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Protistology 6 (2), 111-125 (2009)
Protistology
New testate amoebae (Protozoa, Testacea) from the Northern-East Azerbaijan inland water bodies
Nataly Snegovaya and Ilham Alekperov
Laboratory of Protistology, Institute of Zoology, Azerbaijan National Academy of Sciences, Baku, Azerbaijan
summary
Testate amoebae of the Northern-East Azerbaijan several small inland water bodies have been investigated. In the present paper descriptions of the new genus and 12 new species ( Centropyxispileformis sp. n., C. trigonostoma sp. n., C. pectinata sp. n., Difflugiacrucistomasp. n., D. immemoratasp. n., D. khachmazicasp. n., Lesquereusia nabranica sp. n., L. contorta sp. n., L. azerbaijanica sp. n., L. macrolabiata sp. n., Shamkiriella turanica sp. n., Nabranella brevis gen.et sp. n.) have been given.
Key words: testate amoebae, freshwater, fauna of Azerbaijan
Introduction
The aquatic testate amoebae of the Northern-East Azerbaijan inland water bodies were not investigated. This region not far from state border with Russia has numerous shallow small rivers and spring waters mainly with clean fresh water. In the present paper we have shown the results of our two years investigations the fauna of testate amoebae of freshwater plankton, periphyton and sediments this region rivers and spring waters.
Material and methods
We have studied the fauna of testate amoebae in the several small rivers and spring waters of Northern-East Azerbaijan near Russian borders. In 2004-2007 more than 60 samples of freshwater plankton, periphyton and benthos were collected and processed. Testate amoebae were caught by microcapillars and studied basically in vivo. The type material all new taxa has been deposites in the col-
lection of the Protistology laboratory in the Institute of Zoology NAS of Azerbaijan, Baku city.
Statistics were performed using the program SigmaStat 2.0 ( X — arithmetic mean, M — median, SD — standard deviation, SE — standard error, CV— coefficient of variance (%), Max — maximum, Min
— minimum, n — number of specimens).
Results
Centropyxis pileformis sp. n. (Fig. 1, A-C; Plate 1, A-C)
Diagnosis. Shell 165-180 ^m x 140-150 ^m. Shell hat-like shape laterally with three different size oval cavity along shell S size perimeter. Usually 2-3 lobose pseudopodia. Freshwater.
Type specimens. One holotype as a slide N-E-4 has been deposited.
Type location. Sediments in the spring water near Nabran village.
Description. The shell is oval in ventral view and
© 2009 by Russia, Protistology
Table 1. Biometric characteristic of Centropyxis pileformis sp. n.
Character X M SD SE CV Min Max n
Length of shell 173.0 173.0 4.922 1.556 0.028 165.0 180.0 10
Breadth of shell 144.90 145.0 4.254 1.345 0.029 140.0 150.0 10
Height of shell 86.0 86.0 4.055 1.282 0.047 80.0 90.0 10
Length of aperture 76.6 73.0 2.366 0.748 0.031 70.0 75.0 10
Breadth of aperture 65.4 65.0 1.578 0.499 0.024 63.0 67.0 10
Length of spines 22.4 23.0 2.221 0.702 0.099 20.0 25.0 10
hat-like flattened laterally. Length of shell 165-180 ^m, breadth 140-150 ^m, height 80-90 ^m. In the middle of the ventral side there is a large trapezoidal aperture (70-75 x 63-67 ^m). The shell usually has 2 lateral spines (20-25 ^m long). There are 3 lateral cavities. All shell surface covered with inorganic particles of different shape and size. Endoplasm colorless. Pseudopodia are lobose, usually 2-3. There are 1-3 contractile vacuoles.
Comparision with related species. There are only two Centropyxis species more or less morphologically similar to C. pileformis sp. n.. First of them C. recurvata Vucetich, 1976, which has similar body shape from lateral view, but clearly differs from C. pileformis by bigger shell size (165-180 ^m versus 200-230 ^m), twice more aperture size (70-75 ^m versus 135-150), its shape and location (C. pileformis has aperture in the middle of the ventral side). From C. cordobensis Vucetich, 1976 our C. pileformis sp. n. clearly differs by smaller shell size (165-180 ^m versus 260-285 ^m), height of shell (80-90 ^m versus 200-210 ^m) and breadth ofshell (140-150 ^m versus 190-210 ^m). C. pileformis has two lateral spines and lateral cavities which absence in C. cordobensis.
Etymology. The species name refers to hat-like shell morphology.
Centropyxis trigonostoma sp. n. (Fig. 1, D-E; Plate 1, D-F)
Diagnosis. Uncommon shell shape with well developed ventral torsion below aperture and two spherical anterior cavities on left and right sides from aperture.
Type specimens. One holotype as a slide N-E 1 has been deposited.
Type location. Sediments and surface aquatic plants in the spring water near Nabran village.
Description. Length of shell 70-80 ^m, breadth 80-90 j^m. Shell shape with well developed triangular torsion below aperture and rounded anterior top. The aperture bordered by a thin lip well visible from ventral side. There are two spherical anterior cavities on left and right side from aperture. The shell surface revetment with sand grains of different shape. Endoplasm grey. Pseudopodia are lobose, usually 1-2. There are 3-6 contractile vacuoles.
Comparison with related species. Considering its overall appearance, C. trigonostoma sp. n. should be compare with closely related by shell shape and sizes C. hirsuta Deflandre, 1929, C. cassis spinifera (Playfair, 1918) Deflandre, 1929, C. aculeata minima Van Oye, 1958, C. decloitrella Laminger, 1973 (Table 4). C. trigonostoma sp. n. is easily distinguished from the remaining known species of the genus Centropyxis by its uncommon shell shape, arrangement aperture and its biometric characteristics (Table 2).
Etymology. The species name refers to its aperture shape.
Centropyxispectinata sp. n. (Fig. 2, A-B; Plate 1, G, 2, A)
Diagnosis. Shell with characteristic anterior top spine and cone-like shape from apertural view.
Type specimens. One holotype as a slide N-E 2 has been deposited.
Table 2. Biometric characteristic of Centropyxis trigonostoma sp. n.
Character X M SD SE CV Min Max n
Length of shell 75.8 75.0 4.050 1.281 0.053 70.0 80.0 10
Breadth of shell 85.7 86.0 3.831 1.212 0.045 80.0 90.0 10
Length of spines 17.7 18.0 2.263 0.716 0.128 15.0 20.0 10
Fig. 1. A-C — Centropyxis pileformis sp. n.: A — ventral, B — aboral, C — lateral side; D-E — C. trigonostoma sp. n.: D — ventral, E — apical side. Scale bars: A-C — 50 цш; D-E — 20 цш.
Type location. Sediments in the spring water near Nabran village.
Description. Shell triangular anteriorly with rounded aboral part. Shell length is 100-120 ^m, breadth — 50-60 ^m. From ventral side the shell
outline is ovoid with characteristic apical top spine. Oval aperture sizes 30-40 x 45-50 ^m. Shell has 6-7 lateral spines. Its length — 25-30 ^m. Endoplasm grey, long blade-shape 1-2 pseudopodia are rarely observed.
Table 3. Biometric characteristic of Centropyxis pectinata sp. n.
Character X M SD SE CV Min Max n
Length of shell 109.0 107.5 8.433 2.667 0.077 100.0 120.0 10
Breadth of shell 56.0 57.5 4.595 1.453 0.082 50.0 60.0 10
Length of aperture 35.5 35.0 4.378 1.384 0.123 30.0 40.0 10
Breadth of aperture 49.9 50.0 3.985 1.260 0.08 45.0 50.0 10
Depth of aperture 18.8 20.0 3.011 0.952 0.160 15.0 22.0 10
Length of spine 28.2 30.0 2.394 0.757 0.085 25.0 30.0 10
Plate 1. A-C — Centropyxis pileformis sp. n., A — ventral, B — aboral, C — lateral side; D-F — C. trigonostoma sp. n., D — ventral, E — apical, F — aboral side; D — C. pectinata sp. n., ventral side. Scale bars: A-C — 50 цш; D-G — 20 цш.
Comparison with related species. C. pectinata sp. n. distinctly differs from the other species of the Centropyxis genus by its shell shape, arrangement of a characteristic anterior top spine, presence of dorsal ones and by its biometric characterization (Table 3). Comparison C. pectinata sp. n. with related species are given in the Table 5.
Etymology. The species named refers Lat. “pectinatus” — pectinate.
Difflugia crucistoma sp. n. (Fig. 2, C-D; Plate 2, B-C)
Diagnosis. Shell elongate, flattened laterally. Apical aperture has invariable four blades without collar. Freshwater.
Type specimens. One holotype as a slide N-E 7 has been deposited.
Type location. Sediments in the spring water near Khudat city.
Description. The shell elongate, flattened laterally (85-95 x 65-75 ^m). Apical aperture without collar and has constant four blades shape. Aperture size — 15-20 ^m. The shell surface covered with a small siliceous particles. The endoplasm bright brown. There are 3-5 short lobopodia.
Comparison with related species. According to the sub-division of Gauthier-Lievre and Thomas (1958), D. crucistoma sp. n. belongs to the loboses group ofthe Difflugia genus. D. crucistoma should be compared with closely related species of this group
— D. gramen Penard, 1902, D. lismorensis Playfair,
Table 4. Comparison Centropyxis trigonostoma sp. n. with related species.
Species Characters
Length of shell (Mm) Breadth of shell (Mm) Aperture shape and size (^m) Shape of shell Arrange ment of spines Data source
C. trigonostoma sp. n. 70-80 80-90 0 35-40 Triangular in above view; oval in ventral view; bordered by a thin lip Ovoid or circular ventrally, laterally hemispherical Laterally Original
C. hirsuta Deflandre, 1929 72-88 72-88 0 33-50 Invaginated, oval, sub-terminal Usually circular ventrally, laterally hemispherical All shell surface Ogden and Hedley, 1980; Mazei and Tsyaganov, 2006
C. cassis spinifera (Playfair, 1918) Deflandre, 1929 72-87 --- 0 31-49 Invaginated, oval, subterminal, has a pronounced rim Spherical in the aboral region and tapers towards the aperture Laterally Deflandre, 1929; Mazei and Tsyaganov, 2006
C .aculeata minima Van Oye, 1958 82-85 82-85 0 38-39 Oval-like shape, subterminal Spherical and tapers towards the aperture laterally Van Oye, 1958; Mazei and Tsyaganov, 2006
C. decloitrella Laminger, 1973 81 71 0 35 Oval-like shape with rough margins Spherical or subspherical ventrally, flattened laterally On the adoral and aboral ends Laminger, 1973; Mazei and Tsyaganov, 2006
1918, D. lobostoma Leidy, 1879, D. papillomata Gauthier-Lievre et Thomas, 1958 and D. schurmanni Van Oye, 1932. Comparison D. crucistomasp. n. with related species are given in the Table 7. D. crucistoma sp. n. differs from others species by its flattened laterally shell and invariable aperture four blades.
Etymology. The species named refers Lat. “cruci”
— cross and “stoma” — mouth.
Difflugia immemorata sp. n. (Fig. 3, A; Plate 3, A)
Diagnosis. Shell drop-shape and with rough surface neck consists of small size particles. Shell surface covered with a big different form sand particles, which brake oval outline. Freshwater.
Type specimens. One holotype as a slide N-E 10 has been deposited.
Table 5. Comparison Centropyxis pectinata sp. n. with related species.
Species Characters
Length of shell (Mm) Breadth of shell (Mm) Aperture shape and size (Mm) Shape of shell Spines number and sizes Data source
C. pectinata sp. n. 100-120 50-60 30-40 x 45-55 Ovoid ventrally Shell triangular anteriorly with rounded aboral part 6-7 Lateral spines with characteristic apical top one; 2530 |jm Original
C. decloitrella Laminger, 1973 81 71 0 35 Oval, strong invaginated, aperture rim is rough. Spherical or subspherical, flattened laterally 9 Lateral spines along shell outline Laminger, 1973; Mazei and Tsyaganov, 2006
C. aculeata (Ehrenberg, 1830) Stein, 1857 92-178 77-137 0 35-70 Ovoid or circular ventrally. In lateral view - spherical and tapers towards the aperture 9 Lateral spines; 1240 |jm Ehrenberg, 1830; Ogden and Hedley, 1980; Mazei and Tsyaganov, 2006
Fig. 2. A, B — Centropyxis pectinata sp. n.: A — ventral, B — lateral side; C, D — Difflugia crucistoma sp. n.: C — apical end; D — lateral side. Scale bars: A-B — 20 цш; C-D — 25 цш.
Type location. On the aquatic plants surface in the small river near Nabran village.
Description. Length of shell, 80-90 ^m, breadth, 60-70 ^m. Shell drop-shape. Anterior aperture (1520 ^m) circular. Short neck consists with small size sand particles. Other surface covered with bigger size particles. Endoplasm grey. There are 1-3 short lobopodia.
Comparison with related species. Our D. imme-morata sp. n. differs from closely related Difflugia pyriformella Chardez, 1982 (Chardez, 1982) smaller size (80-90 versus 100-125 ^m) and low neck.
Etymology. The species name refers Lat. “imme-moratus” — new, unknown earlier.
Table 6. Biometric characteristic of Difflugia crucistoma sp. n.
Character X M SD SE CV Min Max n
Length of shell (in dorsal view) 89.0 90.0 3.944 1.247 1.247 85.0 95.0 10
Breadth of shell (in apertural view) 70.5 70.0 4.378 1.384 1.384 65.0 75.0 10
Length of blade 17.9 18.5 2.331 0.737 0.737 15.0 20.0 10
Plate 2. A — Centropyxis pectinata sp. n.: lateral side; B, C — Difflugia crucistoma sp. n.: B — lateral; C — apical side. Scale bars: A — 20 цш; B-C — 25 цш
Difflugia khachmazica sp. n. (Fio. 3, B-C; Plate 3, river near Khachmaz city.
B-C) Description. Length of shell 90-100 цш, breadth,
50-б5 цш. Subapical aperture (15-20 цш) usually Diagnosis. Shell bean-shape, small curved tetragonal shape. Shell shape bean-like small curved
anterially. Subapical aperture with four-blade to anteriorly. All shell surface covered of various shape
tetragonal form. Freshwater.
particles. Endoplasm transparent, often light green.
type specimens. One holotype as a slide N-E 15 Lobopodia (1-2) observed rarely.
has been deposited.
Comparison with related species. Our D. khachmazica
type location. On the sediments in the small sp. n. differs from closely related D. bryophila (Penard, Table 7. Comparison Difflugia crucistoma sp. n. with related species.
Species Characters
Total length (Mm) Width (Mm) Shell outline Diameter and aperture shape Data source
D. crucistoma sp. n. 85-95 65-75 Elongated, flattened laterally 0 15-20 Apical, without collar, has 4 blades shape Original
D. gramen Penard, 1902 60-117 47-112 Spherical or ovoid 0 17-39 Trilobed, surrounded by a slightly raised collar of small particles Penard, 1902; Gauthier-Liévre and Thomas, 1958; Ogden and Hedley, 1980; Mazei and Tsyaganov, 2006
D. lismorensis Playfair, 1918 115-145 70-135 Ovoid 0 36-45 3-7 lobes identical by form, strong and blunt, not collar Playfair, 1918; Gauthier-Liévre and Thomas, 1958; Mazei and Tsyaganov, 2006
D. lobostoma Leidy, 1879 88-186 68-166 Spherical or ovoid, rounded in transverse section 0 28-60 3-4 blades, surrounded by collar Leidy, 1879; Gauthier-Liévre and Thomas, 1958; Mazei and Tsyaganov, 2006
D. papillomata Gauthier-Lievre et Thomas, 1958 105 68 Linear-ovoid 0 30 5-blades, surrounded well visible, transparent, long collar Gauthier-Liévre and Thomas, 1958; Mazei and Tsyaganov, 2006
D. schurmanni Van Oye, 1932 50-85 36-69 Linear-egg-shaped, narrow from middle to aperture direction 0 18-30 3 small blades Van Oye, 1932; Gauthier-Liévre and Thomas, 1958; Mazei and Tsyaganov, 2006
Fig. 3. A — Difflugia immemorata sp. n.: lateral side; B, C — D. khachmazica sp. n.: B — apical end; C — lateral side; D — Lesquereusianabranica sp. n.: lateral side; E — L. contorta sp. n.: lateral side. Scale bars: A — 10 цш; B-C — 20 цш; D-E — 50 цш.
1902) (Gauthier-Liévre, Thomas, 1958; Boltovskoy, Lena, 19У4) by shell structure and other aperture shape.
etymology. The species named after region found (Khachmaz district).
At the present time, before our investigations the genus Lesquereusia Schlumberger, 1845 contains 8 species — L. combinata (Stepänek, 1952), Bartos, 1954, L. epistomium Penard, 1893, L. inequalis Cash et Hopkinson, 1909, L. longicollis Dekhtyar, 1994, L. longicollis depressa Dekhtyar, 1994, L.
modesta Rhumbler, 1895, L. spiralis (Ehrenberg, 1840) Butschli, 1888, L. mimetica Penard, 1911. Comparison described below our four new species with other known Lesquereusia representatives are given in the Table 10.
Lesquereusia nabranica sp. n. (Fig. 3, D; Plate 3, D)
Diagnosis. Retort shape shell with rather long curved neck. Shell surface covered with uniform thin elongate siliceous particles. Freshwater.
Table 8. Biometric characteristic of Centropyxis pectinata sp. n.
Character X M SD SE CV Min Max n
Length of shell 85.5 85.0 4.378 4.378 0.051 80.0 90.0 10
Breadth of shell 65.0 65.0 4.714 4.714 0.073 60.0 70.0 10
Diameter of aperture 17.7 18.5 2.497 2.497 0.141 15.0 20.0 10
Length of collar 15.2 15.0 1.476 1.476 0.097 13.0 17.0 10
Plate 3. A — Difflugia immemorata sp. n.: lateral side; B, C — D. khachmazica sp. n.: B — lateral, C — apical side; D — Lesquereusia nabranica sp. n.: lateral side; E — L. contorta sp. n.: lateral side; F — L. azerbaijanica sp. n.: lateral side; G — L. macrolabiata sp. n.: lateral side; I, J — Shamkiriella turanica sp. n.: I — lateral, J — dorsal side. Scale bars: A — 10 ^m; B-C, G — 20 ^m; D-F, I-J — 50 ^m.
Type specimens. One holotype as a slide N-E 7 has been deposited.
type location. Sediments and aquatic plants surface in the spring water near Nabran village.
Description. Shell length is 145-160 ^m, width is 100-120 ^m. Shell retort shape with a long a little curved neck. Apical spherical aperture (25-35 ^m) has a thin lips. All shell covered with
Table 9. Biometric characteristic of Difflugia khachmazica sp. n.
Character X M SD SE CV Min Max n
Length of shell 93.5 92.5 4.116 1.302 0.044 90.0 100.0 10
Breadth of shell 58.0 6.0 6.325 2.0 0.109 50.0 65.0 10
Diameter of aperture 17.4 17.0 2.366 0.748 0.136 15.0 20.0 10
Species Characters
Length of shell (Mm) Breadth of shell (Mm) Form and diameter of aperture (Mm) Shell shape Shell composition Neck Data source
L. combinata (Stëpânek, 1952), Bartos, 1954 150-175 70-80 0 40 Shell consist of spherical fundus and wide neck Composed of mixture of curved siliceous rods and irregular grins of quartz Unsymmetrical, slightly flattened laterally Stëpânek, 1952; Bartos, 1954; Mazei and Tsyaganov, 2006
L.epistomium Penard, 1902 90-70 55-60 0 37. Roughly circular and surrounded by siliceous rods Ovoid or circular with adistinct long neck Composed of curved, siliceous rods and the occasional quartz particle The neck is joined to the body at the shell at an angle, so that it has one short and one long side Penard, 1902; Ogden, Hedley, 1980; Mazei and Tsyaganov, 2006
L. inequalis Cash et Hopkinson, 1909 135 125 — From lateral view incorrectly triangular form Curved rods Short Cash et Hopkinson, 1909; Mazei and Tsyaganov, 2006
L. longicollis Dekhtyar, 1994 132-158 102-122 0 23-40 From lateral view flattened, fundus ellipsoidal laterally Composed of short curved sand siliceous rods and large particles High, unsymmetrical Dekhtyar, 1994; Mazei and Tsyaganov, 2006
L. longicollis depressa Dekhtyar, 1994 125-145 109-120 0 29-46 As in L. longicollis As in L.longicollis Short, unsymmetrical Dekhtyar, 1994; Mazei and Tsyaganov, 2006
L. modesta Rhumbler, 1895 100-174 100-159 0 25-43. Terminal, circular and bordered by siliceous rods or small particles of quartz Circular with an unsymmetrical neck; slightly flattened laterally Siliceous rods and grains of quartz Unsymmetrical Rhumbler, 1895; Ogden, Hedley, 1980; Mazei and Tsyaganov, 2006
L. spiralis (Ehrenberg, 1840) Bütschli, 1888 90-150 86-109 0 40. Terminal, circular and bordered by siliceous rods Circular or ovoid with an unsymmetrical neck, slightly flattened laterally Siliceous curved rods interspersed with a meshwork of organic cement Unsymmetrical Ehrenberg, 1840; Ogden and Hedley, 1980; Mazei and Tsyaganov, 2006
L. mimetica Penard, 1911 370-460 280-350 Oval laterally, neck bent back on body, forming oblique internal partition Siliceous particles Bent back on body, forming oblique internal partition Penard, 1911; Thomas and Gauthier-Liévre, 1959
L. nabranica sp. n. 145-160 100-120 0 25-35. Apical spherical, with a thin lips Retort shape with rather long curved neck Uniform thin elongate siliceous rods Long curved Original
L. contorta sp. n. 145-160 90-100 0 25-35. Apical aperture with thin lips Pear-shape form with straight neck and slightly flattened ventrally Uniform thin elongate, usually slightly curved siliceous rods Straight Original
L. azerbaijanica sp. n. 85-105 63-75 0 25-35. Apical aperture Retort-shaped with cone-like neck Different shape particles Long, cone-like Original
L. macrolabiata sp. n. 90-100 105-120 0 15-20. Apical spherical aperture has a well developed collar Retort-shape, cone-like body neck, joined to the body of the shell at an angle Very thin and long rods Cone-like with uncommon collar Original
uniform thin elongate siliceous rods. Endoplasm colorless without inclusions usually light brown. Several contractile vacuoles and 2-5 short lobose pseudopodia.
Comparison with related species. Described above L. nabranica sp. n. differs from closely related L. epistomium Penard, 1902 and L. spiralis (Ehrenberg, 1840) by bigger shell size (145-1б0 versus 110-125
Character X M SD SE CV Min Max n
Length of shell 152.0 150.0 5.869 1.856 0.039 145.0 160.0 10
Breadth of shell 113.5 120.0 9.443 2.986 0.083 100.0 120.0 10
Diameter of aperture 30.0 30.0 4.714 1.491 0.157 25.0 35.0 10
- L. epistomium Penard, 1902 and 89-117 -
L. spiralis (Ehrenberg, 1840) according Ogden and Hedley, 1980) and other shell shape. Additionly from L. spiralis our L. nabranica clearly differs with long neck and fundus shape (Table 10).
Etymology. The species named after region found (Nabran village).
Lesquereusia contorta sp. n. (Fig. 3, E; Plate 3, E)
Diagnosis. Retort shape shell with straight neck. Apical aperture on the neck and has thin lips. All shell slightly flattened ventrally. Shell surface covered with uniform thin elongate siliceous rods. Freshwater.
Type specimens. One holotype as a slide N-E 14 has been deposited.
Type location. Peryphiton on the rocks in the small rivers near Nabran village.
Description. Shell length is 145-160 ^m, the width — 90-100 ^m. The shell constantly pear-shape with straight neck and slightly flattened ventrally. Apical aperture (25-35 ^m) has thin lips. Shell surface covered with uniform thin elongate, usually slightly curved siliceous particles. Endoplasm colorless light brown. Usually observed 1-5 lobopodia.
Comparison with related species. Described above species differs from closely related Lesquereusia nabranica sp. n. with straight neck aperture with lips and rather flattened ventrally shell shape (Table 10).
etymology. The species name refers Lat. “con-tortus” — curved.
Lesquereusia azerbaijanica sp. n. (Fig. 4, A; Plate 3, F)
Diagnosis. Retort shell shape typical for Lesquereusia genus. Neck is cone-shape with circular apical aperture. Freshwater.
Type specimens. One holotype as a slide N-E 12 has been deposited.
type location. Sediments and aquatic plants in the spring water near Nabran village.
Description. Shell length is 84-105 ^m, width
— 63-75 ^m. Shell retort-shaped with cone-like neck. Apical aperture 25-35 ^m size. The shell surface covered with short and wide, different shape particles. Endoplasm transparent usually light yellow.
Comparison with related species. Described above our species differs from closely related L. epistomium Penard, 1902 and L. spiralis (Ehrenberg, 1840) by smaller size (according Ogden and Hedley, 1980 — 130 ^m versus 84-105) and other shell structures (Table 10).
Etymology. The species named after region found (Azerbaijan Republic).
Lesquereusia macrolabiata sp. n. (Fig. 4, B; Plate
3, G)
Diagnosis. Common for Lesquereusia retortshape shell. The cone-like neck is joined to the fundus at an angle. Apical spherical aperture has a well developed collar. Freshwater.
type specimens. One holotype as a slide N-E 7 has been deposited.
type location. Peryphiton on the rocks in the small rivers near Nabran village.
Description. The shell length is 90-100 ^m, width is 105-120 ^m. Apical spherical aperture located on the cone-like neck. The siliceous rods, covered shell surface — very thin and long. Endoplasm colorless.
Table 12. Biometric characteristic of Lesquereusia contorta sp. n.
Character X M SD SE CV Min Max n
Length of shell 150.0 150.0 5.774 1.826 0.038 145.0 160.0 10
Breadth of shell 93.5 92.5 4.116 1.302 0.044 100.0 100.0 10
Diameter of aperture 30.5 90.0 4.378 1.384 0.144 25.0 35.0 10
Character X M SD SE CV Min Max n
Length of shell 93.8 94.5 6.844 2.164 0.073 84.0 105.0 10
Breadth of shell 67.0 67.0 3.771 1.193 0.056 63.0 75.0 10
Diameter of aperture 29.4 29.0 3.373 1.067 0.115 25.0 35.0 10
Comparison with related species. Our Lesque-reusia macrolabiata sp. n. clearly differs from closely related L. azerbaijanica with more thin and long shell surface rods. Additionly, from all other representatives of Lesquereusia genus differs of uncommon well developed collar round aperture (Table 10).
etymology. The species named refers Lat. “macro” — large and “labiatus” — lip.
Shamkiriella turanica sp. n. (Fig. 4, C-D; Plate 3,I-J)
In our previous article was described new genus Shamkiriella with 3 species (Snegovaya and Alekperov, 2005). In the present paper we described 4-th new species.
Diagnosis. Shell elongated, with characteristic longitudinal fold laterally flattened. Apical aperture oval shape with low-defined collar. The shell composed with transparent plates of variable size. Freshwater.
type specimens. One holotype as a slide N-E 10 has been deposited.
type location. Sediments in the spring water near Nabran village.
Description. Shell length is 160-180 ^m, breadth
- 80-95 ^m. Apical oval aperture (40-50 x 30-40 ^m) with low collar. Shell has typical for Shamkiriella genus approximately longitudinal folds. Endoplasm light green, granulate. Pseudopodia not observed.
Comparison with related species. Described above Sh. turanica sp. n. is a biggest representatives of Shamkiriella genus. Other three known species has more less sizes (Sh. convoluta — 100-115 ^m; Sh. reticulata — 125-140 ^m; Sh. phimatophora —
100-125 ^m). Besides Sh. turanica differs from other three species by low-defined aperture collar and other longitudinal folds arrangement.
etymology. The species named after region -“Turan” ancestrial name of South Caucasus and Middle Asia territories.
Nabranella gen. NOV.
Diagnosis. Shell light brown ovoid or spherical, without neck. Apical top flat with two holes aperture and transverse diaphragm — similar Pontigulasia genus. Our genus differs from closely related Pon-tigulasia by full neck absence. type species — Nabranella brevis gen et sp. n. Freshwater.
Nabranella brevis gen et sp. n. (Fig. 4, E-F; Plate
4, A,B)
Diagnosis. Shell oval or spherical shape without neck. Apical top flat and has transverse diaphragm with two holes aperture, very similar on the Ponti-gulasia aperture arrangement and clearly differs by neck absence.
type specimens. Holotype as a one slide N-E 6 has been deposited.
type location. Sediments in the spring water near Nabran village.
Description. The shell length is 80-90 ^m, breadth — 60-75 ^m. Neck absent. The shell composed of different size mineral particles. Apical top flat with two holes (40-50 ^m) and tranverse diaphragm. Endoplasm usually brown, pseudopodia not observed.
Comparison with related species. Our N. brevis gen et sp. n. differs from all closely related representatives
Table 14. Biometric characteristic of Lesquereusia macrolabiata sp. n.
Character X M SD SE CV Min Max n
Length of shell 94.8 95.0 4.131 1.306 0.044 90.0 100.0 10
Breadth of shell 113.7 115.0 5.498 1.739 0.048 105.0 120.0 10
Diameter of aperture 17.2 16.0 2.486 0.786 0.145 15.0 20.0 10
Height of collar 8.3 8.5 1.889 0.597 0.228 5.0 10.0 10
Fig. 4. A — Lesquereusia azerbaijanica sp. n.: lateral side; B —.L. macrolabiata sp. n.: lateral side; C, D — Shamkiriella turanica sp. n.: C — lateral, D — dorsal side; E, F — Nabranella brevis gen.et sp. n.: E — lateral side; F — apical view. Scale bars: A, C-D — 50 цш; B, E-F — 20 цш..
Plate 4. Nabranella brevis gen.et sp. n.: A — lateral,
B — apical side. Scale bar: 20 цш.
of Pontigulasia genus by full neck absence. In our opinion this difference has a genus level.
etymology. The species named refers Lat. “brevis” — short.
References
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Table 15. Biometric characteristic of Shamkiriella turanica sp. n.
Character X M SD SE CV Min Max n
Length of shell 170.0 170.0 8.498 2.687 0.05 160.0 180.0 10
Breadth of shell 87.0 85.0 7.149 2.261 0.082 80.0 95.0 10
Thickness of shell 56.5 57.5 4.116 1.302 0.073 50.0 60.0 10
Length of aperture 45.5 45.0 4.378 1.384 0.096 40.0 50.0 10
Breadth of aperture 36.0 35.0 3.944 1.247 0.11 30.0 40.0 10
Length of spine 7.8 7.0 2.044 0.646 0.262 5.0 10.0 10
Table 16. Biometric characteristic of Nabranella brevis gen et sp. n.
Character X M SD SE CV Min Max n
Length of shell 86.2 86.0 3.938 1.245 0.046 80 90 10
Breadth of shell 69.5 70.0 4.378 1.384 0.063 60.0 75.0 10
Depth of aperture 17.4 17.0 2.366 0.748 0.136 15.0 20.0 10
Diameter of aperture 46.0 45.0 3.944 1.247 0.086 40.0 50.0 10
Length of bottom aperture 15.2 15.0 0.919 0.291 0.060 13.0 16.0 10
Breadth of bottom aperture 9.20 10.0 1.033 0.327 0.112 8.0 10.0 10
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Address for correspondence: Nataly Snegovaya. Laboratory of Protistology, Institute of Zoology, Azerbaijan National Academy of Sciences, Baku, AZ1073, Azerbaijan, e-mail: snegovaya@yahoo.com
