CC BY
0The 30th International Conference on Advanced Laser Technologies B-I-25
ALT'23
Nonlinear optical microscopic imaging techniques and applications
Liwei Liu1
1- Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, No. 3688, Nanhai Avenue, Shenzhen 518060, China.
Main author email address: liulw@szu.edu.cn
Ensuring the health of individuals is a key imperative highlighted in the 14th Five-Year Plan for National Development. How to meet this demand presents a formidable challenge for scientists engaged in information science and related fields. Currently, the field of biomedical photonics has emerged as a branch of rapidly advancing information science, while also serving as an integral component of optical engineering and medical imaging. The rapid advancement and breakthroughs in this field are attributed to the support of optical imaging technology, particularly optical microscopic imaging technology.
In the past two decades, optical microscopic imaging technology has undergone rapid development and constant breakthroughs, providing a crucial tool for real-time dynamic observation of life systems and facilitating the transformation of optical microscopy from scientific research to clinical application. However, in the face of rapid technological development and increasing demand, it remains imperative to continuously explore and develop novel methods and technologies for addressing bottleneck issues encountered during practical applications of optical microscopic imaging, such as limited resolution, information acquisition, and imaging speed. Our research group has conducted fundamental and applied research in the development and application of optical microscopic imaging technology for over a decade. The content encompasses a range of nonlinear optical microscopic imaging techniques, including fluorescence lifetime, two-photon excited fluorescence, second harmonic generation, stimulated Raman scattering, and others. These techniques enable imaging characterization at various levels spanning from molecules to cells, tissues, and in vivo. This report primarily presents the recent research endeavors of our research group in utilizing nonlinear optical microscopy imaging technology for biomedical applications.
