CC BY
10B-O-9
BIOMEDICAL PHOTONICS
NIR responsive Fe doped nanodiamond for Fenton enhanced chemotherapy
RSelvam1, W.G.Pearl1, E.Prevedentseva1'2, A.Karmenyan1, C.L Cheng1*
1 Department of Physics, National Dong Hwa University, Hualien 97401, Taiwan 2 P. N. Lebedev Physics Institute of Russian Academy of Sciences, Moscow, 119991, Russia Presenting author: 810714313@gms.ndhu.edu.tw; corresponding author: clcheng@gms.ndhu.edu.tw
Chemodynamic therapy (CDT) based on Fenton or Fenton-like reactions is an emerging technique that has made significant progress in cancer treatment by efficiently fighting cancer while reducing negative effects on normal cells and tissues. Cancer cells exhibit elevated levels of hydrogen peroxide [1], which can be utilized to catalyze the Fenton reaction leading to the formation of reactive oxygen species (ROS) leading to cell death or apoptosis. Taking advantage of tumor microenvironment, we used Fe doped nanodiamond (FeND) as a therapeutic agent for cancer by using Fenton reaction. FeND exhibits excellent biocompatibility against A549 cells, however, when exposed to laser light, the nanodiamond exhibits significant toxicity. In the presence of NIR laser light, FeND can undergo a redox reaction with endogenous H2O2 in the cells to produce highly toxic hydroxyl radicals (•OH). Upon production of an hydroxyl radical in a cell, it can reacts with biological system components and causes the cell to undergo apoptosis. However, endogenous H2O2 is rather insufficient to effectively treat cancer. We have therefore conjugated doxorubicin (DOX), a commercially available anthracycline drug, which can undergo redox reaction to generate excess amounts of H2O2 inside the cells [2], which can then be converted into hydroxyl radicals by FeND. The effectiveness of the FeND and FeND-DOX complex have been studied with and without exposure to the NIR laser against A549 cells using MTT assay. Based on our findings, FeND has a potential to be used as a biological probe as well as a carrier for drug, along with it's ability to treat cancer.
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Figure 1. FLIM images of (a) A549 (b) A549 incubated with Fe-ND for 4 hrs. (c) Lifetime decay curves of Fe-ND fluorescence and A549 cancer cells autofluorescence. (d) cytotoxicity of DOX and Fe-ND-DOX complex evaluated in A549 cells with and without exposure irradiation with laser of 808 nm
[1] T.P.Szatrowski, and C.F. Nathan, Production of large amounts of hydrogen peroxide by human tumor cells. Cancer Res, 51(3): p. 794-8, 1991
[2] H. Mizutani, S.Tada-Oikawa, Y. Hiraku, M.Kojima and S.Kawanishi, Mechanism of apoptosis induced by doxorubicin through the generation of hydrogen peroxide. Life Sci,. 76(13): p. 1439-53, 2005.
