CC BY
17B-I-20
Observation of osmotically induced strain in biological tissues with optical coherence elastography
Yu.M. Alexandrovskaya1, O.I. Baum1, A.A. Sovetsky2, V.Yu. Zaitsev2
1 Institute of Photon Technologies, Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Troitsk, 108840 Moscow, Russia
2 Institute of Applied Physics of the Russian Academy of Sciences, 603950 Nizhny Novgorod, Russia
yu.alexandrovskaya@gmail.com
Application of non-isotonic solutions such as contrast and clearing agents for optical diagnostics of biological tissues produces strain gradients with an amplitude depending on the nature and concentration of the agent and permeability of a particular tissue [1,2]. Direct non-contact and realtime observation of the osmotically induced strains has been limited by the lack of instrumentation. Here, we present a new elasrographic technique enabling 2D visualization of depth distribution of such strains and their dynamics during the agent diffusion. Additionally, thus monitored strain dynamics may provide information about the biological matrix hydration and permeability. The ability of the technique to reveal alterations in the tissue structure and integrity is demonstrated.
References
[1] Yu. Alexandrovskaya, O. Baum, V. Zaitsev, A. Sovetsky, A. Matveyev, L. Matveev, K. Larin, E. Sobol, V. Tuchin, Optical and mechanical properties of the cartilage during optical clearing, In book Tissue optical clearing: new prospects in optical imaging, CRC Press (Dan Zhu, Elina Genina, and Valery Tuchin Eds.), 2021, Boca Raton, Florida, United States
[2] Yu. M. Alexandrovskaya, O.I. Baum, A.A. Sovetsky, L.A. Matveev, A.L. Matveyev, and V.Yu. Zaitsev, Relaxation and osmotic-induced slow strain mapping in biological tissues by optical coherence elastography, Proc. SPIE 11845, Saratov Fall Meeting 2020: Optical and Nanotechnologies for Biology and Medicine, 1184503; https://doi.org/10.1117/12.2590710.
