Дорта Й.Л., Риверо Э.Л., Хофманн-Леманн Р. Встречаемость возбудителей клещевого энцефалита у бродячих собак г. Гавана (Куба). Acta biomedica scientifica, 3 (4), 158-159, DOI 10.29413/ABS.2018-3.4.25.
Infectious diseases caused by pathogens transmitted by ticks and other vectors are an important cause of morbidity and mortality in both humans and dogs. Tickborne pathogens remain an important cause of disease among canine populations worldwide. In Cuba there are not many published studies on tick-borne pathogens affecting dogs, the only hemoparasite molecularly confirmed in dogs, is Anaplasma platys and in tick collected from dog's molecular detection of Ehrlichia canis and Babesia spp. in Rhipicephalus sanguineus sensu lato was published. The aim of this research was to screen, identify and characterize selected tick-borne pathogens in stray dogs from Havana, Cuba.
MATERIALS AND METHODS
Blood samples from 107 stray dogs with and without clinical signs associated with tick-borne diseases, were analyzed by real-time PCR for E. canis, A. platys, Anaplasma phagocytophilum, Borrelia spp., Hepatozoon canis and Rickettsia spp. Real-time PCR positive samples for different pathogens were further analyzed by PCR and sequencing.
RESULTS AND DISCUSSION
Overall 107 dogs, 95 (89.09 %) were positive for one or more hemoparasite and 12 (10.91 %) were not infected. None of the dogs were detected infected
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Brief reports
ACTA BIOMEDICA SCIENTIFICA, 2G18, Том 3, № 4
with A. phagocytophilum nor Borrelia spp., while 41 dogs (38.31 %), 66 (61.68 %), 28 (26.17 %) and 40 (37.38 %) were found to be infected with A. platys, E. canis, Rickettsia spp. and H. canis, respectively. Sequence analysis of 16S rRNA and groEL genes for A. platys, 16S rRNA and gltA for E. canis showed high identity with known sequences available in the GenBank database. In addition, htrA gene sequence analysis for Rickettsia spp. revealed 99 % identity with
Rickettsia felis. This first molecular study of different tick-borne pathogens in stray dogs points to the need of more intensive epizootic investigation, for pathogens with zoonotic potential.
Статья опубликована в рамках международной юбилейной конференции, посвящённой 20-летию научного сотрудничества между Россией и Монголией «Разные страны - общие проблемы природно-очаговых инфекций».
Information about the authors
Corona Belkis Gonzaleza - PhD, Senior Research Officer, National Center for Animal and PlantHealth (CENSA), Carretera de Tapaste y Autopista Nacional (Cuba, CP 32700, Mayabeque, San José de las Lajas, P.O.B. 10; e-mail: bcorona@censa.edu.cu)
Díaz Sanchez Adrian Alberto - MSc, Junior Research Officer, National Center for Animal and PlantHealth (CENSA), Carretera de Tapaste y Autopista Nacional (Cuba, CP 32700, Mayabeque, San José de las Lajas, P.O.B. 10; e-mail: adiaz@censa.edu.cu)
Meli Marina L. - PhD, Senior Research Officer at Clinical Laboratory, Vetsuisse Faculty, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich (Switzerland, CH-8006, Zürich, Ramistrasse, 71, e-mail: mmeli@vetclinics.uzh.ch)
Cañizares Ernesto Vega - PhD, Senior Research Officer, National Center for Animal and PlantHealth (CENSA), Carretera de Tapaste y Autopista Nacional (Cuba, CP 32700, Mayabeque, San José de las Lajas, P.O.B. 10; e-mail: evega@censa.edu.cu)
Arias Lisset Roblejo - Junior Research Officer, National Center for Animal and PlantHealth (CENSA), Carretera de Tapaste y Autopista Nacional (Cuba, CP 32700, Mayabeque, San José de las Lajas, P.O.B. 10; e-mail: lroblejo@censa.edu.cu)
Dorta Yanet López - MSc, Junior Research Officer, National Center for Animal and PlantHealth (CENSA), Carretera de Tapaste y Autopista Nacional (Cuba, CP 32700, Mayabeque, San José de las Lajas, P.O.B. 10; e-mail: ylopez@censa.edu.cu)
Rivero Evelin Lobo - PhD, Senior Research Officer, National Center for Animal and PlantHealth (CENSA), Carretera de Tapaste y Autopista Nacional (Cuba, CP 32700, Mayabeque, San José de las Lajas, P.O.B. 10; e-mail: elobo@censa.edu.cu)
Hofmann-Lehmann Regina - Professor, PhD, Senior Research Officer at Clinical Laboratory, Vetsuisse Faculty, Center for Clinical Studies, Vetsuisse Faculty, University of Zurich (Switzerland, CH-8006, Zürich, Ramistrasse, 71, e-mail: rhofmann@vetclinics.uzh.ch)
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